Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 676,403 C→T 56.5% A49T (GCC→ACC)  ybeQ ← Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913676,4030CT56.5% 35.5 / 182.0 145A49T (GCC→ACC) ybeQSel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base C (30/33);  new base T (45/37);  total (75/70)
Fisher's exact test for biased strand distribution p-value = 4.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.29e-02

Only 100 of 152 total aligned reads displayed.

TCTTTATGACGGCTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCACGTTCGATAAT  >  NC_000913/676306‑676502
                                                                                                 |                                                                                                   
tCTTTATGACGGCTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGttt                                                                                                   <  2:2224314/100‑1 (MQ=255)
tCTTTATGACGGCTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGttt                                                                                                   <  1:3158134/100‑1 (MQ=255)
    tATGACGGCTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTtc                                                                                               >  2:373638/1‑100 (MQ=255)
      tGACGGCTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTtcgt                                                                                             >  1:284048/1‑100 (MQ=255)
      tGACGGCTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTtcgt                                                                                             <  2:602042/100‑1 (MQ=255)
       gACGGCTGTTAGCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTtcgtc                                                                                            >  2:2172678/1‑100 (MQ=255)
        aCGGCTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTtcgtcg                                                                                           >  1:3310985/1‑100 (MQ=255)
         cGGCTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTcgtcgt                                                                                          >  1:144254/1‑100 (MQ=255)
            cTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTcc                                                                                        >  2:3084896/1‑99 (MQ=255)
            cTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCg                                                                                       >  2:359957/1‑100 (MQ=255)
            cTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCg                                                                                       >  1:2866693/1‑100 (MQ=255)
            cTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCg                                                                                       <  1:1009738/100‑1 (MQ=255)
            cTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCg                                                                                       <  1:2082254/100‑1 (MQ=255)
              tttATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGga                                                                                     <  1:2537584/100‑1 (MQ=255)
               ttATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGgag                                                                                    <  1:725499/100‑1 (MQ=255)
                tATCTTCGGTATACGTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCgg                                                                                      >  2:2250942/1‑97 (MQ=255)
                tATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGgaga                                                                                   >  2:1483530/1‑100 (MQ=255)
                 aTCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGgaga                                                                                   >  2:1019559/1‑99 (MQ=255)
                  tCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAAt                                                                                 >  2:365472/1‑100 (MQ=255)
                   ccTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATc                                                                                >  1:3718305/1‑100 (MQ=255)
                   ccTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATc                                                                                >  1:69208/1‑100 (MQ=255)
                      cGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAAt                                                                             <  1:491009/100‑1 (MQ=255)
                      cGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAAt                                                                             <  2:386748/100‑1 (MQ=255)
                       ggTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAAtt                                                                            >  1:813141/1‑100 (MQ=255)
                        gTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTg                                                                           >  2:444849/1‑100 (MQ=255)
                         tataCTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTgc                                                                          <  1:2456508/100‑1 (MQ=255)
                           taCTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTgcgc                                                                        <  1:3186679/100‑1 (MQ=255)
                           taCTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTgcgc                                                                        <  2:284632/100‑1 (MQ=255)
                            aCTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCt                                                                       >  2:70963/1‑100 (MQ=255)
                             cTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTa                                                                      >  2:40916/1‑100 (MQ=255)
                                  gCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATcgcg                                                                 <  1:3132242/100‑1 (MQ=255)
                                   cccTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGAAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGa                                                                >  2:1601919/1‑100 (MQ=255)
                                    ccTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGAt                                                               >  2:834042/1‑100 (MQ=255)
                                     cTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGAtt                                                              <  2:3742200/100‑1 (MQ=255)
                                       aaGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATtat                                                            <  1:2041834/100‑1 (MQ=255)
                                        aGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATtata                                                           <  1:4125954/100‑1 (MQ=255)
                                        aGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATtata                                                           <  1:2925945/100‑1 (MQ=255)
                                         ggAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTataa                                                          <  2:3135967/100‑1 (MQ=255)
                                           aaTACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTataata                                                        <  1:3805384/100‑1 (MQ=255)
                                             tACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATaaa                                                      <  2:71874/100‑1 (MQ=255)
                                              aCTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATaaaa                                                     >  2:1777990/1‑100 (MQ=255)
                                              aCTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATaaaa                                                     >  2:572631/1‑100 (MQ=255)
                                               cTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAAc                                                    <  2:594551/100‑1 (MQ=255)
                                               cTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAAc                                                    <  1:460349/100‑1 (MQ=255)
                                                 gtgCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGTTTATAATAAAAcc                                                   >  2:2817894/1‑99 (MQ=255)
                                                   gCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAAccc                                                  >  2:1619531/1‑98 (MQ=255)
                                                   gCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCcaa                                                >  2:350884/1‑100 (MQ=255)
                                                        acaATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAata                                           <  1:799694/100‑1 (MQ=255)
                                                        acaATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAata                                           >  1:2694834/1‑100 (MQ=255)
                                                        acaATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAata                                           <  1:2341686/100‑1 (MQ=255)
                                                        acaATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAata                                           <  2:140556/100‑1 (MQ=255)
                                                            tGACCTTGCTCAGCGGCCAGCTTCAGACAGTAAAAGGTTTGTTCGTCGACCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATAtt                                       <  2:1908886/100‑1 (MQ=255)
                                                             gACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTg                                      <  2:612015/100‑1 (MQ=255)
                                                              aCCTTGCTGAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATGGCGATTATAATAAAAACCAACAATATATTg                                      >  2:2602128/1‑99 (MQ=255)
                                                              aCCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGa                                     >  1:2855311/1‑100 (MQ=255)
                                                              aCCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGa                                     <  2:2655567/100‑1 (MQ=255)
                                                               ccTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAg                                    >  2:3309618/1‑100 (MQ=255)
                                                               ccTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAg                                    >  1:3928762/1‑100 (MQ=255)
                                                               ccTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACGCAACAATATATTGAg                                    >  1:4068136/1‑100 (MQ=255)
                                                               ccTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAg                                    >  1:2222467/1‑100 (MQ=255)
                                                                  tGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCt                                 <  1:2335910/100‑1 (MQ=255)
                                                                    cTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCg                               <  2:494115/100‑1 (MQ=255)
                                                                    cTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCg                               <  2:2378393/100‑1 (MQ=255)
                                                                    cTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCt                                 >  2:1178480/1‑98 (MQ=255)
                                                                     tCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGc                              >  1:2701186/1‑100 (MQ=255)
                                                                     tCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGc                              <  1:1025385/100‑1 (MQ=255)
                                                                     tCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGc                              <  1:3761413/100‑1 (MQ=255)
                                                                      cAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCa                             <  2:4068109/100‑1 (MQ=255)
                                                                        gCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGAGTATAATAAAACCCAACAATATATTGAGCCTCGCaa                            >  2:3446473/1‑99 (MQ=255)
                                                                          ggCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCg                         >  1:1664301/1‑100 (MQ=255)
                                                                          ggCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCg                         <  2:2098509/100‑1 (MQ=255)
                                                                          ggCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCg                         <  2:3012008/100‑1 (MQ=255)
                                                                          ggCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCg                         >  1:707503/1‑100 (MQ=255)
                                                                          ggCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTGGCAATCg                         <  1:2172691/100‑1 (MQ=255)
                                                                           gCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGc                        >  1:1764973/1‑100 (MQ=255)
                                                                           gCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCg                         >  2:268190/1‑99 (MQ=255)
                                                                           gCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGc                        >  2:1145168/1‑100 (MQ=255)
                                                                             cAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCt                      >  2:2399756/1‑100 (MQ=255)
                                                                             cAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCt                      <  2:2969998/100‑1 (MQ=255)
                                                                               gCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAAGCCAACAATATATTGAGCCTCGCAATCGCCttt                    >  2:2062221/1‑100 (MQ=255)
                                                                                cTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCtttt                   <  2:1877659/100‑1 (MQ=255)
                                                                                  tCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTc                 <  2:3320611/100‑1 (MQ=255)
                                                                                   cAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCa                <  1:2594583/100‑1 (MQ=255)
                                                                                   cAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCa                <  1:3382009/100‑1 (MQ=255)
                                                                                    aGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATCATAAAACCCACCAATATATTGCGCCTCGCAATCTCCtttttaa                >  2:678305/1‑97 (MQ=255)
                                                                                     gCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGc              >  1:1461515/1‑100 (MQ=255)
                                                                                     gCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGc              <  2:785775/100‑1 (MQ=255)
                                                                                     gCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGc              >  1:387244/1‑100 (MQ=255)
                                                                                     gCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGc              >  2:296062/1‑100 (MQ=255)
                                                                                     gCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGc              >  1:3246888/1‑100 (MQ=255)
                                                                                     gCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGc              >  1:606001/1‑100 (MQ=255)
                                                                                        aGTAAAAGGTTTTTTTTTCTTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCACg           >  2:3883991/1‑100 (MQ=255)
                                                                                        aGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCACg           >  1:1759835/1‑100 (MQ=255)
                                                                                           aaaaGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCACGTTc        >  1:3086755/1‑100 (MQ=255)
                                                                                            aaaGGTTTTTTCGTCGTCCGGAGCATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCACGTTc        >  1:1743644/1‑99 (MQ=255)
                                                                                            aaaGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCACGTTCg       >  1:883817/1‑100 (MQ=255)
                                                                                            aaaGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCACGTTCg       <  1:861745/100‑1 (MQ=255)
                                                                                             aaGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCACGTTCGa      <  1:2221624/100‑1 (MQ=255)
                                                                                             aaGGCTTTTTCGTCGTCCGGAGAATCAAGTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCACGTTCGa      <  1:2238145/100‑1 (MQ=255)
                                                                                                 cTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCACGTTCGATAAt  >  2:2490108/1‑100 (MQ=255)
                                                                                                 |                                                                                                   
TCTTTATGACGGCTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCACGTTCGATAAT  >  NC_000913/676306‑676502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: