Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,105,806 T→C 51.7% intergenic (+81/‑14) ymdA → / → ymdB uncharacterized protein/O‑acetyl‑ADP‑ribose deacetylase; RNase III inhibitor during cold shock; putative cardiolipin synthase C regulatory subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,105,8060TC51.7% 23.9 / 170.3 138intergenic (+81/‑14)ymdA/ymdBuncharacterized protein/O‑acetyl‑ADP‑ribose deacetylase; RNase III inhibitor during cold shock; putative cardiolipin synthase C regulatory subunit
Reads supporting (aligned to +/- strand):  ref base T (32/35);  new base C (44/27);  total (76/62)
Fisher's exact test for biased strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 139 total aligned reads displayed.

ATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGCTAATCCGTCATTAA  >  NC_000913/1105711‑1105904
                                                                                               |                                                                                                  
aTCGAATATAGCTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTaa                                                                                                >  2:60929/1‑100 (MQ=255)
aTCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTaa                                                                                                <  1:1792632/100‑1 (MQ=255)
aTCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTaa                                                                                                >  1:2591969/1‑100 (MQ=255)
 tCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAag                                                                                               <  2:3931327/100‑1 (MQ=255)
 tCGAATATAACTAAGGTTCTGAACAAATCACGTTGCTGAGCAGAAAAATGAAAATAAAACAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAag                                                                                               <  2:3579403/100‑1 (MQ=255)
   gAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAagga                                                                                             >  1:2620077/1‑100 (MQ=255)
   gAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGAAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAagga                                                                                             >  1:836279/1‑100 (MQ=255)
      tataACTAAGTTTCTGAACAACTCACGTTGCTGAGCGGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAAAAAGAAGGCGTACCGAGGc                                                                                          >  1:3698560/1‑100 (MQ=255)
      tataACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGc                                                                                          <  1:1353849/100‑1 (MQ=255)
      tataACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGc                                                                                          <  2:2200281/100‑1 (MQ=255)
        taACTAAGTTTCTGAACAACTCACGTTGCTGATCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGt                                                                                        <  1:3450586/100‑1 (MQ=255)
         aaCTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGtt                                                                                       >  2:3880870/1‑100 (MQ=255)
           cTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTAt                                                                                     <  2:3096845/100‑1 (MQ=255)
           cTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTAt                                                                                     >  1:3920522/1‑100 (MQ=255)
            tAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATg                                                                                    <  1:3047230/100‑1 (MQ=255)
            tAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATg                                                                                    <  1:311422/100‑1 (MQ=255)
            tAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATg                                                                                    <  2:3168120/100‑1 (MQ=255)
             aaGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGa                                                                                   >  1:3343200/1‑100 (MQ=255)
              aGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGaa                                                                                  >  1:2882817/1‑100 (MQ=255)
               gattCTGAACAACTCACTTTGCTGATCAGAAAAATACGATAAAACAAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGaaa                                                                                 <  2:1604215/98‑1 (MQ=255)
               gTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGaaa                                                                                 >  1:2000023/1‑100 (MQ=255)
                tttCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTCAGGAGGCGTTATGaaaa                                                                                >  1:1294395/1‑100 (MQ=255)
                 gtCTGAACAACTCACGTGGCTGAGCAGAAAAAGGCGAGTTAACCAAAAAGCGTGCTGTACACTGAAGAAACAAGAAGGTGTAAGGAGGCGTTATGaaaa                                                                                <  2:3820551/98‑1 (MQ=255)
                  tCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAAcg                                                                              <  2:1139839/100‑1 (MQ=255)
                  tCTGAACAACTCACGTTGCTGAGCAGAAAAATACGATTTAACCAAAAAGCATGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAAcg                                                                              >  2:1431400/1‑100 (MQ=255)
                  tCTGAACAACTCACGTTGCAAAGAAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGaaaa                                                                                <  2:862400/98‑1 (MQ=255)
                   cTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAAcgc                                                                             <  2:903191/100‑1 (MQ=255)
                     gAACAACTCACGGGGCTGAGCAGAAAAATGCGAGTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGt                                                                           >  2:2250825/1‑100 (MQ=255)
                        caacTCACGTTGCTGAGCAGAAAAATGCGATTTCACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTAtt                                                                        >  2:2728362/1‑100 (MQ=255)
                        caacTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGAGTAtt                                                                        >  2:3841227/1‑100 (MQ=255)
                         aacTAACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGATGTAAACTAAAGAAACAAGAATGCGTAAGTAGGCGTTATGAAAACGGGTATTc                                                                       >  2:3949568/1‑100 (MQ=255)
                             cACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCAtgt                                                                   >  2:1572047/1‑100 (MQ=255)
                             cACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCAtgt                                                                   >  1:2303510/1‑100 (MQ=255)
                             cACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCAtgt                                                                   <  1:2818234/100‑1 (MQ=255)
                             cACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCAtgt                                                                   <  1:999056/100‑1 (MQ=255)
                               cGTTGCTGAGCATAAAAATGCGATTTAACAAAAAAGCCTGCGGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCAtgtt                                                                  >  2:1365003/1‑99 (MQ=255)
                                 ttGCTGAGCAAAAAAATGCGATTTAACCAAAAAGCCTGATGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCAtgttg                                                                 <  1:1576468/98‑1 (MQ=255)
                                  tGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGc                                                              <  1:2540884/100‑1 (MQ=255)
                                  tGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGc                                                              >  1:54182/1‑100 (MQ=255)
                                   gCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCa                                                             >  2:2078556/1‑100 (MQ=255)
                                    cTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAg                                                            <  1:97266/100‑1 (MQ=255)
                                    cTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAg                                                            <  1:2126124/100‑1 (MQ=255)
                                    cTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAg                                                            <  1:2126187/100‑1 (MQ=255)
                                      gAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAggg                                                          <  1:754011/100‑1 (MQ=255)
                                       aGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAGACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGt                                                         <  2:2621286/100‑1 (MQ=255)
                                       aGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGt                                                         <  2:1097548/100‑1 (MQ=255)
                                        gCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTg                                                        >  2:3545149/1‑100 (MQ=255)
                                         cAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTg                                                        >  1:4057055/1‑99 (MQ=255)
                                           gAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGata                                                     >  1:3846184/1‑100 (MQ=255)
                                            aacaaTGCTATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGata                                                     <  2:3320484/96‑1 (MQ=255)
                                             aaaaTGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATAtt                                                   >  1:3395044/1‑100 (MQ=255)
                                              aaaTGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTa                                                  <  2:3363703/100‑1 (MQ=255)
                                               aaTGCGATTTAACCAAAAAGCCTGCTGTACACTTAACAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTAc                                                 <  1:2905867/100‑1 (MQ=255)
                                                aTGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTAcc                                                <  2:2921622/100‑1 (MQ=255)
                                                 tGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCa                                               >  1:3089962/1‑100 (MQ=255)
                                                 ngCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCa                                               >  2:3637852/2‑100 (MQ=255)
                                                   cGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCaag                                             >  1:3220836/1‑99 (MQ=255)
                                                    gATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGCGATAttcc                                                 >  2:3115619/1‑93 (MQ=255)
                                                     aTTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCACACt                                           >  1:1192557/1‑100 (MQ=255)
                                                     aTTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACt                                           >  2:3744099/1‑100 (MQ=255)
                                                      tttAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTg                                          >  2:3241838/1‑100 (MQ=255)
                                                         aaCCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGcc                                       >  1:2725958/1‑100 (MQ=255)
                                                         aaCCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGcc                                       >  1:2001427/1‑100 (MQ=255)
                                                          aCCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCg                                      >  1:710728/1‑100 (MQ=255)
                                                            cAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGtt                                    >  2:2498214/1‑100 (MQ=255)
                                                             aaaaaGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTg                                   <  1:2503668/100‑1 (MQ=255)
                                                              aaaaGCCTGCTGTACACTTAAGCAACAAGAACGTGTACGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTgn                                  <  2:2792269/100‑2 (MQ=255)
                                                               aaaGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGAt                                 <  1:3725809/100‑1 (MQ=255)
                                                                aaGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGAta                                <  1:2354695/100‑2 (MQ=255)
                                                                aaGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGAtg                                >  1:3048703/1‑100 (MQ=255)
                                                                 aGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGAt                                 <  2:1914190/98‑1 (MQ=255)
                                                                  gCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGAtgtg                              <  1:125685/100‑1 (MQ=255)
                                                                   ccTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGCGATATTACCAAACTGGCCGTTGATGTGa                             >  2:272233/1‑100 (MQ=255)
                                                                    ctgctgTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGAt                            <  1:1520693/100‑1 (MQ=255)
                                                                      gctgTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATtg                          >  2:3312644/1‑100 (MQ=255)
                                                                      gctgTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATtg                          <  1:1595639/100‑1 (MQ=255)
                                                                      gctgTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATtg                          <  1:2497213/100‑1 (MQ=255)
                                                                         gTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGa                       >  2:631345/1‑100 (MQ=255)
                                                                            cacTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATg                    >  1:1200169/1‑100 (MQ=255)
                                                                              cTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCg                  <  1:578684/100‑1 (MQ=255)
                                                                              cTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCg                  >  2:2767300/1‑100 (MQ=255)
                                                                               ttAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCgg                 <  1:2742740/100‑1 (MQ=255)
                                                                               ttAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCgg                 >  1:1391124/1‑100 (MQ=255)
                                                                               ttAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCgg                 >  1:1183154/1‑100 (MQ=255)
                                                                                tAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGc                <  2:2355702/100‑1 (MQ=255)
                                                                                tAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGc                >  2:612642/1‑100 (MQ=255)
                                                                                tAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGc                >  1:3148537/1‑100 (MQ=255)
                                                                                tAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGc                >  2:3572231/1‑100 (MQ=255)
                                                                                tAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGc                >  2:2885490/1‑100 (MQ=255)
                                                                                tAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGc                <  1:194561/100‑1 (MQ=255)
                                                                                 aaGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGCt               >  1:3704666/1‑100 (MQ=255)
                                                                                  aGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGc                <  2:2808963/98‑1 (MQ=255)
                                                                                   gAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGCTaa             >  2:3606543/1‑100 (MQ=255)
                                                                                   gAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGCTaa             >  1:333948/1‑100 (MQ=255)
                                                                                     aaCAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGGAGGGTGATATTCCCAAACTGGCCGTAGATGCGATTGTGACTGCGGCTAAt            >  1:3768432/1‑99 (MQ=255)
                                                                                      aCAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGCTAATcc          >  1:61293/1‑100 (MQ=255)
                                                                                      aCAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGCTAATcc          >  2:1699477/1‑100 (MQ=255)
                                                                                           aagGTGTAAGGAGGCGTTATGAAAACGCGTATACATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGCTAATCCGTCa      >  2:226129/1‑99 (MQ=255)
                                                                                             ggTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGCTAATCCGTCAtt    >  1:2768286/1‑99 (MQ=255)
                                                                                              gtgtAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGCTAATCCGTCATTaa  <  1:307947/100‑1 (MQ=255)
                                                                                               |                                                                                                  
ATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGCTAATCCGTCATTAA  >  NC_000913/1105711‑1105904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: