Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 459,256 T→C 16.7% D123D (GAT→GAC lon → DNA‑binding ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913459,2560TC16.7% 263.7 / 39.0 119D123D (GAT→GAClonDNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base T (45/54);  new base C (10/10);  total (55/64)
Fisher's exact test for biased strand distribution p-value = 8.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

Only 100 of 129 total aligned reads displayed.

GCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACGTCGCTG  >  NC_000913/459157‑459355
                                                                                                   |                                                                                                   
gCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATtgat                                                                                                     >  2:3953995/1‑100 (MQ=255)
  tGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATtgatga                                                                                                   >  1:1024599/1‑100 (MQ=255)
  tGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATtgatga                                                                                                   >  2:717010/1‑100 (MQ=255)
   ggTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAg                                                                                                  >  2:3178645/1‑100 (MQ=255)
   ggTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAg                                                                                                  >  1:321430/1‑100 (MQ=255)
      cGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCgg                                                                                               >  1:1654922/1‑100 (MQ=255)
         ggggTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGaa                                                                                            >  2:2142337/1‑100 (MQ=255)
           ggTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACa                                                                                          <  2:3954010/100‑1 (MQ=255)
           ggTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACa                                                                                          >  1:3622469/1‑100 (MQ=255)
            gTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAg                                                                                         <  2:10303/100‑1 (MQ=255)
            gTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAg                                                                                         <  1:3529941/100‑1 (MQ=255)
                 aGCGCGCGCGTATGTCTGCGCGCTCTGACAATGGCTAACACTTTTCTGCGAAGGCGGAGTATCGGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAg                                                                                     <  2:328501/99‑1 (MQ=255)
                   cgcgcgcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGc                                                                                  <  1:1221133/100‑1 (MQ=255)
                       cgcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGt                                                                              >  2:640560/1‑100 (MQ=255)
                       cgcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTgg                                                                               >  2:638138/1‑99 (MQ=255)
                       cgcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTCATGAGCGGGAACAGGAAGTGcgg                                                                                >  2:2216703/1‑96 (MQ=255)
                        gcgTATTTCTGCGCTCTCTAACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTg                                                                             <  2:96527/100‑1 (MQ=255)
                           tATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGt                                                                          <  1:4060945/100‑1 (MQ=255)
                            atgtCTGCGCTCTCCGACAGTGGCGAAAACTTTAATGCGAAGGCGGAGTATCTGGAGTCATCGACCATTGATGAGCGGGAACAGGAAgtggtggtg                                                                             <  2:1730024/93‑1 (MQ=255)
                            aTTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTa                                                                         >  1:7233/1‑100 (MQ=255)
                            aTTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTa                                                                         >  1:3187622/1‑100 (MQ=255)
                             tttCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTAc                                                                        >  1:1883011/1‑100 (MQ=255)
                              ttCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTAc                                                                        <  2:1348032/99‑1 (MQ=255)
                               tCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTg                                                                      >  1:3259018/1‑100 (MQ=255)
                               tCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTg                                                                      <  1:28647/100‑1 (MQ=255)
                                cTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGc                                                                     <  2:2381469/100‑1 (MQ=255)
                                cTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGc                                                                     <  1:2416708/100‑1 (MQ=255)
                                cTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGc                                                                     >  1:1832004/1‑100 (MQ=255)
                                 tGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGc                                                                     <  2:494142/99‑1 (MQ=255)
                                 tGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCa                                                                    >  2:1698659/1‑100 (MQ=255)
                                 tGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCa                                                                    >  1:3974758/1‑100 (MQ=255)
                                  gcgcTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCaa                                                                   >  1:2988605/1‑100 (MQ=255)
                                  gcgcTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCaa                                                                   >  1:1699917/1‑100 (MQ=255)
                                  gcgcTCTCTGACAATAGCAAACACTTTTCTGCGAAGGCGTAGTATCTGGAGTCGCCGACCATTGATGAGCGTGAACAGGAAGTGCTGGTCCGTACTGCaa                                                                   >  2:1216555/1‑100 (MQ=255)
                                    gcactctGGCAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGGCGCCGCCCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACCGCa                                                                    <  2:673945/94‑1 (MQ=255)
                                    gcTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATc                                                                 <  2:456941/100‑1 (MQ=255)
                                     ctctctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCa                                                                >  2:2921172/1‑100 (MQ=255)
                                     ctctctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCa                                                                >  1:438030/1‑100 (MQ=255)
                                     ctctctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCa                                                                <  1:2783057/100‑1 (MQ=255)
                                     ctctctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCa                                                                <  2:1414163/100‑1 (MQ=255)
                                        tctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGcc                                                             <  1:3654241/100‑1 (MQ=255)
                                         ctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGCACCGGCAGTGCTGGTGCGTACTGCAATCACCCa                                                            >  2:2262264/1‑100 (MQ=255)
                                          tGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAg                                                           <  2:3546868/100‑1 (MQ=255)
                                           gACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAAGAAGAAGAGCTGGTGCGTATTGCAAACAGCGAg                                                           >  2:2862630/1‑99 (MQ=255)
                                           gACAATGGCGAACACTTTTCTGCGAAGGCGCAGGATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGt                                                          >  2:2711438/1‑100 (MQ=255)
                                             cAATGGCGAACACTTTTCTGCGAAGGGGGAGTATCTGGAGGGGCCGACCAGTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGcaa                                                            >  2:3627114/1‑94 (MQ=255)
                                             cAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTc                                                        <  2:4005834/100‑1 (MQ=255)
                                             cAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTc                                                        >  1:2142630/1‑100 (MQ=255)
                                               aTGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGa                                                      >  1:1049818/1‑100 (MQ=255)
                                                tGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGaa                                                     <  1:1962848/100‑1 (MQ=255)
                                                 ggCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAg                                                    >  1:3548126/1‑100 (MQ=255)
                                                 ggCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAg                                                    <  2:377026/100‑1 (MQ=255)
                                                  gCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGGAgg                                                   >  1:3754821/1‑100 (MQ=255)
                                                   cGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGc                                                  >  2:1182296/1‑100 (MQ=255)
                                                    gAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGc                                                  <  2:483373/99‑1 (MQ=255)
                                                         cTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATc                                            >  1:3875211/1‑100 (MQ=255)
                                                         cTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATc                                            >  1:1749793/1‑100 (MQ=255)
                                                              cTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCt                                       <  1:717016/100‑1 (MQ=255)
                                                              cTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCt                                       <  2:533163/100‑1 (MQ=255)
                                                               tGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTg                                      <  1:2781431/100‑1 (MQ=255)
                                                                  gAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTATATCAAGCTGAAc                                   <  1:2711454/100‑1 (MQ=255)
                                                                  gAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAAc                                   <  2:3184111/100‑1 (MQ=255)
                                                                  gAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAAc                                   <  1:3639824/100‑1 (MQ=255)
                                                                     ggCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACaaa                                <  2:1870094/100‑1 (MQ=255)
                                                                      gCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACaaaa                               >  2:2890684/1‑100 (MQ=255)
                                                                          aGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAAt                           <  1:1149682/100‑1 (MQ=255)
                                                                             aTCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATccc                        <  2:3151713/100‑1 (MQ=255)
                                                                             aTCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATccc                        <  1:1008603/100‑1 (MQ=255)
                                                                             aTCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATccc                        <  2:2121764/100‑1 (MQ=255)
                                                                             aTCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATccc                        <  1:2619664/100‑1 (MQ=255)
                                                                             aTCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATccc                        >  2:1562480/1‑100 (MQ=255)
                                                                             aTCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGGGCGTAATGCAATCAGCCAGTTCGAAGGCTACATCAAAAAAAAAAAAAAAATccc                        <  2:3585784/100‑1 (MQ=255)
                                                                              tCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCcca                       >  2:311427/1‑100 (MQ=255)
                                                                              tCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCcca                       <  1:3391981/100‑1 (MQ=255)
                                                                               cTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCccac                      <  1:1484092/100‑1 (MQ=255)
                                                                                tGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCccacc                     >  2:2596944/1‑100 (MQ=255)
                                                                                tGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCccacc                     <  1:3460889/100‑1 (MQ=255)
                                                                                tGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCccacc                     <  1:1226744/100‑1 (MQ=255)
                                                                                tGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCcca                       <  1:503185/98‑1 (MQ=255)
                                                                                  gAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCacacc                     >  1:2711664/1‑98 (MQ=255)
                                                                                   agacGCCGACCATTGATGAGCGGCAACAGGAAGTGCTGGTGCGAAATGCAATCAGCCAGTCCGATGGCTACATAAAAAAAAAAAAAAAAATCccac                      <  2:192353/93‑1 (MQ=255)
                                                                                   aGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGa                  <  1:1074599/100‑1 (MQ=255)
                                                                                     tCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAg                <  1:3934922/100‑1 (MQ=255)
                                                                                       gCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTg              >  1:4013300/1‑100 (MQ=255)
                                                                                         cGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCt            >  2:1046773/1‑100 (MQ=255)
                                                                                         cGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCt            <  1:3352147/100‑1 (MQ=255)
                                                                                          gACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTg           >  2:1824508/1‑100 (MQ=255)
                                                                                           aCCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCACGCTGAACAAAAAAATCCCACCAGAAGTGCTGa          >  1:221826/1‑100 (MQ=255)
                                                                                           aCCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGa          >  2:1591377/1‑100 (MQ=255)
                                                                                            ccATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGAc         <  1:3419072/100‑1 (MQ=255)
                                                                                            ccATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGAc         >  1:1797747/1‑100 (MQ=255)
                                                                                            ccATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGAc         >  2:1470801/1‑100 (MQ=255)
                                                                                            ccATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGAc         >  1:236143/1‑100 (MQ=255)
                                                                                               ttGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACGTc      <  2:2085543/100‑1 (MQ=255)
                                                                                                tgatgaGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACGTCg     <  1:1343733/100‑1 (MQ=255)
                                                                                                 gatgaGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACGTCGc    >  1:345094/1‑100 (MQ=255)
                                                                                                 gatgaGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACGTCGc    >  1:1498649/1‑100 (MQ=255)
                                                                                                  atgaGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACGTCGCt   <  1:1883642/100‑1 (MQ=255)
                                                                                                  atgaGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACGTCGCt   >  2:2470749/1‑100 (MQ=255)
                                                                                                   tgaGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACGTCGCTg  >  2:558261/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
GCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACGTCGCTG  >  NC_000913/459157‑459355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: