Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,828,936 T→C 48.4% intergenic (‑271/‑9) gltS ← / → xanP glutamate transporter/xanthine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,828,9360TC48.4% 1.3 / 217.7 159intergenic (‑271/‑9)gltS/xanPglutamate transporter/xanthine permease
Reads supporting (aligned to +/- strand):  ref base T (43/39);  new base C (44/33);  total (87/72)
Fisher's exact test for biased strand distribution p-value = 6.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

Only 100 of 162 total aligned reads displayed.

AAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGC  >  NC_000913/3828838‑3829035
                                                                                                  |                                                                                                   
aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGAtt                                                                                                    <  1:1899386/100‑1 (MQ=255)
  aGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGt                                                                                                  >  1:1108232/1‑100 (MQ=255)
    tGCATTTATCTCATTTAAATATTTAGATGCGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGtt                                                                                                 >  2:2669401/1‑99 (MQ=255)
     gCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGtttt                                                                                               >  1:3764860/1‑100 (MQ=255)
     gCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGtttt                                                                                               >  1:1744064/1‑100 (MQ=255)
         ttATCTCATTTAAATATTTAGATGAGGATGTGTCAAACGTTTGCTCTTCTCTTGCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATGGTTTTCGAt                                                                                           <  2:420758/100‑1 (MQ=255)
         ttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGAt                                                                                           <  2:587146/100‑1 (MQ=255)
          tATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATg                                                                                          >  1:674611/1‑100 (MQ=255)
          tATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATg                                                                                          <  2:807941/100‑1 (MQ=255)
          tATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATg                                                                                          >  1:3767552/1‑100 (MQ=255)
           aTCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGt                                                                                         >  2:4005734/1‑100 (MQ=255)
            tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTTAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTc                                                                                        <  2:3059654/100‑1 (MQ=255)
             ctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTc                                                                                        <  2:1912197/99‑1 (MQ=255)
               cATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGt                                                                                     >  2:1596581/1‑100 (MQ=255)
                aTTTAAATATTTAGATGAGGCTGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGtt                                                                                    >  2:2531225/1‑100 (MQ=255)
                aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGtt                                                                                    <  2:1322174/100‑1 (MQ=255)
                aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATACCCTCTTTGCTACCACTGGACTGTTTTCGATGTCTg                                                                                      >  2:236741/1‑98 (MQ=255)
                  ttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTc                                                                                  <  2:6757/100‑1 (MQ=255)
                   tAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGGTGTCTGttt                                                                                   >  2:1999230/1‑98 (MQ=255)
                     ccgatctAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCAc                                                                               <  1:3249579/94‑1 (MQ=255)
                        atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCt                                                                            >  1:578490/1‑100 (MQ=255)
                           tAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGa                                                                         >  1:2434794/1‑100 (MQ=255)
                             gATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGt                                                                       >  1:3018823/1‑100 (MQ=255)
                              aTGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGGCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTc                                                                      >  1:1781125/1‑100 (MQ=255)
                               tGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCa                                                                     >  2:348936/1‑100 (MQ=255)
                               tGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCa                                                                     >  1:3575329/1‑100 (MQ=255)
                               tGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCa                                                                     >  2:3046281/1‑100 (MQ=255)
                               tGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGt                                                                       >  2:2443705/1‑98 (MQ=255)
                                gAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAg                                                                    >  1:1593994/1‑100 (MQ=255)
                                 aGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGa                                                                   <  2:3273484/100‑1 (MQ=255)
                                 aGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGa                                                                   >  1:3296352/1‑100 (MQ=255)
                                  ggATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGaa                                                                  <  1:968290/100‑1 (MQ=255)
                                  ggATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGaa                                                                  <  1:2499058/100‑1 (MQ=255)
                                  ggATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGaa                                                                  <  2:2961344/100‑1 (MQ=255)
                                  ggATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGaa                                                                  >  1:1946378/1‑100 (MQ=255)
                                     tgtgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAAt                                                               <  1:137888/100‑1 (MQ=255)
                                        ggCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTTTTTCCACCCTCGAGTCAGAAAATgcg                                                            <  1:2510349/100‑1 (MQ=255)
                                        ggCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATgcg                                                            <  1:14958/100‑1 (MQ=255)
                                        ggCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATgcg                                                            >  1:1473607/1‑100 (MQ=255)
                                        ggCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATgcg                                                            >  1:2110329/1‑100 (MQ=255)
                                        ggCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTAGAGTCAGAAAATg                                                              >  2:3125881/1‑98 (MQ=255)
                                        ggCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCCCCCTCGAGGCAGAAAATgcg                                                            >  2:260815/1‑100 (MQ=255)
                                          cAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCa                                                          >  1:2037965/1‑100 (MQ=255)
                                             aCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAcc                                                       <  1:1841469/100‑1 (MQ=255)
                                               gTTTGCTTTTCTCTTTCGGTCAGCTGAAATGCCCGCTGTGCTACCACGGGATTGTATTCGATGTCTGTATCCACCCTCGAGTCAGAAAATGCGCGAcc                                                       >  2:316635/1‑98 (MQ=255)
                                               gTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCgg                                                     >  1:736029/1‑100 (MQ=255)
                                                  tGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTg                                                  >  1:3937695/1‑100 (MQ=255)
                                                  tGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTg                                                  >  1:1127000/1‑100 (MQ=255)
                                                  tGCTTTTCTCTTTCGGTCAGCTAAAATGCCAGCATTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCACAACCGGTTg                                                  >  2:2725341/1‑100 (MQ=255)
                                                    cTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTgcg                                                >  1:3084477/1‑100 (MQ=255)
                                                          tctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCCCCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACt                                          <  2:2967742/100‑1 (MQ=255)
                                                           ctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTc                                         >  1:342236/1‑100 (MQ=255)
                                                             ttCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCattttctg                                       <  1:2659138/100‑8 (MQ=255)
                                                             ttCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCaa                                       >  1:935474/1‑100 (MQ=255)
                                                              tCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCattttctga                                      <  1:150432/100‑9 (MQ=255)
                                                              tCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCaaa                                      <  2:3353833/100‑1 (MQ=255)
                                                                ggTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGCTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCaccac                                    >  2:2332294/1‑96 (MQ=255)
                                                                ggTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAc                                    >  2:541004/1‑100 (MQ=255)
                                                                ggTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAc                                    <  2:1168467/100‑1 (MQ=255)
                                                                 gTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTGGCGCAGACTCAAAACa                                   <  2:3370790/100‑1 (MQ=255)
                                                                   naGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGc                                 >  1:1596980/2‑100 (MQ=255)
                                                                   cAGCTAAACTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGc                                 <  2:1701954/100‑1 (MQ=255)
                                                                   cAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAg                                  >  1:3635778/1‑99 (MQ=255)
                                                                   cAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGc                                 >  1:1062673/1‑100 (MQ=255)
                                                                   cAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGCCTCTTTCCACCTTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGc                                 >  1:1437021/1‑100 (MQ=255)
                                                                    aGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCg                                >  1:3465560/1‑100 (MQ=255)
                                                                    aGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCg                                >  1:593509/1‑100 (MQ=255)
                                                                     gCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCg                                >  1:1958585/1‑99 (MQ=255)
                                                                     gCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCg                                >  1:3281326/1‑99 (MQ=255)
                                                                       tAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCg                                >  1:2292492/1‑97 (MQ=255)
                                                                       tAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAAc                             <  1:1736467/100‑1 (MQ=255)
                                                                          aaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGa                          >  1:1989858/1‑100 (MQ=255)
                                                                           aTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCCCCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGAt                         <  2:1288332/100‑1 (MQ=255)
                                                                           aTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGAt                         >  2:1741806/1‑100 (MQ=255)
                                                                           aTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGAt                         >  2:2819396/1‑100 (MQ=255)
                                                                            tGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGAtt                        <  1:1292150/100‑1 (MQ=255)
                                                                             gCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGAttt                       <  1:3397242/100‑1 (MQ=255)
                                                                             gCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGAttt                       <  1:3511290/100‑1 (MQ=255)
                                                                               ccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAc                     <  2:3781160/100‑1 (MQ=255)
                                                                               ccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAc                     >  1:1641051/1‑100 (MQ=255)
                                                                               ccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAc                     <  1:927039/100‑1 (MQ=255)
                                                                                  cTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGt                  <  2:995176/100‑1 (MQ=255)
                                                                                  cTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGt                  >  1:298216/1‑100 (MQ=255)
                                                                                   tttGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTc                 <  1:1080481/100‑1 (MQ=255)
                                                                                    ttGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGATTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCt                <  2:308997/100‑1 (MQ=255)
                                                                                     tGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCtt               <  2:736023/100‑1 (MQ=255)
                                                                                      gCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTg              >  1:518210/1‑100 (MQ=255)
                                                                                        tACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGaa            <  2:168524/100‑1 (MQ=255)
                                                                                        tACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGaa            <  1:2277677/100‑1 (MQ=255)
                                                                                         aCCACGGGATTGTTTTCGATGTCTGTTTCCCCCCTCGAGTCAGAAAATGCGAAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAg           <  2:850121/100‑1 (MQ=255)
                                                                                         aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAg           >  1:987482/1‑100 (MQ=255)
                                                                                         aCCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCTCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAg           <  2:3551026/100‑1 (MQ=255)
                                                                                         aCCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGaa            <  2:3834883/99‑1 (MQ=255)
                                                                                         aCCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAg           <  1:3254423/100‑1 (MQ=255)
                                                                                          ccACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGa          >  1:2682341/1‑100 (MQ=255)
                                                                                          ccACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGa          >  1:650653/1‑100 (MQ=255)
                                                                                          ccACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGa          >  1:2709426/1‑100 (MQ=255)
                                                                                           cACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGAt         <  1:438364/100‑1 (MQ=255)
                                                                                             cGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCg       <  1:3975404/100‑1 (MQ=255)
                                                                                                  ttGGTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccg   <  2:3575306/99‑1 (MQ=255)
                                                                                                  |                                                                                                   
AAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGC  >  NC_000913/3828838‑3829035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: