Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,439,845 T→A 17.6% intergenic (+52/+27) ytfK → / ← ytfL DUF1107 family protein/UPF0053 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,439,8450TA17.6% 515.9 / 97.0 214intergenic (+52/+27)ytfK/ytfLDUF1107 family protein/UPF0053 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (96/80);  new base A (16/22);  total (112/102)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

Only 100 of 218 total aligned reads displayed.

CCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATC  >  NC_000913/4439747‑4439944
                                                                                                  |                                                                                                   
 cGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTc                                                                                                   <  2:681335/100‑1 (MQ=255)
 cGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTc                                                                                                   <  1:4038709/100‑1 (MQ=255)
    aGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAgg                                                                                                >  2:230814/1‑100 (MQ=255)
     gTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGa                                                                                               >  1:1392636/1‑100 (MQ=255)
     gTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGATATGCAGAAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGa                                                                                               <  1:633779/100‑1 (MQ=255)
       tAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGc                                                                                             <  1:2534958/100‑1 (MQ=255)
         aCCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCg                                                                                           <  1:655146/100‑1 (MQ=255)
         aCCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGAATCAGGAGCCg                                                                                           <  1:3557869/100‑1 (MQ=255)
         aCCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGAATCAGGAGCCg                                                                                           <  2:3546519/100‑1 (MQ=255)
          ccGTCAGGTTATGCGTCTGCCAACAGAGATGGCTTAACGAAAGTGCTATGCAGTAAAAAGTGCTATGGAGTAATGAGATGGCTCATCATTAAGGa                                                                                               >  2:3456183/1‑95 (MQ=255)
           cGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGtt                                                                                         <  2:1851470/100‑1 (MQ=255)
           cGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGAATCAGGAGCCg                                                                                           <  2:1319050/98‑1 (MQ=255)
           cGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGAATCAGGAGCCGtc                                                                                         <  2:2948146/100‑2 (MQ=255)
           cGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGAATCAGGAGCCGtc                                                                                         <  1:2554789/100‑2 (MQ=255)
           cGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGAATCAGGAGCCGtc                                                                                         <  2:3814511/100‑2 (MQ=255)
           cGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGAATCAGGAGCCGt                                                                                          <  1:2045805/99‑1 (MQ=255)
            gTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGtt                                                                                         >  1:597944/1‑99 (MQ=255)
            gTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGAATCAGGAGCCGtct                                                                                        <  1:1128416/100‑3 (MQ=255)
                ggTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGt                                                                                    >  2:2154138/1‑100 (MQ=255)
                  ttATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGttt                                                                                  >  2:165897/1‑100 (MQ=255)
                  ttATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGAATCAGGAGCCGtcttattac                                                                                  <  1:3779850/100‑9 (MQ=38)
                   tATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTc                                                                                 >  1:2436560/1‑100 (MQ=255)
                     tGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTg                                                                               <  1:3960110/100‑1 (MQ=255)
                     tGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTg                                                                               >  2:1898222/1‑100 (MQ=255)
                      gCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTgg                                                                              <  2:1204079/100‑1 (MQ=255)
                       cGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTggg                                                                             >  2:691857/1‑100 (MQ=255)
                         tCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTggggg                                                                           <  2:2741408/100‑1 (MQ=255)
                         tCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTggggg                                                                           >  2:3050263/1‑100 (MQ=255)
                             cAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGCCGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTAc                                                                       >  2:1403859/1‑100 (MQ=255)
                               aaCAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTAcgc                                                                     >  1:1266667/1‑100 (MQ=255)
                               aaCAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTAcgc                                                                     >  1:3205989/1‑100 (MQ=255)
                                 cAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGa                                                                   >  1:1525/1‑100 (MQ=255)
                                 cAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGAATCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGa                                                                   >  1:1821093/1‑100 (MQ=255)
                                  agagATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGAc                                                                  <  1:2392741/100‑1 (MQ=255)
                                     gaTGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCt                                                               >  2:2023780/1‑100 (MQ=255)
                                         gCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTttct                                                           >  1:1611787/1‑100 (MQ=255)
                                          cTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTttctt                                                          >  1:826308/1‑100 (MQ=255)
                                           ttAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTttcttc                                                         <  1:624111/100‑1 (MQ=255)
                                           ttAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTttcttc                                                         <  1:2929450/100‑1 (MQ=255)
                                            tAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTtcttct                                                        <  2:208032/100‑1 (MQ=255)
                                             aaCCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTcttctt                                                       <  1:2078373/100‑1 (MQ=255)
                                             aaCCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTcttctt                                                       <  2:615916/100‑1 (MQ=255)
                                              aCCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTtcttt                                                      <  2:1513095/100‑1 (MQ=255)
                                               ccAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTtcttta                                                     >  1:3182226/1‑100 (MQ=255)
                                                  aagtgctatgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTtctttatct                                                  <  1:1761464/100‑1 (MQ=255)
                                                    gtgctatgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTtctttatcttt                                                <  1:2194037/100‑1 (MQ=255)
                                                     tgctatgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTtctttatcttta                                               >  1:2148253/1‑100 (MQ=255)
                                                         atgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGt                                           <  1:3705582/100‑1 (MQ=255)
                                                           gcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCa                                         >  1:3766328/1‑100 (MQ=255)
                                                            tccgatctagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAg                                        <  2:3772287/92‑1 (MQ=255)
                                                              gtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGCGCCGTTGATGTTACTGGGGGTGACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGc                                      >  2:337851/1‑100 (MQ=255)
                                                              gtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGc                                      <  1:386191/100‑1 (MQ=255)
                                                              gtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTAAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTGAGCGTCa                                         >  2:4037324/1‑97 (MQ=255)
                                                                aaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAg                                    >  1:53133/1‑100 (MQ=255)
                                                                  aaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGtt                                  >  1:1460445/1‑100 (MQ=255)
                                                                    agtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGtttt                                >  1:3321409/1‑100 (MQ=255)
                                                                     gtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTg                               >  1:489628/1‑100 (MQ=255)
                                                                      tgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTgg                              <  2:3273379/100‑1 (MQ=255)
                                                                      tgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTgg                              >  1:2104848/1‑100 (MQ=255)
                                                                         tatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGa                           <  1:1052989/100‑1 (MQ=255)
                                                                          atgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGaa                          >  1:4107790/1‑100 (MQ=255)
                                                                           tgcagtaaTGAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGaaa                         >  1:3289367/1‑100 (MQ=255)
                                                                           tgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGaaa                         <  2:3938462/100‑1 (MQ=255)
                                                                              agtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAggg                      >  1:121245/1‑100 (MQ=255)
                                                                                taataaGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCg                    <  1:1303446/100‑1 (MQ=255)
                                                                                  catcaaCGGCTCCTGAATCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGt                  >  1:2609215/6‑100 (MQ=255)
                                                                                  caacaaCGCCTCCCGAATCAGGAGCCGTTGATGATCCTACGACTCGCGCCACGCGCTCTTCTTGACCTTTAGCGTTGGGCACTTTTGGCg                            >  2:2690443/6‑90 (MQ=255)
                                                                                  ataaGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGCCGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGTGCGGt                  >  2:3567182/1‑100 (MQ=255)
                                                                                   taaGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTg                 <  1:265915/100‑1 (MQ=255)
                                                                                   atcaaCGGCTCCTGAATCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTg                 >  1:573119/5‑100 (MQ=255)
                                                                                    aaGACGGCTCCTGAATCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTgg                <  2:1821080/100‑1 (MQ=255)
                                                                                     caaCGGCTCCTGAATCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGc               >  2:2399193/3‑100 (MQ=255)
                                                                                     aGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGc               >  1:2166019/1‑100 (MQ=255)
                                                                                      aaCGGCTCCTGAATCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGcc              >  2:2208963/2‑100 (MQ=255)
                                                                                       aCGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCt             <  2:1694562/100‑1 (MQ=255)
                                                                                       aCGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCt             <  1:2410862/100‑1 (MQ=255)
                                                                                       aCGGCTCCTGAATCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCt             >  1:1206925/1‑100 (MQ=255)
                                                                                         ggCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTg           >  1:2315322/1‑100 (MQ=255)
                                                                                         ggCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTCATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTg           <  1:975762/100‑1 (MQ=255)
                                                                                         ggCTCCTGAATCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTg           >  1:3620203/1‑100 (MQ=255)
                                                                                          gCTCCTGATTCAGGAGCCGTTGATTTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGc          <  2:531834/100‑1 (MQ=255)
                                                                                          gCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGc          <  2:4075851/100‑1 (MQ=255)
                                                                                          gCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGc          >  1:2986103/1‑100 (MQ=255)
                                                                                          gCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGc          <  1:434132/100‑1 (MQ=255)
                                                                                          gCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCGTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGc          >  2:462860/1‑100 (MQ=255)
                                                                                            tCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCTACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTg        <  2:3635669/100‑1 (MQ=255)
                                                                                            tCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTg        >  1:2714091/1‑100 (MQ=255)
                                                                                            tCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTg        <  2:4001126/100‑1 (MQ=255)
                                                                                            tCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTg        <  2:2515383/100‑1 (MQ=255)
                                                                                             ccTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGt       >  1:3237044/1‑100 (MQ=255)
                                                                                             ccTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGt       >  2:2861684/1‑100 (MQ=255)
                                                                                             ccTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGt       >  1:2872091/1‑100 (MQ=255)
                                                                                             ccTGAATCAGGAGCCGTTGATGTTTCTGGGGGATACGCGAAGCTTTCTTCTTTATCTATAGCGTAAGGCAGATTTGGCGAAAGGGCGGTGGCCTTGCTGt       >  2:3079071/1‑100 (MQ=255)
                                                                                              cTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTc      <  1:3018027/100‑1 (MQ=255)
                                                                                              cTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTc      <  1:516094/100‑1 (MQ=255)
                                                                                              cTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTc      <  1:724169/100‑1 (MQ=255)
                                                                                              cTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTc      >  2:264576/1‑100 (MQ=255)
                                                                                              cTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTGGGCGAAAGGGCGGTGGCCTTGCTGTc      <  2:3947605/100‑1 (MQ=255)
                                                                                               tGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCg     <  2:3627135/100‑1 (MQ=255)
                                                                                                  ttCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATc  >  1:2497093/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
CCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATC  >  NC_000913/4439747‑4439944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: