Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 162,973 G→A 100% C290Y (TGT→TAT)  hrpB → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913162,9730GA98.7% 494.3 / ‑3.0 153C290Y (TGT→TAT) hrpBputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (72/78);  total (73/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01

Only 100 of 155 total aligned reads displayed.

ATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGG  >  NC_000913/162876‑163069
                                                                                                 |                                                                                                
aTCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATtatg                                                                                                <  1:2570961/100‑1 (MQ=255)
 tcctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGc                                                                                               <  2:3572147/100‑1 (MQ=255)
 tcctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGc                                                                                               <  2:1808378/100‑1 (MQ=255)
 tcctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGc                                                                                               <  1:4095794/100‑1 (MQ=255)
  cctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGcc                                                                                              >  2:910659/1‑100 (MQ=255)
   ctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGccc                                                                                             <  1:2069579/100‑1 (MQ=255)
     cccGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAg                                                                                           >  1:3570320/1‑100 (MQ=255)
      ccGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGccc                                                                                             >  2:1496914/1‑97 (MQ=255)
      ccGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAgg                                                                                          <  2:515985/100‑1 (MQ=255)
      ccGGCACCGCAAGGGATGCGCAAAGTGGTGCGGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGccc                                                                                             <  2:1802636/97‑1 (MQ=255)
        ggCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAg                                                                                        <  2:124433/100‑1 (MQ=255)
          cACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCg                                                                                      <  2:2429503/100‑1 (MQ=255)
          cACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCg                                                                                      <  1:367011/100‑1 (MQ=255)
           aacGCAAGGGATGCGCAAAGTGGTTCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCg                                                                                      <  2:1458381/97‑1 (MQ=255)
            ccGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCgtg                                                                                    >  2:1046863/1‑100 (MQ=255)
            ccGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCgtg                                                                                    >  1:354384/1‑100 (MQ=255)
              gCAAGGGATGCGCAAAGAGGGACCGGCAACCAATAATGGTGACAACAGTTTAACCATGGAAGGAACGCGTCTGGTAGTGGATAATGCCAAg                                                                                           >  2:2657241/1‑91 (MQ=255)
               cAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTgg                                                                                 <  1:642374/100‑1 (MQ=255)
               cAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACAAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTgg                                                                                 >  2:3234323/1‑100 (MQ=255)
                aaGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGgc                                                                                <  1:2712253/100‑1 (MQ=255)
                aaGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGgc                                                                                <  1:2205921/100‑1 (MQ=255)
                 aGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGgcg                                                                               <  2:1294407/100‑1 (MQ=255)
                   ggATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGcgcg                                                                             <  1:2451533/100‑1 (MQ=255)
                     aTGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtt                                                                           <  1:3129529/100‑1 (MQ=255)
                      tGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGttt                                                                          >  1:2884391/1‑100 (MQ=255)
                       gcgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtttt                                                                         <  2:3650519/100‑1 (MQ=255)
                       gcgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtttt                                                                         <  2:368879/100‑1 (MQ=255)
                       gcgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtttt                                                                         >  2:1744770/1‑100 (MQ=255)
                        cgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTg                                                                        >  1:3401288/1‑100 (MQ=255)
                         gcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGa                                                                       >  1:808280/1‑100 (MQ=255)
                         gcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGa                                                                       >  2:2354572/1‑100 (MQ=255)
                         gcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGa                                                                       <  1:2018088/100‑1 (MQ=255)
                          cAAAGTGGTGCTGGCGACCAATTTTGCTGAAACCAGTTTAACCATTGCCGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGCGTGGCGCGTTTTGa                                                                       <  2:2810107/99‑1 (MQ=255)
                          cAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGAt                                                                      <  2:2273581/100‑1 (MQ=255)
                          cAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGAt                                                                      <  2:133478/100‑1 (MQ=255)
                             aGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCcg                                                                   <  2:2826806/100‑1 (MQ=255)
                               tggtgCTGGCGACCAATATTGCTGAGACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGTCGCGTTTTGATCcgcg                                                                 <  2:1993819/100‑1 (MQ=255)
                                   gCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACg                                                             >  1:781844/1‑100 (MQ=255)
                                    cTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACgg                                                            >  2:819311/1‑100 (MQ=255)
                                     tGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACggg                                                           >  1:3041009/1‑100 (MQ=255)
                                      ggCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACgggg                                                          >  1:102287/1‑100 (MQ=255)
                                      ggCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACggcg                                                          >  2:3780546/1‑98 (MQ=255)
                                       gCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGc                                                         <  1:3047210/100‑1 (MQ=255)
                                       gCGACCAATATTGCTGAAACCAGTTTAACCATGGAGGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGc                                                         <  2:3748788/100‑1 (MQ=255)
                                       gCGACCAATATTGCTGAAACCAGTGTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGc                                                         <  1:2456085/100‑1 (MQ=255)
                                         gACCAGTAGTGCTGAAACCAGTTTAACCATTGGAGGTATTCGTCTGGGGGGGGATTATGCACAGTAGTGTGTATCGCGTGGTGAGCCGCGCAc                                                              >  2:2462684/1‑93 (MQ=255)
                                         gACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCtt                                                       >  1:2276225/1‑100 (MQ=255)
                                           ccAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTTATCCGCGCACCGGGCTTAc                                                     >  2:2345054/1‑100 (MQ=255)
                                              atatTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcgcg                                                  <  1:833296/100‑1 (MQ=255)
                                              atatTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcgcg                                                  >  2:3192555/1‑100 (MQ=255)
                                                atTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGAc                                                <  2:2861104/100‑1 (MQ=255)
                                                 ttGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACt                                               <  1:2619215/100‑1 (MQ=255)
                                                 tggCTGAAACCAGTTGAACCCTGGTAGGTATTCGTCTGGTGGTGGATTATGCCCACGGGCGTGTGGCGCGTTTTGATCCGCGGACGGGGCTTACGCGACt                                               <  2:1409505/98‑1 (MQ=255)
                                                  tGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGCTCCGCGCACGGGGCTTACGCGACTg                                              >  2:3313131/1‑100 (MQ=255)
                                                  tGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTg                                              >  2:4056784/1‑100 (MQ=255)
                                                  tGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTg                                              <  1:2960128/100‑1 (MQ=255)
                                                  tGCTGAAAACAGTTTAACCATTGCAGGTATTCGTCGGGTTGTGGATTATGCCCGGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGAc                                                <  2:2725246/98‑1 (MQ=255)
                                                   gCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGa                                             >  1:3463386/1‑100 (MQ=255)
                                                    cTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGAt                                            >  2:3277330/1‑100 (MQ=255)
                                                    cTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGAt                                            >  1:3625180/1‑100 (MQ=255)
                                                     tGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGAtt                                           <  2:3527398/100‑1 (MQ=255)
                                                      gAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTa                                          >  2:2963668/1‑100 (MQ=255)
                                                       aaaCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTAc                                         >  2:1269076/1‑100 (MQ=255)
                                                        aaCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGAGTGTGGCGCGTTTTGGTCTGCGCACGGGGCTTACGGGACTGATTACt                                        >  2:407303/1‑100 (MQ=255)
                                                           cAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTATGCGACTGATTACTCaa                                     >  1:1589125/1‑100 (MQ=255)
                                                             gTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcg                                   >  1:2691008/1‑100 (MQ=255)
                                                              tttAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcg                                   <  2:3869461/99‑1 (MQ=255)
                                                               ttAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcgcg                                 <  1:452947/100‑1 (MQ=255)
                                                                tAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGt                                <  1:1232706/100‑1 (MQ=255)
                                                                tAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGt                                <  1:506976/100‑1 (MQ=255)
                                                                 aaCCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGtt                               <  1:1381641/100‑1 (MQ=255)
                                                                   ccATTGAAGGTATTCGTCTGGTGGTGGATTATGCCGAGGAGCGTGTGGCGCGTTTTGATCCGCGAACGGGGCTTACGCGACTGATTACTCAACGCGTTAg                             <  2:3930879/100‑1 (MQ=255)
                                                                   ccATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAg                             <  1:179846/100‑1 (MQ=255)
                                                                    cATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGc                            <  1:160743/100‑1 (MQ=255)
                                                                       tGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAg                         >  1:1978995/1‑100 (MQ=255)
                                                                        gAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAgg                        >  2:2510981/1‑100 (MQ=255)
                                                                        gAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAgg                        >  2:1486565/1‑100 (MQ=255)
                                                                           ggTATTCGTCTGGTGGAGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATACGCGCACGGGGCTTAAGCGACTGATTACTCAATGCGTTAGCCAGGCAt                     >  2:714161/1‑100 (MQ=255)
                                                                             tATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATcc                   <  1:3965769/100‑1 (MQ=255)
                                                                             tATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATcc                   >  1:387713/1‑100 (MQ=255)
                                                                              aTTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATccn                  <  2:3890845/100‑2 (MQ=255)
                                                                               ttCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCAt                 <  2:555976/100‑1 (MQ=255)
                                                                                 cGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGa               >  1:1776877/1‑100 (MQ=255)
                                                                                 cGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGa               <  2:887264/100‑1 (MQ=255)
                                                                                 cGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGa               >  1:251262/1‑100 (MQ=255)
                                                                                  gTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGAc              <  1:3815877/100‑1 (MQ=255)
                                                                                  gTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGAc              <  1:14142/100‑1 (MQ=255)
                                                                                   tCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACg             <  1:2077263/100‑1 (MQ=255)
                                                                                    cTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGc            >  2:1610872/1‑100 (MQ=255)
                                                                                     tggtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCa           >  1:3402817/1‑100 (MQ=255)
                                                                                      ggtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGctg          <  1:2784731/100‑3 (MQ=255)
                                                                                      ggtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGAATGATTACTCAACGCGTTAGCCAGGCATCCATGACGCa           <  2:2198760/99‑1 (MQ=255)
                                                                                       gtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGc         >  1:1777856/1‑100 (MQ=255)
                                                                                        tggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCg        >  2:747568/1‑100 (MQ=255)
                                                                                        tggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCg        <  2:2805073/100‑1 (MQ=255)
                                                                                        tggtggAGTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCg        <  1:3889320/100‑1 (MQ=255)
                                                                                         ggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGt       >  1:1000746/1‑100 (MQ=255)
                                                                                          gtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTg      >  1:3683861/1‑100 (MQ=255)
                                                                                            ggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGcc    >  1:1289625/1‑100 (MQ=255)
                                                                                              aTTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCgg  <  1:2535820/100‑1 (MQ=255)
                                                                                                 |                                                                                                
ATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGG  >  NC_000913/162876‑163069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: