Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,279,370 G→T 24.7% A298S (GCA→TCA)  ghxP → guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,279,3700GT24.7% 382.5 / 131.1 200A298S (GCA→TCA) ghxPguanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base G (69/80);  new base T (18/31);  total (88/112)
Fisher's exact test for biased strand distribution p-value = 2.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

Only 100 of 202 total aligned reads displayed.

GTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGC  >  NC_000913/4279271‑4279468
                                                                                                   |                                                                                                  
gTGATGACGGGAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAg                                                                                                    <  2:3252656/100‑1 (MQ=255)
gTGATGACGGCAGTATTTGATGCCACCGGAACTATCCATGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAg                                                                                                    >  2:2202688/1‑100 (MQ=255)
 tgatgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGc                                                                                                   >  2:968273/1‑100 (MQ=255)
 tgatgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGc                                                                                                   >  1:1343/1‑100 (MQ=255)
 tgatgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGc                                                                                                   >  2:1942880/1‑100 (MQ=255)
 tgatgaCGGCAGTATTTGATGCCACCGGAACTATCCATGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGc                                                                                                   <  2:3625557/100‑1 (MQ=255)
   atgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcac                                                                                                 <  2:444876/100‑1 (MQ=255)
   atgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcac                                                                                                 >  1:2158827/1‑100 (MQ=255)
    tgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcact                                                                                                >  2:3166728/1‑100 (MQ=255)
    tgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcact                                                                                                >  1:1622271/1‑100 (MQ=255)
          cAGTATTTGATGCCACCGGAGCTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgaccac                                                                                          >  1:3890359/1‑100 (MQ=255)
          cAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgaccac                                                                                          >  1:1653500/1‑100 (MQ=255)
           aGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgaccact                                                                                         <  2:60014/100‑1 (MQ=255)
           aGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgaccact                                                                                         <  2:3158677/100‑1 (MQ=255)
           aGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgaccact                                                                                         <  1:3304562/100‑1 (MQ=255)
            gTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgaccactg                                                                                        <  1:370806/100‑1 (MQ=255)
            gTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgaccactg                                                                                        <  1:3669614/100‑1 (MQ=255)
              aTTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgaccactgac                                                                                      >  2:2531179/1‑100 (MQ=255)
                ttGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTc                                                                                    >  1:2826471/1‑100 (MQ=255)
                 tGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGGCGGTGGGAAAGCACTGACCACTGACTcc                                                                                   >  2:813872/1‑100 (MQ=255)
                 tGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTcc                                                                                   <  1:1222722/100‑1 (MQ=255)
                  gATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCa                                                                                  >  1:484759/1‑100 (MQ=255)
                   aTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCAt                                                                                 <  2:3547768/100‑1 (MQ=255)
                    tGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGCTCATCGACGGTGGGAAAGCACTGACCACTGACTCCAt                                                                                 >  2:3121932/1‑99 (MQ=255)
                    tGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATg                                                                                <  2:3195538/100‑1 (MQ=255)
                    tGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATg                                                                                <  1:1379331/100‑1 (MQ=255)
                    tGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATg                                                                                <  2:1866007/100‑1 (MQ=255)
                     gCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGa                                                                               <  1:2882963/100‑1 (MQ=255)
                     gCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGa                                                                               <  1:4041406/100‑1 (MQ=255)
                      ccACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGag                                                                              <  1:3457117/100‑1 (MQ=255)
                      ccACCGGAACTATCCGTGCCGTCGCCGGCCACGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAACGTACTGACCACTCACTCCATGag                                                                              >  2:3246425/1‑100 (MQ=38)
                       cACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGagc                                                                             <  1:573644/100‑1 (MQ=255)
                         tccgatcTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGagcag                                                                           <  1:3000035/94‑1 (MQ=255)
                          cGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGagcagc                                                                          <  1:457459/100‑1 (MQ=255)
                           ggAACTATCCGCGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCg                                                                         <  1:2250621/100‑1 (MQ=255)
                              aCTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGttt                                                                      >  1:1435666/1‑100 (MQ=255)
                                 aTCCGTGCCGTCGCCGGCCAGGTGAACCTGCTGGATAAAGATGGGCAGATCATCGAAGGTGGGAAAGCACTGAACCATGACTCCATGAGCAGCGCTTtc                                                                    >  2:330561/1‑99 (MQ=37)
                                 aTCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGCTAAAGATGGGCAGATCATCGACGGTGGGAAATgcctgaccactgacacagtg                                                                                >  2:2711600/1‑81 (MQ=25)
                                 aTCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGagcagagatcgga                                                                   >  2:3000016/1‑92 (MQ=255)
                                 aTCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTtct                                                                   >  2:2032987/1‑100 (MQ=255)
                                 aTCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTtct                                                                   >  2:694884/1‑100 (MQ=255)
                                 aTCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTtct                                                                   >  2:3071800/1‑100 (MQ=255)
                                 aTCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTtct                                                                   >  1:863705/1‑100 (MQ=255)
                                   ccGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTctct                                                                 >  1:1724367/1‑100 (MQ=255)
                                    cGTGCCGTCGCCGGCCATGCGAACCTGCTGCATAAAGATGGGCAGATCATCGACGGTGGTAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTg                                                                <  2:517464/100‑1 (MQ=255)
                                    cGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGTAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTg                                                                <  2:2848254/100‑1 (MQ=255)
                                            cGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtggt                                                         <  2:3564553/99‑1 (MQ=255)
                                            cGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCt                                                            <  2:2570472/96‑1 (MQ=255)
                                            cGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtggtg                                                        <  1:3729532/100‑1 (MQ=255)
                                            cGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtggtg                                                        <  1:767851/100‑1 (MQ=255)
                                             gCCGGCCAGGCGAACCTGCTGGCTAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtcgtgg                                                       >  2:3328652/1‑100 (MQ=255)
                                              ccGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTggtggg                                                      <  1:2030495/100‑1 (MQ=255)
                                                 gCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGc                                                   <  1:3275502/100‑1 (MQ=255)
                                                  ccAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCg                                                  >  1:2379254/1‑100 (MQ=255)
                                                   cAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCg                                                  <  2:2280222/99‑1 (MQ=255)
                                                       cGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTcc                                             >  2:258901/1‑100 (MQ=255)
                                                        gAACCGGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTcc                                             <  2:1608099/99‑1 (MQ=255)
                                                         aaCCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCgg                                           <  2:4023268/100‑1 (MQ=255)
                                                          aCCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGc                                          <  1:2448386/100‑1 (MQ=255)
                                                             tgctgGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGc                                       >  2:842486/1‑100 (MQ=255)
                                                              gctgGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCg                                      <  1:1706083/100‑1 (MQ=255)
                                                              gctgGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCg                                      <  1:2567204/100‑1 (MQ=255)
                                                              gctgGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCg                                      <  2:2170451/100‑1 (MQ=255)
                                                               ctgGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCgg                                     <  1:3609920/100‑1 (MQ=255)
                                                               ctgGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCgg                                     <  2:1674062/100‑1 (MQ=255)
                                                               ctgGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCgg                                     <  1:1907480/100‑1 (MQ=255)
                                                               ctgGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCgg                                     <  1:1810529/100‑1 (MQ=255)
                                                               ctgGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCgg                                     <  1:259206/100‑1 (MQ=255)
                                                                tgGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGt                                    >  1:2703081/1‑100 (MQ=255)
                                                                 ggATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGta                                   <  2:1264803/100‑1 (MQ=255)
                                                                   aTAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGtata                                 <  1:3806629/100‑1 (MQ=255)
                                                                   aTAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGtata                                 >  1:3050978/1‑100 (MQ=255)
                                                                   aTAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGtata                                 <  1:3979769/100‑1 (MQ=255)
                                                                    tAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATAc                                >  1:27873/1‑100 (MQ=255)
                                                                    tAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATAc                                >  1:2076989/1‑100 (MQ=255)
                                                                      aaGATGGGCAGATCATCGACGGTGTGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGCGTGCAGCTCCGGCAGCGGtata                                 >  2:1501844/1‑97 (MQ=255)
                                                                      aaGATGGGCAGATCATCGACGGTGGGAAAGCACTGAGCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACAt                              >  1:2914609/1‑100 (MQ=255)
                                                                        gATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCg                            <  1:2218972/100‑1 (MQ=255)
                                                                          tGGGCAGATCATCGTCGGTGGGAAAGCACTGTCCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAg                          <  2:2818295/100‑1 (MQ=255)
                                                                          tGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAg                          <  1:50059/100‑1 (MQ=255)
                                                                          tGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAg                          <  2:1073099/100‑1 (MQ=255)
                                                                           gggCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGt                         >  1:1164480/1‑100 (MQ=255)
                                                                             gCAGATCATCGACGGTGGGAAAGCACTGACAACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGTTGAGGCTCCGGCAGCGGTATACATCGAGCCt                       >  2:2190817/1‑100 (MQ=255)
                                                                              naGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTg                      >  1:3228292/2‑100 (MQ=255)
                                                                              cAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTg                      <  2:2018849/100‑1 (MQ=255)
                                                                               aGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgc                     >  1:984221/1‑100 (MQ=255)
                                                                                gagcatcGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGGTTTCTCTGTCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcg                    >  2:3715708/4‑100 (MQ=255)
                                                                                gATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcg                    >  2:2973497/1‑100 (MQ=255)
                                                                                    atcGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGcggcgg                <  1:1562280/100‑1 (MQ=255)
                                                                                    atcGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGcggcgg                >  2:4092430/1‑100 (MQ=255)
                                                                                      cGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGt              <  1:1313072/100‑1 (MQ=255)
                                                                                       gaagggggtaaaGCACAGCCCACTGACTTCATGAGCAACGGTGTCTCGCGCCTGGTGGGTGCGCCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTa             <  2:2545650/91‑1 (MQ=255)
                                                                                       gACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTa             >  1:2299734/1‑100 (MQ=255)
                                                                                         cGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcg           <  2:654605/100‑1 (MQ=255)
                                                                                           gTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggc         <  1:2727001/100‑1 (MQ=255)
                                                                                             gggAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcgg       >  2:247653/1‑100 (MQ=255)
                                                                                             gggAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcgg       >  2:2697326/1‑100 (MQ=255)
                                                                                              ggAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcggc      <  1:3158728/100‑1 (MQ=255)
                                                                                               gAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcggcg     >  1:1363990/1‑100 (MQ=255)
                                                                                                 aaGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCggcggg   <  2:1211027/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
GTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGC  >  NC_000913/4279271‑4279468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: