BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	432,771	T→G	56.3%	R27R (CGA→CGC)	yajI ←	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	432,771	0	T	G	56.3%	84.4 / 206.9	162	R27R (CGA→CGC)	yajI	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base T (31/40); new base G (45/46); total (76/86)
Fisher's exact test for biased strand distribution p-value = 5.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01

Only 100 of 162 total aligned reads displayed.

ATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGT  >  NC_000913/432674‑432869

aTTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCg                                                                                                  <  1:820294/100‑1 (MQ=255)

     gCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGcactt                                                                                             >  2:357649/1‑100 (MQ=255)

     gCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGcactt                                                                                             >  1:966849/1‑100 (MQ=255)

      cATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGcacttc                                                                                            <  2:4119831/100‑1 (MQ=255)

       aTTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGcacttca                                                                                           >  1:627534/1‑100 (MQ=255)

          ctgCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGcacttcactt                                                                                        <  1:2402414/98‑1 (MQ=255)

           aGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTGGCACTTCACttt                                                                                       <  1:2715087/100‑1 (MQ=255)

           aGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACttt                                                                                       >  1:3506390/1‑100 (MQ=255)

             ccTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTtgc                                                                                     <  2:700009/100‑1 (MQ=255)

             ccTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTtgc                                                                                     >  2:857052/1‑100 (MQ=255)

             ccTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTtgc                                                                                     <  1:3111387/100‑1 (MQ=255)

              cTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTtgct                                                                                    >  1:3185776/1‑100 (MQ=255)

              cTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTtgat                                                                                    >  2:2507270/1‑98 (MQ=255)

               tGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTtgctg                                                                                   <  1:1643403/100‑1 (MQ=255)

               tGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTtgctg                                                                                   <  2:2074075/100‑1 (MQ=255)

                gTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTtgctgc                                                                                  <  1:1893650/100‑1 (MQ=255)

                gTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTtgctgc                                                                                  <  1:3999816/100‑1 (MQ=255)

                gTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTtgctgc                                                                                  <  1:1451765/100‑1 (MQ=255)

                gTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTtgctg                                                                                   >  1:562001/1‑99 (MQ=255)

                 ttttGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGca                                                                                 <  1:3749321/100‑1 (MQ=255)

                      cTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAg                                                                            >  1:1455327/1‑100 (MQ=255)

                         aGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGgcg                                                                         <  1:4044152/100‑1 (MQ=255)

                          gTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGgcgc                                                                        <  2:3072942/100‑1 (MQ=255)

                          gTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGgcgc                                                                        <  1:551231/100‑1 (MQ=255)

                           tGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCt                                                                       >  2:3281781/1‑100 (MQ=255)

                            gATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTa                                                                      >  2:1707836/1‑100 (MQ=255)

                              ttttGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAg                                                                    <  1:2774612/100‑1 (MQ=255)

                               tttGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAgg                                                                   <  2:2698168/100‑1 (MQ=255)

                                ttGTCTGGTTCTTGATTGAGTTGTGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGc                                                                  <  2:1392383/100‑1 (MQ=255)

                                   aCTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGa                                                               <  2:459182/100‑1 (MQ=255)

                                    cTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGaa                                                              >  1:1379770/1‑100 (MQ=255)

                                     tGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAAc                                                             <  1:1707848/100‑1 (MQ=255)

                                      gTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACa                                                            >  2:4038521/1‑100 (MQ=255)

                                      gTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACa                                                            >  2:3974061/1‑100 (MQ=255)

                                       tttCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAg                                                           >  1:1286477/1‑100 (MQ=255)

                                       tttCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAg                                                           <  1:679502/100‑1 (MQ=255)

                                       tttCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAg                                                           <  1:418091/100‑1 (MQ=255)

                                       tttCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAg                                                           <  1:4071015/100‑1 (MQ=255)

                                        ttCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAgg                                                          <  1:3237794/100‑1 (MQ=255)

                                         tCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGc                                                         >  2:3644/1‑100 (MQ=255)

                                            tgattgaGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTc                                                      >  2:741567/1‑100 (MQ=255)

                                            tgattgaGTTGCGTCATCTCCTGGTGCAGCGGGCTGTCGCTGTGTTTCATTTGTCGCACTTCACTTTGCTGCACGCAGGCGCTAAGGCTGAACAGGc                                                         <  2:3310985/97‑1 (MQ=255)

                                             gattgaGTTGCGTCATCTCCTGGTTCAGCGTGGTGACGCTGTGTTGCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTcc                                                     <  1:822934/100‑1 (MQ=255)

                                             gattgaGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTcc                                                     >  2:1254673/1‑100 (MQ=255)

                                              attgaGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTccc                                                    >  1:1790586/1‑100 (MQ=255)

                                                tgaGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCcag                                                  >  2:2508646/1‑100 (MQ=255)

                                                 gaGTTGCGTCATCTCCGGGTTCAGCGTGCTGACGCTGGGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTc                                                      <  2:3117461/95‑1 (MQ=255)

                                                   gTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCcagcag                                               <  1:2824417/100‑1 (MQ=255)

                                                    ttGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAgg                                              <  2:3203089/100‑1 (MQ=255)

                                                     tGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGa                                             >  2:106668/1‑100 (MQ=255)

                                                     tGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGa                                             <  2:187472/100‑1 (MQ=255)

                                                      gCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCAATTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAg                                            <  2:1327159/100‑1 (MQ=255)

                                                       cGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGc                                           <  2:556371/100‑1 (MQ=255)

                                                        gTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCa                                          <  2:1552650/100‑1 (MQ=255)

                                                        gTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTCAGGCTGAACAGGCTTACCagcagcagc                                           >  2:3112398/1‑99 (MQ=255)

                                                         tCATCTCCTGGTTCAGCGTGCTGACGCCGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCGAAGGCTGAACAGGCTTCCCAGCAGGGGCa                                          <  1:243650/99‑1 (MQ=255)

                                                           aTCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTc                                       >  2:2879382/1‑100 (MQ=255)

                                                           aTCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTc                                       <  1:3280083/100‑1 (MQ=255)

                                                            tctcCTGGTTAAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCAAACAGGCGCTAAGGCTGAACAGGTTTCCCAGAAGGAGCAGTCg                                      <  2:830362/100‑1 (MQ=255)

                                                              tcCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGaa                                    >  1:2089784/1‑100 (MQ=255)

                                                               ccTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGaaa                                   >  1:869374/1‑100 (MQ=255)

                                                                 tGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGaaaaa                                 >  1:865833/1‑100 (MQ=255)

                                                                 tGGTTCAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGaaaaa                                 >  1:3743028/1‑100 (MQ=255)

                                                                  ggTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAAc                                >  1:1508509/1‑100 (MQ=255)

                                                                      cAGCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTa                            >  1:2303121/1‑100 (MQ=255)

                                                                       aGCGTGGTGACGCTGTGTTTCGTCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAATACGTTAg                           >  2:2593451/1‑100 (MQ=255)

                                                                       aGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAg                           >  2:1637216/1‑100 (MQ=255)

                                                                       aGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAg                           <  1:6192/100‑1 (MQ=255)

                                                                       aGCGTGCGGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCAGACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAg                           <  2:1617189/100‑1 (MQ=255)

                                                                        gCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGt                          >  2:43590/1‑100 (MQ=255)

                                                                        gCGTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGt                          >  2:3351364/1‑100 (MQ=255)

                                                                         tcctatctcGCTGTGTTTCATCTAGCTCACTTCACTTTGCTGCACACAGACGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGt                          <  2:3801104/91‑1 (MQ=255)

                                                                         cGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTa                         >  2:2638998/1‑100 (MQ=255)

                                                                          gTGCTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTAt                        >  2:3694531/1‑100 (MQ=255)

                                                                           tGCTGACGCTATGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCTCTAAGGCTGAACAGGCTTCCCAGCAAAAAAAAAAAAAAAACGTTAGTAtt                       <  2:2232493/100‑1 (MQ=255)

                                                                             cTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCa                     >  2:1376466/1‑100 (MQ=255)

                                                                             cTGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCa                     >  2:2605160/1‑100 (MQ=255)

                                                                              tGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCAt                    >  2:64110/1‑100 (MQ=255)

                                                                              tGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCAt                    >  2:3426328/1‑100 (MQ=255)

                                                                              tGACGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCAt                    <  1:1010425/100‑1 (MQ=255)

                                                                               gACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATa                   >  2:880749/1‑100 (MQ=255)

                                                                                 cGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAgg                 <  1:637241/100‑1 (MQ=255)

                                                                                 cGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAgg                 <  2:2387660/100‑1 (MQ=255)

                                                                                 cGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAgg                 >  1:1507161/1‑100 (MQ=255)

                                                                                 cGCTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGAGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTagatcggaa                 >  1:3801128/1‑92 (MQ=255)

                                                                                  gttgtcgttCATATGGCTCATTTTACTTTTCGGCACACAGGTCCGAAGGCTGAACAGGCTTCCCTGGAGGAGCAGGCGAAAAACGTTGGTATTCATAgg                 <  2:3807617/92‑1 (MQ=255)

                                                                                  gCTGTGTTTCATCTGTCGCACTTCACCTTGCAGAACACAGGCGCTAAGGCTGAACAAGCTTCCCAACAGGAGCAGTCGAAAAACGTTAGTATTCATAGgt                <  2:607868/100‑1 (MQ=255)

                                                                                   cTGTGTTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGgt                >  2:1453587/1‑99 (MQ=255)

                                                                                      tgtTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGgtcgtc            <  1:1094647/100‑1 (MQ=255)

                                                                                       gtTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTcc           >  1:3989210/1‑100 (MQ=255)

                                                                                       gtTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTcc           >  1:3593697/1‑100 (MQ=255)

                                                                                       gtTTCATCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTcc           <  2:978044/100‑1 (MQ=255)

                                                                                         ttCATCTGTCGCACTTCACTTTGCTGGACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCt         <  2:1429287/100‑1 (MQ=255)

                                                                                         ttCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCt         <  2:1601879/100‑1 (MQ=255)

                                                                                         ttCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCt         >  1:3208655/1‑100 (MQ=255)

                                                                                           cATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCttt       >  1:1335647/1‑100 (MQ=255)

                                                                                            aTCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTtc      <  2:3743267/100‑1 (MQ=255)

                                                                                            aTCTGGCGCACTTCACTTTGCTGCACACAGGCGCTACGGCTGAACATGCTTCCCATCAGCAGCGGTCGAAAAACGTGCGTATTCAGATGTCGTcccgtac      >  2:3086694/1‑96 (MQ=255)

                                                                                             tCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTtct     <  1:3671233/100‑1 (MQ=255)

                                                                                             tCTGGCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTtct     <  2:2679812/100‑1 (MQ=255)

ATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGT  >  NC_000913/432674‑432869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`