Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 129,360 G→T 24.8% intergenic (+24/+47) lpd → / ← yacH dihydrolipoyl dehydrogenase; E3 component of pyruvate and 2‑oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase/DUF3300 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913129,3600GT24.8% 346.8 / 120.6 189intergenic (+24/+47)lpd/yacHdihydrolipoyl dehydrogenase; E3 component of pyruvate and 2‑oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase/DUF3300 family protein
Reads supporting (aligned to +/- strand):  ref base G (77/63);  new base T (23/24);  total (102/87)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.79e-01

Only 100 of 190 total aligned reads displayed.

TCTGTGGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACTGCTG  >  NC_000913/129262‑129459
                                                                                                  |                                                                                                   
  tgtgGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCaa                                                                                                  >  1:3315374/1‑100 (MQ=255)
  tgtgGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCaa                                                                                                  >  2:381367/1‑100 (MQ=255)
   gtgGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAAt                                                                                                 >  1:3823058/1‑100 (MQ=255)
   gtgGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAAt                                                                                                 <  1:1834890/100‑1 (MQ=255)
     gggCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTa                                                                                               <  1:2990852/100‑1 (MQ=255)
     gggCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTa                                                                                               >  1:521697/1‑100 (MQ=255)
     gggCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTa                                                                                               >  1:1870896/1‑100 (MQ=255)
     gggCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTa                                                                                               >  2:3737827/1‑100 (MQ=255)
     ggaccTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTa                                                                                               >  2:1621389/4‑100 (MQ=255)
      ggCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTTCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAAtt                                                                                                <  2:3077902/98‑1 (MQ=255)
      ggCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTaa                                                                                              >  2:2580720/1‑100 (MQ=255)
       gCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTaga                                                                                             >  1:2133667/1‑98 (MQ=255)
       gCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTaaa                                                                                             <  2:859059/100‑1 (MQ=255)
       gCCTGGCGGCACAAGTGTTCGAAGGTAGCATTACCGGCCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTaaa                                                                                             <  2:3629833/100‑1 (MQ=255)
        ccTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTaaaa                                                                                            <  1:85371/100‑1 (MQ=255)
         cTGGCGGCAGAAGTGTTCGAAGGTAGCCTTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTACTTTTTCGTTTGCCGGAACATCCGTCAATTaaaaa                                                                                           <  1:2887464/100‑1 (MQ=255)
         cTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTaaaaa                                                                                           <  1:769413/100‑1 (MQ=255)
              ggcAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCg                                                                                       <  2:878922/99‑1 (MQ=255)
                cAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCt                                                                                    >  1:3883575/1‑100 (MQ=255)
                cAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGc                                                                                     <  1:1083673/99‑1 (MQ=255)
                cAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCt                                                                                    <  1:259576/100‑1 (MQ=255)
                  gAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCACTTAAAAAAGCGGCTaa                                                                                  >  2:2420411/1‑100 (MQ=255)
                  gAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTaa                                                                                  >  2:398055/1‑100 (MQ=255)
                       gtTCGAAGGTAGCATTACCGACCTGCCGAACCCGACAGCAATGTAGCAGTAATTTTTCGTTTGCCCGGACATCGGGCAATTACACAAGCCACTaagtcc                                                                              >  2:2318901/1‑95 (MQ=255)
                       gtTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcg                                                                             >  1:3275415/1‑100 (MQ=255)
                       gtTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcg                                                                             >  1:2241489/1‑100 (MQ=255)
                       gtTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCAcg                                                                             >  1:429931/1‑100 (MQ=255)
                         tCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCTTTTTCTTTAACATCCGGCAATTAAAAAAGCGGCTAACCAcgcc                                                                           >  2:2686109/1‑100 (MQ=255)
                         tCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGcgtggttagcc                                                                           <  2:3898955/100‑11 (MQ=255)
                          cGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCAcgccg                                                                          >  2:3375996/1‑100 (MQ=255)
                          cGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCAcgccg                                                                          <  2:565699/100‑1 (MQ=255)
                           gAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcgccg                                                                          <  2:251355/99‑1 (MQ=255)
                            aaGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAGAAAAGCGGCTAACCACGCCGCt                                                                        <  2:922436/100‑1 (MQ=255)
                            aaGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCt                                                                        <  1:2461430/100‑1 (MQ=255)
                              ggTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCCATTAAAAAAGCGGCTAACCACGCCGCttt                                                                      >  2:1319780/1‑100 (MQ=255)
                               gTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCtttt                                                                     >  1:1778820/1‑100 (MQ=255)
                                ctgCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCttttt                                                                    <  2:1220462/98‑1 (MQ=255)
                                 aGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCtttttt                                                                   >  1:1875611/1‑100 (MQ=255)
                                  gCATTACCGACCTGCCGAACCCGAAAGCGAAGCAGCAGTACTTTTTCGTTTGCCGGAACCACCGCCAATTCAAAGAGCGGCTAACCACGCCGCttttttt                                                                  >  2:3367580/1‑100 (MQ=255)
                                  gCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCttttttt                                                                  <  2:264306/100‑1 (MQ=255)
                                  gCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCttttttt                                                                  >  2:3176221/1‑100 (MQ=255)
                                  gCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCtttttag                                                                  >  1:1220470/1‑98 (MQ=255)
                                   cATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTa                                                                 <  2:3275486/100‑1 (MQ=255)
                                    aTTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTTTTTTTTTTTTACCTCCGGCAAGTAAAAAACCGGCCAACCTCGCCGCTTTTGTTAc                                                                >  2:1983707/1‑100 (MQ=255)
                                    aTTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTAc                                                                <  2:4070141/100‑1 (MQ=255)
                                    aTTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTAc                                                                >  1:2743867/1‑100 (MQ=255)
                                     ttACCGACCTGCCGAACCCGAAAGCGAAGAAGACGTAATTTTTCGTTTTCCTGATCATCCAGCAATAACCTAAGCAGCTACCCGCGCCGCattctttt                                                                 >  2:2414874/1‑97 (MQ=255)
                                     ttACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACg                                                               <  1:969013/100‑1 (MQ=255)
                                       aCCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTc                                                             >  1:1005895/1‑100 (MQ=255)
                                         cGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTg                                                           <  2:1708959/100‑1 (MQ=255)
                                          gACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGc                                                          >  1:681666/1‑100 (MQ=255)
                                             cTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAAt                                                       <  1:601872/100‑1 (MQ=255)
                                                cctgaCCCGAAAGCGAACAAGAAGTAATTTTTCCTTTGCCGGAACATTCGTCAATTAAAAAAGCCTCTAACCACGCCGCTTTTTTTACGTCTGCAAttt                                                     <  2:2756132/95‑1 (MQ=255)
                                                ccGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTTCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTa                                                    >  2:1436421/1‑100 (MQ=255)
                                                 cGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTAc                                                   >  1:2967008/1‑100 (MQ=255)
                                                 cGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTAc                                                   >  1:129800/1‑100 (MQ=255)
                                                  gAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTAcc                                                  >  1:385961/1‑100 (MQ=255)
                                                   aaCCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCt                                                 >  1:834763/1‑100 (MQ=255)
                                                     cccGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCttt                                               >  1:3053845/1‑100 (MQ=255)
                                                     cccGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCttt                                               >  2:1575610/1‑100 (MQ=255)
                                                      ccGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTc                                              <  1:2554316/100‑1 (MQ=255)
                                                      ccGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTc                                              >  2:2936417/1‑100 (MQ=255)
                                                       cGAAAGCGAAGGAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCGCGCCGCTTTTTTTACGTCTGCAATTTACCTTTcc                                             >  2:2174486/1‑100 (MQ=255)
                                                       cGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTcc                                             <  1:463279/100‑1 (MQ=255)
                                                       cGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTcc                                             <  2:3420197/100‑1 (MQ=255)
                                                       cGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTcc                                             >  1:2729879/1‑100 (MQ=255)
                                                         aaaGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCac                                           <  2:1684072/100‑2 (MQ=255)
                                                         aaaGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAg                                           <  1:1736877/100‑1 (MQ=255)
                                                            gCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtct                                        <  2:2746595/100‑1 (MQ=255)
                                                             cGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtctt                                       >  1:3557734/1‑100 (MQ=255)
                                                              gaagaagaaGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtcttc                                      >  1:3401070/1‑100 (MQ=255)
                                                              gaagaagaaGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtcttc                                      >  1:2917797/1‑100 (MQ=255)
                                                               aagaagaagTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtcttct                                     <  1:1894972/100‑1 (MQ=255)
                                                               aagaagaagTAACTTGTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTCACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtcttct                                     <  2:2990848/100‑1 (MQ=255)
                                                                agaagaagTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTcttctt                                    >  2:3108269/1‑100 (MQ=255)
                                                                  aagaagTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGc                                  <  2:1357577/100‑1 (MQ=255)
                                                                  aagaagTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGc                                  <  1:2334432/100‑1 (MQ=255)
                                                                   agaagTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAACAGCGGCTACCCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCt                                 <  2:2408512/100‑1 (MQ=255)
                                                                   agaagTAATTTTTCGTTTGACGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGAAATTTACCTATCAAGACTTCTTgat                                 >  2:1915892/1‑98 (MQ=255)
                                                                     aagTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTcc                               >  1:2282113/1‑100 (MQ=255)
                                                                     aagTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTcc                               <  2:79854/100‑1 (MQ=255)
                                                                     aagTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTcc                               <  1:1557261/100‑1 (MQ=255)
                                                                      agTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTcc                               >  2:2091496/1‑99 (MQ=255)
                                                                       gTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCAc                             >  1:3745837/1‑100 (MQ=255)
                                                                         aaTTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGt                           >  1:2546203/1‑100 (MQ=255)
                                                                         aaTTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGt                           <  1:3018570/100‑1 (MQ=255)
                                                                           tttttCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTc                         <  1:2504739/100‑1 (MQ=255)
                                                                              ttCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCaga                      <  1:2436712/100‑1 (MQ=255)
                                                                                cGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCagaga                    >  1:219097/1‑100 (MQ=255)
                                                                                 gTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCagagag                   >  1:1616335/1‑100 (MQ=255)
                                                                                 gTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCagaga                    >  2:2041768/1‑99 (MQ=255)
                                                                                  tttGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAgagaga                  >  2:168587/1‑100 (MQ=255)
                                                                                     gCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGt               >  1:323730/1‑100 (MQ=255)
                                                                                       cGGAACATCCGGCAATTAAAAAAGCGACTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCTGAGAACCGTTc             >  2:1263170/1‑100 (MQ=255)
                                                                                        ggAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCg            <  2:3863524/100‑1 (MQ=255)
                                                                                        ggAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCg            <  1:1678088/100‑1 (MQ=255)
                                                                                         gAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGc           <  2:670431/100‑1 (MQ=255)
                                                                                          aaCATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCa          <  1:2432026/100‑1 (MQ=255)
                                                                                           aCATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCAt         <  1:3962233/100‑1 (MQ=255)
                                                                                                cGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATActgc    >  1:3619379/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
TCTGTGGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACTGCTG  >  NC_000913/129262‑129459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: