Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 681,705 C→T 17.1% intergenic (+42/+18) djlC → / ← hscC J domain‑containing HscC co‑chaperone; Hsc56/Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913681,7050CT17.1% 346.5 / 56.2 151intergenic (+42/+18)djlC/hscCJ domain‑containing HscC co‑chaperone; Hsc56/Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor
Reads supporting (aligned to +/- strand):  ref base C (50/75);  new base T (15/11);  total (65/86)
Fisher's exact test for biased strand distribution p-value = 1.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.02e-01

Only 100 of 157 total aligned reads displayed.

TTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCC  >  NC_000913/681607‑681804
                                                                                                  |                                                                                                   
 tAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcgccgc                                                                                                   >  2:3851673/1‑100 (MQ=255)
  aaCGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCa                                                                                                  >  2:1595708/1‑100 (MQ=255)
    cGGTGCTGGTGATAGGTTATGCTCTCTGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATc                                                                                                <  2:664131/100‑1 (MQ=38)
    cGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATc                                                                                                <  1:181209/100‑1 (MQ=38)
     ggtggtggtGATAGGTTATGGTCTCGGCAAAATCGGGATGTTGTTGAGCTGACGATGTGTTTTTTGCGTCGATGCCGGATGCGGCGTAAACGCTGCATcc                                                                                               <  2:3157935/100‑1 (MQ=25)
     ggTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATcc                                                                                               <  2:2151881/100‑1 (MQ=38)
      gTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCg                                                                                              <  1:3995384/100‑1 (MQ=255)
      gTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCg                                                                                              <  1:1830478/100‑1 (MQ=255)
       tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                             <  2:1926432/100‑1 (MQ=38)
       tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                             <  1:1708812/100‑1 (MQ=38)
       tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCgg                                                                                             <  1:374139/100‑1 (MQ=255)
        gCTGTTGATAGGTAATACTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGCGGTCTGTGCGCCGATGCCGGATGCAGCGTTTACGCCGGATCCGGc                                                                                            <  2:1690685/100‑1 (MQ=255)
        gCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGc                                                                                            >  1:2767739/1‑100 (MQ=255)
        gCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGc                                                                                            <  2:939407/100‑1 (MQ=255)
         cTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCa                                                                                           <  2:1863546/100‑1 (MQ=255)
          tGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAg                                                                                          >  2:2390765/1‑100 (MQ=255)
          tGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAg                                                                                          >  1:1581837/1‑100 (MQ=255)
          tGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAg                                                                                          <  1:773321/100‑1 (MQ=255)
          tGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAg                                                                                          <  1:3547577/100‑1 (MQ=255)
           ggTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGc                                                                                         <  2:4088917/100‑1 (MQ=255)
            gttagaGGTTAGGCTCTCTGCAAAAGCTCGATGTTATTTAGCTGACGATGTGTTATTTGCGCCTACGCCGTATGCAGCGTAAACCCTGCATCCGGc                                                                                            <  2:1169103/91‑1 (MQ=255)
            gTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGcc                                                                                        >  1:472267/1‑100 (MQ=255)
            gTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGcc                                                                                        >  1:2865193/1‑100 (MQ=255)
             tGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCg                                                                                       <  2:4134129/100‑1 (MQ=255)
             tGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCg                                                                                       <  2:1138566/100‑1 (MQ=255)
                 aGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGCTGTAGCGTTTACGCCGCATCCGGCAGCCGTGtt                                                                                   <  2:2716290/100‑1 (MQ=255)
                 aGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGtt                                                                                   >  2:1013240/1‑100 (MQ=255)
                 aGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGtt                                                                                   <  2:2892703/100‑1 (MQ=255)
                  ggTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGttt                                                                                  >  1:427620/1‑100 (MQ=255)
                   gTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTa                                                                                 >  1:1376653/1‑100 (MQ=255)
                      aTGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATgg                                                                              <  1:3113272/100‑1 (MQ=255)
                       tGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATggg                                                                             <  2:427617/100‑1 (MQ=255)
                       tGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATggg                                                                             <  1:1103556/100‑1 (MQ=255)
                       tGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATggg                                                                             >  1:3381725/1‑100 (MQ=255)
                         ctctCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAt                                                                           <  1:1092879/100‑1 (MQ=255)
                         ctctCGGCAAAATCACGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGAGTGCAGCGTTTACACCGCATCCGGCAGCCTTGTTTATGGTAt                                                                           >  2:989738/1‑100 (MQ=255)
                                cAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATTCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGACCTCAATg                                                                    >  1:1320098/1‑100 (MQ=255)
                                cAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATg                                                                    <  2:799149/100‑1 (MQ=255)
                                  aaaTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGc                                                                  <  1:384509/100‑1 (MQ=255)
                                   aaTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCt                                                                 <  1:2899224/100‑1 (MQ=255)
                                    aTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTa                                                                <  2:3682409/100‑1 (MQ=255)
                                       gcgATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAaat                                                             <  2:1537699/100‑1 (MQ=255)
                                       gcgATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAaat                                                             <  2:1540328/100‑1 (MQ=255)
                                          aTGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCTGCAGCCGTGTTTATGGGATCTCAATGGCTAaataat                                                          <  1:2920506/100‑1 (MQ=255)
                                          aTGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGAGGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAaataat                                                          <  2:95872/100‑1 (MQ=255)
                                            gttgttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcg                                                        <  2:2686644/100‑1 (MQ=255)
                                            gttgttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcg                                                        <  1:94592/100‑1 (MQ=255)
                                            gttgttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcg                                                        <  1:2241379/100‑1 (MQ=255)
                                              tgttTAGCTGACGATGTGTTTTTTGCGTCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgct                                                      <  2:2459679/100‑1 (MQ=255)
                                              tgttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgct                                                      >  1:2957577/1‑100 (MQ=255)
                                              tgttTAGCTGACGATGGGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgct                                                      <  1:961919/100‑1 (MQ=255)
                                               gttTAGCTGACGATGTGTTTTTTGCTCCGATTCCGGATGCAGAGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgctc                                                     >  2:1958692/1‑100 (MQ=255)
                                                  tAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCTGCATCCGGCAGCCGCGTTTATGGGATCTCAACGGCTAAATAAtcgctcgc                                                   >  2:3400715/1‑99 (MQ=255)
                                                     cTGACGATGCGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGACGTGTTTATAGGATCTCAATGGCTAAATAAtcgct                                                      >  2:2796422/1‑93 (MQ=255)
                                                      tGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccgcc                                              <  1:1291280/100‑1 (MQ=255)
                                                         cGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCccgccgc                                            <  1:1012267/99‑1 (MQ=255)
                                                          gATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCTCTCGCccgccgccg                                          >  1:1765375/1‑100 (MQ=255)
                                                           aTGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGa                                         <  1:1284447/100‑1 (MQ=255)
                                                           aTGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGa                                         <  1:2434671/100‑1 (MQ=255)
                                                             gtgtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAAc                                       >  1:3629845/1‑100 (MQ=255)
                                                               gtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACtt                                     >  1:1014799/1‑100 (MQ=255)
                                                                 tttttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCa                                   <  2:1074983/100‑1 (MQ=255)
                                                                  ttttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCAt                                  <  2:619051/100‑1 (MQ=255)
                                                                   tttGCGGCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATc                                 <  2:486512/100‑1 (MQ=255)
                                                                       cgcCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCaag                             >  2:1640302/1‑98 (MQ=255)
                                                                       cgcCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCAcg                             >  2:2972113/1‑100 (MQ=255)
                                                                       cgcCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCAcg                             >  1:3060249/1‑100 (MQ=255)
                                                                         ccGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTACATAATCGCTCGCCCGCCGCCGAACTTCATCCAcgc                            >  2:4046079/1‑99 (MQ=255)
                                                                         ccGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCAcgcg                           >  1:3902521/1‑100 (MQ=255)
                                                                           gATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCggg                         <  1:1081139/100‑1 (MQ=255)
                                                                           gATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCggg                         <  1:913961/100‑1 (MQ=255)
                                                                            aTGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGc                        <  1:1003441/100‑1 (MQ=255)
                                                                             tGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGcc                       >  1:1425197/1‑100 (MQ=255)
                                                                              gCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCa                      >  1:3634275/1‑100 (MQ=255)
                                                                               ccGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCat                     >  1:1254775/1‑100 (MQ=255)
                                                                               ccGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCACCGCCGAACTTCATCCACGCGGGCCat                     >  2:748184/1‑100 (MQ=255)
                                                                                cGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCata                    >  1:613085/1‑100 (MQ=38)
                                                                                cGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCata                    >  1:1925008/1‑100 (MQ=38)
                                                                                cGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCata                    >  1:3096709/1‑100 (MQ=38)
                                                                                 ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGcc                       >  1:843317/1‑96 (MQ=38)
                                                                                 ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat                   >  1:1171060/1‑100 (MQ=38)
                                                                                 ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat                   >  1:2658191/1‑100 (MQ=38)
                                                                                      cggcgtaaaCGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGttt              >  1:1782624/9‑100 (MQ=255)
                                                                                      cggcgtaaaCGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGttt              >  2:1877048/9‑100 (MQ=255)
                                                                                      cAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGttt              >  1:3881119/1‑100 (MQ=255)
                                                                                      cAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGttt              <  1:3191692/100‑1 (MQ=255)
                                                                                      cAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGttt              <  1:52746/100‑1 (MQ=255)
                                                                                       aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGCACTTCATCCACGCGGGCCATATCGTTTg             >  1:879471/1‑100 (MQ=255)
                                                                                       aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg             <  1:1885332/100‑1 (MQ=255)
                                                                                       aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg             >  1:886186/1‑100 (MQ=255)
                                                                                       aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg             <  2:3282284/100‑1 (MQ=255)
                                                                                       aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg             <  1:1207304/100‑1 (MQ=255)
                                                                                         cGTTTACGTCGCATTCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAGCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGAc           <  2:1009426/100‑1 (MQ=255)
                                                                                         cGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGAc           <  2:201901/100‑1 (MQ=255)
                                                                                         cGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGAc           <  2:3017756/100‑1 (MQ=255)
                                                                                          gTTTCCGCCGCATCCGGCAGCCGTGTGTATGGGCTCTCACTGGCGAAATAATCCCTAGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGtt               <  2:3373378/95‑1 (MQ=255)
                                                                                           tttACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTg         <  1:2519498/100‑1 (MQ=255)
                                                                                            ttACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGc        <  2:3454803/100‑1 (MQ=255)
                                                                                            ttACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGc        <  2:2545171/100‑1 (MQ=255)
                                                                                               cgccgcATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAAc     >  2:2700565/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
TTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCC  >  NC_000913/681607‑681804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: