Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,727,880 T→C 54.7% intergenic (‑132/+37) yiaB ← / ← xylB YiaAB family inner membrane protein/xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,727,8800TC54.7% 44.9 / 187.5 155intergenic (‑132/+37)yiaB/xylBYiaAB family inner membrane protein/xylulokinase
Reads supporting (aligned to +/- strand):  ref base T (34/36);  new base C (36/48);  total (71/84)
Fisher's exact test for biased strand distribution p-value = 5.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-01

Only 100 of 159 total aligned reads displayed.

GAGTAACCAACGAAAGACAAAACTGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGA  >  NC_000913/3727781‑3727977
                                                                                                   |                                                                                                 
gAGTAACCAACGAAAGACAAAACTGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGAtgt                                                                                                   >  2:3076429/1‑100 (MQ=255)
   tAACCAACGAAAGACAAAACTGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGaa                                                                                                <  1:1686544/100‑1 (MQ=255)
    aaCCAACGAAAGACAAAACTGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAAt                                                                                               <  2:232465/100‑1 (MQ=255)
    aaCCAACGAAAGACAAAACTGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTAAGATATATAGATGTGa                                                                                                 >  2:3687964/1‑98 (MQ=255)
      ccAACGAAAGACAAAACTGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTa                                                                                             >  1:2711903/1‑100 (MQ=255)
        aaCGAAAGACAAAACTGAAAAATGCCCTTAACAAAAGATTTTCCGAATAAATGCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATc                                                                                           >  1:3196283/1‑100 (MQ=255)
        aaCGAAAGACAAAACTGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATc                                                                                           >  1:32120/1‑100 (MQ=255)
         aCGAAAGACAAAACTGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATcc                                                                                          >  1:2071722/1‑100 (MQ=255)
          cGAAAGACAAAACTGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATctc                                                                                         <  1:2809909/100‑3 (MQ=255)
             aaGACAAAACTAAAAAATTCCAATAACAAATAATTTTCAGAATAAATTCACACTATATAATAAGTAATTACTGTGATTTATATAGGCGAATTAGcccc                                                                                        <  2:2459742/98‑1 (MQ=255)
              aGACAAAACTGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCAc                                                                                     <  1:2216605/100‑1 (MQ=255)
                aCAAAACTGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCAccc                                                                                   >  1:1547981/1‑100 (MQ=255)
                  aaaaCTGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCgg                                                                                 <  2:503390/100‑1 (MQ=255)
                   aaaCTGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATGTTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGt                                                                                >  1:3549511/1‑100 (MQ=255)
                   aaaCTGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGt                                                                                >  2:4090898/1‑100 (MQ=255)
                   aaaCTGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGt                                                                                >  2:534506/1‑100 (MQ=255)
                    aaCTGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTc                                                                               >  2:1682542/1‑100 (MQ=255)
                     aCTGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTca                                                                              <  1:593926/100‑1 (MQ=255)
                      cTGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTcag                                                                             >  1:3927071/1‑100 (MQ=255)
                      cTGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTcag                                                                             <  1:7634/100‑1 (MQ=255)
                      cTGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTcag                                                                             >  1:3719442/1‑100 (MQ=255)
                      cTGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGAGAATTATCCCCCACACGGTcag                                                                             >  1:3867064/1‑100 (MQ=255)
                       tGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTcagg                                                                            >  1:867368/1‑100 (MQ=255)
                       tGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTcagg                                                                            <  1:1158694/100‑1 (MQ=255)
                       tGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTcagg                                                                            >  1:615504/1‑100 (MQ=255)
                        gAAAAATGCCATTAAAAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATATATGCGAATTATCCACCACACGGTcag                                                                             >  2:1610539/1‑98 (MQ=255)
                            aaTGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGCAggg                                                                       <  1:3021631/100‑1 (MQ=255)
                            aaTGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGCAggg                                                                       <  2:1046516/100‑1 (MQ=255)
                            aaTGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGCAggg                                                                       <  1:482669/100‑1 (MQ=255)
                             aTGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAgggg                                                                      <  2:2184177/100‑1 (MQ=255)
                             aTGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAgggg                                                                      >  1:1133106/1‑100 (MQ=255)
                             aTGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGCAgggg                                                                      >  1:1852515/1‑100 (MQ=255)
                              tGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGa                                                                     <  2:2035743/100‑1 (MQ=255)
                                ccATTAACAAATGATTTTCAGAATAAATTCATACTTAATATTAATTAATTCCTGAGAAATATAGATTTAATTTATCCCCCACCCTGTCAGCCATGGGAt                                                                    >  2:1591654/1‑99 (MQ=255)
                                     aaCAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGtt                                                              >  2:1488805/1‑100 (MQ=255)
                                     aaCAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGtt                                                              >  2:1078072/1‑100 (MQ=255)
                                       cAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGTAGGGGATAACGTTTa                                                            >  1:3516415/1‑100 (MQ=255)
                                       cAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTa                                                            >  1:1754236/1‑100 (MQ=255)
                                       cAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTa                                                            >  1:806412/1‑100 (MQ=255)
                                         aaTGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATAATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACg                                                          >  1:2905457/1‑100 (MQ=255)
                                          aTGATTTTCAGAATAAATTCATACTACATATTAATTAATTACTGAGATATATAGATGCGAATTATCACCCACCCGGTCAGTCAGGGGATAACGTTTACGc                                                         >  2:3035819/1‑100 (MQ=255)
                                          aTGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTAcn                                                          <  2:1520234/99‑2 (MQ=255)
                                             aTTTTCAGAATAAGTTCATACTAATTATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCAt                                                      <  1:3261470/100‑1 (MQ=255)
                                             aTTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCAt                                                      <  2:2854514/100‑1 (MQ=255)
                                                ttCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTTCGCCAtt                                                     >  2:309504/1‑98 (MQ=255)
                                                 tCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCAACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAAt                                                  >  2:1550480/1‑100 (MQ=255)
                                                 tCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAAt                                                  >  1:2918892/1‑100 (MQ=255)
                                                  cAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATg                                                 <  2:70638/100‑1 (MQ=255)
                                                   aGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATATATTTGAATTATAACCCACACTGTTAAGCAGAGGATAACGTTTAAGCCATTAAtag                                                >  2:3806122/1‑98 (MQ=255)
                                                    gAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGc                                               >  2:2069154/1‑100 (MQ=255)
                                                    gAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGc                                               >  1:798354/1‑100 (MQ=255)
                                                    gAATAAATTCATACTAAATATTAATTAATTACGGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTGTACGCCATTAATGGc                                               <  2:393706/100‑1 (MQ=255)
                                                     aaTCAATTCATACTACATATTAATTAATTACTGAGATATTTAGATACGAATTATACACCAGCCTGTTAGGCAGCGGATGAATTTTACGCCATTaa                                                   >  2:1959245/1‑95 (MQ=255)
                                                     aaTAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGc                                               >  1:1362898/1‑99 (MQ=255)
                                                     aaTAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGc                                               <  2:2624499/99‑1 (MQ=255)
                                                     aaTAAATTCATACTAAATATAAATTAATTACTGACATATATAGATGTGATTTATCCCCCACCCGGTAAGGCAGGGGATAACGTATACGTCATTAATgg                                                <  2:1906608/98‑1 (MQ=255)
                                                      aTAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAg                                             <  2:1329459/100‑1 (MQ=255)
                                                      aTAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAg                                             <  2:1054436/100‑1 (MQ=255)
                                                        aaaTTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGaa                                           <  2:186351/100‑1 (MQ=255)
                                                         aaTTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGGAGGGGATAACGTTTACGCCATTAATGGCAGAAg                                          >  2:2667383/1‑100 (MQ=255)
                                                            tCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTg                                       <  2:690799/100‑1 (MQ=255)
                                                             cATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGc                                      <  2:4049009/100‑1 (MQ=255)
                                                              aTACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGc                                      <  1:4109204/99‑1 (MQ=255)
                                                              aTACTAAATATTAATTAATTACTGAGATATATAGATGCGAATGATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCt                                     <  1:346275/100‑1 (MQ=255)
                                                                aCTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGa                                   <  1:513742/100‑1 (MQ=255)
                                                                aCTAAATATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGa                                   <  1:1259277/100‑1 (MQ=255)
                                                                  tAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATa                                 >  2:1547766/1‑100 (MQ=255)
                                                                  tAAATATTAATTAATTACTGAGATATATAGAGGCGAATTATCCCCCACCCGGTCAGGCAGTGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGAt                                  <  2:814916/99‑1 (MQ=255)
                                                                    aaTATTAATTAATTACTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATaga                               <  1:3584097/100‑1 (MQ=255)
                                                                     atatTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATagag                              <  1:2814456/100‑1 (MQ=255)
                                                                      tatTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAgg                             >  2:3982254/1‑100 (MQ=255)
                                                                       attaattaattaCTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGc                            <  1:3767592/100‑1 (MQ=255)
                                                                       atgaattaattaCTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGc                            <  2:1152848/97‑1 (MQ=255)
                                                                        ttaattaattaCTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCg                           <  1:3275805/100‑1 (MQ=255)
                                                                          aattaattaCTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGGAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGAc                         <  1:2667399/100‑1 (MQ=255)
                                                                          aattaattaCTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGAc                         <  1:2386763/100‑1 (MQ=255)
                                                                          aattaattaCTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGAc                         <  2:1670045/100‑1 (MQ=255)
                                                                          aattaattaCTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGAc                         <  2:65260/100‑1 (MQ=255)
                                                                           attaattaCTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACg                        >  2:3640253/1‑100 (MQ=255)
                                                                           attaattaCTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGAGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACg                        <  2:3391182/100‑1 (MQ=255)
                                                                           attaattaCTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACg                        >  1:496314/1‑100 (MQ=255)
                                                                             taattaCTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGa                      <  1:1093591/100‑1 (MQ=255)
                                                                             taattaCTGAGATATATAGATGCGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGa                      >  1:4047081/1‑100 (MQ=255)
                                                                                   cTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGttt                >  1:842297/1‑100 (MQ=255)
                                                                                    tGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTtc               <  2:1161495/100‑1 (MQ=255)
                                                                                     gagaTATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTtct              >  1:1794830/1‑100 (MQ=255)
                                                                                     gagaTATATAGATGCGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTtct              <  1:2069167/100‑1 (MQ=255)
                                                                                       gaTATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCtcg            <  2:32120/100‑1 (MQ=255)
                                                                                       gaTATATAGATGCGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCtcg            <  2:798348/100‑1 (MQ=255)
                                                                                         tatataGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCtcgtc          >  1:1441578/1‑100 (MQ=255)
                                                                                         tatataGATGCGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCtcgtc          >  1:2351358/1‑100 (MQ=255)
                                                                                          atataGATGCGAATTATCCCCCACCCGGTCAGGCAGGGTATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCtcgtcg         <  2:3477152/100‑1 (MQ=255)
                                                                                           tataGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTcgtcgt        <  2:3253024/100‑1 (MQ=255)
                                                                                           tataGATGCGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTcgtcgt        <  1:1894145/100‑1 (MQ=255)
                                                                                            ctaGATGTGAATTATCCCCCACACGGTCAGGCAGGGGATAACGTTTACGCCCTTAATGGCAGAAGTTGCCGAAAGAGGCGACGAAACGTTCCtcgcagtg       >  2:1422389/2‑95 (MQ=255)
                                                                                             taGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTgg      <  2:1309442/100‑1 (MQ=255)
                                                                                             taGATGTGAATTATCCCCCACCCCGTCAGGCAGGGGATAACGTCTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACCGAACGTTTCTCGTCGTgg      <  2:2770286/100‑1 (MQ=255)
                                                                                              aGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGc     >  2:4133072/1‑100 (MQ=255)
                                                                                               gATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGc     <  2:1582984/99‑1 (MQ=255)
                                                                                                 tgtgAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGa  >  2:953852/1‑100 (MQ=255)
                                                                                                   |                                                                                                 
GAGTAACCAACGAAAGACAAAACTGAAAAATGCCATTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGA  >  NC_000913/3727781‑3727977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: