Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,376,471 A→G 19.5% intergenic (+29/‑82) ecnA → / → ecnB entericidin A membrane lipoprotein, antidote entericidin B/entericidin B membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,376,4710AG19.5% 388.5 / 92.6 204intergenic (+29/‑82)ecnA/ecnBentericidin A membrane lipoprotein, antidote entericidin B/entericidin B membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (74/88);  new base G (19/20);  total (94/110)
Fisher's exact test for biased strand distribution p-value = 8.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.04e-01

Only 100 of 207 total aligned reads displayed.

GTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATT  >  NC_000913/4376372‑4376570
                                                                                                   |                                                                                                   
gTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGa                                                                                                     >  1:2853783/1‑100 (MQ=255)
 tAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGAt                                                                                                    >  1:986380/1‑100 (MQ=255)
   acacCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTc                                                                                                  >  2:1495079/1‑100 (MQ=255)
    cacCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTcc                                                                                                 >  2:1836487/1‑100 (MQ=255)
      ccGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTATTCTCTTACGAAAAATCATCAGGTTcc                                                                                                 >  2:953727/1‑98 (MQ=255)
               gTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCAttttt                                                                                       >  1:1916536/1‑99 (MQ=255)
               gTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTg                                                                                      <  1:3062605/100‑1 (MQ=255)
               gTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTg                                                                                      <  1:367877/100‑1 (MQ=255)
               gTTTCGGCGAAGACATCAAACATCTCGGCAACTACATCTCTCGCGCTGCCAGCTAATTTTTATAATAAAAAAAAAAATCATCAGGTTCCCATCAtttt                                                                                        <  2:1802708/98‑1 (MQ=255)
                 ttCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGc                                                                                    <  1:1112108/100‑1 (MQ=255)
                  tCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGGTTCCCATCATTTTTGGCg                                                                                   <  1:403611/100‑1 (MQ=255)
                   cGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGa                                                                                  >  2:3018421/1‑100 (MQ=255)
                    ggCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGGTTCCCATCATTTTTGGcaga                                                                                 >  2:274912/1‑97 (MQ=255)
                    ggCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGAt                                                                                 >  1:3081377/1‑100 (MQ=255)
                     gCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAACATCATCAGATTCCCATCATTTTTGGCGAtg                                                                                >  1:546229/1‑100 (MQ=255)
                       gAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTATAACAAAAAATCATCAGATTCCCATCATTTTTGGCGAtgtt                                                                              <  2:1098002/100‑1 (MQ=255)
                        aaGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGAtgttg                                                                             <  1:3851805/100‑1 (MQ=255)
                          gACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTc                                                                           >  1:3367360/1‑100 (MQ=255)
                           aCATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCt                                                                          >  1:2193917/1‑100 (MQ=255)
                            cATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCta                                                                         >  1:2021579/1‑100 (MQ=255)
                               cAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGGTTCCCATCATTTTTGGCGATGTTGTCtatta                                                                      >  2:555400/1‑100 (MQ=255)
                               cAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCtatta                                                                      <  2:748561/100‑1 (MQ=255)
                               cAAACATCTCGGCAACTCCATCTCGCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAACATCATCAGATTCCCATCATTTTTGGCGATGTTGTCtatta                                                                      <  2:3567985/100‑1 (MQ=255)
                                 aaCATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATTAGATTCCCATCATTTTTGGCGATGTTGTCTattatt                                                                    <  1:2916004/100‑1 (MQ=255)
                                 aaCATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTattatt                                                                    >  2:2148615/1‑100 (MQ=255)
                                   cATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTaa                                                                  >  1:2374722/1‑100 (MQ=255)
                                    aTCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGGTTCCCATCATTTTTGGCGATGTTGTCTATTATTAAt                                                                 <  1:591852/100‑1 (MQ=255)
                                     tctcGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAAtt                                                                <  2:1671734/100‑1 (MQ=255)
                                     tctcGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTACAAAAAAAAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAAtt                                                                <  2:3936970/100‑1 (MQ=255)
                                        cGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAAtttcc                                                             >  2:2011259/1‑98 (MQ=255)
                                        cGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGc                                                             <  2:3682269/100‑1 (MQ=255)
                                        cGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGc                                                             <  1:1851467/100‑1 (MQ=255)
                                         ggCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCt                                                            <  1:1593377/100‑1 (MQ=255)
                                         ggCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCt                                                            >  2:833129/1‑100 (MQ=255)
                                          gCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCCTCATTTTTGGCGCTTTTGTCTATTATTAATTTGCta                                                           >  2:513000/1‑100 (MQ=255)
                                          gCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCta                                                           >  1:2585565/1‑100 (MQ=255)
                                          gCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCAAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCta                                                           <  2:1591009/100‑1 (MQ=255)
                                           cAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGGTTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCtat                                                          >  1:3644984/1‑100 (MQ=255)
                                           cAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCtat                                                          <  1:2475940/100‑1 (MQ=255)
                                           cAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCtat                                                          >  2:585411/1‑100 (MQ=255)
                                              cTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAgg                                                       <  2:472173/100‑1 (MQ=255)
                                              cTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAgg                                                       <  1:952505/100‑1 (MQ=255)
                                                  aTCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTTTCTTTGTTGTCTATTATTAATTTGCTATAGGCaa                                                    >  2:686770/1‑99 (MQ=255)
                                                   tctctcGCGCTGTCAGCTAATTTTCATTTTCTTCCGCAAAATCATTCGTTTCTCATCTTTTTTTTCGATGTTGTCTATTATTAATTTGCTATAGGCaaa                                                   <  2:3443559/99‑1 (MQ=255)
                                                   tctctcGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCaac                                                   <  2:1770968/99‑2 (MQ=255)
                                                   tctctcGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAAc                                                  <  2:3807638/100‑1 (MQ=255)
                                                    ctctcGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACa                                                 <  2:97077/100‑1 (MQ=255)
                                                    ctctcGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACa                                                 <  2:3749877/100‑1 (MQ=255)
                                                      ctcGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACata                                               >  1:1390733/1‑100 (MQ=255)
                                                      ctcGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACata                                               <  1:3200337/100‑1 (MQ=255)
                                                        cgcgCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTTTTGATGTTGTCTATTATTAATTTGCTATAGGCAAACataa                                              >  2:3647853/1‑99 (MQ=255)
                                                          cgccgcCAGCTAATTAATAATCTCAACAAAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACata                                               <  2:189313/96‑1 (MQ=255)
                                                          cgcTGCCAGCTAATTTTTCTTTCCTTCCGAATAATCACCAGATTCCCCTCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACataaata                                           <  2:2793721/100‑1 (MQ=255)
                                                          cgcTACCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACataaata                                           <  2:1324588/100‑1 (MQ=255)
                                                           gcTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACataaataa                                          <  1:3827719/100‑1 (MQ=255)
                                                           gcTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACataaataa                                          <  1:2118186/100‑1 (MQ=255)
                                                            cTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGGTTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAAc                                         <  2:1642333/100‑1 (MQ=255)
                                                            cTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGGTTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAAc                                         <  1:3212966/100‑1 (MQ=255)
                                                            cTGCCAGCTAATTTTTCTTCTCTTCCAAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACataaataa                                          <  2:3212322/99‑1 (MQ=255)
                                                             tGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACa                                        <  2:2862421/100‑1 (MQ=255)
                                                             tGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACa                                        >  2:467851/1‑100 (MQ=255)
                                                                 aGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTAc                                    <  1:2734464/100‑1 (MQ=255)
                                                                 aGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTAc                                    <  1:1777939/100‑1 (MQ=255)
                                                                  gCTAATTTTTCTTCTCTTCCGAAAAATCATCAGGTTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTAc                                    >  1:1524667/1‑99 (MQ=255)
                                                                  gCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTCGCGATGTTGTCTATTATTAATTTGCTATAGGCACACATAAATAACATTAcc                                   >  2:1939355/1‑100 (MQ=255)
                                                                    tAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTa                                 <  1:686173/100‑1 (MQ=255)
                                                                      aTTTTTCTTCTCTTCCGAAAAATCATCAGGTTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTaaa                               <  1:606135/100‑1 (MQ=255)
                                                                        ttttCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAg                             <  2:1405417/100‑1 (MQ=255)
                                                                        ttttCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAg                             <  1:3620249/100‑1 (MQ=255)
                                                                        ttttCTTCTCTGCCGAGAAATCATCATAGTCCCATCATTTTTGGCGATGTTGTCGATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAg                             <  2:3048761/100‑1 (MQ=255)
                                                                         tttCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAgg                            <  1:2302740/100‑1 (MQ=255)
                                                                            cttctcttcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATACACTTACCTAAAAGGaa                          >  2:2412465/1‑99 (MQ=255)
                                                                            cttctcttcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAg                         <  1:170563/100‑1 (MQ=255)
                                                                            cttctcttcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAg                         <  1:4004657/100‑1 (MQ=255)
                                                                             ttctcttcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGa                        >  1:1197303/1‑100 (MQ=255)
                                                                              tctcttcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGAc                       >  2:249449/1‑100 (MQ=255)
                                                                              tctcttcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGAc                       >  1:1234977/1‑100 (MQ=255)
                                                                                tcttcCGAAAAATCATCAGGTTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACGTAAAAGGAAGACg                      >  2:1857820/1‑99 (MQ=255)
                                                                                tcttcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGt                     <  2:888880/100‑1 (MQ=255)
                                                                                tcttcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGt                     >  2:1077750/1‑100 (MQ=255)
                                                                                tcttcCGAAAAATCATCAGATTCCCATCATTTTTCGCGATGTTGTCTATTATTAATTTGATATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGt                     <  1:2167664/100‑1 (MQ=255)
                                                                                 cttcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGtt                    <  1:108643/100‑1 (MQ=255)
                                                                                    ccGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATg                 >  2:2621311/1‑100 (MQ=255)
                                                                                     cGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATgg                <  1:2507069/100‑1 (MQ=255)
                                                                                      gAAAAATCATCAGGTTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGt               >  1:2654998/1‑100 (MQ=255)
                                                                                      gAAAAATCATCAGGTTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGt               <  1:2217944/100‑1 (MQ=255)
                                                                                      gAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGt               >  1:1657002/1‑100 (MQ=255)
                                                                                        aaaaTCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTga             >  1:3524963/1‑100 (MQ=255)
                                                                                        aaaaTCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTg              >  1:975680/1‑99 (MQ=255)
                                                                                          aaTCATCAGGTTCCCATCATTTTTTTTGTTGTTTTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTgaag           >  2:1154147/1‑100 (MQ=255)
                                                                                          aaTCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTgaag           <  1:2505599/100‑1 (MQ=255)
                                                                                           atcattAGGTTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTgaaga          <  2:3225785/100‑1 (MQ=255)
                                                                                           atcatcAGATTCCCGTCATTTTTGGCGATGTTGTCTATTATTAATTGGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTgaag           <  2:2126093/99‑1 (MQ=255)
                                                                                              atcaGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAGTAACATTACCTAAAAGGAAGACGTTATGGTGaagaag        <  2:2210033/99‑1 (MQ=255)
                                                                                              atcaGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAagaaga       >  2:3766299/1‑100 (MQ=255)
                                                                                               tcaGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGAc      <  2:1468620/100‑1 (MQ=255)
                                                                                                 aGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACaa    >  2:2947464/1‑100 (MQ=255)
                                                                                                  gATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAAt   >  1:3639471/1‑100 (MQ=255)
                                                                                                  gATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAAt   <  1:1184859/100‑1 (MQ=255)
                                                                                                   aTTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAAtt  <  1:2271818/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATT  >  NC_000913/4376372‑4376570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: