Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 933,387 C→T 25.1% A55V (GCC→GTC)  ftsK → DNA translocase at septal ring sorting daughter chromsomes

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913933,3870CT25.1% 273.1 / 84.5 151A55V (GCC→GTC) ftsKDNA translocase at septal ring sorting daughter chromsomes
Reads supporting (aligned to +/- strand):  ref base C (59/53);  new base T (18/20);  total (77/74)
Fisher's exact test for biased strand distribution p-value = 5.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 153 total aligned reads displayed.

TTCTGGAGGCGTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCC  >  NC_000913/933288‑933483
                                                                                                   |                                                                                                
ttCTGGAGGCGTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGc                                                                                                  <  1:977179/100‑1 (MQ=255)
    ggAGGCGTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTgn                                                                                              <  2:920414/100‑2 (MQ=255)
    ggAGGCGTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTg                                                                                               >  2:3891072/1‑99 (MQ=255)
    gcagtccttGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAATCTTTAACCTTTCGGTCCCCAGTTGTTCGCAAACGGCCTgg                                                                                              <  2:2533591/93‑1 (MQ=255)
     gAGGCGTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGc                                                                                             <  2:2964028/100‑1 (MQ=255)
     gAGGCGTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGc                                                                                             <  1:2431156/100‑1 (MQ=255)
      aGGCGTTGCTGATCCTTCTTGTCCTGTTTGTCGTCTGGTTGATGTCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGc                                                                                             <  2:1495077/99‑1 (MQ=255)
        gCGTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCATACGGCCTGGCATg                                                                                          <  1:2785814/100‑1 (MQ=255)
        gCGTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATg                                                                                          <  2:3055091/100‑1 (MQ=255)
         cGTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGa                                                                                         >  2:3652668/1‑100 (MQ=255)
          gTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGaa                                                                                        <  1:3622915/100‑1 (MQ=255)
          gTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGaa                                                                                        >  2:468772/1‑100 (MQ=255)
           ttGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAAc                                                                                       <  1:1699294/100‑1 (MQ=255)
           ttGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAAc                                                                                       <  1:850746/100‑1 (MQ=255)
            tGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAAcc                                                                                      >  1:2265636/1‑100 (MQ=255)
            tGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAAcc                                                                                      <  2:728961/100‑1 (MQ=255)
            tGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAAcc                                                                                      >  1:3641153/1‑100 (MQ=255)
            tGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAAcc                                                                                      <  1:1356685/100‑1 (MQ=255)
            tGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAAcc                                                                                      <  1:1356533/100‑1 (MQ=255)
               tGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTAt                                                                                   >  2:807688/1‑100 (MQ=255)
                gATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATc                                                                                  >  1:1886448/1‑100 (MQ=255)
                  tCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCa                                                                                <  1:382208/100‑1 (MQ=255)
                   ccTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTCTCCa                                                                                >  2:1267620/1‑99 (MQ=255)
                   ccTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCAt                                                                               >  1:2379074/1‑100 (MQ=255)
                      tATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAAt                                                                            <  1:2232969/100‑1 (MQ=255)
                      tATTGTCCTGTTTGCCGTCTGGTTGAGGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAAt                                                                            <  1:75748/100‑1 (MQ=255)
                         tGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTa                                                                         >  1:2328469/1‑100 (MQ=255)
                          gTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAg                                                                        <  1:3981286/100‑1 (MQ=255)
                          gTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCAAGCTGGTCGCAAAAGGTCTGGCATGAACCTATCCAAAATTTAg                                                                        >  1:4117503/1‑100 (MQ=255)
                           tCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAgg                                                                       <  1:2831682/100‑1 (MQ=255)
                           tCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAgg                                                                       <  2:919936/100‑1 (MQ=255)
                           tCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAgg                                                                       <  2:1278084/100‑1 (MQ=255)
                            ccTGTTTGCGGTGTGGTTGATGGCTGCCTTACTCCGCTTTACCCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAgg                                                                       <  2:1427353/99‑1 (MQ=255)
                             cTGTGTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGCCCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAACTTAGGTg                                                                     <  2:3532301/100‑1 (MQ=255)
                               gTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTggg                                                                   >  1:807307/1‑100 (MQ=255)
                                tttGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGa                                                                  <  1:3679813/100‑1 (MQ=255)
                                   gCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCATCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGc                                                               >  2:2538619/1‑100 (MQ=255)
                                     cGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGccc                                                             <  1:985396/100‑1 (MQ=255)
                                      gTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCg                                                            <  1:2560263/100‑1 (MQ=255)
                                         tGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTg                                                         >  2:3624113/1‑100 (MQ=255)
                                         tGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTg                                                         >  2:2590017/1‑100 (MQ=255)
                                         tGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTg                                                         <  1:1934268/100‑1 (MQ=255)
                                          ggTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGc                                                        <  1:1149851/100‑1 (MQ=255)
                                          ggTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGc                                                        <  2:375942/100‑1 (MQ=255)
                                           gTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCg                                                       >  1:3525987/1‑100 (MQ=255)
                                           gTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCg                                                       >  1:3378789/1‑100 (MQ=255)
                                           gTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCg                                                       <  1:3176793/100‑1 (MQ=255)
                                           gTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCg                                                       >  1:3094843/1‑100 (MQ=255)
                                             tGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTg                                                     <  1:3641791/100‑1 (MQ=255)
                                             tGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTg                                                     <  1:1544745/100‑1 (MQ=255)
                                              gATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGACGCCCGGTGCGtcg                                                    <  1:2806432/100‑3 (MQ=255)
                                               aTGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGAGGGATGCCCGGTg                                                         >  2:1004215/1‑94 (MQ=255)
                                                tGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTGGGTGGGATGCCCGGTGCGTGGtt                                                  >  2:3127864/1‑100 (MQ=255)
                                                tGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGtt                                                  <  2:3185580/100‑1 (MQ=255)
                                                tGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGtt                                                  >  1:2922598/1‑100 (MQ=255)
                                                  gCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTgg                                                >  2:3380691/1‑100 (MQ=255)
                                                    tGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCa                                              <  2:2737125/100‑1 (MQ=255)
                                                    tGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCa                                              <  1:2874796/100‑1 (MQ=255)
                                                      ccTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGa                                            >  1:3568643/1‑100 (MQ=255)
                                                      ccTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGa                                            <  1:1817682/100‑1 (MQ=255)
                                                       cTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGAt                                           >  2:2147852/1‑100 (MQ=255)
                                                       cTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGAt                                           >  1:432904/1‑100 (MQ=255)
                                                           cTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGc                                       >  2:1779609/1‑100 (MQ=255)
                                                              aGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGTCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATaa                                         >  1:2973998/1‑94 (MQ=255)
                                                              aGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGACTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGt                                    <  2:1857418/100‑1 (MQ=255)
                                                               gCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGtt                                   >  2:1958375/1‑100 (MQ=255)
                                                               gCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGtt                                   >  2:1699044/1‑100 (MQ=255)
                                                               gCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGtt                                   >  1:3195497/1‑100 (MQ=255)
                                                                cTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGCCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTc                                  >  2:805625/1‑100 (MQ=255)
                                                                 tttAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCt                                 <  1:3667347/100‑1 (MQ=255)
                                                                 tttAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCt                                 <  2:1892202/100‑1 (MQ=255)
                                                                   tAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCttt                               >  1:4094914/1‑100 (MQ=255)
                                                                   tAACACTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGAACGTGGATGGAAGATACGATgatat                                 >  2:160747/1‑94 (MQ=255)
                                                                     aCCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTAt                             >  1:248657/1‑100 (MQ=255)
                                                                     aCCCTTAGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTAt                             >  2:1177726/1‑100 (MQ=255)
                                                                      cgcTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTAtt                            >  1:2805132/3‑100 (MQ=255)
                                                                      cccTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTa                              >  2:1705184/1‑98 (MQ=255)
                                                                      cccTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTAt                             <  2:1052118/99‑1 (MQ=255)
                                                                       ccTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGCACCTATCCATAATTTAGGTGGGCTGCCCGGTGCGTGTGTGGCAGATACGCTGTTctttcttt                           >  2:3769872/1‑100 (MQ=255)
                                                                       ccTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTAttt                           <  1:312803/100‑1 (MQ=255)
                                                                       ccTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTAttt                           >  1:3444505/1‑100 (MQ=255)
                                                                          tCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTg                        >  2:1080067/1‑100 (MQ=255)
                                                                           cGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTgg                       <  2:260327/100‑1 (MQ=255)
                                                                             gACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGc                      >  2:2243953/1‑99 (MQ=255)
                                                                             gACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCg                     <  2:2438825/100‑1 (MQ=255)
                                                                                 ccAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGAt                 >  2:1597726/1‑100 (MQ=255)
                                                                                 ccAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGAt                 >  2:740479/1‑100 (MQ=255)
                                                                                    gCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGc              <  2:2148507/100‑1 (MQ=255)
                                                                                    gCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGc              >  2:3849288/1‑100 (MQ=255)
                                                                                      tGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCtt            <  1:841702/100‑1 (MQ=255)
                                                                                        gTCGCAAACGGTCGGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTac          >  1:2779081/1‑100 (MQ=255)
                                                                                        gTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTac          >  2:1098654/1‑100 (MQ=255)
                                                                                           gCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACAcc       <  1:1098465/100‑1 (MQ=255)
                                                                                            cAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCa      >  1:1928716/1‑100 (MQ=255)
                                                                                            cAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCa      >  2:95708/1‑100 (MQ=255)
                                                                                              aaCGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCAtt    >  1:3948784/1‑100 (MQ=255)
                                                                                              aaCGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCAt     <  2:2003388/99‑1 (MQ=255)
                                                                                                cGGTCTGGCATGAACCTATCCATAACTTAGGTGGGATGCCCGGTGCGCGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTcc  >  2:2449768/1‑100 (MQ=255)
                                                                                                cGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTcc  >  2:499909/1‑100 (MQ=255)
                                                                                                cGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGCCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTcc  <  2:2288872/100‑1 (MQ=255)
                                                                                                   |                                                                                                
TTCTGGAGGCGTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCC  >  NC_000913/933288‑933483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: