Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,950,468 G→T 100% intergenic (+48/‑39) ilvL → / → ilvX ilvG operon leader peptide/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,950,4680GT98.9% 556.8 / ‑3.0 171intergenic (+48/‑39)ilvL/ilvXilvG operon leader peptide/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (80/89);  total (81/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01

Only 100 of 178 total aligned reads displayed.

GATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAA  >  NC_000913/3950372‑3950564
                                                                                                 |                                                                                                
gATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGtttt                                                                                               <  2:1544639/100‑1 (MQ=255)
   tattatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTtttttt                                                                                            >  2:1655190/1‑100 (MQ=255)
    attatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGtttttttt                                                                                           <  1:3739275/100‑1 (MQ=255)
     ttatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTtttttttt                                                                                          <  1:2480022/100‑1 (MQ=255)
       atCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACTAACTAAGACCGCCGCACCGAAAGGTCCGTGGTTTTTTTTTGa                                                                                        <  1:2788806/100‑1 (MQ=255)
       atCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGa                                                                                        >  1:2152769/1‑100 (MQ=255)
         cccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGTcc                                                                                      >  1:3808361/1‑100 (MQ=255)
         cccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGAcc                                                                                      <  1:1220343/100‑1 (MQ=255)
         cccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGAcc                                                                                      >  1:3940355/1‑100 (MQ=255)
         cccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGAcc                                                                                      <  2:4114064/100‑1 (MQ=255)
          ccaccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCggggttttttttttgacc                                                                                     <  1:3295350‑M2/95‑6 (MQ=255)
          ccaccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCt                                                                                     >  2:1507273‑M2/6‑100 (MQ=255)
          ccaccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCt                                                                                     >  1:1069917‑M2/6‑100 (MQ=255)
          ccaccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCt                                                                                     >  1:1930272‑M2/6‑100 (MQ=255)
            accGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTa                                                                                   <  1:3489148‑M2/97‑1 (MQ=255)
            accGTGCGGGGCTGCACTTCGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTa                                                                                   <  2:4037719‑M2/97‑1 (MQ=255)
             ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTaa                                                                                  >  1:328240‑M2/3‑100 (MQ=255)
             ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTaa                                                                                  >  1:3173947‑M2/3‑100 (MQ=255)
              cGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTaaa                                                                                 <  1:2953207‑M2/99‑1 (MQ=255)
               gTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTTACCtt                                                                                    >  2:3413460/1‑96 (MQ=255)
               gTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGCACTTa                                                                                   >  2:3820296/1‑97 (MQ=255)
                 gCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTaaaaa                                                                               <  2:3759482/99‑1 (MQ=255)
                     ggCTGCACTTGGACGAGGAAAGGCTTAGAGATCCAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACa                                                                             <  2:2493998/97‑1 (MQ=255)
                      gCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAAc                                                                         <  2:1318671/100‑1 (MQ=255)
                      gCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAAc                                                                         >  1:159892/1‑100 (MQ=255)
                       cTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTTTCCTTAAAAACaata                                                                          >  2:3874325/1‑95 (MQ=255)
                        tGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCg                                                                       <  1:2082923/100‑1 (MQ=255)
                           aCTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCgagg                                                                    <  2:3134538/100‑1 (MQ=255)
                           aCTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCgagg                                                                    >  1:679378/1‑100 (MQ=255)
                            cTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTTGACCTAAAAAAATAAACgaag                                                                    >  1:232802/1‑97 (MQ=255)
                              tGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGc                                                                 <  2:1959182/100‑1 (MQ=255)
                              tGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGc                                                                 >  2:1100229/1‑100 (MQ=255)
                               ggACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGc                                                                 <  2:1232208/99‑1 (MQ=255)
                               ggACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCa                                                                >  1:840476/1‑100 (MQ=255)
                               ggACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCa                                                                >  1:115656/1‑100 (MQ=255)
                                gACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAg                                                               <  1:698777/100‑1 (MQ=255)
                                 aCGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                              <  1:2001123/100‑1 (MQ=255)
                                  cGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTTTCCTTTTAATCATATCCGAGGAGCAGAc                                                             >  2:1133449/1‑100 (MQ=255)
                                  cGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACAGAACCGAGGAGCAGAc                                                             >  1:4067245/1‑100 (MQ=255)
                                     ggAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAAt                                                          <  2:3193380/100‑1 (MQ=255)
                                     ggAAAGGCTTAGAGATCAAGCCTGAACAAACAAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAAt                                                          <  1:6947/100‑1 (MQ=255)
                                      gAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATg                                                         >  1:1493287/1‑100 (MQ=255)
                                      gAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATg                                                         <  1:3864532/100‑1 (MQ=255)
                                       aaaGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGa                                                        >  1:3124212/1‑100 (MQ=255)
                                          ggCTTAGAGATTAAGCCTTAACGAACTAAGACCCCGGGACCAAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATa                                                     <  2:1152163/100‑1 (MQ=255)
                                            cTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAAc                                                   >  1:1041007/1‑100 (MQ=255)
                                            cTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAAc                                                   >  2:357177/1‑100 (MQ=255)
                                             ttAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACa                                                  <  1:3656137/100‑1 (MQ=255)
                                             ttAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACa                                                  >  1:3378131/1‑100 (MQ=255)
                                              tAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAg                                                 <  1:3188556/100‑1 (MQ=255)
                                               agagATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAATCGAGGAGCAGACAATGAATAACAGc                                                >  2:683892/1‑100 (MQ=255)
                                                 agaTCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGcac                                              <  2:4067089/100‑1 (MQ=255)
                                                    ttaaGCCGTAACGAACTACGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAAt                                                      <  2:2191148/88‑1 (MQ=255)
                                                    tCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGTTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACaaa                                           >  1:1854631/1‑100 (MQ=255)
                                                    tCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACaaa                                           >  1:2533486/1‑100 (MQ=255)
                                                        gcgttAACGAACTAAGACCCCCGCACCGAAAGGCCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTc                                       <  2:1859424/97‑1 (MQ=255)
                                                        gCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTc                                       >  2:1009247/1‑100 (MQ=255)
                                                         ccTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTc                                       >  1:1056249/1‑99 (MQ=255)
                                                         ccTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCt                                      <  1:3039613/100‑1 (MQ=255)
                                                         ccTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCt                                      >  1:2632817/1‑100 (MQ=255)
                                                           ttAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGt                                    <  2:1166857/100‑1 (MQ=255)
                                                             aaCGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGttt                                  <  1:1664059/100‑1 (MQ=255)
                                                              aCGAACTAAGACCCCCGCACCGAGAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtc                                 <  1:3531747/100‑1 (MQ=255)
                                                              aCGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtc                                 <  2:2936790/100‑1 (MQ=255)
                                                              aCGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtc                                 >  1:458744/1‑100 (MQ=255)
                                                               cgtaCTAAGACCCCCGCACCGAAATTTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtct                                <  2:3656730/97‑1 (MQ=255)
                                                                gAACTAAGAACCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtc                                 <  2:519046/98‑1 (MQ=255)
                                                                 aaCTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCa                              >  2:3434619/1‑100 (MQ=255)
                                                                 aaCTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCa                              <  2:3163555/100‑1 (MQ=255)
                                                                 aaCTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCa                              >  1:3695941/1‑100 (MQ=255)
                                                                 aaCGAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCa                              <  2:2476824/100‑1 (MQ=255)
                                                                  aCTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAACATTCTGTTTCTCa                              >  1:496834/1‑99 (MQ=255)
                                                                   cTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAg                            <  2:72376/100‑1 (MQ=255)
                                                                    tAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGa                           <  1:2766033/100‑1 (MQ=255)
                                                                    tAAGACCCCCGCACCGAAAGGGCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCaa                             <  1:1103692/98‑1 (MQ=255)
                                                                     aaGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGAt                          >  2:1925542/1‑100 (MQ=255)
                                                                     aaGACCCCCGCACCGAAAGGGCCGAGGCTTTTTTTGACCTTAAAAACATAACCAAGGAGCCGACACAGAATCGTAGCTCAAAAtccagacg                                  >  2:3903442/1‑84 (MQ=255)
                                                                       gACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTc                        >  1:1718742/1‑100 (MQ=255)
                                                                         cccccGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAg                      >  1:2581673/1‑100 (MQ=255)
                                                                         cccccGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAg                      >  1:785058/1‑100 (MQ=255)
                                                                          ccccGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAgg                     <  2:2034695/100‑1 (MQ=255)
                                                                           cccGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGa                    >  1:1263459/1‑100 (MQ=255)
                                                                           cccGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGa                    <  1:1833243/100‑1 (MQ=255)
                                                                            ccGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGAc                   >  1:1752184/1‑100 (MQ=255)
                                                                                aCCGAAAGGTCCGGGGTTTTTTTTTTTTTTTTTTAAACATAAACGAGGAGCAGACAATCAATTATTGCTTAAAATTCTGTTTTTGAAGTTTCAGGa                    >  2:2966715/1‑96 (MQ=255)
                                                                                aCCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACgggg               <  1:1545441/99‑1 (MQ=255)
                                                                                 ccGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACgggg               >  1:3729674/1‑99 (MQ=255)
                                                                                 ccGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGa              <  1:2948800/100‑1 (MQ=255)
                                                                                 ccGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGa              <  2:2434760/100‑1 (MQ=255)
                                                                                   gAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaac            >  1:1045392/1‑100 (MQ=255)
                                                                                   gAAAGGTCCGGGGTTTTTTTTTTTCTTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGcac            >  1:503075/1‑100 (MQ=255)
                                                                                   gAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaac            >  1:3005219/1‑100 (MQ=255)
                                                                                   gAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaac            >  1:549848/1‑100 (MQ=255)
                                                                                   gAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaac            <  2:2000035/100‑1 (MQ=255)
                                                                                         tCCGGGGTTTTTTTTTTTTTTTTTAAACATAACCGAGGAGCACACAATTCATCACAGCCCAGAATTCTGTTTCTTAAGATTCAGGAcccgccacta          >  2:1831272/1‑87 (MQ=255)
                                                                                         tCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAactaacta      >  1:1776240/1‑100 (MQ=255)
                                                                                          ccGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAATATTCAGGACGGGGAactaacta      <  2:2395151/99‑1 (MQ=255)
                                                                                          ccGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTAt     <  2:3464863/100‑1 (MQ=255)
                                                                                          ccGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTAt     >  2:3209345/1‑100 (MQ=255)
                                                                                             gggtTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGaa  <  1:2568143/100‑1 (MQ=255)
                                                                                                 |                                                                                                
GATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAA  >  NC_000913/3950372‑3950564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: