Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,686,929 G→T 50.3% Q749K (CAG→AAG)  bcsC ← cellulose synthase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,686,9290GT50.3% 3.4 / inf 223Q749K (CAG→AAG) bcsCcellulose synthase subunit
Reads supporting (aligned to +/- strand):  ref base G (55/56);  new base T (57/55);  total (112/111)
Fisher's exact test for biased strand distribution p-value = 8.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 223 total aligned reads displayed.

CTGGCGATAGAGGTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCTTCAAACTTCGCACCAT  >  NC_000913/3686831‑3687027
                                                                                                  |                                                                                                  
ctgGCGATAGAGGTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGc                                                                                                   <  1:2248005/100‑1 (MQ=255)
ctgGCGATAGAGGTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGc                                                                                                   >  2:2609644/1‑100 (MQ=255)
ctgGCGATAGAGGTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGc                                                                                                   <  1:954810/100‑1 (MQ=255)
 tgGCGATAGAGGTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCg                                                                                                  <  1:3552213/100‑1 (MQ=255)
 tgGCGATAGAGGTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCg                                                                                                  >  2:3178106/1‑100 (MQ=255)
  ggCGATAGAGGTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCgg                                                                                                 <  2:2015418/100‑1 (MQ=255)
  ggCGATAGAGGTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCgg                                                                                                 <  1:2133937/100‑1 (MQ=255)
   gCGATAGAGGTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGa                                                                                                <  1:3955218/100‑1 (MQ=255)
   gCGATAGAGGTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGa                                                                                                <  2:3319983/100‑1 (MQ=255)
       tAGAGGTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGAcgcg                                                                                            <  1:921890/100‑1 (MQ=255)
       tAGAGGTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGACCTCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGAAc                                                                                               >  1:1499160/1‑97 (MQ=255)
         gagGTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGcgtc                                                                                          >  1:4124471/1‑100 (MQ=255)
         gagGTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGcgtc                                                                                          <  1:1206290/100‑1 (MQ=255)
         gagGTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGcgtc                                                                                          >  1:502968/1‑100 (MQ=255)
            ntCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTa                                                                                       >  2:3220189/2‑100 (MQ=255)
            gTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTa                                                                                       >  1:809727/1‑100 (MQ=255)
            gTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTa                                                                                       >  1:730590/1‑100 (MQ=255)
            gTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGCCGCGTCGTa                                                                                       >  1:1000356/1‑100 (MQ=255)
             tCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAg                                                                                      <  2:3487052/100‑1 (MQ=255)
             tCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAg                                                                                      <  1:3349162/100‑1 (MQ=255)
              ccgccgCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGt                                                                                     >  1:1138062/1‑100 (MQ=255)
              ccgccgCATCGCTGCGCACGCCACGTTTAAGCCAGTCCTCTCTCTCGTCGTTACGGGTCAGGCGGGTAAAGGTGTCGTTATCCTGCGGACGAGTCGTAGt                                                                                     >  2:1812865/1‑100 (MQ=255)
                gccgcATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTca                                                                                   >  2:51212/1‑100 (MQ=255)
                 ccgcATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTcac                                                                                  <  2:2026140/100‑1 (MQ=255)
                 ccgcATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTcac                                                                                  <  1:3756272/100‑1 (MQ=255)
                  cgcATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTcaca                                                                                 <  2:3941274/100‑1 (MQ=255)
                   gcATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCAGACGCGTCGTAGTCacac                                                                                >  2:1784953/1‑100 (MQ=255)
                    cATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACAcc                                                                               <  1:2664928/100‑1 (MQ=255)
                      tCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCgg                                                                             <  2:3117853/100‑1 (MQ=255)
                         cTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATg                                                                          <  1:1370316/100‑1 (MQ=255)
                           gcgcACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCg                                                                        <  2:3713575/100‑1 (MQ=255)
                            cgcACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGa                                                                       <  1:2817092/100‑1 (MQ=255)
                             gcACGCCACGTTTCGGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACGCCGGATGCGAc                                                                      <  2:2161807/100‑1 (MQ=255)
                             gcACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGAc                                                                      >  2:732014/1‑100 (MQ=255)
                             gcACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGAc                                                                      >  1:2182146/1‑100 (MQ=255)
                                  ccACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATgg                                                                 >  1:3410455/1‑100 (MQ=255)
                                   cACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGATGCGTCGTAGTCACACCGGATGCGACCATgg                                                                 <  2:2709570/99‑1 (MQ=255)
                                   cACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGc                                                                >  1:2305098/1‑100 (MQ=255)
                                    aCGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCg                                                               <  1:3881513/100‑1 (MQ=255)
                                     cGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGt                                                              <  1:1272579/100‑1 (MQ=255)
                                      gTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTc                                                             <  1:2466356/100‑1 (MQ=255)
                                      gTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTc                                                             >  1:61221/1‑100 (MQ=255)
                                       tttCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCt                                                            >  2:725291/1‑100 (MQ=255)
                                       tttCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCt                                                            >  1:4042449/1‑100 (MQ=255)
                                         tCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCttt                                                          <  2:3039946/100‑1 (MQ=255)
                                          cAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATGCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTg                                                         >  2:3243962/1‑100 (MQ=255)
                                          cAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTg                                                         >  1:1015215/1‑100 (MQ=255)
                                             ccAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAg                                                      <  1:3189057/100‑1 (MQ=255)
                                             ccAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAg                                                      >  1:373747/1‑100 (MQ=255)
                                               aGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGt                                                    >  1:172880/1‑100 (MQ=255)
                                                  cATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTc                                                 >  2:231342/1‑100 (MQ=255)
                                                  cATCTTTCTCGTCGTTACGGGTCAGTAGGGTAATGGTGTCGTTTTCCTTCGGACGCGTCGTAGTTATACCGGATGCGACCATGGCGTCTTTGTAGGTTTc                                                 >  2:1287627/1‑100 (MQ=255)
                                                   aTCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTcc                                                <  2:2986780/100‑1 (MQ=255)
                                                        tctcGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAgcgc                                           >  2:2446548/1‑100 (MQ=255)
                                                         ctcGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGcc                                          >  1:2383067/1‑100 (MQ=255)
                                                          tcgtcgTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCGTGGCGTCTTTGTAGGTTTCCAGCGCCt                                         >  2:3089277/1‑100 (MQ=255)
                                                           cgtcgtTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTg                                        >  1:657931/1‑100 (MQ=255)
                                                           cgtcgtTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTg                                        >  1:2731955/1‑100 (MQ=255)
                                                                ttACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTg                                   <  2:2911675/100‑1 (MQ=255)
                                                                 tACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTgg                                  >  2:2932360/1‑100 (MQ=255)
                                                                 tACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTgg                                  <  2:128310/100‑1 (MQ=255)
                                                                 tACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTgg                                  <  2:3538722/100‑1 (MQ=255)
                                                                   cGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGAt                                <  1:1752858/100‑1 (MQ=255)
                                                                   cGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGCTTCCAGCGCCTGCGTTGGAt                                >  2:4031563/1‑100 (MQ=255)
                                                                    gggTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATc                               >  1:157415/1‑100 (MQ=255)
                                                                     ggTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATcg                              >  2:863858/1‑100 (MQ=255)
                                                                     ggTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATcg                              <  2:2971707/100‑1 (MQ=255)
                                                                     ggTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATcg                              <  1:899851/100‑1 (MQ=255)
                                                                     ggTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATcg                              >  1:459461/1‑100 (MQ=255)
                                                                        cAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATcgccc                           >  2:3508477/1‑100 (MQ=255)
                                                                        cAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATcgccc                           <  1:3025392/100‑1 (MQ=255)
                                                                        cAGTCGGGTAAAGGTGTCGTCATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATcgccc                           >  2:2519330/1‑100 (MQ=255)
                                                                         aGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATcgcccg                          >  1:1835067/1‑100 (MQ=255)
                                                                         aGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATcgcccg                          >  2:1906142/1‑100 (MQ=255)
                                                                          gTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATcgcccgc                         <  2:2997069/100‑1 (MQ=255)
                                                                           tCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGTGCCTGCGTTGGATcgcccgcc                        <  2:2639686/100‑1 (MQ=255)
                                                                            cGGGTAAAGGTGTCTTTCACATGCGGACGCGTCGTAGTCACACCCGATGCGACCATGGCCTCTTTGTAGGTTTCCAACGCAGGCGCTGGaaccctaaa                         <  2:2679398/98‑9 (MQ=255)
                                                                            cGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCt                       >  1:1378142/1‑100 (MQ=255)
                                                                             gggTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTg                      >  2:3032657/1‑100 (MQ=255)
                                                                             gggTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTg                      <  1:3987315/100‑1 (MQ=255)
                                                                                tAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTgcgc                   >  2:663381/1‑100 (MQ=255)
                                                                                  aaGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCtt                 >  1:279193/1‑100 (MQ=255)
                                                                                   aGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCACCCGCCTGCGCTTc                >  2:1672333/1‑100 (MQ=255)
                                                                                    ggTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCTTCa               >  1:1333844/1‑100 (MQ=255)
                                                                                      tgtCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCTTCaa              >  2:411069/1‑99 (MQ=255)
                                                                                       gtCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCTTCAAAc            <  1:301476/100‑1 (MQ=255)
                                                                                       gtCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCTTCAAAc            <  1:1062876/100‑1 (MQ=255)
                                                                                       gtCGTTATCCTTCGGACGCGTAGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCTTCAAAc            <  1:2631693/100‑1 (MQ=255)
                                                                                       gtCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCTTCAAAc            <  2:1625921/100‑1 (MQ=255)
                                                                                        tCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCTTCAAACt           <  2:2397069/100‑1 (MQ=255)
                                                                                           ttATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCTTCAAACTTCg        <  1:2326159/100‑1 (MQ=255)
                                                                                           ttATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCTTCAAACTTCg        <  1:557322/100‑1 (MQ=255)
                                                                                           ttATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCTTCAAACTTCg        <  1:2076036/100‑1 (MQ=255)
                                                                                            tATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCATCCCCTGCTTTGGATCGCCCGCCTGCGCTTCAAACTTCg        <  2:1024963/99‑1 (MQ=255)
                                                                                              tCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCTTCAAACTTCGc       <  2:1156122/98‑1 (MQ=255)
                                                                                              tCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCTTCAAACTTCGCAc     >  2:2758970/1‑100 (MQ=255)
                                                                                                cTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCTTCAAACTTCGCACCa   <  2:3590381/100‑1 (MQ=255)
                                                                                                cTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCTTCAAACTTCGCACCa   <  2:1455178/100‑1 (MQ=255)
                                                                                                 tGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCTTCAAACTTCGCACCAt  <  1:3157362/100‑1 (MQ=255)
                                                                                                 tGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCTTCAAACTTCGCACCAt  <  1:3504780/100‑1 (MQ=255)
                                                                                                  |                                                                                                  
CTGGCGATAGAGGTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCTTCAAACTTCGCACCAT  >  NC_000913/3686831‑3687027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: