Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,296,060 C→T 28.8% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,0600CT28.8% 546.8 / 274.1 379intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base C (148/121);  new base T (42/67);  total (191/188)
Fisher's exact test for biased strand distribution p-value = 4.46e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.18e-02

Only 100 of 386 total aligned reads displayed.

CTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCG  >  NC_000913/4295961‑4296159
                                                                                                   |                                                                                                   
cTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAc                                                                                                     >  2:1314800/1‑100 (MQ=32)
 ttAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGACGGATAAGGCGCTTACGCCGCATCCGACATCAACg                                                                                                    >  1:2054512‑M1/1‑99 (MQ=255)
  tAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACgc                                                                                                   >  1:676423‑M1/1‑98 (MQ=255)
   aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATgcc                                                                                                  <  2:1381043‑M1/100‑4 (MQ=255)
    aCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATgcct                                                                                                 <  2:3089935‑M1/100‑5 (MQ=255)
      gcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCGTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACgcctg                                                                                                <  2:2785473‑M1/99‑6 (MQ=255)
       cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATgcctgat                                                                                              <  1:2565214‑M1/100‑8 (MQ=255)
       cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGATTACACCGCATCCGACATCAACgcctgat                                                                                              >  2:280449‑M1/1‑93 (MQ=255)
         tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGc                                                                                            <  2:1457024/100‑1 (MQ=18)
          cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACgcctgatac                                                                                            >  2:2565199‑M1/1‑90 (MQ=255)
           ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGa                                                                                          <  1:1087914/100‑1 (MQ=18)
            tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGAc                                                                                         >  1:365030/1‑100 (MQ=18)
             aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACg                                                                                        <  1:3518851/100‑1 (MQ=33)
             aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACg                                                                                        <  1:982682/100‑1 (MQ=32)
               cAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCt                                                                                      >  2:1513230/1‑100 (MQ=17)
               cAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt                                                                                      >  1:2747728/1‑100 (MQ=33)
                 ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAGGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTg                                                                                    >  2:3001806/1‑100 (MQ=18)
                 ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt                                                                                      >  2:1088462/1‑98 (MQ=33)
                 ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTg                                                                                    >  2:1772551/1‑100 (MQ=35)
                  gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTg                                                                                    <  1:4104158/99‑1 (MQ=25)
                   ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATgcctgataagacgcggcaa                                                                                  >  1:3393729‑M1/1‑81 (MQ=255)
                   ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGcc                                                                                  >  2:3993397/1‑100 (MQ=38)
                    cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGCCATCAACGCCTGATGCGCCGCTTGCcg                                                                                 >  2:3570968/1‑100 (MQ=255)
                    cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcg                                                                                 >  2:475196/1‑100 (MQ=38)
                    cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcg                                                                                 <  2:3206714/100‑1 (MQ=38)
                      aCGCCAGACAGCGCAATAGCCTGATTTCGCGTGATTGTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcgcg                                                                               <  2:2139833/100‑1 (MQ=255)
                       cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTGGCAGCGt                                                                              >  2:1255603/1‑100 (MQ=255)
                        gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt                                                                              <  2:527313/99‑1 (MQ=38)
                        gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc                                                                             <  1:2968487/100‑1 (MQ=25)
                        gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc                                                                             <  1:3577555/100‑1 (MQ=25)
                         ccAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCt                                                                            >  1:408414/1‑100 (MQ=25)
                          cAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCt                                                                            <  1:2679665/99‑1 (MQ=25)
                          cAGACAGCGCAATAGCCTGATTTAGCGTGATTTGGTAGGTCGGATAATGCGTTTACTCCGCATCCAACATCAATGCCTGATGCGACGCTTGCCGCGTCt                                                                            <  1:1751555/99‑1 (MQ=11)
                           aGACAGCGTAATAGCCTGGTGTCGCGTGAGTTTGTAGGGCGAGTAAGGCGTTTCCGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCt                                                                            <  2:3300145/98‑1 (MQ=11)
                             aCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                        >  2:866488/1‑100 (MQ=12)
                             aCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                        <  1:1645553/100‑1 (MQ=25)
                             aCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                        <  1:880877/100‑1 (MQ=255)
                              cAGCTCAATACCCTGATTTAGCGTGATTTTTTAGGTCGGATAAGGCGTTTACGCCTCATCCTACATCAACGCCTGATGCGACGCTTGCCGCGTCTTa                                                                          <  2:2864644/97‑1 (MQ=14)
                              cAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                       <  1:2354141/100‑1 (MQ=25)
                              cAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                       >  1:3119207/1‑100 (MQ=25)
                               aGCGCAATAGTCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                       <  1:1703615/99‑1 (MQ=255)
                                gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                     >  2:2673560/1‑100 (MQ=17)
                                gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                     >  1:3764971/1‑100 (MQ=255)
                                 cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                    <  2:1969223/100‑1 (MQ=255)
                                   cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACgcctgataagacgcgttaagcgtcgcatcaggcgt                                                                  <  1:184483‑M1/100‑36 (MQ=255)
                                   cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATgcctgataagacgcggcaagcgtcgcatcaggcat                                                                  <  1:1010865‑M1/100‑36 (MQ=255)
                                   cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACgcctgataagacgcggcaagcgtcgcatcaggcgt                                                                  <  1:213805‑M1/100‑36 (MQ=255)
                                    actagcatgatttagcatgattTTGCAGATTGGCTCAGGCGTTTACGCCTCATCCGACATCAACGCTTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                   <  2:3505013/96‑1 (MQ=14)
                                    aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATgcctgataagacgcggcaagcgtcgcatcaggcatt                                                                 <  2:1693403‑M1/100‑37 (MQ=255)
                                        gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctt                                                             >  2:417591/1‑96 (MQ=9)
                                        gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGcc                                                             >  1:488091/1‑100 (MQ=40)
                                        gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGcc                                                             >  1:2836539/1‑100 (MQ=40)
                                         ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGTATCCGACATCAACgcctgataagacgcggcaagcgtcgcatcaggcgttgatgt                                                            <  1:146001‑M1/100‑42 (MQ=255)
                                         ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctta                                                            >  1:548780/1‑95 (MQ=2)
                                         ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGc                                                              >  1:2278900/1‑98 (MQ=255)
                                          cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATgcctgataagacgcggcaagcgtcgcatcaggcattgatgtc                                                           <  2:3780752‑M1/100‑43 (MQ=255)
                                          cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTCCCGCGTCTTATCAGGCCTACGCCAg                                                           >  2:2033386/1‑100 (MQ=25)
                                          cTGATTTAGCGTGATTTTGTAGGACGGATAAGGCGTTTACCCCGCAGCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAg                                                           <  2:57495/100‑1 (MQ=255)
                                             aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACa                                                        <  1:1889066/100‑1 (MQ=40)
                                              tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatcttaaccg                                                        >  2:1895644/1‑90 (MQ=255)
                                              tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAg                                                       <  1:1612070/100‑1 (MQ=255)
                                               ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgc                                                      <  1:1025166/100‑1 (MQ=255)
                                               ttAGCGTGATCTTGTAGGTCTGACGAGGCGTTTACGCCGCACCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgc                                                      <  2:3426880/100‑1 (MQ=14)
                                                 aGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCCACGCCTGAGTCGTGGCTTGACGCATCTTCTTAGCCCTAAGCCCGACAgcgc                                                    >  2:3939168/1‑100 (MQ=11)
                                                  gCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCa                                                   >  1:3904184/1‑100 (MQ=255)
                                                    gTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAAt                                                 >  1:1518910/1‑100 (MQ=255)
                                                    gTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAAt                                                 >  1:2414102/1‑100 (MQ=255)
                                                    gTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAAt                                                 >  1:4117712/1‑100 (MQ=255)
                                                    gTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAAt                                                 <  2:1825911/100‑1 (MQ=40)
                                                    gTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAAt                                                 <  2:22479/100‑1 (MQ=255)
                                                         tttGTGGGGCGGGTAAGGCGTTTCCGCCGCGTCCGGCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGcc                                             <  2:3809198/99‑1 (MQ=14)
                                                         tttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTCCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCt                                            >  2:3711127/1‑100 (MQ=38)
                                                           tGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGa                                          >  2:2490319/1‑100 (MQ=255)
                                                            gTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAt                                         >  1:1346599/1‑100 (MQ=40)
                                                            gTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAt                                         <  1:1966989/100‑1 (MQ=255)
                                                              atgTCGGATGCGGCGTAAACGCCTTATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAttt                                       >  1:2179938/3‑100 (MQ=14)
                                                               tgTCGGATGCGGCGTAAACGCCTTATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTTCGCCAGACAGCGCCAt                                                 >  2:1989895/2‑89 (MQ=14)
                                                               ggTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTa                                      >  1:754579/1‑100 (MQ=255)
                                                                gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCTAGCCGGCGCAATAGCCTGATTTAg                                     >  2:2449969/1‑100 (MQ=255)
                                                                gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAt                                         >  2:3475048/1‑96 (MQ=40)
                                                                  cGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCg                                   <  2:1174385/100‑1 (MQ=40)
                                                                  cGGATAAGGCGTTTACGCCGCATCCGACATAAACGCCTGATGAGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCCATAGCATGATTTAg                                     >  2:2689282/1‑98 (MQ=14)
                                                                  cGGATAAAGCGTTTCGACCGCACACGCTATCAACGCCCGACGCGACGCTGGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGc                                    <  2:2353297/99‑1 (MQ=11)
                                                                       aaGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAtt                              <  1:2027927/100‑1 (MQ=255)
                                                                          gCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTg                           >  1:1244080/1‑100 (MQ=255)
                                                                          gCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTg                           <  1:3188957/100‑1 (MQ=255)
                                                                           cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt                          >  2:3636288/1‑100 (MQ=255)
                                                                                 cgccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCg                    >  1:2525483/1‑100 (MQ=40)
                                                                                   ccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCGGCGCAATAGCCTGCTTTAGCGTCATTTTGTAGGTCGGa                  >  2:68883/1‑100 (MQ=14)
                                                                                   ccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCCCAGACAGCGCTTTATCATTCTTCAGCGTGACTTTTTATGTCgg                   >  2:1659730/1‑99 (MQ=11)
                                                                                    cgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGTGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAt                 <  1:3739662/100‑1 (MQ=255)
                                                                                      cATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAGTTAGCGTGATTTTGTAGGTCGGATaa               >  2:221254/1‑100 (MQ=255)
                                                                                         ccGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGc            >  2:1049410/1‑100 (MQ=38)
                                                                                            aCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGtt         <  1:1388656/100‑1 (MQ=38)
                                                                                              aTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGttt        <  1:399301/99‑1 (MQ=35)
                                                                                              aTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTa       <  1:2097336/100‑1 (MQ=35)
                                                                                               tCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAt      >  1:3428687/1‑100 (MQ=35)
                                                                                               tCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAt      >  1:1178611/1‑100 (MQ=35)
                                                                                                   cGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCg  >  1:650088/1‑100 (MQ=11)
                                                                                                   cGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCg  <  2:771619/100‑1 (MQ=11)
                                                                                                   |                                                                                                   
CTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCG  >  NC_000913/4295961‑4296159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: