Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 914,948 A→G 61.4% intergenic (‑91/+404) ybjE ← / ← aqpZ putative transporter/aquaporin Z

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913914,9480AG61.4% 120.2 / 138.6 138intergenic (‑91/+404)ybjE/aqpZputative transporter/aquaporin Z
Reads supporting (aligned to +/- strand):  ref base A (23/30);  new base G (49/35);  total (72/66)
Fisher's exact test for biased strand distribution p-value = 1.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

GAAAACATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCC  >  NC_000913/914850‑915046
                                                                                                  |                                                                                                  
gAAAACATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAAt                                                                                                   >  1:2962987/1‑100 (MQ=255)
 aaaaCATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATg                                                                                                  >  1:2521116/1‑100 (MQ=255)
  aaaCATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAgtgt                                                                                                 >  2:2870272/1‑100 (MQ=255)
  aaaCATGAAAACCCACTCCTTGCAAAGATGAAGTCTAAATGCGCTATGACACACTACTCAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATg                                                                                                  <  2:3522127/99‑1 (MQ=255)
    aCATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTa                                                                                               <  2:782826/100‑1 (MQ=255)
     cATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTAt                                                                                              <  1:2135075/100‑1 (MQ=255)
     cATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACAATACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTAt                                                                                              <  2:2811987/100‑1 (MQ=255)
      aTGTAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATg                                                                                             <  2:1620586/100‑1 (MQ=255)
      aTGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATg                                                                                             <  2:3398969/100‑1 (MQ=255)
       tGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGc                                                                                            >  1:3024370/1‑100 (MQ=255)
       tGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGc                                                                                            >  2:238089/1‑100 (MQ=255)
        gAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCa                                                                                           <  2:1044668/100‑1 (MQ=255)
        gAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCa                                                                                           >  2:2249677/1‑100 (MQ=255)
         aaaaCCCAGTCCTCGCAAAGATGAAGTCGAAGTGCGCGATGAGACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCAt                                                                                          >  1:2789241/1‑100 (MQ=255)
           aaCCAAGTCCTTTCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAGTTAAAAGGAAATGTTATGCATa                                                                                         <  2:1201447/99‑1 (MQ=255)
             cccAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAgg                                                                                      >  1:797357/1‑100 (MQ=255)
              ccAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGa                                                                                     >  2:1925182/1‑100 (MQ=255)
              ccAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGa                                                                                     <  2:2219405/100‑1 (MQ=255)
                aGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGc                                                                                   <  1:4008880/100‑1 (MQ=255)
                aGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGc                                                                                   >  1:2612199/1‑100 (MQ=255)
                   ccTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGt                                                                                <  1:2006630/100‑1 (MQ=255)
                   ccTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGt                                                                                >  1:2202574/1‑100 (MQ=255)
                   ccTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGt                                                                                >  1:979064/1‑100 (MQ=255)
                   ccTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGAGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGt                                                                                >  2:2292511/1‑100 (MQ=255)
                    cTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTa                                                                               <  1:1256166/100‑1 (MQ=255)
                    cTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTa                                                                               <  2:1180275/100‑1 (MQ=255)
                    cTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTa                                                                               <  1:2856103/100‑1 (MQ=255)
                     ttGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTag                                                                              <  2:860879/100‑1 (MQ=255)
                      tGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTaga                                                                             >  2:1679708/1‑100 (MQ=255)
                        cAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGt                                                                           >  2:1432185/1‑100 (MQ=255)
                        cAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGt                                                                           >  1:356709/1‑100 (MQ=255)
                          aaGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTAt                                                                         <  1:2231420/100‑1 (MQ=255)
                          aaGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTAt                                                                         >  1:167743/1‑100 (MQ=255)
                            gATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTc                                                                       >  2:4078719/1‑100 (MQ=255)
                            gATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTc                                                                       >  1:1081779/1‑100 (MQ=255)
                             aTGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCg                                                                      <  1:261026/100‑1 (MQ=255)
                              tGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGt                                                                     >  2:3925912/1‑100 (MQ=255)
                              tGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGt                                                                     >  2:1638275/1‑100 (MQ=255)
                                  gTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTc                                                                 <  1:1809675/100‑1 (MQ=255)
                                   tCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCa                                                                <  1:193931/100‑1 (MQ=255)
                                    cGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCAt                                                               <  1:3664674/100‑1 (MQ=255)
                                     gAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCAtt                                                              <  2:1230271/100‑1 (MQ=255)
                                       aaTGCGCGATGACAAACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTa                                                            <  2:3532865/100‑1 (MQ=255)
                                        aTGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTaa                                                           <  2:4036808/100‑1 (MQ=255)
                                        aTGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTaa                                                           >  1:1571889/1‑100 (MQ=255)
                                         tGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTaaa                                                          <  2:1093679/100‑1 (MQ=255)
                                          gcgcGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAg                                                         <  1:3724029/100‑1 (MQ=255)
                                          gcgcGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAg                                                         >  1:536696/1‑100 (MQ=255)
                                           cgcgATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGa                                                        >  1:1918057/1‑100 (MQ=255)
                                            gcgATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGat                                                       <  1:28386/100‑1 (MQ=255)
                                             ccaTGACACACTACCGAACGCGGAACAACGAGTAAAGTCGCAATTAAAAGGAACTGTTATGCATAAGAAGCAGAAAAGTATTCGTTTTCATTTAAAg                                                         <  2:2826317/95‑1 (MQ=255)
                                                 gACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCt                                                  <  1:3544654/100‑1 (MQ=255)
                                                   cacacTACTGAAAACGGAAGGACAAGTAAAGTTGAAATTAAAAGGAAATTTTGTGCATAAGTAGCAGTACAGCATTCGTTTTCATTTAAAGATATTCTTg                                                <  2:1007989/100‑1 (MQ=255)
                                                    acacTACTGAAAGCGGAAGGACGAGTGAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTgc                                               >  1:2508071/1‑100 (MQ=255)
                                                    acacTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTgc                                               >  1:1626341/1‑100 (MQ=255)
                                                    acacTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTgc                                               <  1:2761776/100‑1 (MQ=255)
                                                    acacTACTGAAAGAGGGAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTgc                                               <  2:1081209/100‑1 (MQ=255)
                                                     cacTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTgcg                                              >  1:2287529/1‑100 (MQ=255)
                                                     cacTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTgcg                                              >  1:3993482/1‑100 (MQ=255)
                                                     cacTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTgc                                               <  2:1081222/99‑1 (MQ=255)
                                                       ctactGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCt                                            >  2:3870394/1‑100 (MQ=255)
                                                        tactGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGAATTCGTTTTCATTTAAAGATATTCTTGCGCtt                                           >  2:4019578/1‑100 (MQ=255)
                                                         actGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCttt                                          >  2:1938118/1‑100 (MQ=255)
                                                         actGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCttt                                          >  1:1412100/1‑100 (MQ=255)
                                                           tGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTaa                                        >  1:700064/1‑100 (MQ=255)
                                                           tGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTaa                                        <  1:3613693/100‑1 (MQ=255)
                                                            gAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATCTTCTTGCGCTTTAAt                                       >  1:4068903/1‑100 (MQ=255)
                                                            gAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAAt                                       >  2:322980/1‑100 (MQ=255)
                                                            gAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAAt                                       >  1:742816/1‑100 (MQ=255)
                                                            gAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAAt                                       >  1:899735/1‑100 (MQ=255)
                                                                 cGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACaa                                  >  2:2674260/1‑100 (MQ=255)
                                                                   naaGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAAc                                >  2:2020790/2‑100 (MQ=255)
                                                                   gAAGGACGAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAAc                                <  1:2183251/100‑1 (MQ=255)
                                                                   gAAGGACGAGTAAAGTTGCAATTAAAAGGAAATTTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAAc                                <  2:586309/100‑1 (MQ=255)
                                                                   gAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAAc                                <  1:579878/100‑1 (MQ=255)
                                                                         cGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCAcc                          <  2:1412091/100‑1 (MQ=255)
                                                                          gAGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCg                         >  2:2037849/1‑100 (MQ=255)
                                                                          gAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCg                         >  2:1365214/1‑100 (MQ=255)
                                                                           aGTAAAGTTGCAATTAAAAGGACGTGTTATGCATAAGTAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGa                        <  2:2785923/100‑1 (MQ=255)
                                                                           aGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGa                        <  1:1482603/100‑1 (MQ=255)
                                                                           aGTAAAGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGa                        <  1:503570/100‑1 (MQ=255)
                                                                           aGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGa                        >  2:3974877/1‑100 (MQ=255)
                                                                            gTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGAt                       >  1:3067082/1‑100 (MQ=255)
                                                                               aaGTTGCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGtt                    >  1:166942/1‑100 (MQ=255)
                                                                                    gCAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTtggtgg               <  2:547373/100‑1 (MQ=255)
                                                                                     cAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTtggtgcc              >  2:818192/1‑98 (MQ=255)
                                                                                     cAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGc              >  1:1588571/1‑100 (MQ=255)
                                                                                     cAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGc              >  2:3269886/1‑100 (MQ=255)
                                                                                     cAATTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGc              >  1:4001849/1‑100 (MQ=255)
                                                                                       aTTAAAAGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGt            >  2:189320/1‑100 (MQ=255)
                                                                                       aTTAAAAGGAAGTGGTATGCATAAGGAGCAGTAGAGTAGTCGTTTTCATTTAAAGATATTCTTCCGTTTTAATTACAAGCTGCACCGATGTtggtgg               <  2:2716211/97‑1 (MQ=255)
                                                                                        ttAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTc           <  1:2628300/100‑1 (MQ=255)
                                                                                          aaaaGGAAGTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCaa         >  2:1297632/1‑100 (MQ=255)
                                                                                          aaaaGGAAGTGTTATGCATAAGGAGCAGTAGAGTAATCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCaa         >  2:1871188/1‑100 (MQ=255)
                                                                                           aaaGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTcc          <  2:919201/98‑2 (MQ=255)
                                                                                            aaGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCaaaa       <  2:194647/100‑1 (MQ=255)
                                                                                              ggAAGTGATATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAACTACAAACTGCACCGATGTTGGTGGCGTCAAAATc     >  2:2167442/1‑100 (MQ=255)
                                                                                              ggAAATGTTATGCATAAGGAGCAGTAGAGGATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATc     <  1:1025467/100‑1 (MQ=255)
                                                                                               gAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCg    <  1:2302399/100‑1 (MQ=255)
                                                                                                 aaTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGcc  <  2:2553543/100‑1 (MQ=255)
                                                                                                  |                                                                                                  
GAAAACATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCC  >  NC_000913/914850‑915046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: