BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,100,233	G→A	100%	intergenic (+39/‑63)	ycdY → / → ycdZ	redox enzyme maturation protein (REMP) chaperone for YcdX/DUF1097 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,100,233	0	G	A	100.0%	386.6 / ‑6.7	115	intergenic (+39/‑63)	ycdY/ycdZ	redox enzyme maturation protein (REMP) chaperone for YcdX/DUF1097 family inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (56/58); minor base T (0/1); total (56/59)

Only 100 of 127 total aligned reads displayed.

TAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACA  >  NC_000913/1100136‑1100328

tAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttatt                                                                                               <  1:738723/100‑1 (MQ=255)

 aaCCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattg                                                                                              <  2:2202167/100‑1 (MQ=255)

 aaCCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattg                                                                                              >  1:3771373/1‑100 (MQ=255)

 aaCCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattg                                                                                              <  2:3365812/100‑1 (MQ=255)

 aaCCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattg                                                                                              <  1:1803533/100‑1 (MQ=255)

  aCCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattgt                                                                                             <  1:18462/100‑1 (MQ=255)

    ccGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATACTttattgttg                                                                                           >  2:1658386/1‑100 (MQ=255)

    ccGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattgttg                                                                                           <  2:1759240/100‑1 (MQ=255)

    ccGCGATGCCATTAGTGCAATGTGGAACTACCTGGAATAAGACTCTGAAGAATAATTATGATCTACGTCACTCATAACTGCATCGGATAATttattgttg                                                                                           <  2:3326467/100‑1 (MQ=255)

      gcgATGCCATTAGTGCAATGTGGGACGAGATGGAAGAAGATTCTGAAGAATAAGTGTTATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCa                                                                                         <  2:1499319/100‑1 (MQ=255)

       cgATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCAt                                                                                        <  2:1883192/100‑1 (MQ=255)

       cgATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCAt                                                                                        <  1:1162439/100‑1 (MQ=255)

        gATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATa                                                                                       <  1:3170397/100‑1 (MQ=255)

        gATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATa                                                                                       >  1:3473984/1‑100 (MQ=255)

            ccATTAGTGCAATGTGGGACGAGCTGGCAGAAGATTCTGAAGAATAAATGTGCTCTACGTCACTCAGCACTGCAACGGATAACttatt                                                                                               >  2:3822351/1‑88 (MQ=255)

             cATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtg                                                                                  >  1:2498720/1‑100 (MQ=255)

             cATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtg                                                                                  >  2:1782621/1‑100 (MQ=255)

             cATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtg                                                                                  >  2:1931332/1‑100 (MQ=255)

             cATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtg                                                                                  >  1:1627087/1‑100 (MQ=255)

             cATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtg                                                                                  >  1:1030531/1‑100 (MQ=255)

                 aGTGCAATGTGTGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATTTACGGCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtgtgtg                                                                              <  2:3583665/100‑1 (MQ=255)

                 aGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtgtgtg                                                                              <  1:3092767/100‑1 (MQ=255)

                 aGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtgtgtg                                                                              <  1:332472/100‑1 (MQ=255)

                  gTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGc                                                                             >  1:3626357/1‑100 (MQ=255)

                  gTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGc                                                                             >  1:2942352/1‑100 (MQ=255)

                    gCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTc                                                                           >  2:3373202/1‑100 (MQ=255)

                    gCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTc                                                                           >  1:3092395/1‑100 (MQ=255)

                     cAATGTGGGACGAGCTGGATGAAGATTCCGAAGACTAAGTGTGAGCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGc                                                                             <  2:2786134/97‑1 (MQ=255)

                       aTGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGAt                                                                        <  2:4045934/100‑1 (MQ=255)

                        tgtgGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGAtc                                                                       <  2:1504726/100‑1 (MQ=255)

                         gtgGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGAtct                                                                      <  1:3734787/100‑1 (MQ=255)

                         gtgGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGAtct                                                                      <  2:941745/100‑1 (MQ=255)

                          tgGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGAtctc                                                                     >  1:2705069/1‑100 (MQ=255)

                           gggACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCtca                                                                    >  2:448815/1‑100 (MQ=255)

                           gggACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCGACGTCACTCATAACTGCAATGGATAATTTATTGTTGCAGAAAATGTGTGCTCGATCtca                                                                    >  2:2830487/1‑100 (MQ=255)

                            ggACGAGCTGGAAGAAGATTCTGAAGTATAAGTGTTATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCtcat                                                                   <  2:2379768/100‑1 (MQ=255)

                            ggACGAGCTGGAAGAAGATTCTGAAGAATAAGTTTAATCTATGTCACTCAGAACTGCATCGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCtcat                                                                   <  2:2565531/100‑1 (MQ=255)

                             gACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCtcatt                                                                  <  2:470370/100‑1 (MQ=255)

                              aCGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCtcattc                                                                 <  1:536076/100‑1 (MQ=255)

                               cGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCtcattca                                                                >  2:1430849/1‑100 (MQ=255)

                                gAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCtcattcat                                                               >  1:2730993/1‑100 (MQ=255)

                                 aGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATg                                                              >  1:1447066/1‑100 (MQ=255)

                                  gCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATgg                                                             <  1:3909811/100‑1 (MQ=255)

                                  gCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATGGTTGCATAAAATGTGTGCTCGATCTCATTCATgg                                                             <  1:1210910/100‑1 (MQ=255)

                                   cTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGc                                                            <  2:1496875/100‑1 (MQ=255)

                                     ggAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCcg                                                          >  1:181533/1‑100 (MQ=255)

                                     ggAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCcg                                                          >  2:2212415/1‑100 (MQ=255)

                                     ggAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCcg                                                          <  2:3514178/100‑1 (MQ=255)

                                          aagaTTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGttt                                                     <  2:3043515/100‑1 (MQ=255)

                                           agaTTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGtttt                                                    >  1:1977566/1‑100 (MQ=255)

                                           agaTTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGtttt                                                    >  1:161971/1‑100 (MQ=255)

                                           agaTTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGtttt                                                    >  1:2614525/1‑100 (MQ=255)

                                              ttCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTg                                                 <  2:40579/100‑1 (MQ=255)

                                              ttCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTg                                                 >  2:1864952/1‑100 (MQ=255)

                                              ttCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTg                                                 >  2:3741401/1‑100 (MQ=255)

                                                  gaagaaTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTAt                                             <  1:4012559/100‑1 (MQ=255)

                                                  gaagaaTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTAt                                             >  1:2205191/1‑100 (MQ=255)

                                                    agaaTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCa                                           >  2:2093584/1‑100 (MQ=255)

                                                        tAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGGTCTCATTCATGGCCGCGTTTTCTGCTATCATgcg                                       >  2:194743/1‑100 (MQ=255)

                                                        tAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATgcg                                       >  2:1013202/1‑100 (MQ=255)

                                                         aaGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATgcgc                                      <  1:1651356/100‑1 (MQ=255)

                                                          aGTGTGATTTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGcgcg                                     >  2:3028140/1‑100 (MQ=255)

                                                          aGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATgcgcn                                     <  2:38885/100‑2 (MQ=255)

                                                          aGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGcgcg                                     >  2:1115985/1‑100 (MQ=255)

                                                          aGTGTGATCTACGTCACTCATAACTGCAACGGATAATGTATTGCTGCATAAAATGTATGCTCGATCTCATTTATGGCCGCGTTTTCTGCTATCATGcgcg                                     >  2:849460/1‑100 (MQ=255)

                                                            tgtgATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGc                                   <  1:2566467/100‑1 (MQ=255)

                                                             gtgATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCa                                  <  2:2265119/100‑1 (MQ=255)

                                                              tgATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCAt                                 >  1:597140/1‑100 (MQ=255)

                                                               gATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATg                                <  2:628124/100‑1 (MQ=255)

                                                               gATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATg                                >  1:31336/1‑100 (MQ=255)

                                                                  cTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAAc                             <  1:4086885/100‑1 (MQ=255)

                                                                  cTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAAc                             <  1:1877658/100‑1 (MQ=255)

                                                                  cTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAAc                             <  1:242532/100‑1 (MQ=255)

                                                                   tACGTCACTCATTACTGGAACGGATAATTTATTGTTGCATTAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGTCATGaa                              <  2:2050622/98‑1 (MQ=255)

                                                                   tACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACa                            <  1:3615114/100‑1 (MQ=255)

                                                                   tACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCCCATTCATGACCGCGTTTTCTGCTACCATGCGCGGCATGaaa                             >  2:1119513/1‑98 (MQ=255)

                                                                    acttCACTCATAACTTCATCGGATAATTTTTTGTTGCATAAAATGTGTGCTCCATCTCATTCATTTCCTCGTTTTCTGCTATCATGCGCGGCATGAACAt                           <  2:973205/97‑1 (MQ=255)

                                                                       tCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACt                        >  2:3325109/1‑100 (MQ=255)

                                                                       tCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACt                        >  2:2906286/1‑100 (MQ=255)

                                                                       tCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACt                        <  1:3026226/100‑1 (MQ=255)

                                                                            cATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCTTTTTCTGCTATCATGCTCGGCATTAACATACGtctc                    >  2:2056440/1‑99 (MQ=255)

                                                                            cATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtctct                   <  2:2837665/100‑1 (MQ=255)

                                                                             aTAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtctctc                  <  1:3329283/100‑1 (MQ=255)

                                                                              tAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTctctct                 <  1:1389194/100‑1 (MQ=255)

                                                                               aaCTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTa                <  1:2098382/100‑1 (MQ=255)

                                                                                aCTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTAt               <  1:1115992/100‑1 (MQ=255)

                                                                                 cTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCCTTCATGGCCGCGTTTTCTGCTATCCTGCGCGGCATGACCATACTTCTCTCTCtt              >  1:3448440/1‑100 (MQ=255)

                                                                                 cTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTAtt              >  2:1729382/1‑100 (MQ=255)

                                                                                 cTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTAtt              <  2:236266/100‑1 (MQ=255)

                                                                                   gCAACGGATAATTTATTGTTGCATAAAATGTGTGTTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTCCTCACTATTGc            <  2:2169031/100‑1 (MQ=255)

                                                                                   gCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGc            >  2:969062/1‑100 (MQ=255)

                                                                                   gCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCGGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGc            <  1:117143/100‑1 (MQ=255)

                                                                                     aaCGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCaa          >  2:2413265/1‑100 (MQ=255)

                                                                                      aCGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAAt         <  2:4111320/100‑1 (MQ=255)

                                                                                      aCGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAAt         >  1:2963570/1‑100 (MQ=255)

                                                                                        ggATTATTGATTGTTGCATAATATGTGTGCTCGTGGTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCGCTCTAGTGCAATc        <  2:3571055/99‑1 (MQ=255)

                                                                                         gATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATcac      <  2:3450790/100‑1 (MQ=255)

                                                                                          aTAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATcacn     <  2:558250/100‑2 (MQ=255)

                                                                                            aaTTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCTGCATGAACATACTTCTCTCTATTGCAATCacaac   <  1:4027138/100‑1 (MQ=255)

                                                                                             aTTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCacaaca  >  2:312603/1‑100 (MQ=255)

TAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACA  >  NC_000913/1100136‑1100328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`