Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 401,440 T→G 54.3% S19A (TCG→GCG)  iraP → anti‑RssB factor, RpoS stabilzer during Pi starvation; anti‑adapter protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913401,4400TG54.3% 71.6 / 205.7 157S19A (TCG→GCG) iraPanti‑RssB factor, RpoS stabilzer during Pi starvation; anti‑adapter protein
Reads supporting (aligned to +/- strand):  ref base T (28/44);  new base G (38/47);  total (66/91)
Fisher's exact test for biased strand distribution p-value = 5.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 158 total aligned reads displayed.

AATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGG  >  NC_000913/401346‑401539
                                                                                              |                                                                                                   
 aTGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAg                                                                                               <  1:973514/100‑1 (MQ=255)
 aTGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAg                                                                                               <  1:41236/100‑1 (MQ=255)
  tGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGa                                                                                              >  2:920877/1‑100 (MQ=255)
    ttAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAAc                                                                                            >  1:1374059/1‑100 (MQ=255)
    ttAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAAc                                                                                            <  2:447610/100‑1 (MQ=255)
    ttAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAAc                                                                                            <  2:177173/100‑1 (MQ=255)
      aaCTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACtg                                                                                          <  2:347274/100‑1 (MQ=255)
      aaCTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACtg                                                                                          <  2:1804734/100‑1 (MQ=255)
       aCTTCTCCATACTTTGGATAAGGAAATACAGACATTAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACtgt                                                                                         <  2:1989606/100‑1 (MQ=255)
        cTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACtgtg                                                                                        >  1:3082055/1‑100 (MQ=255)
        cTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACtgtg                                                                                        <  1:3558603/100‑1 (MQ=255)
        cTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACtgtg                                                                                        <  1:2243517/100‑1 (MQ=255)
           ctcCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTgc                                                                                     <  2:2105330/100‑1 (MQ=255)
             ccATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTgcgc                                                                                   >  1:4017191/1‑100 (MQ=255)
             ccATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTgcgc                                                                                   >  1:216840/1‑100 (MQ=255)
              cATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCa                                                                                  <  1:1410937/100‑1 (MQ=255)
              cATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCa                                                                                  <  1:1475238/100‑1 (MQ=255)
                tACTTTTGATAAGTAGATATAGATATTAAAAATCTCATTGCTGGGTGGTTATTTAAGCGGGGCCAACGAGGAGATGAGGCGAGAGAACTGTGTgcg                                                                                    <  2:3926759/96‑1 (MQ=255)
                tACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAgg                                                                                >  1:3156570/1‑100 (MQ=255)
                tACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAgg                                                                                <  1:1888554/100‑1 (MQ=255)
                 aCTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAAATGTGTGCGCAGGt                                                                               >  1:638314/1‑100 (MQ=255)
                      ggATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAg                                                                          >  2:566585/1‑100 (MQ=255)
                      ggAAAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAg                                                                          <  2:2351575/100‑1 (MQ=255)
                       gATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGc                                                                         <  2:1722369/100‑1 (MQ=255)
                       gATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGc                                                                         <  1:3486967/100‑1 (MQ=255)
                       gATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGc                                                                         >  2:1826577/1‑100 (MQ=255)
                       gATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCACAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGc                                                                         >  2:1815089/1‑100 (MQ=255)
                           aGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTg                                                                     >  1:1295261/1‑100 (MQ=255)
                           aGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTg                                                                     >  1:2345696/1‑100 (MQ=255)
                           aGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTg                                                                     <  1:124444/100‑1 (MQ=255)
                           aGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTg                                                                     <  2:3038780/100‑1 (MQ=255)
                           aGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGCAGAAGCTTTg                                                                     >  2:2611047/1‑100 (MQ=255)
                            ggAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTgg                                                                    <  2:468326/100‑1 (MQ=255)
                            ggAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTgg                                                                    >  2:4051653/1‑100 (MQ=255)
                            ggAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTgg                                                                    >  1:3792467/1‑100 (MQ=255)
                             gAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTCTTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGga                                                                   >  2:2194856/1‑100 (MQ=255)
                             gAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGga                                                                   >  2:2162268/1‑100 (MQ=255)
                             gAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGga                                                                   <  1:1504095/100‑1 (MQ=255)
                             gAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGga                                                                   <  2:1631766/100‑1 (MQ=255)
                              aaaTACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGgag                                                                  <  2:653720/100‑1 (MQ=255)
                              aaaTACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGgag                                                                  <  2:1212298/100‑1 (MQ=255)
                               aaTACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGgaga                                                                 <  1:2913334/100‑1 (MQ=255)
                                aTACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGAt                                                                <  2:112875/100‑1 (MQ=255)
                                aTACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGAt                                                                >  1:1763728/1‑100 (MQ=255)
                                  aCAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTa                                                              <  2:3904502/100‑1 (MQ=255)
                                   cAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTAt                                                             >  1:2012671/1‑100 (MQ=255)
                                     gACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCg                                                           >  2:358465/1‑100 (MQ=255)
                                     gACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCg                                                           <  1:604511/100‑1 (MQ=255)
                                     gACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCg                                                           <  1:1310194/100‑1 (MQ=255)
                                     gACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCg                                                           >  1:2467223/1‑100 (MQ=255)
                                      aCATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGt                                                          <  2:3680522/100‑1 (MQ=255)
                                      aCATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGt                                                          <  1:2100206/100‑1 (MQ=255)
                                       cATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTc                                                         >  1:1235321/1‑100 (MQ=255)
                                        aTGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGCGCGCAGGTAGAAGCTTTGGAGATTATCGTCa                                                        >  1:1567375/1‑100 (MQ=255)
                                          gAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACt                                                      >  2:428266/1‑100 (MQ=255)
                                          gAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACt                                                      <  2:1153469/100‑1 (MQ=255)
                                            aaaaTCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGc                                                    <  1:2526945/100‑1 (MQ=255)
                                                 ctcATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGc                                               <  1:3434421/100‑1 (MQ=255)
                                                   cATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCtt                                             >  1:1391876/1‑100 (MQ=255)
                                                    aTTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAAGTGTGTGCGCAGGTAGAAGCTATCGAGATTATCGCAACCGCAATGc                                               >  2:4019445/1‑97 (MQ=255)
                                                          gAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAata                                      <  2:4011111/100‑1 (MQ=255)
                                                           aGTTTTTATTTAAGCTTGCCCAAAAAGCAGAAGATGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAata                                      <  2:1607059/99‑1 (MQ=255)
                                                            gttgttATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATg                                    >  2:2818776/1‑100 (MQ=255)
                                                            gttgttATTTAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATg                                    >  2:175953/1‑100 (MQ=255)
                                                              tgttATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGgc                                  >  1:3733840/1‑100 (MQ=255)
                                                              tgttATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGgc                                  >  1:3317620/1‑100 (MQ=255)
                                                               gttATTTAAGCTTGCCCAAAAAGAATAAGAGGCTAAAGAACTGTGTGCGCACGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGgct                                 <  2:689519/100‑2 (MQ=255)
                                                                  aTTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCaa                              >  1:3125713/1‑100 (MQ=255)
                                                                     tAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAAt                           >  1:742420/1‑100 (MQ=255)
                                                                     tAAGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAAt                           <  2:2467209/100‑1 (MQ=255)
                                                                       aGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCaaa                             >  2:741251/1‑96 (MQ=255)
                                                                       aGCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGa                         >  1:97959/1‑100 (MQ=255)
                                                                        gCTTGCCCAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGAc                        <  2:3232467/100‑1 (MQ=255)
                                                                          ttGCCCAAAAAGAAGAAAAATAGAACGAACTGTGTTCAAAGTTAGAATTATTGGAGATTATCGTAACTACAATGCTTCGCAATATGGCGTAAAATg                          <  2:821901/96‑1 (MQ=255)
                                                                           tGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCaa                     >  2:2809342/1‑100 (MQ=255)
                                                                             cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACa                   >  2:3371633/1‑100 (MQ=255)
                                                                             cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACa                   >  1:426909/1‑100 (MQ=255)
                                                                             cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACa                   <  1:1813923/100‑1 (MQ=255)
                                                                              ccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAg                  <  1:664479/100‑1 (MQ=255)
                                                                              ccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAg                  <  1:664293/100‑1 (MQ=255)
                                                                              ccAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCACAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAg                  >  2:2474739/1‑100 (MQ=255)
                                                                               cAAAAAGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGc                 >  1:3116021/1‑100 (MQ=255)
                                                                               cAAAAAGAAAAAGAGTCGAAAGAACTGTGTGAGTAGGTAGAAGCTTTTGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAAc                    <  2:734789/97‑1 (MQ=255)
                                                                                 aaaaGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCgg               >  1:3029234/1‑100 (MQ=255)
                                                                                  aaaGAAGAAGAGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGt              <  2:709987/100‑1 (MQ=255)
                                                                                    agaagaagaGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttg            <  1:1083026/100‑1 (MQ=255)
                                                                                    agaagaagaGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttg            <  2:2296339/100‑1 (MQ=255)
                                                                                     gaagaagaGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttga           <  1:2254435/100‑1 (MQ=255)
                                                                                     gaagaagaGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttga           >  1:1045941/1‑100 (MQ=255)
                                                                                     gaagaagaGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttga           <  1:357066/100‑1 (MQ=255)
                                                                                     gaagaagaGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttga           <  2:3025946/100‑1 (MQ=255)
                                                                                      aagaagaGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttgat          <  2:775418/100‑1 (MQ=255)
                                                                                      aagaagaGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttgat          <  2:1453779/100‑1 (MQ=255)
                                                                                      aagaagaGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttgat          <  1:22113/100‑1 (MQ=255)
                                                                                       agaagaGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttgatt         >  1:3828002/1‑100 (MQ=255)
                                                                                       agaagaGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttgatt         <  1:1492783/100‑1 (MQ=255)
                                                                                        gaagaGGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttgattg        >  1:3996734/1‑100 (MQ=255)
                                                                                          agagTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttgattga       >  1:2455692/1‑99 (MQ=255)
                                                                                          agagGCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTtgattgat      >  1:4128180/1‑100 (MQ=255)
                                                                                           gagTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATc     <  2:1345036/100‑1 (MQ=255)
                                                                                              |                                                                                                   
AATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGG  >  NC_000913/401346‑401539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: