Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,168,540 T→A 100% intergenic (+93/‑101) gltT → / → rrlB tRNA‑Glu/23S ribosomal RNA of rrnB operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,168,5400TA99.2% 576.3 / ‑5.0 216intergenic (+93/‑101)gltT/rrlBtRNA‑Glu/23S ribosomal RNA of rrnB operon
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base A (123/91);  total (124/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 216 total aligned reads displayed.

CGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTT  >  NC_000913/4168443‑4168636
                                                                                                 |                                                                                                
  ccACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAg                                                                                              >  2:2776147/1‑100 (MQ=255)
   cACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGc                                                                                             <  2:159702/100‑1 (MQ=255)
   cACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGc                                                                                             <  2:2110763/100‑1 (MQ=255)
    aCTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCt                                                                                            <  1:951302/100‑1 (MQ=255)
         cTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGaaaa                                                                                       >  2:3574770/1‑100 (MQ=255)
           ggTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAAtt                                                                                     <  1:2283817/100‑1 (MQ=255)
           ggTTTGTGAGTGAAAGTCGCCGACCGGAATATGTCAAAACTGTTGTTCGGGCGATGTGTGAGATATTTGCTGTTTAAAAATCTGGAACAAGCTGa                                                                                          >  2:3503174/1‑95 (MQ=14)
            gTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTg                                                                                    <  2:3650305/100‑1 (MQ=255)
            gTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTg                                                                                    >  1:741285/1‑100 (MQ=255)
            gTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTg                                                                                    <  1:1273037/100‑1 (MQ=255)
             tttGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGa                                                                                   <  2:202517/100‑1 (MQ=255)
              ttGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGaa                                                                                  >  2:1882218/1‑100 (MQ=255)
              ttGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGaa                                                                                  <  1:4072715/100‑1 (MQ=255)
              ttGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCCCTTTACAAATCTGGATCAAGCTGAAAATTGa                                                                                   >  2:1199448/1‑99 (MQ=255)
              gtgtgAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGaa                                                                                  >  2:2497563/2‑100 (MQ=255)
               tgtgAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGaaa                                                                                 <  1:3105484/100‑1 (MQ=255)
                gtgagtgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAac                                                                                >  2:3675225/1‑100 (MQ=255)
                gtgagtgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAac                                                                                <  1:3910007/100‑1 (MQ=255)
                  gagtgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAacac                                                                              >  1:551648/1‑100 (MQ=255)
                  gagtgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAacac                                                                              >  2:1051896/1‑100 (MQ=255)
                   agtgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACt                                                                             >  2:3341454/1‑100 (MQ=255)
                   agtgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACt                                                                             >  1:2395402/1‑100 (MQ=255)
                   agtgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACt                                                                             >  1:931342/1‑100 (MQ=255)
                    gtgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTg                                                                            <  1:6299/100‑1 (MQ=255)
                     tgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGATGAAAGTTGAAACACTGa                                                                           <  2:1068643/100‑1 (MQ=255)
                      gaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGaa                                                                          >  2:3976819/1‑100 (MQ=255)
                      gaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGaa                                                                          >  1:1521944/1‑100 (MQ=255)
                       aaaGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATATTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGaac                                                                         <  2:2650333/100‑1 (MQ=255)
                        aaGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAACCACTGaaca                                                                        >  2:1467012/1‑100 (MQ=255)
                         aGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGaacaa                                                                       >  2:2372860/1‑100 (MQ=255)
                          gTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAGACACTGaacaac                                                                      <  1:2712963/100‑1 (MQ=255)
                          gTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGaacaac                                                                      >  2:1437486/1‑100 (MQ=255)
                            cGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACga                                                                    >  1:1196859/1‑100 (MQ=25)
                             gCCGACTTTGATATCTCAAAACTCATCTTCGGGTTATGCTTGAGATATTTGCTCTTTAAAAATCTTGAACAAGCTGAAAATTGAAACACTGAACAACgag                                                                   <  2:3139695/100‑1 (MQ=11)
                             gCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACgag                                                                   >  1:3753383/1‑100 (MQ=25)
                              ccGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACgaga                                                                  <  2:2683736/100‑1 (MQ=25)
                              ccGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACgaga                                                                  <  2:2801179/100‑1 (MQ=25)
                               cGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATCGAAACACTGAACAACGagag                                                                 <  2:2525356/100‑1 (MQ=255)
                                 aCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAgtt                                                               <  2:1672206/100‑1 (MQ=18)
                                  ccTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAggtg                                                              >  2:965726/1‑100 (MQ=12)
                                    ttAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAgttgtt                                                            >  1:516185/1‑100 (MQ=18)
                                     tAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTc                                                           >  2:1047389/1‑100 (MQ=18)
                                        tatCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTg                                                        <  2:2759784/100‑1 (MQ=18)
                                        tatCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTg                                                        >  2:1923107/1‑100 (MQ=18)
                                        tatCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTg                                                        <  2:1753826/100‑1 (MQ=18)
                                        tatCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTg                                                        <  2:2348423/100‑1 (MQ=18)
                                        tatCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTGTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTg                                                        <  2:2872027/100‑1 (MQ=16)
                                         atCTCAAAACTCATCTTCGGGTGATGTTTGAGAAAAAAAAAAAAAAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGa                                                       <  2:445780/100‑1 (MQ=11)
                                          tctcAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAg                                                      <  1:3561161/100‑1 (MQ=18)
                                          tctcAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAg                                                      <  2:846441/100‑1 (MQ=18)
                                          tctcAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAg                                                      <  2:1618833/100‑1 (MQ=18)
                                           ctcAAAACTCATCTTCGGGTGATGTTTGAGATGTTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGt                                                     >  1:3920239/1‑100 (MQ=255)
                                           ctcAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGt                                                     >  1:2366861/1‑100 (MQ=18)
                                            tcAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGtc                                                    >  1:3974476/1‑100 (MQ=18)
                                            tcAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGtc                                                    >  2:752886/1‑100 (MQ=18)
                                            tcAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGt                                                     >  2:1718263/1‑99 (MQ=17)
                                                aaCTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGtctctc                                                <  2:3918885/100‑1 (MQ=17)
                                                  cTCATCTTCGGGTGATGTTTGAGATATTGGCTCTTTCAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCaa                                              >  2:895834/1‑100 (MQ=255)
                                                    cATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAAt                                            >  2:83875/1‑100 (MQ=17)
                                                         tCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCg                                       >  2:544296/1‑100 (MQ=18)
                                                         tCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCg                                       <  2:512520/100‑1 (MQ=18)
                                                          cGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGTGTTGTTCGTGAGTCTCTCAAATTTTCGc                                      >  2:3020342/1‑100 (MQ=12)
                                                           gggTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCa                                     >  2:126879/1‑100 (MQ=18)
                                                           gggTGATGTTTGAGATATTTGATATTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCa                                     <  2:3845074/100‑1 (MQ=255)
                                                           gggTGATGTTGGAGATATTTGCTCTTTAAAAATCTGGAAGAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGc                                      <  2:624531/99‑1 (MQ=255)
                                                             gTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAac                                   >  2:2231640/1‑100 (MQ=18)
                                                              tGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAaca                                  >  1:1950383/1‑100 (MQ=18)
                                                              tGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAaca                                  <  1:3675249/100‑1 (MQ=18)
                                                                  gTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACgat                              >  1:1319943/1‑100 (MQ=17)
                                                                    ttGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACgatga                            >  1:2584929/1‑100 (MQ=18)
                                                                    ttGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAAGTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACgatga                            >  2:2255512/1‑100 (MQ=12)
                                                                      gagaTATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGatgatg                          <  1:788315/100‑1 (MQ=18)
                                                                      gagaTATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGatgatg                          <  1:4133859/100‑1 (MQ=18)
                                                                       agaTATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGGTCGTGAGTCTCTCAAATTTTCGCAACACGAcgatga                         <  2:4076143/100‑1 (MQ=255)
                                                                        gaTATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGAtgatga                         >  1:109437/1‑99 (MQ=18)
                                                                        gaTATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGaa                        >  1:2747033/1‑100 (MQ=18)
                                                                        gaTATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGaa                        >  2:2299202/1‑100 (MQ=17)
                                                                          tatTTGCTCTTTAAAAATGTGGAACGAGCTGAAATTTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATc                      <  2:2176330/100‑1 (MQ=11)
                                                                          tatTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATc                      >  1:3013624/1‑100 (MQ=18)
                                                                          tatTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATc                      <  1:595260/100‑1 (MQ=18)
                                                                          tatTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATc                      >  1:1173091/1‑100 (MQ=17)
                                                                          tatTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTATCAAATTTTCGCAACACGATGATTAATc                      >  2:549724/1‑100 (MQ=255)
                                                                          tatTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATCGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATc                      >  1:2919905/1‑100 (MQ=255)
                                                                              tGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaa                  <  2:373137/100‑1 (MQ=18)
                                                                              tGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaa                  >  2:816838/1‑100 (MQ=18)
                                                                               gCTCTTTAAAAATCTGGAACAAGCTGGAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaag                 >  1:1796286/1‑100 (MQ=12)
                                                                                ctctTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaaga                <  1:2418959/100‑1 (MQ=18)
                                                                                ctctTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaaga                <  1:3338337/100‑1 (MQ=18)
                                                                                 tctTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaagaa               >  1:30484/1‑100 (MQ=17)
                                                                                  ctTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaagaaa              <  1:2753248/100‑1 (MQ=18)
                                                                                   tttAAAAATCTGGAACAAGCTGAAGATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTATCAAATTTTCGCAACACGATGATGAATCgaaagaaa              <  2:3355023/99‑1 (MQ=255)
                                                                                     tAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACAt           >  1:828137/1‑100 (MQ=17)
                                                                                     tAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACAt           >  1:4029536/1‑100 (MQ=18)
                                                                                     tAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCGCAAATTTTAGCAACACGATGATGAATCGAAAGAAACAt           >  2:2617167/1‑100 (MQ=255)
                                                                                       aaaaTCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCt         >  1:2529569/1‑100 (MQ=18)
                                                                                       aaaaTCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTATCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCt         >  2:1223508/1‑100 (MQ=12)
                                                                                            cTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCggg    >  1:104861/1‑100 (MQ=18)
                                                                                             tGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGt   >  1:3852995/1‑100 (MQ=18)
                                                                                              ggAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGtt  >  1:2627160/1‑100 (MQ=18)
                                                                                              ggAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGtt  >  1:3497564/1‑100 (MQ=18)
                                                                                                 |                                                                                                
CGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTT  >  NC_000913/4168443‑4168636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: