Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 850,998 G→A 16.5% intergenic (+33/+16) ompX → / ← opgE outer membrane protein X/OPG biosynthetic transmembrane phosphoethanolamine transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913850,9980GA16.5% 343.5 / 47.5 150intergenic (+33/+16)ompX/opgEouter membrane protein X/OPG biosynthetic transmembrane phosphoethanolamine transferase
Reads supporting (aligned to +/- strand):  ref base G (62/63);  new base A (9/16);  total (71/79)
Fisher's exact test for biased strand distribution p-value = 2.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 156 total aligned reads displayed.

CGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCC  >  NC_000913/850899‑851097
                                                                                                   |                                                                                                   
  aGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCgggg                                                                                                   >  1:3656490/1‑100 (MQ=255)
        gCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGAtt                                                                                             >  2:3956455/1‑100 (MQ=255)
        gCCGTATTCGTAGCGTGGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCGTCTAATCGCTTTGGTGATATAAAAAATCGGCCTTTCGGGGCgg                                                                                                <  2:3757293/97‑1 (MQ=255)
         ccGTAGTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCGAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAttt                                                                                            <  2:599512/100‑1 (MQ=38)
          cGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAtttt                                                                                           <  1:2961465/100‑1 (MQ=38)
            tATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTg                                                                                         <  2:1239147/100‑1 (MQ=255)
            tATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAtttttt                                                                                         <  2:3417820/100‑2 (MQ=38)
             aTTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAtttttta                                                                                        <  1:3090097/100‑3 (MQ=38)
             aTTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAtttttta                                                                                        <  1:2266655/100‑3 (MQ=38)
              ttCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGtt                                                                                       <  2:1788437/100‑1 (MQ=255)
              ttCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAttttttat                                                                                       <  2:3652017/100‑4 (MQ=37)
               tCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGTGCGGATTTTTGttt                                                                                      <  2:1477072/100‑1 (MQ=255)
               tCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGttt                                                                                      <  2:4099901/100‑1 (MQ=255)
               tCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAttttttata                                                                                      <  1:1548061/100‑5 (MQ=37)
                cGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGtttt                                                                                     <  1:74203/100‑1 (MQ=255)
                cGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGtttt                                                                                     <  1:3974861/100‑1 (MQ=255)
                 gTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGttttt                                                                                    >  2:3111030/1‑100 (MQ=255)
                 gTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGttttt                                                                                    >  1:2597784/1‑100 (MQ=255)
                 gTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAttttttatatc                                                                                    <  1:916260/100‑7 (MQ=37)
                  tAGCTTTGACGTAGGCACCTGGATTTCCGATATTGGTTACCGCGTCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAttttttatat                                                                                     <  2:240465/98‑6 (MQ=21)
                   aGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAttttttatatcac                                                                                  <  1:1318247/100‑9 (MQ=25)
                    gCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAg                                                                                 <  1:3025129/100‑1 (MQ=255)
                    gCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAg                                                                                 <  1:4094432/100‑1 (MQ=255)
                    gCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAg                                                                                 <  2:1870751/100‑1 (MQ=255)
                    gCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAttttttatatcacc                                                                                 <  1:707268/100‑10 (MQ=25)
                    gCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTGGGTGATATAAAAAATACGCCTCTCGGGGCGGATTTTTGTTTTGaa                                                                                  >  2:3065790/1‑99 (MQ=255)
                     cGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAgg                                                                                >  2:3307766/1‑100 (MQ=255)
                     cGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAgg                                                                                <  1:1082493/100‑1 (MQ=255)
                            gTAGGCACCTAGATTGCCGGTGTTGGTTACCGCTTCTAATCCCTTTGGTGATCTAAAAAATCGGGCTCTGGGGGCGGATTTTTGTTTTTTAGGTTTCg                                                                           >  2:1763170/1‑98 (MQ=255)
                             tgtgCACCTTGATTGGCGGTTTTGGTTACCTCTTCTAATTTCTTTGGTTATATAAAAGATTCGCCTCTCTGGGCGGATTGTTGTTTTTAAGGTTTCggg                                                                         <  2:2604173/96‑1 (MQ=255)
                             tAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGt                                                                        >  1:152615/1‑100 (MQ=255)
                             tAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGt                                                                        >  1:3697317/1‑100 (MQ=255)
                               ggCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCg                                                                      <  1:1878215/100‑1 (MQ=255)
                                      ggATTGCCGGTGTTGGATACCGCTTCTAATAACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAatat                                                               >  2:19384/1‑100 (MQ=255)
                                       gATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATc                                                              <  2:2123079/100‑1 (MQ=255)
                                       gATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGAGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATc                                                              <  2:389199/100‑1 (MQ=255)
                                       gATTGCCGGTGTTGGTTACCGCTTCTAATCACTGTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTGTCGGGTCGAAAATATc                                                              >  1:186675/1‑100 (MQ=255)
                                        aTTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCg                                                             <  1:2742328/100‑1 (MQ=255)
                                        aTTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCg                                                             <  2:1952396/100‑1 (MQ=255)
                                         ttGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCgg                                                            <  2:1817908/100‑1 (MQ=255)
                                           gCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGGTTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGtt                                                          >  1:2039848/1‑100 (MQ=255)
                                           gCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGtt                                                          >  1:2294554/1‑100 (MQ=255)
                                           gCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGtt                                                          >  2:3791099/1‑100 (MQ=255)
                                              ggTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCg                                                       >  2:1931882/1‑100 (MQ=255)
                                                tgtTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCgag                                                     <  1:2885958/100‑1 (MQ=255)
                                                  ttGGTTACCGCTTCTAATCACTTTGGTGTTATAAAAAATCCGCCTCTCGGGGCGGATTGTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGAt                                                   <  1:221504/100‑1 (MQ=255)
                                                   tGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCGGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATg                                                  >  2:1791842/1‑100 (MQ=255)
                                                    ggTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATgg                                                 >  1:2472202/1‑100 (MQ=255)
                                                    ggTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATgg                                                 >  1:2319597/1‑100 (MQ=255)
                                                    ggTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATgg                                                 <  2:1612106/100‑1 (MQ=255)
                                                    ggTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATgg                                                 >  1:1724966/1‑100 (MQ=255)
                                                      ttACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGtt                                               >  2:1646969/1‑100 (MQ=255)
                                                       tACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTg                                              <  1:156491/100‑1 (MQ=255)
                                                       tACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTg                                              >  1:864038/1‑100 (MQ=255)
                                                       tACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTg                                              <  1:3956481/100‑1 (MQ=255)
                                                             ttCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATcc                                        <  1:1486129/100‑1 (MQ=255)
                                                             ttCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATcc                                        >  2:1292492/1‑100 (MQ=255)
                                                             ttCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATcc                                        <  1:2978513/100‑1 (MQ=255)
                                                                tAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCAcc                                     >  2:3116084/1‑100 (MQ=255)
                                                                tAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCAcc                                     >  1:3132348/1‑100 (MQ=255)
                                                                 aaTCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCt                                    <  2:2932779/100‑1 (MQ=255)
                                                                  aTCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCtt                                   >  2:331884/1‑100 (MQ=255)
                                                                       tttGGTGATATAAAAAATCCGCTTCTCGGGGTGGATTCTTGTTTTTATGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTa                              <  2:2790969/100‑1 (MQ=255)
                                                                       tttGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTa                              <  1:1074101/100‑1 (MQ=255)
                                                                        ttGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTaa                             >  2:102008/1‑100 (MQ=255)
                                                                        ttGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAAGTTTCGGGTCGAAAATATCGGCTCCGAGATGGTTGTAATCCACCTTCTGTa                              >  2:2105263/1‑99 (MQ=255)
                                                                         tGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAAc                            >  1:516707/1‑100 (MQ=255)
                                                                         tGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAAc                            >  1:2645188/1‑100 (MQ=255)
                                                                          cttaaaaacaaaaaTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACt                           >  1:1172907/10‑100 (MQ=255)
                                                                           gTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTg                          >  1:957483/1‑100 (MQ=255)
                                                                               tataAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAg                      >  1:577989/1‑100 (MQ=255)
                                                                               tataAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAg                      <  2:385263/100‑1 (MQ=255)
                                                                               aaacaaaaaTCCGCCCCGAGAGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCTAGATGGTTGTAATCCACCTTCTGTAACTGGAAg                      >  2:1411810/5‑100 (MQ=37)
                                                                                ataAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATAGGGTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGt                     >  2:553617/1‑100 (MQ=255)
                                                                                ataAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGCCGAAACTATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGGAg                      >  2:234626/1‑99 (MQ=255)
                                                                                 taAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGtt                    >  1:3801289/1‑100 (MQ=255)
                                                                                 acaaaaaTCCGCCCCGAGAGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGtt                    >  1:3399683/3‑100 (MQ=38)
                                                                                  caaaaaTCCGCCCCGAGAGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGCTGGTTGTAATCCACCTTCTGTAACTGGAAGTTg                   >  1:3448923/2‑100 (MQ=38)
                                                                                  caaaaaTCCGCCCCGAGAGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTg                   >  1:2811521/2‑100 (MQ=38)
                                                                                   aaaaaTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTgg                  >  1:2948069/1‑100 (MQ=255)
                                                                                   aaaaaTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTgg                  <  1:46221/100‑1 (MQ=255)
                                                                                   aaaaaTCCGCCCCGAGAGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTgg                  >  1:4097801/1‑100 (MQ=38)
                                                                                   aaaaaTCCGCCCCGAGAGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTgg                  >  1:528822/1‑100 (MQ=38)
                                                                                    aaaaTCCTCCTCTCGGGGTGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGt                 <  2:2834879/100‑1 (MQ=255)
                                                                                    aaaaTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGt                 >  1:3105637/1‑100 (MQ=255)
                                                                                     aaaTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTgg                  <  2:270480/98‑1 (MQ=255)
                                                                                     aaaTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTg                >  1:3426592/1‑100 (MQ=255)
                                                                                      aaTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGa               >  1:1734357/1‑100 (MQ=255)
                                                                                       aTCCGCTACCCGGTGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATCTAGGTTCCGGGATGGTTCGAATCCACCTTCTGTAACTGGAAGTTGGt                 <  2:3627661/97‑1 (MQ=255)
                                                                                       aTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGAt              >  1:43516/1‑100 (MQ=255)
                                                                                        tCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATg             <  1:3568139/100‑1 (MQ=255)
                                                                                          cGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGta           <  2:964088/100‑1 (MQ=255)
                                                                                          cGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGta           <  1:2686632/100‑1 (MQ=255)
                                                                                          cGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGta           >  2:3161180/1‑100 (MQ=255)
                                                                                          cGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGt            <  2:3429031/99‑1 (MQ=255)
                                                                                           gCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGtat          >  2:273507/1‑100 (MQ=255)
                                                                                              tctcGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATc       <  1:3157946/100‑1 (MQ=255)
                                                                                                  ggggCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGAcc   <  1:2248946/100‑1 (MQ=255)
                                                                                                  ggggCGGATGTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGCTTGTAATCCACCTTCTGTAAATGGAAGATTGTCATGtgta         >  2:3895103/1‑94 (MQ=255)
                                                                                                   gggCGGATTTTTGTTTTTAAGGTTTCGGGGCGAAAATATCGGTTGCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGAccc  >  1:3292998/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCC  >  NC_000913/850899‑851097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: