Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 975,083 T→A 24.9% D255E (GAT→GAA mukF → chromosome condensin MukBEF, kleisin‑like subunit, binds calcium

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913975,0830TA24.9% 243.6 / 83.1 134D255E (GAT→GAAmukFchromosome condensin MukBEF, kleisin‑like subunit, binds calcium
Reads supporting (aligned to +/- strand):  ref base T (47/51);  new base A (17/16);  total (65/69)
Fisher's exact test for biased strand distribution p-value = 8.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 135 total aligned reads displayed.

GGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACCG  >  NC_000913/974984‑975180
                                                                                                   |                                                                                                 
ggAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATGTCGTCGATCGTCTGGTGTTCGAt                                                                                                   >  1:2248616/1‑100 (MQ=255)
 gAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATc                                                                                                  <  2:3524991/100‑1 (MQ=255)
  aaGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCt                                                                                                 <  1:1142343/100‑1 (MQ=255)
  aaGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATAACGATGTGCATTTCATCGATCGTCTGGTGTTCGAACt                                                                                                 >  2:2788365/1‑100 (MQ=255)
    gCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGc                                                                                               <  1:3987264/100‑1 (MQ=255)
     cGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCa                                                                                              <  1:531393/100‑1 (MQ=255)
     cGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCa                                                                                              <  1:1000962/100‑1 (MQ=255)
     cGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCa                                                                                              >  1:2234300/1‑100 (MQ=255)
      ggCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCag                                                                                             <  1:1101357/100‑1 (MQ=255)
       gCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCaga                                                                                            >  2:589303/1‑100 (MQ=255)
       gCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCaga                                                                                            <  1:1218600/100‑1 (MQ=255)
        cAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCagag                                                                                           <  2:468223/100‑1 (MQ=255)
        cAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCagag                                                                                           <  2:2774773/100‑1 (MQ=255)
        cAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCaga                                                                                            >  2:3702050/1‑99 (MQ=255)
        cAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCagag                                                                                           <  2:3354682/100‑1 (MQ=255)
         aGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGTCGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGc                                                                                          <  2:2642249/100‑1 (MQ=255)
          ggCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCa                                                                                         >  2:2086162/1‑100 (MQ=255)
           gCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCaa                                                                                        >  1:3569385/1‑100 (MQ=255)
           gCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCaa                                                                                        >  2:2263550/1‑100 (MQ=255)
               cAAATTGCATGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTc                                                                                    <  2:3749436/100‑1 (MQ=255)
                  atggCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGAt                                                                                 <  2:206855/97‑1 (MQ=255)
                  aTTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGAt                                                                                 <  1:1694860/100‑1 (MQ=255)
                  aTTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGAt                                                                                 >  2:2104005/1‑100 (MQ=255)
                   ttGCAGGCTAATCTGTTGCGCATTCAGGCTGCCACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATc                                                                                <  2:3085541/100‑1 (MQ=255)
                   ttGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGACCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATc                                                                                >  2:1700497/1‑100 (MQ=255)
                    tGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCg                                                                               <  2:1917711/100‑1 (MQ=255)
                     gCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCg                                                                               >  2:4100605/1‑99 (MQ=255)
                      cAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGta                                                                             <  1:2261022/100‑1 (MQ=255)
                       aGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGtat                                                                            >  2:4061221/1‑100 (MQ=255)
                       aGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGtat                                                                            <  1:4061248/100‑1 (MQ=255)
                          cTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGtattat                                                                         >  2:2481598/1‑100 (MQ=255)
                           tAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGtattat                                                                         >  2:2360594/1‑99 (MQ=255)
                              tCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGt                                                                     <  1:2645265/100‑1 (MQ=255)
                                 gTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTgg                                                                  <  2:2145095/100‑1 (MQ=255)
                                  ttGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTggg                                                                 <  2:2132617/100‑1 (MQ=255)
                                   tGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTgggg                                                                <  1:1270868/100‑1 (MQ=255)
                                   tGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTgggg                                                                >  1:4103751/1‑100 (MQ=255)
                                   tGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTgggg                                                                >  1:1624921/1‑100 (MQ=255)
                                   tGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTgggg                                                                <  1:2622430/100‑1 (MQ=255)
                                   tGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTgggg                                                                <  1:950161/100‑1 (MQ=255)
                                      gcATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTgggg                                                                <  2:3527867/97‑1 (MQ=255)
                                      acATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGGCTGGAGATCGAGCTGCAGAGCAAACTCGATCGGATTATCAGTTgggg                                                                >  2:2460932/2‑97 (MQ=255)
                                        aTTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGc                                                           <  1:213471/100‑1 (MQ=255)
                                        aTTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGc                                                           >  1:3122879/1‑100 (MQ=255)
                                           cAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAAt                                                        >  1:384004/1‑100 (MQ=255)
                                            aGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATc                                                       <  2:3997999/100‑1 (MQ=255)
                                            aGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATc                                                       <  1:590184/100‑1 (MQ=255)
                                                tGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATc                                                   >  1:3633993/1‑100 (MQ=255)
                                                tGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATc                                                   <  1:3846127/100‑1 (MQ=255)
                                                 gCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCg                                                  >  2:2933518/1‑100 (MQ=255)
                                                  cgacgaTGACCCATGACGATCTGCATTTCTTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCg                                                  <  2:826522/99‑1 (MQ=255)
                                                  cgacgaTGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGa                                                 >  2:3439925/1‑100 (MQ=255)
                                                  cgacgaTGACCCATGACGATCTGCATTTCGTCGACCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGa                                                 >  1:3297870/1‑100 (MQ=255)
                                                     cgaTGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACtt                                              >  2:1924847/1‑100 (MQ=255)
                                                        tGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTtgtg                                           <  1:738584/100‑1 (MQ=255)
                                                         gACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTgg                                          <  2:3612967/100‑1 (MQ=255)
                                                          aCCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTtgtg                                           >  2:1264361/1‑98 (MQ=255)
                                                          aCCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGa                                         >  1:4131243/1‑100 (MQ=255)
                                                           cccATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGAt                                        >  2:1422489/1‑100 (MQ=255)
                                                           cccATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGAt                                        >  2:489741/1‑100 (MQ=255)
                                                           cccATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGAt                                        <  2:1685381/100‑1 (MQ=255)
                                                           cccATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGAt                                        >  1:2077822/1‑100 (MQ=255)
                                                           cccATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGAt                                        <  2:2015900/100‑1 (MQ=255)
                                                           cccATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGAt                                        <  2:4131216/100‑1 (MQ=255)
                                                           cccATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGAt                                        >  1:3972259/1‑100 (MQ=255)
                                                             cATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTg                                      >  1:174560/1‑100 (MQ=255)
                                                              aTGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTTCAGAGCAAACTCGATCGTATTATCATTTGGGGCCAGCAATCCATCGACTTGTGGATTgg                                     <  1:249199/100‑1 (MQ=255)
                                                              aTGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTgg                                     <  2:1284661/100‑1 (MQ=255)
                                                                gACGATCTGCATTTCGTCGATGGTTTGGTGTTCGAGCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTgg                                     <  2:1279073/98‑1 (MQ=255)
                                                                gACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCt                                   <  1:2503168/100‑1 (MQ=255)
                                                                   gATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTGTGAGTTGGGGCCAGCAATCAATCGACTTGTGGATTGGCTAc                                 >  2:2926874/1‑99 (MQ=255)
                                                                   gATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACg                                >  2:1483689/1‑100 (MQ=255)
                                                                   gATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACg                                >  1:3331891/1‑100 (MQ=255)
                                                                     tCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGTTACGAc                              <  1:2768014/100‑1 (MQ=255)
                                                                      cTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGAcc                             >  1:3170556/1‑100 (MQ=255)
                                                                      cTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGAcc                             <  1:643089/100‑1 (MQ=255)
                                                                       tGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCg                            <  1:2655978/100‑1 (MQ=255)
                                                                       tGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCg                            >  2:2980848/1‑100 (MQ=255)
                                                                        gCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGc                           >  2:161191/1‑100 (MQ=255)
                                                                             tcgtcgATCGTCTGGTGTTCGAACGGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGt                      <  2:142974/100‑1 (MQ=255)
                                                                               gtcgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTac                    <  2:960705/100‑1 (MQ=255)
                                                                               gtcgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTac                    >  1:245062/1‑100 (MQ=255)
                                                                                  gATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTAcaca                 >  1:2667464/1‑100 (MQ=255)
                                                                                   aTCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCAAGCAATCCATCGACTTGTGTATTGGCTACGACCGCCACGAAcaca                 >  2:544967/1‑99 (MQ=255)
                                                                                    tCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACaaa               <  1:3168947/100‑1 (MQ=255)
                                                                                     cGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAAt              >  1:3501183/1‑100 (MQ=255)
                                                                                     cGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAAt              >  1:670179/1‑100 (MQ=255)
                                                                                     cGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAAt              >  2:278753/1‑100 (MQ=255)
                                                                                        cTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTa           >  1:1893134/1‑100 (MQ=255)
                                                                                           gtgtTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGAGTGGCTACGACCGCCACGTACAGAAATTTATTc        <  2:3332089/100‑1 (MQ=255)
                                                                                            tgtTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCg       <  2:3753113/100‑1 (MQ=255)
                                                                                             gtTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGt      >  1:1234544/1‑100 (MQ=255)
                                                                                             gtTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGt      >  2:3195951/1‑100 (MQ=255)
                                                                                             gtTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGt      >  2:526210/1‑100 (MQ=255)
                                                                                              ttCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTa     <  1:589308/100‑1 (MQ=255)
                                                                                               tCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTAc    <  2:2234287/100‑1 (MQ=255)
                                                                                                cGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTAcc   >  1:3733837/1‑100 (MQ=255)
                                                                                                cGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGt      >  1:978150/1‑97 (MQ=255)
                                                                                                 gATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCgagatc  >  2:1181542/1‑95 (MQ=255)
                                                                                                 gATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACcg  <  1:3312656/100‑1 (MQ=255)
                                                                                                   |                                                                                                 
GGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACCG  >  NC_000913/974984‑975180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: