Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 772,209 C→A 100% T251N (ACC→AAC)  cydA → cytochrome d terminal oxidase, subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913772,2090CA97.8% 453.2 / ‑0.6 136T251N (ACC→AAC) cydAcytochrome d terminal oxidase, subunit I
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (67/65);  total (68/68)
Fisher's exact test for biased strand distribution p-value = 6.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 138 total aligned reads displayed.

TCCTTTGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAACCAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACGAACAA  >  NC_000913/772112‑772305
                                                                                                 |                                                                                                
tCCTTTGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACa                                                                                                <  2:1452197/100‑1 (MQ=255)
tCCTTTGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACa                                                                                                <  2:1908169/100‑1 (MQ=255)
tCCTTTGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTATGGGTGATGAATTCGGCTACGAAATGGGCGACGTGCAGAAAAACa                                                                                                >  1:782983/1‑100 (MQ=255)
 ccTTTGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGTTACGAAATGGGCGACGTGCAGAAAAACaa                                                                                               >  2:2968430/1‑100 (MQ=255)
 ccTTTGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACaa                                                                                               <  1:6626/100‑1 (MQ=255)
 ccTTTGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACaa                                                                                               <  1:2471571/100‑1 (MQ=255)
  ccttacTATCGCTGCCAGCTTCGGTATGGCGGCTGTTCTGTCTGTTATTGTGCTGGGTGATGAATCCCGCTACGACATGGGCGACGTGCAGAAAAACa                                                                                                <  2:2620822/93‑1 (MQ=255)
   tttGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAaaacaaac                                                                                             >  1:153930/1‑100 (MQ=255)
    ttGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACt                                                                                            <  1:2570616/100‑1 (MQ=255)
      gCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTgg                                                                                          >  1:3709651/1‑100 (MQ=255)
       cTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGgc                                                                                         >  2:2298318/1‑100 (MQ=255)
        tATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGgct                                                                                        <  1:326230/100‑1 (MQ=255)
           cGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGgctgct                                                                                     <  1:3379842/100‑1 (MQ=255)
            gCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTa                                                                                    <  1:2117544/100‑1 (MQ=255)
            gCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTa                                                                                    >  1:2185343/1‑100 (MQ=255)
             cTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTAt                                                                                   >  1:266931/1‑100 (MQ=255)
             cTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTAt                                                                                   <  2:2604142/100‑1 (MQ=255)
              tGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTAtt                                                                                  <  1:3316465/100‑1 (MQ=255)
               gCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTg                                                                                 >  1:3060454/1‑100 (MQ=255)
                 cAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGaa                                                                               >  2:3426207/1‑100 (MQ=255)
                 cAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGaa                                                                               >  2:102027/1‑100 (MQ=255)
                  aGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAg                                                                              <  2:2920970/100‑1 (MQ=255)
                   gCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTAATGAAg                                                                              >  2:3916223/1‑99 (MQ=255)
                     ttCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCg                                                                           <  1:2103124/100‑1 (MQ=255)
                       cGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAg                                                                         >  1:3557767/1‑100 (MQ=255)
                         gTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTg                                                                       <  1:2316310/100‑1 (MQ=255)
                         gTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTg                                                                       <  1:2392228/100‑1 (MQ=255)
                         gTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTg                                                                       >  2:1334268/1‑100 (MQ=255)
                          tATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTgg                                                                      <  1:2470057/100‑1 (MQ=255)
                          tATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTgg                                                                      <  1:3745263/100‑1 (MQ=255)
                          tATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTgg                                                                      <  1:1585753/100‑1 (MQ=255)
                          tATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGAGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTgg                                                                      >  2:2900607/1‑100 (MQ=255)
                           aTGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTggg                                                                     <  1:1057932/100‑1 (MQ=255)
                            tGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCAACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTggg                                                                     <  2:763781/99‑1 (MQ=255)
                                tgctgTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACg                                                                >  2:1606821/1‑100 (MQ=255)
                                  ctgTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCa                                                              >  1:3218290/1‑100 (MQ=255)
                                    gTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAAc                                                            >  1:3384261/1‑100 (MQ=255)
                                    gTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAAc                                                            >  1:3840785/1‑100 (MQ=255)
                                    gTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAAc                                                            <  1:456777/100‑1 (MQ=255)
                                      tctgtctgTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAAcc                                                           >  2:3123530/1‑99 (MQ=255)
                                        tgtctgtTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTgc                                                        >  1:845826/1‑100 (MQ=255)
                                            tggtATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGGGTGGGAAACGCAACCTGCGcc                                                     <  2:2730504/96‑1 (MQ=255)
                                            ngtTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCct                                                    >  2:2369541/2‑100 (MQ=255)
                                               tATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCctgct                                                 <  1:3342161/100‑1 (MQ=255)
                                               tATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCctgct                                                 <  2:422636/100‑1 (MQ=255)
                                               tATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCctgct                                                 <  1:1676731/100‑1 (MQ=255)
                                                aTTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCctgctg                                                >  1:2295305/1‑100 (MQ=255)
                                                  tGTTCTGGGTGATGAATCCGGTTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGcc                                              <  1:2968447/100‑1 (MQ=255)
                                                  tGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCtgctgc                                               >  2:802707/1‑99 (MQ=255)
                                                  tGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGcc                                              <  2:3806897/100‑1 (MQ=255)
                                                  tGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGcc                                              >  2:3221/1‑100 (MQ=255)
                                                  tGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGcc                                              <  2:2911592/100‑1 (MQ=255)
                                                   gTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCt                                             >  2:3206739/1‑100 (MQ=255)
                                                   gTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCt                                             >  2:1181216/1‑100 (MQ=255)
                                                      cTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTa                                          >  1:345552/1‑100 (MQ=255)
                                                       tGGGTGAAGAATCCGGCTAGGAAATCGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTAc                                         <  2:1026093/100‑1 (MQ=255)
                                                        gggTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTAct                                        >  2:807546/1‑100 (MQ=255)
                                                        gggTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTAct                                        >  1:984101/1‑100 (MQ=255)
                                                        gggTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTAct                                        >  1:1886564/1‑100 (MQ=255)
                                                         ggTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACACACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTActc                                       >  2:1187551/1‑100 (MQ=255)
                                                          gTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTAACTTTActct                                      >  2:313110/1‑100 (MQ=255)
                                                            gatgaATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGt                                    >  2:3776984/1‑100 (MQ=255)
                                                             atgaATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGtt                                   <  1:1699470/100‑1 (MQ=255)
                                                             atgaATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGtt                                   <  1:1987270/100‑1 (MQ=255)
                                                             atgaATCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGtt                                   <  2:2946239/100‑1 (MQ=255)
                                                                aaTCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCgg                                <  2:866612/100‑1 (MQ=255)
                                                                aaTCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCgg                                <  1:1463274/100‑1 (MQ=255)
                                                                aaTCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCgg                                >  2:2209300/1‑100 (MQ=255)
                                                                 aTCCGGCTACGAAATGGGCGACGTGCAGAAAACCAACCTGGCTGCTATTGAAGCCGAGTGGTAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGc                               <  2:2144940/100‑1 (MQ=255)
                                                                 aTCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGc                               >  1:1540537/1‑100 (MQ=255)
                                                                 aTCCGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGc                               <  1:1606833/100‑1 (MQ=255)
                                                                 aTCCGGCTACGAAAGGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGc                               <  2:2668291/100‑1 (MQ=255)
                                                                   ccGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCAt                             >  2:4079990/1‑100 (MQ=255)
                                                                    gatcTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCAtt                            <  2:1553102/97‑1 (MQ=255)
                                                                    cGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCAtt                            <  1:2232297/100‑1 (MQ=255)
                                                                    cGGCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCAtt                            >  2:1355626/1‑100 (MQ=255)
                                                                      gCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCCACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTcc                          <  1:2911549/100‑1 (MQ=255)
                                                                      gCTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTcc                          <  1:2139816/100‑1 (MQ=255)
                                                                       cTACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCt                         >  1:804302/1‑100 (MQ=255)
                                                                       cTACGAAATGAGCGACGTTCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCt                         >  2:3449862/1‑100 (MQ=255)
                                                                        tACGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTg                        >  1:396666/1‑100 (MQ=255)
                                                                          cGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGAt                      >  2:1905834/1‑100 (MQ=255)
                                                                          cGAAATGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGAt                      <  2:1167447/100‑1 (MQ=255)
                                                                           gAAATGGGCGACTTGCAGAAAAACAAACTGGCTGCTATTGAATCTGAGTGGGAAACGCAACCTGCGCCTTCTGCCTTTATTCTGTTCGGCATTCCTGATc                     <  2:3277569/100‑1 (MQ=255)
                                                                            aaaTGGGCGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCAGTCCTGAt                      <  1:1556163/98‑1 (MQ=255)
                                                                                 ggCGACGTGCAGAAAAACAAATTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAag               >  2:475874/1‑100 (MQ=255)
                                                                                   cGACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAagag             <  1:2900623/100‑1 (MQ=255)
                                                                                    gACGTGCAGAAAATCAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAgagg            <  1:1925097/100‑1 (MQ=255)
                                                                                    gACGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAgagg            <  1:1114660/100‑1 (MQ=255)
                                                                                      cGTGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAgaggag          >  1:2545444/1‑100 (MQ=255)
                                                                                        tGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGAc        <  2:2803559/100‑1 (MQ=255)
                                                                                        tGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGAc        >  2:1658567/1‑100 (MQ=255)
                                                                                        tGCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGAc        >  2:3875383/1‑100 (MQ=255)
                                                                                         gCAGAAAACCAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACg       <  1:1941099/100‑1 (MQ=255)
                                                                                         gCAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACg       >  2:231174/1‑100 (MQ=255)
                                                                                          cAGAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACGa      >  1:2073877/1‑100 (MQ=255)
                                                                                           aGAAAAACAAACTGGCTGCTATAGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACGaa     <  1:1008958/100‑1 (MQ=255)
                                                                                            gAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACGAAc    >  2:693712/1‑100 (MQ=255)
                                                                                            gAAAAACAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACGAAc    >  2:3064942/1‑100 (MQ=255)
                                                                                              aaaaCAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACGAACaa  >  2:3848772/1‑100 (MQ=255)
                                                                                                 |                                                                                                
TCCTTTGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGAAATGGGCGACGTGCAGAAAACCAAACTGGCTGCTATTGAAGCCGAGTGGGAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAGACGAACAA  >  NC_000913/772112‑772305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: