Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 654,566 G→T 15.8% intergenic (+24/+17) citB → / ← dcuC response regulator in two‑component regulatory system with CitA/anaerobic C4‑dicarboxylate transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913654,5660GT15.8% 507.2 / 76.9 196intergenic (+24/+17)citB/dcuCresponse regulator in two‑component regulatory system with CitA/anaerobic C4‑dicarboxylate transport
Reads supporting (aligned to +/- strand):  ref base G (69/96);  new base T (17/14);  total (86/110)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.99e-01

Only 100 of 202 total aligned reads displayed.

CAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCG  >  NC_000913/654467‑654665
                                                                                                   |                                                                                                   
cAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTg                                                                                                     <  2:1058374/100‑1 (MQ=255)
cAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTg                                                                                                     <  2:3506133/100‑1 (MQ=255)
cAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTg                                                                                                     <  1:1732353/100‑1 (MQ=255)
  gccgccATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGcc                                                                                                   <  2:604288/100‑1 (MQ=255)
  gccgccATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGcc                                                                                                   >  1:3361563/1‑100 (MQ=255)
  gccgccATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGcc                                                                                                   >  1:128086/1‑100 (MQ=255)
    cgccATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGccgc                                                                                                 >  2:2504979/1‑98 (MQ=255)
        aTCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGGTAAAGCCGGAGGAAACTTGCCTCCGGc                                                                                             >  1:3046589/1‑100 (MQ=255)
        aTCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTCCTCCGGc                                                                                             <  2:3037444/100‑1 (MQ=255)
        aTCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTCCTCCGGc                                                                                             <  2:3544129/100‑1 (MQ=255)
        aTCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGc                                                                                             <  2:1423136/100‑1 (MQ=255)
         tCTGCTTATTCCTGAAATTGTTCACGGCAACGTTGGCAGACCACAACGCATATACCCCAGTGGGTCACATGATGAATCCGAAGGAAACTTGCCTCCGCCa                                                                                            >  2:3650261/1‑100 (MQ=255)
         tCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTCCTCCGGct                                                                                            <  1:1045733/100‑2 (MQ=255)
         tCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTCCTCCGGct                                                                                            <  1:3512537/100‑2 (MQ=255)
         tCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTCCTCCGGct                                                                                            <  1:1699424/100‑2 (MQ=255)
         tCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCa                                                                                            <  2:3152690/100‑1 (MQ=255)
         tCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCa                                                                                            <  1:1562139/100‑1 (MQ=255)
          cTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTCCTCCGGctt                                                                                           <  2:2689872/100‑3 (MQ=255)
            gactattGCTTGAATTGTTCCCGACAAAGGGGGCAGACCACACCTCATAGACCACAGTGGGTGAAATGATAAAGCCGGAGGAAACTTGCCTCCGGCAtt                                                                                          <  2:4128872/96‑1 (MQ=255)
             attattGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTAc                                                                                        <  2:3361543/100‑1 (MQ=255)
                   gCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTAttt                                                                                   <  1:3398260/99‑1 (MQ=255)
                    cTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTAc                                                                                 <  2:3772057/100‑1 (MQ=255)
                    cTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTAc                                                                                 >  2:3433654/1‑100 (MQ=255)
                    cTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTAc                                                                                 >  1:1346889/1‑100 (MQ=255)
                      gAAATTGTTCACGGCAAAGTTGGCAGACCACAAAGCATATACCACAGCCGGTGGCATTATAACGCCGGAGGAAACTTGCCTCCGGCAGTACTATTTAc                                                                                 >  2:213457/1‑98 (MQ=255)
                       aaaTTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTg                                                                              <  1:1320832/100‑1 (MQ=255)
                        aaTTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGc                                                                             >  2:3462634/1‑100 (MQ=255)
                           tGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCtg                                                                          <  1:1839516/100‑1 (MQ=255)
                            gTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCtgt                                                                         >  2:3112733/1‑100 (MQ=255)
                            gTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCtgt                                                                         >  1:2887035/1‑100 (MQ=255)
                             ttCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCtgtg                                                                        <  1:3955009/100‑1 (MQ=255)
                              tCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGa                                                                       >  1:3029751/1‑100 (MQ=255)
                               cACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGAc                                                                      <  1:2720180/100‑1 (MQ=255)
                               cACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGAc                                                                      >  1:4095151/1‑100 (MQ=255)
                                   gCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgct                                                                  >  2:2992422/1‑100 (MQ=255)
                                    cAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgctg                                                                 <  2:2797477/100‑1 (MQ=255)
                                     aaaGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgctgc                                                                <  2:1279656/100‑1 (MQ=255)
                                     aaaGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgctgc                                                                <  2:1346880/100‑1 (MQ=255)
                                      aaGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgctgct                                                               >  1:120100/1‑100 (MQ=255)
                                        gTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCtgctgc                                                             <  1:1755276/100‑1 (MQ=255)
                                         ttGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGcc                                                            <  1:229519/100‑1 (MQ=255)
                                          tGGCAGACCACCACGCATATACCACTGTGGCTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCg                                                           <  2:2350567/100‑1 (MQ=255)
                                          tGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCg                                                           <  1:1233580/100‑1 (MQ=255)
                                           ggCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGt                                                          >  2:3819649/1‑100 (MQ=255)
                                            gCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGtt                                                         >  1:3642688/1‑100 (MQ=255)
                                            gCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGtt                                                         <  1:382791/100‑1 (MQ=255)
                                             cAGACCACAACGCATATACCACAGTGGGTGACATGATAATGTCGGAGGTAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCg                                                           <  2:3710170/97‑1 (MQ=255)
                                              aGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTcc                                                       <  1:30958/100‑1 (MQ=255)
                                                aCCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTg                                                     <  2:3912596/100‑1 (MQ=255)
                                                aCCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTg                                                     <  1:324714/100‑1 (MQ=255)
                                                  cacaACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGc                                                   <  1:2940608/100‑1 (MQ=255)
                                                    caACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCAc                                                 <  2:3765261/100‑1 (MQ=255)
                                                    caACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTAACCGCTGCTGCCGTTCCTGGCAc                                                 >  2:3545101/1‑100 (MQ=255)
                                                       cGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCAcc                                                <  2:3459358/98‑1 (MQ=255)
                                                          ctataCCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAg                                           <  2:1142641/99‑1 (MQ=255)
                                                           tataCCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGc                                          <  2:1943505/100‑1 (MQ=255)
                                                           tataCCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGc                                          <  2:1467121/100‑1 (MQ=255)
                                                            ataCCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAgag                                         >  1:1142648/1‑98 (MQ=255)
                                                            ataCCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGct                                         <  1:4000612/100‑1 (MQ=255)
                                                             taCCACAGTGGGTGACATGATAACGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGctc                                        <  1:2662012/100‑1 (MQ=255)
                                                             taCCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGctc                                        <  1:249437/100‑1 (MQ=255)
                                                             taCCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGctc                                        <  1:3910734/100‑1 (MQ=255)
                                                              aCCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGctct                                       <  2:2838147/100‑1 (MQ=255)
                                                               ccACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGctcag                                      >  2:2501307/1‑98 (MQ=255)
                                                               ccACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTg                                      >  1:532086/1‑100 (MQ=255)
                                                               ccACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTg                                      >  1:517187/1‑100 (MQ=255)
                                                                cacaGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGt                                     >  2:3563502/1‑100 (MQ=255)
                                                                  caGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTaa                                   >  1:703590/1‑99 (MQ=255)
                                                                  aaGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAg                                   <  2:792545/99‑1 (MQ=255)
                                                                       ggTGATATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCTCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACg                              <  2:2549461/100‑1 (MQ=255)
                                                                       ggTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACg                              >  1:1163218/1‑100 (MQ=255)
                                                                           aaatagtAATGCCGTAGGCAAGTTTCCTCCGGCTTTACTATGTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCACGTCTGTAGCAACGATaa                          >  2:2408597/7‑100 (MQ=21)
                                                                            cATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAAc                         <  1:1577159/100‑1 (MQ=255)
                                                                            cATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGAACGCTGCTGCCGTTCCTGGCACCATCAGCGCTGTAGCAACGATAAc                         >  2:1513394/1‑100 (MQ=255)
                                                                             aTGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACa                        <  2:1617333/100‑1 (MQ=255)
                                                                             aTGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACa                        <  1:2239202/100‑1 (MQ=255)
                                                                              tGATATACCCGGAGCAAACTTGCCTCCGTCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACa                        <  2:324015/99‑1 (MQ=255)
                                                                                gtAATGCCGGAGGCAAGTTTCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATc                     >  1:2081774/2‑100 (MQ=38)
                                                                                 tAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCa                    >  1:1869681/1‑100 (MQ=255)
                                                                                 tAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCa                    >  2:2026696/1‑100 (MQ=255)
                                                                                 tAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCa                    >  1:2569238/1‑100 (MQ=255)
                                                                                  aatgCCGGAGGCAAGTTTCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCac                   >  2:3833476/4‑100 (MQ=38)
                                                                                  aaaGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCa                    <  2:509765/99‑1 (MQ=255)
                                                                                   ntgCCGGAGGCAAGTTTCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCacc                  >  1:1297324/3‑100 (MQ=255)
                                                                                    aGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCacca                 >  1:2044107/1‑100 (MQ=255)
                                                                                    aGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCacca                 >  1:1524615/1‑100 (MQ=255)
                                                                                     gCCGGAGGCACGTTTCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCACTGTAGCAACGATAACAATCacca                 >  2:1208454/1‑99 (MQ=255)
                                                                                     gCCGGAGGCAAGTTTGCTCCGGCATTACTATTTACTTGCCTGGGACCGCTGCTGCCATTCCTAGACCCACAAGCTATTTAGCAAAAACAACACTCgccaa                >  2:740796/1‑100 (MQ=255)
                                                                                     gCCGGAGGCAAGTTTCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCaccaa                >  2:3577667/1‑100 (MQ=255)
                                                                                     gCCGGAGGCAAGTTTCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCaccaa                >  1:45672/1‑100 (MQ=255)
                                                                                      ccgcaagaAACTTGCCTCCGCCATTACTATTTACCTGCCTGTGACCGCTGCTGCCGTTCCTTGCACCATCAGCTCTGTAGCAACGATAACAATCaccaaa               <  2:4071559/94‑1 (MQ=255)
                                                                                      ccGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCaccaaa               <  1:1655137/100‑1 (MQ=255)
                                                                                      ccGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCaccaaa               <  2:4049559/100‑1 (MQ=255)
                                                                                       cGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCaccaaac              <  1:76732/100‑1 (MQ=255)
                                                                                        ggaggaAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCaccaaacc             <  1:1412567/100‑1 (MQ=255)
                                                                                               aCTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGc      <  1:442963/100‑1 (MQ=255)
                                                                                                 ttGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCAc    <  1:1149209/100‑1 (MQ=255)
                                                                                                  tGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCAcc   >  1:3291758/1‑100 (MQ=255)
                                                                                                  tGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCAcc   >  1:3085802/1‑100 (MQ=255)
                                                                                                   gCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCg  >  1:2126126/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCG  >  NC_000913/654467‑654665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: