Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 851,094 G→A 25.9% P502S (CCG→TCG)  opgE ← OPG biosynthetic transmembrane phosphoethanolamine transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913851,0940GA25.9% 238.0 / 89.4 147P502S (CCG→TCG) opgEOPG biosynthetic transmembrane phosphoethanolamine transferase
Reads supporting (aligned to +/- strand):  ref base G (53/55);  new base A (19/19);  total (73/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

Only 100 of 152 total aligned reads displayed.

TCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGGAGAAAAAGCCTAAGAAGTCATTGGCTG  >  NC_000913/850995‑851192
                                                                                                   |                                                                                                  
tcGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCgg                                                                                                    <  1:3111049/100‑1 (MQ=255)
 cGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGc                                                                                                   <  1:4083992/100‑1 (MQ=255)
  ggggCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGAcc                                                                                                  <  1:2248946/100‑1 (MQ=255)
     gtgggttgttGTTTTTCAGCTTTCGGTTCGCAAATCGCGGTGCCCAGATGGTTGTAATCCACTTTCTGTACCTCGAAGTTGCTTATGTGTATCGGCCCg                                                                                                <  2:1220329/91‑1 (MQ=255)
       ngATTTTTGTTTTGAAGGTTTCGGGTCGAAAGTGTCGGTTCCTAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGACCCGGCt                                                                                             >  1:2792208/2‑100 (MQ=255)
       ggATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGACCCGGCt                                                                                             >  2:2844586/1‑100 (MQ=255)
          tttttGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTttc                                                                                          <  2:3056585/100‑1 (MQ=255)
           ttttGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGACCCGGCTttct                                                                                         <  1:3018127/100‑1 (MQ=255)
             ttGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTttcttc                                                                                       <  1:397620/100‑1 (MQ=255)
              tGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTtcttct                                                                                      >  1:3962321/1‑100 (MQ=255)
               gTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTtctc                                                                                     <  2:3266003/100‑1 (MQ=255)
               gTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTtctc                                                                                     >  1:439702/1‑100 (MQ=255)
                tttttAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCa                                                                                    >  1:741055/1‑100 (MQ=255)
                 ttttAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCTGCTTTCTTCTCAg                                                                                   <  1:1473918/100‑1 (MQ=255)
                 ttttAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGACCCGGCTTTCTTCTCAg                                                                                   <  1:1208515/100‑1 (MQ=255)
                      aGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGAtata                                                                              <  1:881720/100‑1 (MQ=255)
                      aGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGACCCGGCTTTCTTCTCAGAtata                                                                              <  1:1078148/100‑1 (MQ=255)
                       ggTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATaa                                                                             >  2:1030815/1‑100 (MQ=255)
                       ggTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGACCCGGCTTTCTTCTCAGatat                                                                               >  2:3588563/1‑98 (MQ=255)
                       ggTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGACCCGGCTTTCTTCTCAGAtcta                                                                              >  2:381880/1‑99 (MQ=255)
                         tttCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAAc                                                                           >  1:248369/1‑100 (MQ=255)
                          ttCGGGTCGAAAATATCGGTTCCGATATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACg                                                                          <  2:1070531/100‑1 (MQ=255)
                            cGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGACCCGGCTTTCTTCTCAGATATAAACggg                                                                        <  1:3390944/100‑1 (MQ=255)
                             gggTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACgg                                                                         >  2:2213245/1‑98 (MQ=255)
                             gggTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGt                                                                       <  2:3881918/100‑1 (MQ=255)
                               gTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTa                                                                      <  2:4018004/99‑1 (MQ=255)
                                 cGAAAATATCGGTTGCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTAttt                                                                   <  2:957522/100‑1 (MQ=255)
                                 cGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTAttt                                                                   <  1:2884993/100‑1 (MQ=255)
                                   aaaaTATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTa                                                                      >  2:1212785/1‑95 (MQ=255)
                                    aaaTATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGAt                                                                <  1:518605/100‑1 (MQ=255)
                                     aaTATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATg                                                               <  1:2761338/100‑1 (MQ=255)
                                     aaTATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATg                                                               <  2:516702/100‑1 (MQ=255)
                                     aaTATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGACCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATg                                                               <  1:2591903/100‑1 (MQ=255)
                                      atatCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGt                                                              >  2:20844/1‑100 (MQ=255)
                                       tatCGGTTTGGAGATGGTTGTAATCCATCTTCTGTAACTGTAAGTGGGTGATGTATATCTGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTCAt                                                                <  2:2454976/97‑1 (MQ=255)
                                       tatCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGtt                                                             <  1:3025373/100‑1 (MQ=255)
                                       tatCGGTTCCGAGATGGTTGTAATACACGTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGTTATTTTCTGtt                                                             >  1:2760911/1‑100 (MQ=255)
                                        atCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGACCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTa                                                            <  2:1764/100‑1 (MQ=255)
                                          cGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAAt                                                          >  2:3793598/1‑100 (MQ=255)
                                           ggTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAAtt                                                         >  2:2419404/1‑100 (MQ=255)
                                           ggTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGACCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAAtt                                                         >  1:3855740/1‑100 (MQ=255)
                                            gTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAAttt                                                        <  1:347555/100‑1 (MQ=255)
                                            gTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGACCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAAttt                                                        >  1:2931372/1‑100 (MQ=255)
                                             ttCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTc                                                       >  2:4099376/1‑100 (MQ=255)
                                              tCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTcc                                                      <  1:1070806/100‑1 (MQ=255)
                                               ccGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCt                                                     >  1:4030457/1‑100 (MQ=255)
                                                 gagaTGGTTTTAATCCACCTTCTGTACTTGTAAGTTGGTGATGTATATCGGCCCGGCTTTCTTATCAGATATAAACGGGTATTTCATGTTAATTTCCTTc                                                   <  2:2549157/100‑1 (MQ=255)
                                                 gagaTGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTc                                                   >  1:1952505/1‑100 (MQ=255)
                                                   gaTGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGc                                                 >  2:1935621/1‑100 (MQ=255)
                                                    aTGGTTGTATTCCACCTTCTGTAATTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGc                                                 <  2:2060805/99‑1 (MQ=255)
                                                     tGGTTGTAATGCACCTTCTGTAACTGGAAGTTGGTGTTGTATATCGACCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCt                                                <  2:4067950/99‑1 (MQ=255)
                                                      ggTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCttt                                              <  1:3503838/100‑1 (MQ=255)
                                                      ggTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGACCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCttt                                              <  1:2194105/100‑1 (MQ=255)
                                                       gTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGCAATAAACGGGGATTTGATGTTAATTTCCTTCGCttt                                              >  2:1546296/1‑99 (MQ=255)
                                                       gTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCctt                                              >  2:3274977/1‑99 (MQ=255)
                                                       gTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTa                                             >  1:464869/1‑100 (MQ=255)
                                                        ttGTAATCCACCTTCTGTAACTGGACGTTGGTGCTGTATATCGTCCTTGCTTTCTCTTCAGGTATCAAAGGGAGTTTGCTGTTAATTTTCtt                                                    >  2:3418877/1‑92 (MQ=255)
                                                           tAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTGGATGTTAATTTCCTTCGCTTTaa                                            >  2:3409815/1‑97 (MQ=255)
                                                           tAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGACCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATcc                                         >  1:3302295/1‑100 (MQ=255)
                                                            aaTCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGACCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATccc                                        >  2:748558/1‑100 (MQ=255)
                                                             aTCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATcccc                                       >  1:2657814/1‑100 (MQ=255)
                                                              tCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCg                                      <  2:224837/100‑1 (MQ=255)
                                                                cACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTc                                    >  2:3889532/1‑100 (MQ=255)
                                                                cACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTc                                    >  1:2480724/1‑100 (MQ=255)
                                                                 aCCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTcc                                   <  1:3262871/100‑1 (MQ=255)
                                                                    ttCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATGAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACt                                <  2:2294541/100‑1 (MQ=255)
                                                                    ttCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACt                                <  1:2088610/100‑1 (MQ=255)
                                                                     tCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTg                               <  2:864032/100‑1 (MQ=255)
                                                                     tCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTg                               <  1:733989/100‑1 (MQ=255)
                                                                      cTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATACACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTgg                              >  1:168374/1‑100 (MQ=255)
                                                                       tGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTggg                             >  1:3966129/1‑100 (MQ=255)
                                                                        gTAACTGGAATTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTGAATTTCCTTCGCTTTAATCCCCGTCCACTGGga                            <  2:2720009/100‑1 (MQ=255)
                                                                        gTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGga                            >  2:3037669/1‑100 (MQ=255)
                                                                         tAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGgag                           <  1:331887/100‑1 (MQ=255)
                                                                         tAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGgag                           <  1:579712/100‑1 (MQ=255)
                                                                         tAACTGGAAGTTGGTGATGTATATCGACCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGgag                           <  1:2611108/100‑1 (MQ=255)
                                                                          aaCTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGgaga                          >  2:2139979/1‑100 (MQ=255)
                                                                          aaCTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGgaga                          >  1:784102/1‑100 (MQ=255)
                                                                           aCTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGGAGaa                         >  2:429070/1‑100 (MQ=255)
                                                                           aCTGGAAGTTGGTGATGTATATCGACCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGGAGaa                         <  2:1568875/100‑1 (MQ=255)
                                                                           aCTGGAAGTTGGTGATGTATATCGACCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGGAGaa                         >  1:2645354/1‑100 (MQ=255)
                                                                            cTGGAAGTTGGTGATGTATATCGACCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCACCGTCCAGTGGGAGaa                         >  2:3628307/1‑99 (MQ=255)
                                                                              ggAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGGAGaaaaa                      >  2:1417122/1‑100 (MQ=255)
                                                                              ggAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGGAGaaaaa                      >  1:3251638/1‑100 (MQ=255)
                                                                                aaGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGGAGAAAAAGc                    <  1:3869228/100‑1 (MQ=255)
                                                                                 aGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGGAGAAAAAGcc                   >  1:1687239/1‑100 (MQ=255)
                                                                                   ttGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGGAGAAAAAGCCTa                 >  1:765342/1‑100 (MQ=255)
                                                                                     ggTGATGTATATCGACCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGGAGAAAAAGCCTaag               >  1:2276060/1‑100 (MQ=255)
                                                                                      gTGATGTATATCGACCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGGAGAAAAAGCCTaaga              <  1:3116046/100‑1 (MQ=255)
                                                                                        gATGTATATCGACCCGGCTTTCTTCTCAGATATAAACGGGAATTTGATGTTAATTAACTTCGCTTTAATCCCCGTCCACTGGGAGAAAAAGCCTaagaag            >  1:3712827/1‑100 (MQ=255)
                                                                                         aTGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTTATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGGAGAAAAAGCCTAAGAAGt           <  2:1781273/100‑1 (MQ=255)
                                                                                            tataTCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGGAGAAAAAGCCTAAGAAGTCAt        >  1:2379035/1‑100 (MQ=255)
                                                                                             atatCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGGAGAAAAAGCCTAAGAAGTCAtt       >  1:1362706/1‑100 (MQ=255)
                                                                                              tatCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGGAGAAAAAGCCTAAGAAGTCATTg      <  2:4030431/100‑1 (MQ=255)
                                                                                               atCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGGAGAAAAAGCCTAAGAAGTCATTgg     <  2:1409344/100‑1 (MQ=255)
                                                                                               atCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATACACGTCCACTGGGAGAAAAAGTCTCAGACGTCATTgg     >  2:2925913/1‑100 (MQ=255)
                                                                                                tCGACCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGGAGAAAAAGCCTAAGAAGTCATTGGc    >  1:1028068/1‑100 (MQ=255)
                                                                                                tCGACCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGGAGAAAAAGCCTAAGAAGTCATTGGc    >  1:1029438/1‑100 (MQ=255)
                                                                                                   gCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGGAGAAAAAGCCTAAGAAGTCATTGGCTg  >  1:954837/1‑99 (MQ=255)
                                                                                                   gCCCGGCTTTCTTCTCAGATATAAACGGGGATTTGATGTTAATTTCCTTCGCTTGAATCCCCGTCCACTGGGAGAAAAAGCCTAAGAAGTCATTGGCt   <  1:1455455/98‑1 (MQ=255)
                                                                                                   |                                                                                                  
TCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTTCGCTTTAATCCCCGTCCACTGGGAGAAAAAGCCTAAGAAGTCATTGGCTG  >  NC_000913/850995‑851192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: