Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,340,287 G→A 25.6% L79F (CTT→TTT)  adiA ← arginine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,340,2870GA25.6% 333.1 / 102.2 186L79F (CTT→TTT) adiAarginine decarboxylase
Reads supporting (aligned to +/- strand):  ref base G (54/83);  new base A (20/28);  total (74/112)
Fisher's exact test for biased strand distribution p-value = 8.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

TTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGC  >  NC_000913/4340189‑4340386
                                                                                                  |                                                                                                   
  cATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCCCGTTTTGTTGGCGCTCATGAAGCtt                                                                                                  >  2:958770/1‑100 (MQ=255)
   aTCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTa                                                                                                 >  2:1664548/1‑100 (MQ=255)
    tCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTAc                                                                                                <  2:3908600/100‑1 (MQ=255)
     cGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTAcc                                                                                               <  1:3208115/100‑1 (MQ=255)
      gACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCg                                                                                              >  2:2052864/1‑100 (MQ=255)
        cAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGAt                                                                                            <  2:3350203/100‑1 (MQ=255)
        cAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGAt                                                                                            <  2:3279842/100‑1 (MQ=255)
        cAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGAt                                                                                            >  1:1904975/1‑100 (MQ=255)
          aGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCa                                                                                          >  1:2014890/1‑100 (MQ=255)
           gCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGCTTTGTTGGGGCTCATGAAGCTTACCGATCaa                                                                                         >  2:2790437/1‑100 (MQ=255)
             tCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAAtt                                                                                       <  1:9634/100‑1 (MQ=255)
                agcagGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTc                                                                                    <  2:3178580/100‑1 (MQ=255)
                 gcagGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCt                                                                                   <  2:1612855/100‑1 (MQ=255)
                 gcagGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCt                                                                                   <  2:402195/100‑1 (MQ=255)
                 gcagGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGAGCAATTGTCt                                                                                   >  1:2325383/1‑100 (MQ=255)
                  cagGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTg                                                                                  <  1:58465/100‑1 (MQ=255)
                   agGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGa                                                                                 <  1:1208547/100‑1 (MQ=255)
                   agGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGa                                                                                 <  2:1156702/100‑1 (MQ=255)
                     gTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACg                                                                               >  1:1334710/1‑100 (MQ=255)
                          gATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTg                                                                          >  1:2207704/1‑100 (MQ=255)
                          gATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTg                                                                          <  1:777807/100‑1 (MQ=255)
                           aTCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGa                                                                         <  2:2260435/100‑1 (MQ=255)
                           aTCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGa                                                                         <  2:1628686/100‑1 (MQ=255)
                           aTCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGa                                                                         <  1:1504537/100‑1 (MQ=255)
                             ccATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATg                                                                       <  2:2456966/100‑1 (MQ=255)
                              cATGGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGAt                                                                        <  2:3603675/98‑1 (MQ=255)
                               aTTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGtt                                                                     <  1:1088312/100‑1 (MQ=255)
                               aTTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGtt                                                                     <  1:1724461/100‑1 (MQ=255)
                               aTTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGtt                                                                     >  1:2178744/1‑100 (MQ=255)
                                 tGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCg                                                                   <  1:174819/100‑1 (MQ=255)
                                 tGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCg                                                                   <  1:3718784/100‑1 (MQ=255)
                                     gcgAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTcc                                                                <  2:910417/99‑1 (MQ=255)
                                       gAGGGCTTTTTCCCGATCGCCCAACAGGAAGAGCGGCACATTATGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGa                                                             <  2:2900013/100‑1 (MQ=255)
                                       gAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGa                                                             <  2:1456083/100‑1 (MQ=255)
                                        aGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGAt                                                            <  1:319482/100‑1 (MQ=255)
                                         gggCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATg                                                           >  1:2132007/1‑100 (MQ=255)
                                           gCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGtt                                                         <  2:2983186/100‑1 (MQ=255)
                                           gCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGtt                                                         >  2:3199224/1‑100 (MQ=255)
                                            cTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTc                                                        >  1:2579314/1‑100 (MQ=255)
                                            cTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACCTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTc                                                        >  1:1348275/1‑100 (MQ=255)
                                              ttttCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCa                                                      >  2:777188/1‑100 (MQ=255)
                                                ttCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAtt                                                    >  1:2471912/1‑100 (MQ=255)
                                                 tCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAttt                                                   <  1:2392560/100‑1 (MQ=255)
                                                  cccGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTg                                                  <  2:2608113/100‑1 (MQ=255)
                                                  cccGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTg                                                  >  1:3256637/1‑100 (MQ=255)
                                                      aTCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAg                                              <  1:3466406/100‑1 (MQ=255)
                                                       tCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGc                                             <  1:1956253/100‑1 (MQ=255)
                                                       tCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGc                                             <  1:1830231/100‑1 (MQ=255)
                                                        cGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCt                                            <  1:3999275/100‑1 (MQ=255)
                                                         gCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTg                                           >  1:2106059/1‑100 (MQ=255)
                                                          cccAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGa                                          >  2:3495580/1‑100 (MQ=255)
                                                          cccAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGa                                          >  2:1186664/1‑100 (MQ=255)
                                                           ccAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGaa                                         <  2:2425259/100‑1 (MQ=255)
                                                           ccAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTACATTTGATAGCTGaa                                         >  2:2890620/1‑100 (MQ=255)
                                                            cAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGCCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGa                                          >  1:1165609/1‑98 (MQ=255)
                                                            cAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAAc                                        <  2:3029609/100‑1 (MQ=255)
                                                            cAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAAc                                        <  2:2868408/100‑1 (MQ=255)
                                                               cAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATc                                     <  2:1187994/100‑1 (MQ=255)
                                                               cAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATc                                     <  2:1670353/100‑1 (MQ=255)
                                                                 ggAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAg                                   >  1:3942680/1‑100 (MQ=255)
                                                                 ggAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAg                                   >  1:3561196/1‑100 (MQ=255)
                                                                 ggAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAg                                   >  1:676420/1‑100 (MQ=255)
                                                                   aaGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGc                                 <  1:3031840/100‑1 (MQ=255)
                                                                   aaGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGc                                 <  2:1885427/100‑1 (MQ=255)
                                                                   aaGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGc                                 <  2:3370454/100‑1 (MQ=255)
                                                                    aGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCa                                >  1:3313638/1‑100 (MQ=255)
                                                                     gACCGGCACGTTTTGTTGGCGCTGATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCTATTTGATAGCTGAACATCAGGCAg                               >  2:927434/1‑100 (MQ=255)
                                                                     gACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAg                               >  1:904559/1‑100 (MQ=255)
                                                                     gACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAg                               <  1:2694549/100‑1 (MQ=255)
                                                                       ccGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCCATTGTCTGACGTTTTGATGTTCGTCCGGCTGTTCCATTTGATAGCTGAACATCAGGCAGt                              >  2:732288/1‑99 (MQ=255)
                                                                        cGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCa                            <  1:3495931/100‑1 (MQ=255)
                                                                         ggCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCaa                           >  2:1517800/1‑100 (MQ=255)
                                                                         ggCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCaa                           >  1:1797086/1‑100 (MQ=255)
                                                                         ggCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCaa                           >  2:532788/1‑100 (MQ=255)
                                                                           cACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATg                         <  2:3684682/100‑1 (MQ=255)
                                                                           cACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATg                         <  1:999391/100‑1 (MQ=255)
                                                                           cACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATg                         <  2:4067379/100‑1 (MQ=255)
                                                                           cACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCGGGCGGTCAATg                         <  2:2161319/100‑1 (MQ=255)
                                                                             cGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGc                       <  1:2487011/100‑1 (MQ=255)
                                                                                 ttgttgGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCg                   <  1:1083100/100‑1 (MQ=255)
                                                                                  tgttgGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGt                  >  2:33812/1‑100 (MQ=255)
                                                                                  tgttgGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGt                  <  2:2080959/100‑1 (MQ=255)
                                                                                   gttgGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGtt                 <  2:479786/100‑1 (MQ=255)
                                                                                    ttgGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGttt                <  1:4084120/100‑1 (MQ=255)
                                                                                      ggCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGa              <  2:29996/100‑1 (MQ=255)
                                                                                       gcgcTCATGAAGCTTGCCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGaa             <  1:4073489/100‑1 (MQ=255)
                                                                                       gcgcTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGaa             >  1:834230/1‑100 (MQ=255)
                                                                                        cgcTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGatg            <  1:2802652/100‑3 (MQ=255)
                                                                                        cgcTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAag            <  2:1740687/100‑1 (MQ=255)
                                                                                        cgatcttGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAag            <  2:2263066/94‑1 (MQ=255)
                                                                                         gcTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAATATCAGGCAGTCAATGGCTTCGTTTGAag            <  2:1382961/99‑1 (MQ=255)
                                                                                         gcTCATGAAGCTTACCGAGCAATAGTCTGACGTTTTGATGTTCGGCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATCGCTTCGTTTg               <  2:3858813/96‑1 (MQ=255)
                                                                                         gcTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTTCGGATGTTCCATTTGATAGCTGAGCATCGGGCAGTCAATGGCTTCGTTTGAaga           >  2:4123161/1‑100 (MQ=255)
                                                                                          cTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAagag          <  1:516103/100‑1 (MQ=255)
                                                                                           tCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAagan          <  2:569706/99‑2 (MQ=255)
                                                                                            cATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAagagag        >  2:1781037/1‑100 (MQ=255)
                                                                                             aTGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTAGTTTGAAgagaga       >  2:1554866/1‑100 (MQ=255)
                                                                                              tGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGaa      >  1:1803891/1‑100 (MQ=255)
                                                                                                aaGCTTACCCATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATg    <  1:1635533/100‑1 (MQ=255)
                                                                                                 aGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATgg   <  1:3140823/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
TTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGC  >  NC_000913/4340189‑4340386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑