Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 555,549 T→C 59.1% R313R (CGT→CGC cysS → cysteinyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913555,5490TC59.1% 100.5 / 162.0 156R313R (CGT→CGCcysScysteinyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (24/39);  new base C (58/33);  total (83/73)
Fisher's exact test for biased strand distribution p-value = 1.93e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 164 total aligned reads displayed.

GAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATG  >  NC_000913/555453‑555648
                                                                                                |                                                                                                   
gAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGcgcgcgcg                                                                                                  >  2:2293121/1‑100 (MQ=255)
 aaaTACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTgcag                                                                                                 >  2:2513944/1‑98 (MQ=255)
    ttcttcgacgCGGAAACCGTGCGTTACTTACGGATGTCGGGCCACTATCGCAGCCAGGTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTgcggcg                                                                                               <  2:1641046/94‑1 (MQ=255)
    tactacGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGGAGAGAACCTGAAGCAGGCGCGTGCGgcgc                                                                                              >  1:516198/1‑100 (MQ=255)
     actacGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGgcgc                                                                                              >  2:738838/1‑99 (MQ=255)
      ctacGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTg                                                                                            <  1:1991396/100‑1 (MQ=255)
        acgacgCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGa                                                                                          >  1:2669869/1‑100 (MQ=255)
        acgacgCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGa                                                                                          >  2:818392/1‑100 (MQ=255)
        acgacgCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGa                                                                                          <  2:887630/100‑1 (MQ=255)
        acgacgCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGa                                                                                          >  1:369679/1‑100 (MQ=255)
         cgacgCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTg                                                                                            >  1:2409556/1‑97 (MQ=255)
           acgCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCg                                                                                       <  2:486684/100‑1 (MQ=255)
            cgcgGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCg                                                                                       <  2:563748/99‑1 (MQ=255)
            cgcgGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGt                                                                                      >  1:1887550/1‑100 (MQ=255)
             gctgAAACCGTGCGTTACTTCCTGATGTCGGGCCACGATCGCAGCCAGTTGAACTACAGCGAAGAGAACCCGAAGCAGGCGCGTGCGGCGCTGGAGCGtc                                                                                     <  2:2660830/97‑1 (MQ=255)
             gcgGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGtc                                                                                     <  2:1730257/100‑1 (MQ=255)
              cgGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGtct                                                                                    >  1:2252376/1‑100 (MQ=255)
               ggAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGtctc                                                                                   >  1:2256650/1‑100 (MQ=255)
               ggAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGtctc                                                                                   >  1:3835360/1‑100 (MQ=255)
                gAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTctct                                                                                  >  2:3155963/1‑100 (MQ=255)
                   aCCGTGCGTTACTTCCTGATGTCGGGCCACTATAGCAGCCAGTTGAACTACAGCGAAGAGACCCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTa                                                                                 >  2:2927556/1‑98 (MQ=255)
                    ccGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTacac                                                                              <  1:1570669/100‑1 (MQ=255)
                    ccGTGCGTTACTTCCTGATGTCGGGCCACTATCACAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCACGCGCGCGCGGCGCTGGAGCGTCTCTacac                                                                              >  2:2487630/1‑100 (MQ=255)
                      gTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGACGCAGGCGCGCGCGGCGCCGGAGCGTCTCTACActg                                                                            >  2:2533992/1‑100 (MQ=255)
                       tGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACActgc                                                                           <  2:3272329/100‑1 (MQ=255)
                         cGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACActgcgc                                                                         <  1:2630157/100‑1 (MQ=255)
                             aCTTCCTGATGTGGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACActgcgctgcg                                                                     >  1:2712750/1‑100 (MQ=255)
                               ttCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGcgcg                                                                   <  1:4009301/100‑1 (MQ=255)
                                tCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCgg                                                                  <  1:3028411/100‑1 (MQ=255)
                                tCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCgg                                                                  <  2:1297696/100‑1 (MQ=255)
                                 ccTGATGTCGGGCCACTATCGCAGCCAGTTGGACTACAGCGAAGAGAACCTGAAGCAGGCGGGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGc                                                                 <  2:3076153/100‑1 (MQ=255)
                                  cTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCggaa                                                                >  1:2131314/1‑98 (MQ=255)
                                  cTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGca                                                                >  1:1575455/1‑100 (MQ=255)
                                   tGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGcac                                                               >  1:3570365/1‑100 (MQ=255)
                                   tGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGcac                                                               <  1:1966961/100‑1 (MQ=255)
                                    gATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGCAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGcaca                                                              >  2:1797411/1‑100 (MQ=255)
                                    gATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGcaca                                                              >  1:2530277/1‑100 (MQ=255)
                                    gATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGcaca                                                              <  1:3109967/100‑1 (MQ=255)
                                       gTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGAt                                                           >  1:820248/1‑100 (MQ=255)
                                         cGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGTAGGCGGGCGCGGCGACGGAGGGGATCTACACTGCGCTGCGCGGCACAGATaa                                                         <  2:34123/100‑1 (MQ=255)
                                         cGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATaa                                                         >  1:3194562/1‑100 (MQ=255)
                                         cGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATaa                                                         >  2:3277953/1‑100 (MQ=255)
                                             ccACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAAcc                                                     <  1:3902039/100‑1 (MQ=255)
                                               aCTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACAGTGCGCTGCGCGGCACAGATAAAACCGt                                                   >  2:3056492/1‑100 (MQ=255)
                                                  aTCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAGAACCGTTgc                                                >  2:2529198/1‑100 (MQ=255)
                                                    cGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTgcgc                                              <  1:914726/100‑1 (MQ=255)
                                                    cGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTgcgc                                              <  1:2347569/100‑1 (MQ=255)
                                                     gCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGcc                                             <  1:4009339/100‑1 (MQ=255)
                                                     gCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGcc                                             >  1:1197196/1‑100 (MQ=255)
                                                      cAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCt                                            <  1:3742613/100‑1 (MQ=255)
                                                        gCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGc                                          >  1:33978/1‑100 (MQ=255)
                                                        gCCAGTTGAACTACAGCGAAGAGAACCTGAAACAGGCGCGCGGGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGc                                          >  1:2792605/1‑100 (MQ=255)
                                                        gCCAGTTGAACTACAGCGAAGAGAACCTAAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGc                                          >  1:998608/1‑100 (MQ=255)
                                                          cAGCTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTCGCGcc                                             >  2:249565/1‑95 (MQ=255)
                                                           aGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCgg                                       >  2:1628872/1‑100 (MQ=255)
                                                             ttGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTg                                     <  2:2935171/100‑1 (MQ=255)
                                                              tGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTgg                                    >  1:1627261/1‑100 (MQ=255)
                                                                  cTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGaa                                >  2:4135367/1‑100 (MQ=255)
                                                                  cTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGaa                                >  2:381581/1‑100 (MQ=255)
                                                                  cTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGaa                                >  1:3373636/1‑100 (MQ=255)
                                                                   tACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAg                               <  2:3460452/100‑1 (MQ=255)
                                                                   tACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAg                               <  1:1493282/100‑1 (MQ=255)
                                                                    aCAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATACAACCGTTGCGCCTGCCGGTGGCGAAGc                              >  2:553956/1‑100 (MQ=255)
                                                                    aCAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGc                              >  1:1278099/1‑100 (MQ=255)
                                                                      aGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAACGTCTCTACACTGCGCGGCGCGGCCCAGGTAAAACCGTTGCGCCTGCCGGTGGCGAAGCg                             >  2:2544428/1‑99 (MQ=255)
                                                                      aGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGt                            >  1:2558184/1‑100 (MQ=255)
                                                                      aGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGt                            >  1:2558185/1‑100 (MQ=255)
                                                                      aGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGt                            >  1:583089/1‑100 (MQ=255)
                                                                       gCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGtt                           >  1:3729968/1‑100 (MQ=255)
                                                                       gCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGAGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGt                            >  2:238841/1‑99 (MQ=255)
                                                                        cGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAACCCGTTGCGCCTGCCGGTGGCGAAGCGttt                          <  1:543779/100‑1 (MQ=255)
                                                                         gAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTg                         >  1:3615080/1‑100 (MQ=255)
                                                                           agagAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGaa                       <  1:2613174/100‑1 (MQ=255)
                                                                            gagaACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAg                      <  2:3742375/100‑1 (MQ=255)
                                                                              gaACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAgcg                    >  2:3867463/1‑100 (MQ=255)
                                                                              gaACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGACTCTACACTGCACTGCGCGGCACAGATAAAACCAGGGCGCGTGCCGGGAGCGAAGAGCTTGAAgcg                    >  2:1362284/1‑100 (MQ=255)
                                                                              gaACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGACGGTGGCGAAGCTTTTGAAg                      >  2:2246180/1‑98 (MQ=255)
                                                                               aaCCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAgcgc                   <  2:1178499/100‑1 (MQ=255)
                                                                                aCCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAgcgcg                  >  2:15079/1‑100 (MQ=255)
                                                                                aCCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACAGTTGCGCCTGCCGGTGGCGCAGCGTTTGAAgcgc                   >  2:207902/1‑99 (MQ=255)
                                                                                 cttGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAgcgcgc                 >  1:2803818/3‑100 (MQ=255)
                                                                                 ccTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAgcgcgc                 >  2:1253829/1‑100 (MQ=255)
                                                                                 ccTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAgcgcgc                 >  1:3789124/1‑100 (MQ=255)
                                                                                  cTGAAGCAGGCTGGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAgcgc                   <  2:237197/97‑1 (MQ=255)
                                                                                    gAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCttt              <  1:68400/100‑1 (MQ=255)
                                                                                     aaGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTa             <  2:3554756/100‑1 (MQ=255)
                                                                                      aGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTCCGCTGCGCGGCACAGATAAACCCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTCAt            >  2:776361/1‑100 (MQ=255)
                                                                                      aGCAGGCGCGCGCGGCGCCGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTAt            <  2:3497834/100‑1 (MQ=255)
                                                                                         aGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTg          <  2:2409542/99‑1 (MQ=255)
                                                                                         aGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCttcaataa         <  2:3479545/100‑7 (MQ=255)
                                                                                          ggCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGaa        >  1:2001332/1‑100 (MQ=255)
                                                                                          ggCGCGCGCGGCGCTGGAGCGTCTCTACGCTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGAGGCGAAGCGTCTGAAGCGCGCTTTATGGa         >  2:2634650/1‑99 (MQ=255)
                                                                                          ggCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGaa        >  1:2921379/1‑100 (MQ=255)
                                                                                          ggCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGaa        >  2:2633283/1‑100 (MQ=255)
                                                                                          ggCGCGCGCGGCGCTCGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGaa        <  1:4080663/100‑1 (MQ=255)
                                                                                          ggCGCGAGCGGCGCTGGAGCGTCTCTACACTGCGCTGCTCGGCACAGATAAAACCGTTGCGCCTGCAGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGa         <  2:1259303/99‑1 (MQ=255)
                                                                                           gcgcgcgcGGCGCTGGAGCGATCCTACACTGCGCTGCGCCGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAg       >  2:1993121/1‑100 (MQ=255)
                                                                                            cgcgcgcgGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGc      <  2:3572993/100‑1 (MQ=255)
                                                                                            cgcgcgcgGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGc      <  1:801860/100‑1 (MQ=255)
                                                                                                tGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGGTTGAAGCGCGCTTTATTGAAGCGATg  >  2:3960225/1‑100 (MQ=255)
                                                                                                |                                                                                                   
GAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATG  >  NC_000913/555453‑555648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: