Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,631,700 T→C 100% L323P (CTT→CCT)  slt → lytic murein transglycosylase, soluble

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,631,7000TC98.9% 684.0 / ‑4.0 197L323P (CTT→CCT) sltlytic murein transglycosylase, soluble
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (85/109);  total (86/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 198 total aligned reads displayed.

GGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTT  >  NC_000913/4631602‑4631796
                                                                                                  |                                                                                                
gggCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCtt                                                                                                 >  2:3179632/1‑100 (MQ=255)
 ggCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTg                                                                                                <  1:1226917/100‑1 (MQ=255)
 ggCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTg                                                                                                >  1:796434/1‑100 (MQ=255)
  ggaaCGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTgg                                                                                               <  1:652062/98‑1 (MQ=255)
  gCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTgg                                                                                               >  1:1185664/1‑100 (MQ=255)
  gCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTgg                                                                                               <  2:564225/100‑1 (MQ=255)
      cgatGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCAcc                                                                                           >  2:736786‑M2/5‑100 (MQ=255)
         tGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGc                                                                                        <  1:2913378‑M2/99‑1 (MQ=255)
          gTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCg                                                                                       <  1:3897640/100‑1 (MQ=255)
            cACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGa                                                                                      >  2:3266887/1‑99 (MQ=255)
            cACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAt                                                                                     <  2:2552628/100‑1 (MQ=255)
            cACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATAGCGCCTGGCACCGCCGAt                                                                                     >  2:2026892/1‑100 (MQ=255)
             aCCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAtc                                                                                    >  2:3219221/1‑100 (MQ=255)
              ncgacgaGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAtcg                                                                                   >  1:3311309/2‑100 (MQ=255)
              ccGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAtcg                                                                                   >  1:1354772/1‑100 (MQ=255)
              ccGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAtcg                                                                                   <  1:2269447/100‑1 (MQ=255)
                 acgaGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAtcgtc                                                                                 <  1:1971493/99‑1 (MQ=255)
                   gaGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTcgc                                                                               <  2:1231915/99‑1 (MQ=255)
                        ggCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGcc                                                                           >  2:43119/1‑98 (MQ=255)
                        ggCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTg                                                                         >  1:2060006/1‑100 (MQ=255)
                        ggCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTg                                                                         <  1:518310/100‑1 (MQ=255)
                        ggCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTg                                                                         >  2:3076720/1‑100 (MQ=255)
                          cGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGaa                                                                       >  2:1032189/1‑100 (MQ=255)
                            aaaTGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATa                                                                     >  2:1607917/1‑100 (MQ=255)
                              aTGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGCACGCCGTGTACGAATGGCTCCTGGCACCGGCGATCGTCGCGGCCTGAATAcc                                                                   >  2:197840/1‑100 (MQ=255)
                              aTGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATAcc                                                                   <  1:653219/100‑1 (MQ=255)
                              aTGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATAcc                                                                   <  1:1032195/100‑1 (MQ=255)
                              aTGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATAcc                                                                   <  1:1625195/100‑1 (MQ=255)
                                ggCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctg                                                                 <  1:826230/100‑1 (MQ=255)
                                ggCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctg                                                                 >  2:2934097/1‑100 (MQ=255)
                                ggCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctg                                                                 <  2:2514229/100‑1 (MQ=255)
                                  cgcgATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctggc                                                               >  1:1193884/1‑100 (MQ=255)
                                  cgcgATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctggc                                                               <  2:2885427/100‑1 (MQ=255)
                                  cgcgATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctggc                                                               >  1:2638922/1‑100 (MQ=255)
                                    cgATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctggctg                                                             >  1:2148537/1‑100 (MQ=255)
                                       tGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGgcg                                                          <  1:1810473/100‑1 (MQ=255)
                                        gACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGgcgc                                                         >  1:362557/1‑100 (MQ=255)
                                         aCGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGcgcg                                                        <  2:4104573/100‑1 (MQ=255)
                                         aCGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGcgcg                                                        <  1:444542/100‑1 (MQ=255)
                                            ccATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCt                                                     <  2:1479225/100‑1 (MQ=255)
                                            ccATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCt                                                     <  2:2598626/100‑1 (MQ=255)
                                             cATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTg                                                    <  1:3154305/100‑1 (MQ=255)
                                             cATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTg                                                    <  1:1576085/100‑1 (MQ=255)
                                             cATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTg                                                    <  2:1915434/100‑1 (MQ=255)
                                               ttATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGcc                                                  <  1:3766101/100‑1 (MQ=255)
                                                tATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGcn                                                  <  2:1945887/99‑2 (MQ=255)
                                                 aTGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGCATACCTGGCTGgcgcctatcact                                                 >  2:3876603/1‑91 (MQ=255)
                                                  tGCGCTCGCAATCTACTTCGCTTATTAAACGCCGTGTACGAATGGCGCCTGGCGCCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGcc                                                  <  2:2327151/97‑1 (MQ=255)
                                                   gcgcTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATg                                              >  1:885457/1‑100 (MQ=255)
                                                    cgcTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATgg                                             >  2:3819198/1‑100 (MQ=255)
                                                       tCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAg                                          <  2:1808740/100‑1 (MQ=255)
                                                        cGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGc                                         >  1:1286467/1‑100 (MQ=255)
                                                          cAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCga                                       >  1:2771357/1‑100 (MQ=255)
                                                           aaTCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaa                                      <  1:3379858/100‑1 (MQ=255)
                                                              cTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaaga                                   >  1:735107/1‑100 (MQ=255)
                                                               tACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagag                                  <  1:1406988/100‑1 (MQ=255)
                                                               tACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagag                                  <  2:3985137/100‑1 (MQ=255)
                                                               tACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagag                                  <  1:100109/100‑1 (MQ=255)
                                                                aCTTCGCTTATTGAACGCCGTGTACAAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagaga                                 >  2:704753/1‑100 (MQ=255)
                                                                 cTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagagaa                                >  1:3026693/1‑100 (MQ=255)
                                                                  ttCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagagaaa                               >  1:2708340/1‑100 (MQ=255)
                                                                   tCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGGGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGACGCggaagagaaag                              >  2:2834034/1‑100 (MQ=255)
                                                                   tCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagagaaag                              >  1:2996591/1‑100 (MQ=255)
                                                                    cGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagagaaaga                             <  1:3994914/100‑1 (MQ=255)
                                                                    cGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagacaaaga                             <  1:1934549/100‑1 (MQ=255)
                                                                     gcctATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAGCGTCGCGGCCTGAATACCTGGCTGGCGCGACTGTCTATGGAAGCgaaaga                                   <  2:2343964/90‑1 (MQ=255)
                                                                      cTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATg                           >  1:617680/1‑100 (MQ=255)
                                                                      cTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATg                           >  1:2853541/1‑100 (MQ=255)
                                                                        tATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGaa                         <  2:8710/100‑1 (MQ=255)
                                                                         aTTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGCAAGATGAAt                        >  2:1421559/1‑100 (MQ=255)
                                                                         aTTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAAt                        >  2:3246589/1‑100 (MQ=255)
                                                                         aTTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCATGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAAt                        <  2:1556125/100‑1 (MQ=255)
                                                                          ttGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATg                       <  2:2091703/100‑1 (MQ=255)
                                                                          ttGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATg                       <  2:2146167/100‑1 (MQ=255)
                                                                            gAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGc                     >  2:474100/1‑100 (MQ=255)
                                                                            gAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGc                     >  1:1260913/1‑100 (MQ=255)
                                                                            gAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGc                     >  1:2194834/1‑100 (MQ=255)
                                                                             aaCGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCg                    >  2:2776744/1‑100 (MQ=255)
                                                                              aCGCCGTGTACGAATGGCGCCTGGCACCGGCGATTGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGt                   <  1:4059474/100‑1 (MQ=255)
                                                                               cGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGtt                  <  1:3179651/100‑1 (MQ=255)
                                                                               cGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGtt                  <  1:5762/100‑1 (MQ=255)
                                                                               cGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATAACTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGtt                  <  1:3868011/100‑1 (MQ=255)
                                                                                gCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTa                 <  1:347112/100‑1 (MQ=255)
                                                                                   gtgtACGAATGGCGCCTGGCACCGGCGACCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTg              <  2:1328671/100‑1 (MQ=255)
                                                                                     gtACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGc            >  2:1239610/1‑100 (MQ=255)
                                                                                     atACGAATAACGCCTAGCACAGGCGATCGTCGCGGCATGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAAAGACGTTACTg              >  2:626953/2‑98 (MQ=255)
                                                                                      tACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGTCGTTACTGGCa           <  1:1731525/100‑1 (MQ=255)
                                                                                       aCGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAg          >  2:3393777/1‑100 (MQ=255)
                                                                                         gAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGc        >  1:186703/1‑100 (MQ=255)
                                                                                         gAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGc        >  1:3929306/1‑100 (MQ=255)
                                                                                          aaTGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCg       >  2:1883340/1‑100 (MQ=255)
                                                                                          aaTGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCg       <  1:2443708/100‑1 (MQ=255)
                                                                                          aaTGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCg       <  2:3010458/100‑1 (MQ=255)
                                                                                              gcgcCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGAtt   <  1:3087656/100‑1 (MQ=255)
                                                                                              gcgcCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGAtt   <  1:2419508/100‑1 (MQ=255)
                                                                                              gcgcCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGAtt   <  1:1593277/100‑1 (MQ=255)
                                                                                              gcgcCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGAtt   <  1:1784766/100‑1 (MQ=255)
                                                                                              gcgcCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGACGAATGGCGTTACTGGCAGGCGGAtt   <  2:2469350/100‑1 (MQ=255)
                                                                                               cgcCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGAtt   >  1:2886911/1‑99 (MQ=255)
                                                                                               cgcCTGCCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGAttt  <  1:3722419/100‑1 (MQ=255)
                                                                                                  |                                                                                                
GGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTT  >  NC_000913/4631602‑4631796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: