Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 849,405 G→A 54.8% T231T (ACC→ACT)  rhtA ← threonine and homoserine efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913849,4050GA54.8% 34.8 / 201.7 156T231T (ACC→ACT) rhtAthreonine and homoserine efflux system
Reads supporting (aligned to +/- strand):  ref base G (28/42);  new base A (37/48);  total (65/91)
Fisher's exact test for biased strand distribution p-value = 7.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 158 total aligned reads displayed.

TATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGACCAGTGCCAGAGTGCTTC  >  NC_000913/849306‑849500
                                                                                                   |                                                                                               
tATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACgg                                                                                                 <  1:6266/100‑1 (MQ=255)
  tGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGt                                                                                               <  1:928924/100‑1 (MQ=255)
  tGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGt                                                                                               <  1:729338/100‑1 (MQ=255)
   gggTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTg                                                                                              <  2:1192047/100‑1 (MQ=255)
   gggTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTg                                                                                              <  1:176879/100‑1 (MQ=255)
     gtgtCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTg                                                                                              <  2:1256074/98‑1 (MQ=255)
     gtgtCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAg                                                                                            >  2:3714614/1‑100 (MQ=255)
       gtCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAggg                                                                                          <  1:1100553/100‑1 (MQ=255)
       gtCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAggg                                                                                          <  1:3958627/100‑1 (MQ=255)
        tCAGGGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGc                                                                                         <  2:820352/100‑1 (MQ=255)
         cAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCa                                                                                        >  1:3698665/1‑100 (MQ=255)
           gtgtTTCTCCGAGGAAATTCATCCCGGAAACTGCAGCCAGCGGCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTTTTGGCAAACGGGTGAGGGCAAt                                                                                      <  2:755451/100‑1 (MQ=255)
            tgtTTCTCCGAGGAAAATCATCCCGGAAGCGGCAGCCAGCGCCTGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATc                                                                                     <  1:257746/100‑1 (MQ=255)
             gtTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATc                                                                                     >  2:1987636/1‑99 (MQ=255)
             gtTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCa                                                                                    >  2:1199968/1‑100 (MQ=255)
             gtTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCa                                                                                    <  1:1704084/100‑1 (MQ=255)
                tctcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCAttt                                                                                 <  1:3644127/100‑1 (MQ=255)
                tctcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCAttt                                                                                 >  1:749202/1‑100 (MQ=255)
                 ctcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTc                                                                                >  1:3139620/1‑100 (MQ=255)
                 ctcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTc                                                                                >  2:1437492/1‑100 (MQ=255)
                  tcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTcc                                                                               <  1:2495348/100‑1 (MQ=255)
                   ccGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCa                                                                              >  1:2993927/1‑100 (MQ=255)
                   ccGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCa                                                                              >  1:3924771/1‑100 (MQ=255)
                    cGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAg                                                                             >  1:199807/1‑100 (MQ=255)
                    cGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAg                                                                             >  1:2267576/1‑100 (MQ=255)
                      aGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg                                                                           <  1:1188879/100‑1 (MQ=255)
                       ggAAAATCATCCCGGCAACGGCAGCCACCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTCAGGGCAATCATTTCCCGc                                                                            >  2:579835/1‑98 (MQ=255)
                       ggAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGa                                                                          >  1:3073591/1‑100 (MQ=255)
                        gAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGaa                                                                         >  2:3667130/1‑100 (MQ=255)
                         aaaaTCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAAt                                                                        <  1:2487427/100‑1 (MQ=255)
                         aaaaTCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAAt                                                                        <  1:345115/100‑1 (MQ=255)
                          aaaTCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATa                                                                       <  2:2945765/100‑1 (MQ=255)
                            atcatcCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATaan                                                                     <  1:4079732/100‑2 (MQ=255)
                              catcCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGc                                                                   <  1:357141/100‑1 (MQ=255)
                               atcCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCa                                                                  >  1:249366/1‑100 (MQ=255)
                               atcCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCa                                                                  >  2:44535/1‑100 (MQ=255)
                               atcCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCa                                                                  >  1:3588704/1‑100 (MQ=255)
                                tcCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCag                                                                 >  2:4016773/1‑100 (MQ=255)
                                 cccGGAAACGGCAGCCAGCGCGGGTGCCATGCTCAGGATCGGACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGc                                                                   <  2:4031615/97‑1 (MQ=255)
                                 cccGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCaga                                                                <  2:2971096/100‑1 (MQ=255)
                                 cccGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCaga                                                                >  1:1453863/1‑100 (MQ=255)
                                 cccGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCaga                                                                >  2:614404/1‑100 (MQ=255)
                                  ccGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCagag                                                               >  1:3549159/1‑100 (MQ=255)
                                  ccGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGCGAGGGCAATCATGTCCAGCGAATAAGGCagag                                                               >  2:3219745/1‑100 (MQ=255)
                                      aaaCGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGt                                                           <  2:2572659/100‑1 (MQ=255)
                                      aaaCGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGt                                                           >  2:3909379/1‑100 (MQ=255)
                                        aCGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCg                                                         <  1:252592/100‑1 (MQ=255)
                                         cGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCga                                                        <  2:3210940/100‑1 (MQ=255)
                                         cGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCga                                                        <  1:959601/100‑1 (MQ=255)
                                          ggCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCgag                                                       <  1:372540/100‑1 (MQ=255)
                                          ggCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCgag                                                       <  1:2999086/100‑1 (MQ=255)
                                          ggCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCgag                                                       <  2:3549136/100‑1 (MQ=255)
                                          ggCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCgag                                                       >  2:3020140/1‑100 (MQ=255)
                                          ggCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCgag                                                       <  1:1781913/100‑1 (MQ=255)
                                           gCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCgaga                                                      >  1:477268/1‑100 (MQ=255)
                                           gCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCgaga                                                      <  1:1318614/100‑1 (MQ=255)
                                            cagccagcGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCgagag                                                     <  2:2373514/100‑1 (MQ=255)
                                              gccagcGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGaa                                                   >  1:537703/1‑100 (MQ=255)
                                              gccagcGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGAGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGaa                                                   >  1:2188200/1‑100 (MQ=255)
                                               ccagcGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAAt                                                  <  1:1098282/100‑1 (MQ=255)
                                                cagcGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATa                                                 <  1:2206455/100‑1 (MQ=255)
                                                cagcGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATa                                                 >  2:1573669/1‑100 (MQ=255)
                                                cagcGCCGGTTCCATGCTCATCAGCGTACCAAATGGCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAAt                                                  <  2:1659647/99‑1 (MQ=255)
                                                  gcgcCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGc                                               <  2:107574/100‑1 (MQ=255)
                                                   cgcCGGTTTCATACTCATCAGAGTACCAAATATCCGAGTTGTCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGc                                               <  2:2275699/99‑1 (MQ=255)
                                                    gcCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGa                                             <  1:3333591/100‑1 (MQ=255)
                                                     ccGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGAc                                            <  1:499627/100‑1 (MQ=255)
                                                     ccGGGTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGAc                                            <  2:4121785/100‑1 (MQ=255)
                                                      cGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACa                                           <  1:2544013/100‑1 (MQ=255)
                                                        gTTCCATGCTCATCAGCGTACCAAATGGCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGc                                         <  2:896598/100‑1 (MQ=255)
                                                         ttCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGcc                                        <  2:2600816/100‑1 (MQ=255)
                                                              tGCTCATTAGCGTAACAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGAcc                                   <  2:1531125/100‑1 (MQ=255)
                                                              tGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGAcc                                   >  2:3554384/1‑100 (MQ=255)
                                                                cTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCACAcc                                   >  2:2051230/1‑98 (MQ=255)
                                                                cTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCa                                 >  2:3874536/1‑100 (MQ=255)
                                                                  catcaGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAAt                               >  1:3386351/1‑100 (MQ=255)
                                                                  catcaGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAAt                               >  1:1721748/1‑100 (MQ=255)
                                                                    tcaGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATgg                             <  1:1381571/100‑1 (MQ=255)
                                                                      aGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGaa                           <  1:3422882/100‑1 (MQ=255)
                                                                      aGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGaa                           <  2:2267563/100‑1 (MQ=255)
                                                                      aGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGaa                           <  2:199806/100‑1 (MQ=255)
                                                                        cGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATa                         <  1:1890578/100‑1 (MQ=255)
                                                                        cGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATa                         <  1:3634819/100‑1 (MQ=255)
                                                                         gTACCAAATTTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGTCCCAATGGAATaa                        <  2:2188187/100‑1 (MQ=255)
                                                                            ccAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCTGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCg                     >  2:2841410/1‑100 (MQ=255)
                                                                              aaaTGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGAc                   <  1:3346411/100‑1 (MQ=255)
                                                                              aaaTGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGAc                   <  1:1998135/100‑1 (MQ=255)
                                                                              aaaTGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGAc                   <  1:1425087/100‑1 (MQ=255)
                                                                               aaTGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGAcc                  <  2:1324646/100‑1 (MQ=255)
                                                                               aaTGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGAcc                  <  1:1573681/100‑1 (MQ=255)
                                                                                     cGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGACCAGTGcc            >  2:1604025/1‑100 (MQ=255)
                                                                                       tgtCGGCAAACGCGTGAGGCGAATCCTTTCCAGCGAATAATGCAGAGCGCTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGACCAGTGcc            <  2:3385631/98‑1 (MQ=255)
                                                                                          tGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGCCCCAATGGAATAACCGACCAGTGCCaga         >  2:3831304/1‑98 (MQ=255)
                                                                                          tGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGACCAGTGCCAGAGt       >  1:1568732/1‑100 (MQ=255)
                                                                                           ggCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGACCAGTGCCAGAGTg      <  1:885174/100‑1 (MQ=255)
                                                                                           ggCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGACCAGTGCCAGAGTg      >  1:452691/1‑100 (MQ=255)
                                                                                           ggCAAACGAGTGCGGGCAATCATTTCCAGCGAATAAAGCAGAGCGGACAAGAGAATAGCGACAGACAACCCCAAATGAATAACCGACCAGGCCCagcat       >  2:3994599/1‑96 (MQ=255)
                                                                                           ggCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGACCAGTGCCAGAGTg      >  2:3061802/1‑100 (MQ=255)
                                                                                            gCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGACCAGTGCCAGAGTg      >  2:1011189/1‑99 (MQ=255)
                                                                                              aaaCGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGACCAGTGCCaattggagc   <  1:289575/100‑9 (MQ=255)
                                                                                                   |                                                                                               
TATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGACCAGTGCCAGAGTGCTTC  >  NC_000913/849306‑849500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑