Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 931,952 C→T 19.0% V33V (GTG→GTA trxB ← thioredoxin reductase, FAD/NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913931,9520CT19.0% 268.6 / 48.2 121V33V (GTG→GTAtrxBthioredoxin reductase, FAD/NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (53/44);  new base T (13/10);  total (66/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 129 total aligned reads displayed.

CATTAATAACGGACCGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGTTTGGTCGTGCCCATG  >  NC_000913/931853‑932051
                                                                                                   |                                                                                                   
cATTAATAACGGACCGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGc                                                                                                     <  1:918017/100‑1 (MQ=255)
 aTTAATAACGGACCGGTCAGATCGGTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGca                                                                                                    >  2:3585662/1‑100 (MQ=255)
  ttAATAACGGACCGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGtac                                                                                                   <  2:2566949/100‑1 (MQ=255)
   taataaCGGACCGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGcaca                                                                                                  <  1:1099408/100‑1 (MQ=255)
   taataaCGGACCGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGcac                                                                                                   >  2:3610709/1‑99 (MQ=255)
     ataaCGGACCGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCAcagg                                                                                                >  1:784663/1‑100 (MQ=255)
     ataaCGGACCGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCAcagg                                                                                                >  1:2741518/1‑100 (MQ=255)
      taaCGGACCGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTAcaggt                                                                                               >  2:1507197/1‑100 (MQ=255)
      taaCGGACCGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTAcaggt                                                                                               <  1:3740739/100‑1 (MQ=255)
      taaCGGACCGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCAcaggt                                                                                               >  1:1557385/1‑100 (MQ=255)
      taaCGGACCGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCAcaggt                                                                                               >  1:3804420/1‑100 (MQ=255)
        aCGGACCGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCAcaggttg                                                                                             <  2:723397/100‑1 (MQ=255)
            aCCGGTCAGACCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTCTCCATGCCGGTAATCAGTAcaggttgcagg                                                                                         <  2:2719900/100‑1 (MQ=255)
              cGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTAcaggttgcaggtt                                                                                       <  1:457125/100‑1 (MQ=255)
              cGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCAcaggttgcaggt                                                                                        <  2:3882262/99‑1 (MQ=255)
                gTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTgg                                                                                     <  2:1040528/100‑1 (MQ=255)
                gTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTgg                                                                                     <  2:2867431/100‑1 (MQ=255)
                gTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTgg                                                                                     >  2:1564962/1‑100 (MQ=255)
                gTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTACATGCCGGTAATCAGTAcaggttgcaggt                                                                                        >  2:2089429/1‑97 (MQ=255)
                gTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGAGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGACGGTAATCAGCAcaggttgcaggttag                                                                                     >  2:2868583/1‑98 (MQ=255)
                  cAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGgcg                                                                                   <  1:2635727/100‑1 (MQ=255)
                  cAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGgcg                                                                                   >  2:3800122/1‑100 (MQ=255)
                  cAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGgcg                                                                                   <  1:3432253/100‑1 (MQ=255)
                    gATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGgcgcg                                                                                 >  1:1407897/1‑100 (MQ=255)
                        gTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGcgcc                                                                             >  2:1301176/1‑100 (MQ=255)
                           tGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCcgcg                                                                          <  1:1965265/100‑1 (MQ=255)
                           tGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCcgcg                                                                          <  1:1964811/100‑1 (MQ=255)
                           nggATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCcgcg                                                                          >  1:154704/2‑100 (MQ=255)
                             gATTGCCAGGCCCGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTGCATGTTGCAGTTTGGCGCGCGCCGCGTa                                                                        >  2:3958973/1‑100 (MQ=255)
                             gATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTATTCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTa                                                                        >  2:2932250/1‑100 (MQ=255)
                              aTCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAg                                                                       >  1:1494830/1‑100 (MQ=255)
                               tCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGa                                                                      <  1:2687301/100‑1 (MQ=255)
                                cGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGAc                                                                     <  1:2612175/100‑1 (MQ=255)
                                cGCCAGGCAAGTTTTCCACTTCCGGGGTGGTGGTCAGTTTGCCGCGTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGAc                                                                     >  2:342131/1‑100 (MQ=255)
                                 gccaggccagTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGAca                                                                    >  1:1808759/1‑100 (MQ=255)
                                  ccaggccagTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGAcag                                                                   <  1:3745550/100‑1 (MQ=255)
                                  ccaggccagTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGAcag                                                                   <  1:778302/100‑1 (MQ=255)
                                   caggccagTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGAcagc                                                                  <  1:619815/100‑1 (MQ=255)
                                   caggccagTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGAcagc                                                                  >  1:1738793/1‑100 (MQ=255)
                                     ggccagTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGAcagcag                                                                >  1:4092570/1‑100 (MQ=255)
                                          gTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGgtg                                                           <  1:1667012/100‑1 (MQ=255)
                                          gTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGgtg                                                           <  1:1606951/100‑1 (MQ=255)
                                          gTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGgtg                                                           <  2:73626/100‑1 (MQ=255)
                                          gTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGgtg                                                           <  2:241950/100‑1 (MQ=255)
                                           ttttCCACTTCCGTGGTTGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGgtg                                                           <  2:3189602/99‑1 (MQ=255)
                                              tCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATc                                                       >  2:1841704/1‑100 (MQ=255)
                                               ccACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCTTGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATcc                                                      >  2:2556650/1‑100 (MQ=255)
                                                cACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATc                                                       >  2:3900374/1‑98 (MQ=255)
                                                cACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATccc                                                     >  2:1884524/1‑100 (MQ=255)
                                                    tCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCccgccg                                                 >  1:1130474/1‑100 (MQ=255)
                                                     ccGTGGTGGTGGTCAGTTGGCCGCCTTTTTTTTTTTTTTTTATCAGCACAGGTTGCAGCTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCgg                                                >  2:1955851/1‑100 (MQ=255)
                                                       gtggtggtgGTCAGTTGGCCGCCTTTTTCCTTGCCTGTAATCATCACAGGTTTCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCggg                                               <  2:3038607/99‑1 (MQ=255)
                                                        tggtggtggTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGcc                                             <  2:4035499/100‑1 (MQ=255)
                                                        tggtggtggTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGcc                                             <  2:159200/100‑1 (MQ=255)
                                                         ggtggtggtCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGcc                                             <  2:2916819/99‑1 (MQ=255)
                                                          gtggtggtCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTg                                           >  1:2426190/1‑100 (MQ=255)
                                                          gtggtggtCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTg                                           <  1:2256700/100‑1 (MQ=255)
                                                            ggtggtCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGaa                                         >  1:319737/1‑100 (MQ=255)
                                                             gtggtCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAAc                                        >  1:2220907/1‑100 (MQ=255)
                                                             gtggtCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAAc                                        >  2:1418922/1‑100 (MQ=255)
                                                              tggtCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAAcc                                       <  2:784657/100‑1 (MQ=255)
                                                               ggtCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAAccc                                      >  2:3552213/1‑100 (MQ=255)
                                                               ggtCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAAccc                                      >  1:3323953/1‑100 (MQ=255)
                                                               ggtCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAAccc                                      >  1:221244/1‑100 (MQ=255)
                                                               ggtCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAAccc                                      >  1:489929/1‑100 (MQ=255)
                                                                 tCAGTTGGCCGCCTTTTTCCATGCCGGTCATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCACGCCGGGCCTGAACCCAg                                    >  1:3228586/1‑100 (MQ=255)
                                                                 tCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAg                                    <  1:2678002/100‑1 (MQ=255)
                                                                  cAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAgg                                   >  2:3775669/1‑100 (MQ=255)
                                                                  cAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAgg                                   <  1:3893791/100‑1 (MQ=255)
                                                                   aGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGa                                  >  1:1560387/1‑100 (MQ=255)
                                                                     ttGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCCGCGGTGTATCCCGCCGGGCCTGAACCCAGGATa                                <  2:2691158/100‑1 (MQ=255)
                                                                       agacgccTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAg                              >  2:2823251/4‑100 (MQ=255)
                                                                        gccgccTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGc                             >  1:3146863/1‑100 (MQ=255)
                                                                        gccgccTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGc                             <  1:1416289/100‑1 (MQ=255)
                                                                        gccgccTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGc                             >  1:1292495/1‑100 (MQ=255)
                                                                         ccgccTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGGGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGGACCCAGGATAAGCa                            >  1:2056704/1‑100 (MQ=255)
                                                                         ccgccTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCa                            >  1:365413/1‑100 (MQ=255)
                                                                         ccgccTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCa                            >  2:3057834/1‑100 (MQ=255)
                                                                          cgccTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAg                           >  1:789815/1‑100 (MQ=255)
                                                                              tttttCCATGCCGGTAATCAGCACAGGTGGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTa                       <  2:2891860/100‑1 (MQ=255)
                                                                               ttttCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTAc                      <  1:1127548/100‑1 (MQ=255)
                                                                                 ttCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACtg                    <  2:4092543/100‑1 (MQ=255)
                                                                                 ttCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCCGAACCCAGGATAAGCAGTTTACtg                    <  2:2148578/100‑1 (MQ=255)
                                                                                  tCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTAc                      >  2:1977469/1‑97 (MQ=255)
                                                                                     aTGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGtt                <  1:591778/100‑1 (MQ=255)
                                                                                      tGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGtt                >  2:1188208/1‑99 (MQ=255)
                                                                                       gCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGTTTg              <  2:1308919/100‑1 (MQ=255)
                                                                                          ggTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGTTTGGTc           <  1:3662053/100‑1 (MQ=255)
                                                                                          ggTAATCAGAACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGTTTGGt            <  1:908600/99‑1 (MQ=255)
                                                                                           gTAATCAGCACAGGTTGCACGCTGCCGCGCGCCCCGCAGACAGCAGCGCTATATCCCGCAGTGCCTGAACCCAGGATAAGGAGTTTACCGTGTTTgtt            <  2:2755714/98‑3 (MQ=255)
                                                                                              aTCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGTTTGGTCGTGc       >  1:2360231/1‑100 (MQ=255)
                                                                                               tCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGTTTGGTCGTGcc      <  1:1958701/100‑1 (MQ=255)
                                                                                               tCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGTTTGGCCGTGcc      <  1:3055676/100‑1 (MQ=255)
                                                                                                cAGCACAGGTTGCAGTTTGGCGCGCGCCGCTTAGTCAGCAGCGGTGTAGCCCGCCGGGCCTGAACCCAGGATACGCTGTTTACTGTGTTTGGTCGTGcc      <  1:463876/99‑1 (MQ=255)
                                                                                                cAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGTTTGGTCGTGccc     >  1:3585620/1‑100 (MQ=255)
                                                                                                 aGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGTTTGGTCGTGCCCa    <  2:232741/100‑1 (MQ=255)
                                                                                                 aGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGTTTGGTCGTGCCCa    >  2:2139928/1‑100 (MQ=255)
                                                                                                  gCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGTTTGGTCGTGCCCAt   >  1:3319517/1‑100 (MQ=255)
                                                                                                  gCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGTTTGGTCGTGCCCAt   <  1:62112/100‑1 (MQ=255)
                                                                                                   cacaGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTTAACCCAGGATAAGCAGTTTACTGTGTTTGGTCGTGCCCATg  <  2:3082215/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CATTAATAACGGACCGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGTTTGGTCGTGCCCATG  >  NC_000913/931853‑932051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: