Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 400,214 T→C 16.9% D237G (GAC→GGC)  ddlA ← D‑alanine‑D‑alanine ligase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913400,2140TC16.9% 406.9 / 71.3 183D237G (GAC→GGC) ddlAD‑alanine‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base T (86/66);  new base C (19/12);  total (105/78)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

Only 100 of 186 total aligned reads displayed.

ACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTGCGT  >  NC_000913/400115‑400313
                                                                                                   |                                                                                                   
aCTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGt                                                                                                     >  2:2973162/1‑100 (MQ=255)
aCTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGt                                                                                                     <  1:2402858/100‑1 (MQ=255)
  tACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCg                                                                                                   <  2:166630/100‑1 (MQ=255)
  tACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTAAGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCg                                                                                                   >  2:2339033/1‑100 (MQ=255)
  tACCACTTTCGCGCCATCTTCGTCAATGGACTTGGGGTCGTAGGCATAGAAATCGCTGGTGAGTACTATCTCTCCACAGGTGCTGGCTTGCGGATTGTCg                                                                                                   <  2:2456043/100‑1 (MQ=255)
   aCCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGt                                                                                                  >  1:2989875/1‑100 (MQ=255)
   aCCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGt                                                                                                  >  1:4039635/1‑100 (MQ=255)
      aCTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGc                                                                                               <  1:718940/100‑1 (MQ=255)
      aCTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGc                                                                                               >  1:3848751/1‑100 (MQ=255)
         ttCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCa                                                                                            <  1:2071677/100‑1 (MQ=255)
         ttCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCa                                                                                            >  1:2243720/1‑100 (MQ=255)
            gcgcCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGaa                                                                                         >  2:1899631/1‑100 (MQ=255)
            gcgcCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGaa                                                                                         >  1:1546818/1‑100 (MQ=255)
                 aTCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTgcg                                                                                    >  1:3308113/1‑100 (MQ=255)
                  tCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTgcgc                                                                                   >  1:2343750/1‑100 (MQ=255)
                   cTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCa                                                                                  <  1:3162368/100‑1 (MQ=255)
                   cTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCa                                                                                  <  1:2973180/100‑1 (MQ=255)
                    ttCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCAt                                                                                 <  1:1503046/100‑1 (MQ=255)
                         cAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGAt                                                                            <  2:2014034/100‑1 (MQ=255)
                          aaTGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGAtc                                                                           <  1:735513/100‑1 (MQ=255)
                            tGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGAtctc                                                                         >  1:1590665/1‑100 (MQ=255)
                              tACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCAc                                                                       >  1:3095237/1‑100 (MQ=255)
                              tACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCAc                                                                       <  1:1108929/100‑1 (MQ=255)
                              tACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCAc                                                                       >  2:1463055/1‑100 (MQ=255)
                                cTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGa                                                                     <  2:3383602/100‑1 (MQ=255)
                                 ttGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGAc                                                                    <  2:3456409/100‑1 (MQ=255)
                                    gtgtCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCtt                                                                 <  1:3964837/100‑1 (MQ=255)
                                    gtgtCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGATGCCCAGAACTGCGCATTCGATCTCACGACCtt                                                                 <  2:2485176/100‑1 (MQ=255)
                                     tgtCGTAGGCATAGGAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGACCTCACGACCgtt                                                                <  2:3064539/100‑1 (MQ=255)
                                     tgtCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCttt                                                                <  1:1623847/100‑1 (MQ=255)
                                      gtCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTg                                                               >  1:1656321/1‑100 (MQ=255)
                                      gtCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCACATTCGATCTCACGACCTTTg                                                               >  1:139668/1‑100 (MQ=255)
                                      gtCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGCTGCCCAGAACTGCGCATTCGATCTCACGACCTTTg                                                               >  2:1135136/1‑100 (MQ=255)
                                      ggcGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCttt                                                                <  2:2131014/97‑1 (MQ=255)
                                        cGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGAt                                                             >  1:133325/1‑100 (MQ=255)
                                         gTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATc                                                            >  1:1196241/1‑100 (MQ=255)
                                            ggCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCt                                                         >  1:772929/1‑100 (MQ=255)
                                            ggCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCt                                                         >  2:448689/1‑100 (MQ=255)
                                            ggCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCt                                                         >  2:3721251/1‑100 (MQ=255)
                                             gCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCtt                                                        <  2:773968/100‑1 (MQ=255)
                                             gCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCtt                                                        >  2:2380484/1‑100 (MQ=255)
                                              cATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTTCGCATTCGATCTCACGACCTTTGATCCCTTg                                                       >  2:1397148/1‑100 (MQ=255)
                                              cATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTg                                                       >  2:2530204/1‑100 (MQ=255)
                                              cATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTg                                                       >  1:1257726/1‑100 (MQ=255)
                                              cATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTg                                                       >  1:1794727/1‑100 (MQ=255)
                                              cATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTg                                                       >  2:1546599/1‑100 (MQ=255)
                                               aTAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGAATGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGc                                                      <  1:3140247/100‑1 (MQ=255)
                                                tAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGCTCTCACGACCTTTGATCCCTTGCt                                                     >  1:1871846/1‑100 (MQ=255)
                                                 aGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGc                                                      <  2:2847942/98‑1 (MQ=255)
                                                 aGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTc                                                    <  2:100060/100‑1 (MQ=255)
                                                  gAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCa                                                   <  2:1731566/100‑1 (MQ=255)
                                                   aaaTCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCaa                                                  >  2:2095605/1‑100 (MQ=255)
                                                     aTCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACg                                                <  1:3810208/100‑1 (MQ=255)
                                                      tCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGa                                               >  1:1721861/1‑100 (MQ=255)
                                                       cGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGAt                                              >  2:2623618/1‑100 (MQ=255)
                                                        gCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATc                                             <  2:3122649/100‑1 (MQ=255)
                                                        gCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATc                                             <  1:1062858/100‑1 (MQ=255)
                                                        gCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATc                                             <  1:3684988/100‑1 (MQ=255)
                                                         cTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACTACCTTTGATCCCTTGCTCAACGATCa                                            <  1:2522875/100‑1 (MQ=255)
                                                         cTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCa                                            <  1:81867/100‑1 (MQ=255)
                                                         cTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCa                                            >  1:3391583/1‑100 (MQ=255)
                                                         cTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCa                                            <  2:2065833/100‑1 (MQ=255)
                                                          tGGTTAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCAc                                           <  1:1016889/100‑1 (MQ=255)
                                                            gTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACtt                                         <  1:3516349/100‑1 (MQ=255)
                                                                gTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATg                                     >  2:511267/1‑100 (MQ=255)
                                                                gTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATg                                     >  1:748548/1‑100 (MQ=255)
                                                                  aCGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAt                                   >  2:895671/1‑100 (MQ=255)
                                                                  aCGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAt                                   >  1:246810/1‑100 (MQ=255)
                                                                   cGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtc                                  >  1:3853921/1‑100 (MQ=255)
                                                                    gATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcg                                 <  1:2844356/100‑1 (MQ=255)
                                                                    gATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcg                                 >  1:3638898/1‑100 (MQ=255)
                                                                    gATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcg                                 <  2:3814214/100‑1 (MQ=255)
                                                                     aTCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcga                                >  1:1439192/1‑100 (MQ=255)
                                                                       ctctcCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaac                              <  1:27638/100‑1 (MQ=255)
                                                                       ctctcCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaac                              <  2:1928978/100‑1 (MQ=255)
                                                                        tctcCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaact                             >  1:794772/1‑100 (MQ=255)
                                                                        tctcCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaact                             >  2:2137293/1‑100 (MQ=255)
                                                                         ctcCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactc                            >  1:3514431/1‑100 (MQ=255)
                                                                         ctcCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaact                             <  2:2732985/99‑1 (MQ=255)
                                                                          tcCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcg                           <  2:618781/100‑1 (MQ=255)
                                                                           ccACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcga                          >  1:1722689/1‑100 (MQ=255)
                                                                             acaGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcgaac                        <  1:1282460/100‑1 (MQ=255)
                                                                              ccggTGCTTGCTTGCAGATTGTCGTTGCCCAGAACTGCGCATTCTATCTCACGACCTTTGATCCCTTGCTCATCGATCACTTTATGATCGAACTCGAACg                       <  2:75770/98‑1 (MQ=255)
                                                                                 gTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCa                    <  1:3778807/100‑1 (MQ=255)
                                                                                 gTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCa                    <  1:657180/100‑1 (MQ=255)
                                                                                   gCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGa                  >  2:4067940/1‑100 (MQ=255)
                                                                                    cTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGAt                 >  2:868102/1‑100 (MQ=255)
                                                                                    cTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGAt                 >  2:333943/1‑100 (MQ=255)
                                                                                     tGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATc                >  1:3718538/1‑100 (MQ=255)
                                                                                     tGGCTTGCGGATGGCCGTTGCCCAGAACTGCGCATTCGATCTCCCGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATc                <  2:2643242/100‑1 (MQ=255)
                                                                                        catGCGGATTGTCATTGCCCAGAACTGCGCAATCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGAc             >  1:2788104/3‑100 (MQ=255)
                                                                                        cTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGAc             >  1:563506/1‑100 (MQ=255)
                                                                                        cTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGAc             >  2:2574449/1‑100 (MQ=255)
                                                                                         ttGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACg            <  2:3635358/100‑1 (MQ=255)
                                                                                           gCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGc          <  2:3141551/100‑1 (MQ=255)
                                                                                            cGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCa         >  2:4088536/1‑100 (MQ=255)
                                                                                                  gTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGACCGCCAGATCGACGGCAATTGCg   >  2:2607395/1‑100 (MQ=255)
                                                                                                  gTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGTTCGACGGCAATTGc    >  1:4070913/1‑99 (MQ=255)
                                                                                                  gTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTGCg   >  1:2281568/1‑100 (MQ=255)
                                                                                                   tCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTGCGt  <  2:246809/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
ACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTGCGT  >  NC_000913/400115‑400313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑