Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 681,699 T→A 15.1% intergenic (+36/+24) djlC → / ← hscC J domain‑containing HscC co‑chaperone; Hsc56/Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913681,6990TA15.1% 399.2 / 54.0 153intergenic (+36/+24)djlC/hscCJ domain‑containing HscC co‑chaperone; Hsc56/Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor
Reads supporting (aligned to +/- strand):  ref base T (57/72);  new base A (12/11);  total (69/84)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

Only 100 of 156 total aligned reads displayed.

AGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGC  >  NC_000913/681604‑681798
                                                                                               |                                                                                                   
aGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcg                                                                                                 <  1:1226084/100‑1 (MQ=255)
 gTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcgc                                                                                                >  1:2650533/1‑100 (MQ=255)
 gTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcgc                                                                                                >  1:2630164/1‑100 (MQ=255)
 gTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGAGCCGATGCCGGATGCAGCGTTTAcgc                                                                                                >  1:2831011/1‑100 (MQ=255)
   ttAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcgccg                                                                                              <  1:884391/100‑1 (MQ=255)
    tAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcgccgc                                                                                             >  2:3851673/1‑100 (MQ=255)
     aaCGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCa                                                                                            >  2:1595708/1‑100 (MQ=255)
       cGGTGCTGGTGATAGGTTATGCTCTCTGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATc                                                                                          <  2:664131/100‑1 (MQ=38)
        ggtggtggtGATAGGTTATGGTCTCGGCAAAATCGGGATGTTGTTGAGCTGACGATGTGTTTTTTGCGTCGATGCCGGATGCGGCGTAAACGCTGCATcc                                                                                         <  2:3157935/100‑1 (MQ=25)
        ggTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATcc                                                                                         <  2:2151881/100‑1 (MQ=38)
        ggTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATcc                                                                                         >  1:4064710/1‑100 (MQ=255)
         gTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCg                                                                                        <  1:3622450/100‑1 (MQ=255)
         gTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCg                                                                                        <  1:1830478/100‑1 (MQ=255)
         gTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCg                                                                                        <  1:3995384/100‑1 (MQ=255)
          tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                       <  2:2399143/100‑1 (MQ=38)
          tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                       <  1:1708812/100‑1 (MQ=38)
          tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                       <  2:1926432/100‑1 (MQ=38)
           gCTGTTGATAGGTAATACTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGCGGTCTGTGCGCCGATGCCGGATGCAGCGTTTACGCCGGATCCGGc                                                                                      <  2:1690685/100‑1 (MQ=255)
           gCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGc                                                                                      >  1:2767739/1‑100 (MQ=255)
           gCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGc                                                                                      <  2:939407/100‑1 (MQ=255)
            cTGGTGATGGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCGGCa                                                                                     <  2:1393560/100‑1 (MQ=37)
            cTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCa                                                                                     <  2:1863546/100‑1 (MQ=255)
             tGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAg                                                                                    <  1:3547577/100‑1 (MQ=255)
             tGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAg                                                                                    <  1:773321/100‑1 (MQ=255)
              ggTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGc                                                                                   <  2:4088917/100‑1 (MQ=255)
               gttagaGGTTAGGCTCTCTGCAAAAGCTCGATGTTATTTAGCTGACGATGTGTTATTTGCGCCTACGCCGTATGCAGCGTAAACCCTGCATCCGGc                                                                                      <  2:1169103/91‑1 (MQ=255)
               gTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGcc                                                                                  >  1:472267/1‑100 (MQ=255)
                tGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCg                                                                                 <  2:4134129/100‑1 (MQ=255)
                    aGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGCTGTAGCGTTTACGCCGCATCCGGCAGCCGTGtt                                                                             <  2:2716290/100‑1 (MQ=255)
                    aGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGtt                                                                             <  2:2892703/100‑1 (MQ=255)
                    aGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGtt                                                                             >  2:1013240/1‑100 (MQ=255)
                     ggTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCAGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGttt                                                                            >  1:2908586/1‑100 (MQ=255)
                       ttATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTAt                                                                          >  1:1840773/1‑100 (MQ=255)
                         aTGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATgg                                                                        <  1:3113272/100‑1 (MQ=255)
                          tGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATggg                                                                       <  1:1103556/100‑1 (MQ=255)
                          tGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATggg                                                                       >  1:3381725/1‑100 (MQ=255)
                          tGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATggg                                                                       <  2:427617/100‑1 (MQ=255)
                           gCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGa                                                                      <  2:288028/100‑1 (MQ=255)
                            ctctCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAt                                                                     <  1:1092879/100‑1 (MQ=255)
                            ctctCGGCAAAATCACGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGAGTGCAGCGTTTACACCGCATCCGGCAGCCTTGTTTATGGTAt                                                                     >  2:989738/1‑100 (MQ=255)
                                   cAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATTCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGACCTCAATg                                                              >  1:1320098/1‑100 (MQ=255)
                                   cAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATg                                                              <  2:799149/100‑1 (MQ=255)
                                   cAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCAGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATg                                                              >  1:3080411/1‑100 (MQ=255)
                                     aaaTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGc                                                            <  1:384509/100‑1 (MQ=255)
                                      aaTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCt                                                           <  1:2899224/100‑1 (MQ=255)
                                       aTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTa                                                          <  2:3682409/100‑1 (MQ=255)
                                          gcgATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAaat                                                       <  2:1540328/100‑1 (MQ=255)
                                             aTGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGAGGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAaataat                                                    <  2:95872/100‑1 (MQ=255)
                                               gttgttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcg                                                  <  1:2241379/100‑1 (MQ=255)
                                                ttgttTAGCTTACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCTGTATTTATGGGATCTCAATGGCTAAATAAtcgc                                                 <  2:2715506/100‑1 (MQ=255)
                                                 tgttTAGCTGACGATGTGTTTTTTGCGTCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgct                                                <  2:2459679/100‑1 (MQ=255)
                                                 tgttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgct                                                >  1:2957577/1‑100 (MQ=255)
                                                 tgttTAGCTGACGATGGGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgct                                                <  1:961919/100‑1 (MQ=255)
                                                  gttTAGCTGACGATGTGTTTTTTGCTCCGATTCCGGATGCAGAGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgctc                                               >  2:1958692/1‑100 (MQ=255)
                                                      aGCTGACGATGTGTTTTTTGCTGAAATGCCAGATGCAGCGTTTATGCCGGATCCGGCAGCCGTGTTTATGGGATTTCACTGGCTGaataa                                                     >  2:1082777/1‑90 (MQ=255)
                                                      aGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgccc                                           >  1:3705947/1‑100 (MQ=255)
                                                        cTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccgc                                         >  1:492892/1‑100 (MQ=255)
                                                            cGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCccgccgc                                      <  1:1012267/99‑1 (MQ=255)
                                                             gATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCccgccgccg                                    >  1:1420133/1‑100 (MQ=255)
                                                              aTGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGa                                   <  1:1284447/100‑1 (MQ=255)
                                                                  gtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACtt                               >  1:1014799/1‑100 (MQ=255)
                                                                  gtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACtt                               >  1:2392684/1‑100 (MQ=255)
                                                                    tttttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCa                             <  2:1074983/100‑1 (MQ=255)
                                                                     ttttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCAt                            <  2:619051/100‑1 (MQ=255)
                                                                      tttGCGGCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATc                           <  2:486512/100‑1 (MQ=255)
                                                                        ctcgcCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCa                         <  1:1640314/98‑1 (MQ=255)
                                                                          cgcCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCAcg                       >  2:2972113/1‑100 (MQ=255)
                                                                            ccGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTACATAATCGCTCGCCCGCCGCCGAACTTCATCCAcgc                      >  2:4046079/1‑99 (MQ=255)
                                                                            ccGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCAcgcg                     >  1:3902521/1‑100 (MQ=255)
                                                                            ccGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACATCATcc                          >  2:2592972/1‑95 (MQ=255)
                                                                             cGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCgg                    <  2:3637768/100‑1 (MQ=255)
                                                                              gATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCggg                   <  1:913961/100‑1 (MQ=255)
                                                                              gATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCggg                   <  1:1081139/100‑1 (MQ=255)
                                                                               aTGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGc                  <  1:1003441/100‑1 (MQ=255)
                                                                                tGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGcc                 >  1:1425197/1‑100 (MQ=255)
                                                                                 gCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCa                >  1:3634275/1‑100 (MQ=255)
                                                                                 gCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCa                <  1:1296436/100‑1 (MQ=255)
                                                                                  ccGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCat               >  1:3131924/1‑100 (MQ=38)
                                                                                  ccGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCACCGCCGAACTTCATCCACGCGGGCCat               >  2:748184/1‑100 (MQ=255)
                                                                                   cGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCata              >  1:1925008/1‑100 (MQ=38)
                                                                                   cGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCata              >  1:613085/1‑100 (MQ=38)
                                                                                    ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGcc                 >  1:843317/1‑96 (MQ=38)
                                                                                    ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat             >  1:1171060/1‑100 (MQ=38)
                                                                                    ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat             >  1:2924161/1‑100 (MQ=38)
                                                                                    ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCata              >  2:1689573/1‑99 (MQ=38)
                                                                                    ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACATCATCCACGCGGGCCatat             >  1:1927632/1‑100 (MQ=38)
                                                                                    ggATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAGCTTCATCCACGCGGGCCatat             >  1:940894/1‑100 (MQ=255)
                                                                                        gCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGtt         <  1:3413939/100‑1 (MQ=255)
                                                                                         cAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGttt        >  1:3881119/1‑100 (MQ=255)
                                                                                         cAGCGTGGACTCCGCATCCGGCAGCCGTGTTTATGTGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGttt        <  1:1787368/100‑1 (MQ=255)
                                                                                          aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGCACTTCATCCACGCGGGCCATATCGTTTg       >  1:879471/1‑100 (MQ=255)
                                                                                          aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg       >  1:1277381/1‑100 (MQ=255)
                                                                                          aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg       <  1:1207304/100‑1 (MQ=255)
                                                                                          aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg       <  2:3282284/100‑1 (MQ=255)
                                                                                            cGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGAc     <  1:998394/100‑1 (MQ=255)
                                                                                            cGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGAc     <  2:3017756/100‑1 (MQ=255)
                                                                                            cGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGAc     <  2:201901/100‑1 (MQ=255)
                                                                                             gTTTCCGCCGCATCCGGCAGCCGTGTGTATGGGCTCTCACTGGCGAAATAATCCCTAGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGtt         <  2:3373378/95‑1 (MQ=255)
                                                                                              tttACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTg   <  1:2519498/100‑1 (MQ=255)
                                                                                               ttACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGc  <  2:3454803/100‑1 (MQ=255)
                                                                                               |                                                                                                   
AGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGC  >  NC_000913/681604‑681798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: