Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 905,720 C→A 18.1% L270M (CTG→ATG)  amiD → 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase, Zn‑dependent; OM lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913905,7200CA18.1% 366.7 / 62.6 146L270M (CTG→ATG) amiD1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase, Zn‑dependent; OM lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (49/69);  new base A (18/9);  total (67/79)
Fisher's exact test for biased strand distribution p-value = 2.02e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 148 total aligned reads displayed.

AGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGAT  >  NC_000913/905622‑905819
                                                                                                  |                                                                                                   
 gcagcGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCCTTGATg                                                                                                   >  2:226761/1‑100 (MQ=255)
 gcagcGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCt                                                                                                    <  1:3538457/99‑1 (MQ=255)
  cagcTGCGCGTGATTATGGCATTCCAGATGCATTTCCGTCCGACGTTATATAACGGCGAAGCGAATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTgg                                                                                                  <  1:1234082/100‑1 (MQ=255)
  cagcGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTgg                                                                                                  >  2:1141743/1‑100 (MQ=255)
  cagcGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTgg                                                                                                  >  2:468307/1‑100 (MQ=255)
    gcggggCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGga                                                                                                 <  2:1579323/99‑1 (MQ=255)
    gcggcgCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGgag                                                                                                >  1:2549899/1‑100 (MQ=255)
     cggcgCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGgaga                                                                                               <  2:2801360/100‑1 (MQ=255)
       gcgcGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTACCGAAGCATTGCTGGAGaaa                                                                                             <  2:3092601/100‑1 (MQ=255)
       gcgcGTGATTAAGGCATTCCAGATGCATTTCCGCCCGACGTTAGATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGAATTGATGgag                                                                                                <  2:4074110/97‑1 (MQ=255)
          cgggATTATGGCATTCCAGATGCATTGTCGCCCGACGTTATATAGCGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATa                                                                                           <  2:4966/96‑1 (MQ=255)
            tGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACgg                                                                                        <  1:3422743/100‑1 (MQ=255)
             ggttATGGCATTCCAGATGCAGGTCCGCCCGACGTTATATGACGGCGAAGCGGAGGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACggg                                                                                       <  2:4061641/98‑1 (MQ=255)
             gATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACggg                                                                                       >  2:2648031/1‑100 (MQ=255)
             gATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACggg                                                                                       <  1:2047180/100‑1 (MQ=255)
             gATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACggg                                                                                       >  1:2273418/1‑100 (MQ=255)
              aTTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGc                                                                                      <  2:570897/100‑1 (MQ=255)
              aTTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGc                                                                                      >  2:2534998/1‑100 (MQ=255)
              aTTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGc                                                                                      <  1:2404856/100‑1 (MQ=255)
                 aTGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAgg                                                                                   >  2:2827109/1‑100 (MQ=255)
                   ggCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGa                                                                                  <  1:3785091/99‑1 (MQ=255)
                      aTTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAg                                                                              >  1:2235959/1‑100 (MQ=255)
                         ccAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAgcgc                                                                           <  2:3831465/100‑1 (MQ=255)
                           aGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCgg                                                                         <  2:1192795/100‑1 (MQ=255)
                           aGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCgg                                                                         >  1:2187668/1‑100 (MQ=255)
                           aGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCgg                                                                         <  1:1246563/100‑1 (MQ=255)
                            gATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGc                                                                        <  2:1468250/100‑1 (MQ=255)
                              ttcATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAg                                                                      <  1:3212553/98‑1 (MQ=255)
                              tGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGccg                                                                      >  1:380885/1‑98 (MQ=255)
                               gCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGt                                                                     >  1:2851899/1‑100 (MQ=255)
                                cATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGAGCAGGATCAGCGCGGCAGtt                                                                    >  2:1694047/1‑100 (MQ=255)
                                cATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGc                                                                        >  1:859921/1‑96 (MQ=255)
                                  tttCCGCCCGACGTTATATAACGGCGAAGCGGTTGCAGCAACTCAGGCGATTGCCGAAGCATTGATGGATAAATACGGGCCGGATTAGCGCGGCAGttt                                                                   >  2:2099563/1‑99 (MQ=255)
                                   ttCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTc                                                                 <  1:3252947/100‑1 (MQ=255)
                                    tCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTcc                                                                <  2:4047003/100‑1 (MQ=255)
                                      cGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCGGt                                                              >  2:602806/1‑100 (MQ=255)
                                      cGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGt                                                              >  2:2064420/1‑100 (MQ=255)
                                       gCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTg                                                             <  2:108907/100‑1 (MQ=255)
                                       gCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTg                                                             >  1:100175/1‑100 (MQ=255)
                                       gCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTg                                                             >  2:1005026/1‑100 (MQ=255)
                                        cccGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTgg                                                            >  2:2623467/1‑100 (MQ=255)
                                         ccGACGCCATACAACGTCGAAGTTGATGCAGAAACTCATGCGATTGCTTAAGCATTGCTGGAGAAATACGTGCACGATTAGCGCGCCAGTTTCCCGTg                                                             <  2:1483271/98‑1 (MQ=255)
                                          cGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTc                                                          >  2:870663/1‑100 (MQ=255)
                                          cGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTc                                                          >  2:802011/1‑100 (MQ=255)
                                             cGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTcgcg                                                       <  1:1127234/100‑1 (MQ=255)
                                               ttATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTa                                                     <  1:154316/100‑1 (MQ=255)
                                                 atataACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGc                                                   <  1:1855953/100‑1 (MQ=255)
                                                    taACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAg                                                >  1:1678761/1‑100 (MQ=255)
                                                      aCGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAggc                                              >  2:2280702/1‑100 (MQ=255)
                                                      aCGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAggc                                              <  2:335195/100‑1 (MQ=255)
                                                        ggCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAggcgg                                            >  1:2691356/1‑100 (MQ=255)
                                                         gCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAggcggc                                           <  1:2648047/100‑1 (MQ=255)
                                                           gAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAg                                         <  1:1933231/100‑1 (MQ=255)
                                                            aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAg                                         <  2:2150914/99‑1 (MQ=255)
                                                            aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGt                                        >  2:1344259/1‑100 (MQ=255)
                                                            aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAg                                         >  2:1312619/1‑99 (MQ=255)
                                                              gCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGttt                                      >  2:39444/1‑100 (MQ=255)
                                                              gCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGttt                                      <  1:985130/100‑1 (MQ=255)
                                                              gCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGttt                                      <  1:1923058/100‑1 (MQ=255)
                                                                ggATGGAGAGACGCAGGCGAGTGCCGAAGGATTGGTGGAGAGAGACGGGGGGGGTGAGCGCGGCGGTTGTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtc                                    <  2:1120319/100‑1 (MQ=255)
                                                                  aTGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtctc                                  <  2:2968263/100‑1 (MQ=255)
                                                                  aTGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtctc                                  <  1:3918041/100‑1 (MQ=255)
                                                                  aTGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtctc                                  <  1:1869849/100‑1 (MQ=255)
                                                                  aTGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtctc                                  >  1:2411791/1‑100 (MQ=255)
                                                                   tGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCg                                 <  2:3283754/100‑1 (MQ=255)
                                                                   tGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCg                                 >  1:3116712/1‑100 (MQ=255)
                                                                    gCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa                                >  2:3664417/1‑100 (MQ=255)
                                                                     cAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGTGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGAt                               <  2:2907164/100‑1 (MQ=255)
                                                                     cAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGAt                               >  2:3126256/1‑100 (MQ=255)
                                                                     cAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGAt                               >  1:3418023/1‑100 (MQ=255)
                                                                      aGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATa                              <  1:560483/100‑1 (MQ=255)
                                                                      aGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATa                              >  2:697710/1‑100 (MQ=255)
                                                                      aGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATa                              >  2:704327/1‑100 (MQ=255)
                                                                        aaaCTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATAcc                            <  1:1626265/100‑1 (MQ=255)
                                                                         aaCTCAGGCGTTTGCCGAAGCATTGCTGGAGAAATACAAGCAGGATTAGCGCGGCAGATTTCCGTGGTCACGTAGGCAGGCGGCAGTTTTCTCGATACCt                           >  2:3675010/1‑100 (MQ=255)
                                                                           cTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTc                         >  1:1051015/1‑100 (MQ=255)
                                                                            tCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCa                        <  1:4135088/100‑1 (MQ=255)
                                                                               ggCGATTGGCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATcc                     <  2:1030490/100‑1 (MQ=255)
                                                                               ggCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATcc                     >  1:3174819/1‑100 (MQ=255)
                                                                                gCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTAGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATc                      >  2:3523812/1‑98 (MQ=255)
                                                                                  gATTGCCGTGGTATTGCGGGAGATATCCTGGCAGGAGTCGCGCGGCTTTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGAt                               <  2:2378970/87‑1 (MQ=255)
                                                                                  gATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAgg                  <  1:2678341/100‑1 (MQ=255)
                                                                                  gATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAgg                  <  1:477085/100‑1 (MQ=255)
                                                                                     tGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtg               >  1:1676336/1‑100 (MQ=255)
                                                                                     tGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtg               <  1:4120829/100‑1 (MQ=255)
                                                                                      gCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtga              <  1:2827125/100‑1 (MQ=255)
                                                                                      gCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtga              >  1:731891/1‑100 (MQ=255)
                                                                                      gCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtga              <  1:3978807/100‑1 (MQ=255)
                                                                                       ccGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGCTACCTTCATCCAGGGtgat             >  2:3364809/1‑100 (MQ=255)
                                                                                       ccGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtgat             >  1:2490126/1‑100 (MQ=255)
                                                                                        cGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtgatg            <  2:3522855/100‑1 (MQ=255)
                                                                                        cGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtgatg            <  1:580401/100‑1 (MQ=255)
                                                                                        cGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAACCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtgatg            <  1:870669/100‑1 (MQ=255)
                                                                                         gAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtgatga           <  1:1265319/100‑1 (MQ=255)
                                                                                         gAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtgatga           >  2:4122131/1‑100 (MQ=255)
                                                                                             cATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCgg       <  2:1970543/100‑1 (MQ=255)
                                                                                             cATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCgg       >  1:2279734/1‑100 (MQ=255)
                                                                                               ttGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCt     <  1:1421670/100‑1 (MQ=255)
                                                                                                  cTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGAt  <  1:2078057/100‑1 (MQ=255)
                                                                                                  cTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGAt  >  1:3525201/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
AGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGAT  >  NC_000913/905622‑905819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑