Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 569,160 G→T 19.4% D87Y (GAC→TAC) ‡ ybcK → DLP12 prophage; putative recombinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913569,1600GT19.4% 440.7 / 90.9 186D87Y (GAC→TAC) ybcKDLP12 prophage; putative recombinase
Reads supporting (aligned to +/- strand):  ref base G (71/79);  new base T (13/23);  total (84/102)
Fisher's exact test for biased strand distribution p-value = 2.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.88e-01

Only 100 of 196 total aligned reads displayed.

AAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCT  >  NC_000913/569061‑569259
                                                                                                   |                                                                                                   
aaaGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTTGTTGAAAGTCTgg                                                                                                     <  1:2805668/100‑1 (MQ=255)
aaaGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTgg                                                                                                     <  2:1060590/100‑1 (MQ=255)
aaaGGAAAGCATGCACAATCAGGAGCTTTTTCGGAACTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTgg                                                                                                     <  1:2901285/100‑1 (MQ=255)
  aGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGAc                                                                                                   <  2:119922/100‑1 (MQ=255)
  aGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGAc                                                                                                   <  1:1998317/100‑1 (MQ=255)
  aGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGAc                                                                                                   >  2:2668301/1‑100 (MQ=255)
   ggAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGAc                                                                                                   <  2:849015/99‑1 (MQ=255)
   ggAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACa                                                                                                  >  1:295723/1‑100 (MQ=255)
   ggAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACa                                                                                                  >  1:1492746/1‑100 (MQ=255)
    gAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAg                                                                                                 <  1:2585070/100‑1 (MQ=255)
     aaaGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGa                                                                                                <  2:2190920/100‑1 (MQ=255)
      aaGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGAc                                                                                               <  1:3494361/100‑1 (MQ=255)
        gCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACtt                                                                                             >  1:2499711/1‑100 (MQ=255)
          aTGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTc                                                                                           <  2:860419/100‑1 (MQ=255)
          aTGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTc                                                                                           <  1:1177209/100‑1 (MQ=255)
           tGCACAATCAGGATCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCa                                                                                          <  2:2594494/100‑1 (MQ=255)
           tGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCa                                                                                          >  1:3003214/1‑100 (MQ=255)
           tGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCa                                                                                          <  2:1850731/100‑1 (MQ=255)
            gCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCaa                                                                                         >  1:130595/1‑100 (MQ=255)
             cacaATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTCaac                                                                                        <  1:2603553/100‑2 (MQ=255)
             cacaATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTCaac                                                                                        <  1:1767061/100‑2 (MQ=255)
             cacaATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTCaac                                                                                        <  1:1642870/100‑2 (MQ=255)
             cacaATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTCaac                                                                                        <  1:3378141/100‑2 (MQ=255)
             cacaATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTCaac                                                                                        <  1:516879/100‑2 (MQ=255)
             cacaATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAag                                                                                        <  1:1266757/100‑1 (MQ=255)
             cacaATCAGGAGCTTTTTCGGAATTTTTAGATCCTATAGCGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTCaac                                                                                        <  2:2904560/100‑2 (MQ=255)
              acaATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAaga                                                                                       <  2:3876149/100‑1 (MQ=255)
               caATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTCaac                                                                                        <  2:1802848/98‑2 (MQ=255)
               caATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTCaac                                                                                        <  1:11201/98‑2 (MQ=255)
               caATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAagag                                                                                      >  1:4097602/1‑100 (MQ=255)
                     ggAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAg                                                                                >  1:2678368/1‑100 (MQ=255)
                     ggAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAg                                                                                >  2:2371893/1‑100 (MQ=255)
                        gCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGTCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCg                                                                             >  2:800547/1‑100 (MQ=255)
                        gCTTTTTCGGAATTTTTAGATGCTATAGAGCATCCTTATATATTGCCAGGAACTACATTGTTCGTTGAAAGTCTGGACAGACTATCAAGAaaaaaa                                                                                 >  2:4012251/1‑96 (MQ=255)
                         cTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCgg                                                                            <  1:2073235/100‑1 (MQ=255)
                         cTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCgg                                                                            <  1:1797693/100‑1 (MQ=255)
                           ttttCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTg                                                                          >  1:3325508/1‑100 (MQ=255)
                            tttCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGa                                                                         >  2:3547769/1‑100 (MQ=255)
                               cGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGc                                                                      <  2:1615625/100‑1 (MQ=255)
                               cGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGc                                                                      >  1:2355474/1‑100 (MQ=255)
                               cGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGc                                                                      >  2:3662982/1‑100 (MQ=255)
                               cGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGAAAAAAAAAAAAAAAAGTCGGTGAAGc                                                                      <  2:3003195/100‑1 (MQ=255)
                                ggAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCg                                                                     >  1:83513/1‑100 (MQ=255)
                                    tttttAGATGCTATAGAGCATGGTTATATATTGCCATGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGAtt                                                                  >  2:3061718/1‑99 (MQ=255)
                                    tttttAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTg                                                                 <  1:346930/100‑1 (MQ=255)
                                    tttttAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTg                                                                 <  1:2307654/100‑1 (MQ=255)
                                    tttttAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGAAAGAAAAAAAAAAAAAAAAGTCGGTGAAGCGAtt                                                                  <  2:2122669/99‑1 (MQ=255)
                                    tataaccaTGCTCTATAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTg                                                                 >  1:989015/8‑100 (MQ=255)
                                     ttttAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGa                                                                >  2:2798689/1‑100 (MQ=255)
                                         aGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGt                                                            >  2:2427576/1‑100 (MQ=255)
                                          gATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTc                                                           >  2:3789072/1‑100 (MQ=255)
                                           aTGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCt                                                          >  2:339927/1‑100 (MQ=255)
                                            tGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTg                                                         >  1:3739401/1‑100 (MQ=255)
                                            tGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTg                                                         <  1:2149829/100‑1 (MQ=255)
                                              cTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGaa                                                       <  2:2386199/100‑1 (MQ=255)
                                                 taGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAAtt                                                    <  2:3259913/100‑1 (MQ=255)
                                                 taGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAAtt                                                    >  2:1918361/1‑100 (MQ=255)
                                                   gagCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTg                                                   <  2:1265781/99‑1 (MQ=255)
                                                      cATGGTTAGATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGAttt                                               >  2:1636599/1‑100 (MQ=255)
                                                        tGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTg                                             <  1:3318972/100‑1 (MQ=255)
                                                          gTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGaa                                           >  1:3253437/1‑100 (MQ=255)
                                                              tatatTGCCAGGAACTACATTGTTAGTTGAAAGTCTTGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCAc                                       >  2:1599711/1‑100 (MQ=255)
                                                              tatatTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCAc                                       >  1:153129/1‑100 (MQ=255)
                                                               atatTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACg                                      >  2:3517271/1‑100 (MQ=255)
                                                                tatTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACgg                                     <  1:1918374/100‑1 (MQ=255)
                                                                tatTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACgg                                     <  1:3992092/100‑1 (MQ=255)
                                                                tatTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACgg                                     <  1:503563/100‑1 (MQ=255)
                                                                tatTGCAATGAACTACATTGTTAGTTCAAATGCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACgg                                     <  2:3891628/100‑1 (MQ=255)
                                                                       aGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGAt                              <  1:1845018/100‑1 (MQ=255)
                                                                        ggAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATg                             <  2:4106903/100‑1 (MQ=255)
                                                                        ggAACTACATTGTTAGTGGAAAGGCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATg                             <  2:1883987/100‑1 (MQ=255)
                                                                           aCTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTta                          <  1:3663006/100‑1 (MQ=255)
                                                                             tACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTtata                        >  1:1735366/1‑100 (MQ=255)
                                                                             tACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTtata                        <  2:3194711/100‑1 (MQ=255)
                                                                               cATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAAc                      >  2:3814070/1‑100 (MQ=255)
                                                                               cATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAAc                      <  2:507528/100‑1 (MQ=255)
                                                                                  tGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAActct                   >  1:1047955/1‑100 (MQ=255)
                                                                                   gTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCtt                  <  1:1718448/100‑1 (MQ=255)
                                                                                   gTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCtt                  >  1:1382782/1‑100 (MQ=255)
                                                                                      tcttGAAAGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGCTTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGc               >  2:2286137/3‑100 (MQ=39)
                                                                                      aGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGc               <  1:2495784/100‑1 (MQ=255)
                                                                                      aGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGc               <  1:3601837/100‑1 (MQ=255)
                                                                                       gTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCg              >  2:3057306/1‑100 (MQ=255)
                                                                                       cttGAAAGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCg              >  1:1444000/2‑100 (MQ=255)
                                                                                       cttGAAAGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCg              >  1:92617/2‑100 (MQ=255)
                                                                                        ttGAAAGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGa             >  2:955413/1‑100 (MQ=255)
                                                                                        ttGAAAGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGa             >  1:2014020/1‑100 (MQ=255)
                                                                                         tGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGAc            <  2:230427/100‑1 (MQ=255)
                                                                                         tGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGAc            <  1:1617754/100‑1 (MQ=255)
                                                                                         tGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGAc            >  1:1578172/1‑100 (MQ=255)
                                                                                            aaGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAAt         <  2:2014007/100‑1 (MQ=255)
                                                                                            aaGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAAt         <  1:748357/100‑1 (MQ=255)
                                                                                             aGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATa        >  2:2848596/1‑100 (MQ=255)
                                                                                             aGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATa        <  1:263851/100‑1 (MQ=255)
                                                                                              gTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATAc       >  2:1180382/1‑100 (MQ=255)
                                                                                                cTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAg     <  2:1914317/100‑1 (MQ=255)
                                                                                                  ggACAGACTTTCAATAGAAAAAGTCGGTGAAGCGATTGAACGTCTGTAATTGATTTTGAATCACTGTATTGATGTTATAACTCTTTGCGACAATACAGTc   >  2:3715099/1‑100 (MQ=255)
                                                                                                  ggACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTc   <  1:1072145/100‑1 (MQ=255)
                                                                                                  ggACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGAATGAACGTCTGAAATCGAGTTTGAATCACGGTATTAATGTTATAACTCTTTGCGACAATACAGTc   >  2:2489478/1‑100 (MQ=255)
                                                                                                   gACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCt  <  2:678174/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
AAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCT  >  NC_000913/569061‑569259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: