Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 681,694 A→G 15.9% intergenic (+31/+29) djlC → / ← hscC J domain‑containing HscC co‑chaperone; Hsc56/Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913681,6940AG15.9% 337.2 / 53.2 145intergenic (+31/+29)djlC/hscCJ domain‑containing HscC co‑chaperone; Hsc56/Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor
Reads supporting (aligned to +/- strand):  ref base A (56/66);  new base G (12/11);  total (68/77)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.36e-01

Only 100 of 153 total aligned reads displayed.

ATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTG  >  NC_000913/681595‑681793
                                                                                                   |                                                                                                   
aTCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCa                                                                                                     >  1:673010/1‑100 (MQ=255)
  cAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGc                                                                                                   <  1:3161944/100‑1 (MQ=255)
       tCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGtaaa                                                                                              <  1:1526003/100‑4 (MQ=255)
         aGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcg                                                                                            <  1:1226084/100‑1 (MQ=255)
         aGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcg                                                                                            >  2:3645216/1‑100 (MQ=255)
          gTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcgc                                                                                           >  1:2630164/1‑100 (MQ=255)
          gTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGAGCCGATGCCGGATGCAGCGTTTAcgc                                                                                           >  1:2831011/1‑100 (MQ=255)
            ttAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcgccg                                                                                         <  1:884391/100‑1 (MQ=255)
              aaCGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCa                                                                                       >  2:1595708/1‑100 (MQ=255)
                cGGTGCTGGTGATAGGTTATGCTCTCTGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATc                                                                                     <  2:664131/100‑1 (MQ=38)
                 ggtggtggtGATAGGTTATGGTCTCGGCAAAATCGGGATGTTGTTGAGCTGACGATGTGTTTTTTGCGTCGATGCCGGATGCGGCGTAAACGCTGCATcc                                                                                    <  2:3157935/100‑1 (MQ=25)
                 ggTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATcc                                                                                    <  2:2151881/100‑1 (MQ=38)
                 ggTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATcc                                                                                    >  1:4064710/1‑100 (MQ=255)
                  gTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCg                                                                                   <  1:1830478/100‑1 (MQ=255)
                  gTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCg                                                                                   <  1:3622450/100‑1 (MQ=255)
                   tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                  <  2:1926432/100‑1 (MQ=38)
                   tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                  <  2:409622/100‑1 (MQ=38)
                    gCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGc                                                                                 <  2:939407/100‑1 (MQ=255)
                    gCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGc                                                                                 >  1:2767739/1‑100 (MQ=255)
                     cTGGTGATGGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCGGCa                                                                                <  2:1393560/100‑1 (MQ=37)
                     cTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCa                                                                                <  2:1863546/100‑1 (MQ=255)
                      tGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAg                                                                               <  1:773321/100‑1 (MQ=255)
                      tGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAg                                                                               <  1:3547577/100‑1 (MQ=255)
                      tGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAg                                                                               >  2:2390765/1‑100 (MQ=255)
                        gttagaGGTTAGGCTCTCTGCAAAAGCTCGATGTTATTTAGCTGACGATGTGTTATTTGCGCCTACGCCGTATGCAGCGTAAACCCTGCATCCGGc                                                                                 <  2:1169103/91‑1 (MQ=255)
                        gTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGcc                                                                             >  1:2865193/1‑100 (MQ=255)
                         tGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCg                                                                            <  2:4134129/100‑1 (MQ=255)
                             aGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGtt                                                                        >  2:1013240/1‑100 (MQ=255)
                              ggTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGttt                                                                       >  1:427620/1‑100 (MQ=255)
                              ggTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCAGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGttt                                                                       >  1:2908586/1‑100 (MQ=255)
                               gTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTa                                                                      >  1:1376653/1‑100 (MQ=255)
                               gTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTa                                                                      >  2:4003752/1‑100 (MQ=255)
                                ttATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTAt                                                                     >  1:1840773/1‑100 (MQ=255)
                                   tGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATggg                                                                  <  1:1103556/100‑1 (MQ=255)
                                   tGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATggg                                                                  >  1:3381725/1‑100 (MQ=255)
                                    gCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGa                                                                 <  2:288028/100‑1 (MQ=255)
                                     ctctCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAt                                                                <  1:1092879/100‑1 (MQ=255)
                                     ctctCGGCAAAATCACGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGAGTGCAGCGTTTACACCGCATCCGGCAGCCTTGTTTATGGTAt                                                                >  2:989738/1‑100 (MQ=255)
                                            cAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATTCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGACCTCAATg                                                         >  1:1320098/1‑100 (MQ=255)
                                            cAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATg                                                         <  2:799149/100‑1 (MQ=255)
                                            cAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCAGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATg                                                         >  1:3080411/1‑100 (MQ=255)
                                              aaaTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGc                                                       <  1:384509/100‑1 (MQ=255)
                                              aaaTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGc                                                       <  1:3851682/100‑1 (MQ=255)
                                                aTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTa                                                     <  2:3682409/100‑1 (MQ=255)
                                                   gcgATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAaat                                                  <  2:1540328/100‑1 (MQ=255)
                                                      aTGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCTGCAGCCGTGTTTATGGGATCTCAATGGCTAaataat                                               <  1:2920506/100‑1 (MQ=255)
                                                      aTGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGAGGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAaataat                                               <  2:95872/100‑1 (MQ=255)
                                                        gttgttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcg                                             <  1:2241379/100‑1 (MQ=255)
                                                        gttgttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcg                                             <  2:2686644/100‑1 (MQ=255)
                                                         ttgttTAGCTTACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCTGTATTTATGGGATCTCAATGGCTAAATAAtcgc                                            <  2:2715506/100‑1 (MQ=255)
                                                          tgttTAGCTGACGATGTGTTTTTTGCGTCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgct                                           <  2:2459679/100‑1 (MQ=255)
                                                          tgttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgct                                           >  1:2957577/1‑100 (MQ=255)
                                                          tgttTAGCTGACGATGGGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgct                                           <  1:961919/100‑1 (MQ=255)
                                                           gttTAGCTGACGATGTGTTTTTTGCTCCGATTCCGGATGCAGAGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgctc                                          >  2:1958692/1‑100 (MQ=255)
                                                              tAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCTGCATCCGGCAGCCGCGTTTATGGGATCTCAACGGCTAAATAAtcgctcgc                                        >  2:3400715/1‑99 (MQ=255)
                                                              tAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcc                                       <  2:1840760/100‑1 (MQ=255)
                                                               aGCTGACGATGTGTTTTTTGCTGAAATGCCAGATGCAGCGTTTATGCCGGATCCGGCAGCCGTGTTTATGGGATTTCACTGGCTGaataa                                                >  2:1082777/1‑90 (MQ=255)
                                                               aGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgccc                                      >  1:3705947/1‑100 (MQ=255)
                                                                 cTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccgc                                    >  1:492892/1‑100 (MQ=255)
                                                                 cTGACGATGCGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGACGTGTTTATAGGATCTCAATGGCTAAATAAtcgct                                           >  2:2796422/1‑93 (MQ=255)
                                                                  tGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccgcc                                   <  1:1291280/100‑1 (MQ=255)
                                                                     cGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCccgccgc                                 <  1:1012267/99‑1 (MQ=255)
                                                                      gATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCTCTCGCccgccgccg                               >  1:1765375/1‑100 (MQ=255)
                                                                       aTGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGa                              <  1:1284447/100‑1 (MQ=255)
                                                                         gtgtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATGCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCTGCCGCCGAAc                            <  2:2908570/100‑1 (MQ=255)
                                                                         gtgtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAAc                            <  1:392185/100‑1 (MQ=255)
                                                                           gtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACtt                          >  1:1014799/1‑100 (MQ=255)
                                                                           gtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACtt                          >  1:2392684/1‑100 (MQ=255)
                                                                             tttttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCa                        <  2:1074983/100‑1 (MQ=255)
                                                                              ttttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCAt                       <  2:619051/100‑1 (MQ=255)
                                                                               tttGCGGCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATc                      <  2:486512/100‑1 (MQ=255)
                                                                                 ctcgcCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCa                    <  1:1640314/98‑1 (MQ=255)
                                                                                   cgcCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCAcg                  >  2:2972113/1‑100 (MQ=255)
                                                                                     ccGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTACATAATCGCTCGCCCGCCGCCGAACTTCATCCAcgc                 >  2:4046079/1‑99 (MQ=255)
                                                                                     ccGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCAcgcg                >  1:3902521/1‑100 (MQ=255)
                                                                                     ccGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACATCATcc                     >  2:2592972/1‑95 (MQ=255)
                                                                                       gATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCggg              <  1:1081139/100‑1 (MQ=255)
                                                                                       gATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCggg              <  1:913961/100‑1 (MQ=255)
                                                                                        aTGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGc             <  1:1003441/100‑1 (MQ=255)
                                                                                         tGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGcc            >  1:1425197/1‑100 (MQ=255)
                                                                                         tGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGcc            <  2:45596/100‑1 (MQ=255)
                                                                                          gCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCa           >  1:3634275/1‑100 (MQ=255)
                                                                                           ccGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCat          >  1:3131924/1‑100 (MQ=38)
                                                                                           ccGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCat          >  1:1254775/1‑100 (MQ=255)
                                                                                           ccGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCACCGCCGAACTTCATCCACGCGGGCCat          >  2:748184/1‑100 (MQ=255)
                                                                                            cGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCata         >  1:1925008/1‑100 (MQ=38)
                                                                                            cGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCata         >  1:3096709/1‑100 (MQ=38)
                                                                                            cGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCata         >  1:613085/1‑100 (MQ=38)
                                                                                             ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGcc            >  1:843317/1‑96 (MQ=38)
                                                                                             ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat        >  1:2658191/1‑100 (MQ=38)
                                                                                             ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat        >  1:1171060/1‑100 (MQ=38)
                                                                                             ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCata         >  2:1689573/1‑99 (MQ=38)
                                                                                             ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACATCATCCACGCGGGCCatat        >  1:1927632/1‑100 (MQ=38)
                                                                                             ggATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAGCTTCATCCACGCGGGCCatat        >  1:940894/1‑100 (MQ=255)
                                                                                                 gCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGtt    <  1:3413939/100‑1 (MQ=255)
                                                                                                  cAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGttt   >  1:2545185/1‑100 (MQ=255)
                                                                                                  cAGCGTGGACTCCGCATCCGGCAGCCGTGTTTATGTGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGttt   <  1:1787368/100‑1 (MQ=255)
                                                                                                   aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGCACTTCATCCACGCGGGCCATATCGTTTg  >  1:879471/1‑100 (MQ=255)
                                                                                                   aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg  <  1:1207304/100‑1 (MQ=255)
                                                                                                   aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg  <  2:3282284/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
ATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTG  >  NC_000913/681595‑681793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: