BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,127,049	A→G	60.1%	T82A (ACG→GCG)	yceM →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,127,049	0	A	G	60.1%	95.2 / 133.3	130	T82A (ACG→GCG)	yceM	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (26/26); new base G (35/43); total (61/69)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

TGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAAC  >  NC_000913/1126950‑1127148

 ggCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTAc                                                                                                    >  1:704158/1‑100 (MQ=255)

 ggCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTAc                                                                                                    >  2:3705314/1‑100 (MQ=255)

   cgcATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGt                                                                                                  >  1:533237/1‑100 (MQ=255)

   cgcATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGt                                                                                                  >  1:880933/1‑100 (MQ=255)

    gcATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGtt                                                                                                 >  1:3470741/1‑100 (MQ=255)

    gcATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGtt                                                                                                 <  1:336506/100‑1 (MQ=255)

      aTTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTAc                                                                                               <  2:3890567/100‑1 (MQ=255)

       ttCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACt                                                                                              <  1:806345/100‑1 (MQ=255)

            tATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCCTTCCAGCACCGCCAGCCCCTTTGACGTGGTCAGCGCGTTACCCAATg                                                                                         >  2:3258766/1‑100 (MQ=255)

              tGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCg                                                                                       >  2:2068117/1‑100 (MQ=255)

              tGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCg                                                                                       >  1:520030/1‑100 (MQ=255)

              tGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCg                                                                                       >  1:3123694/1‑100 (MQ=255)

               gCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTCTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCCGTGCGTTACTCAATGCgg                                                                                      >  1:1851715/1‑100 (MQ=255)

                ccGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCggg                                                                                     <  2:1796676/100‑1 (MQ=255)

                ccGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCggg                                                                                     <  1:2093804/100‑1 (MQ=255)

                 cGATTCGTTATACAGCCTTGACGCCAGTTGTGATGCTGTTTTTGTTCATTCCTTAACCGCCAGCCACTTTTTCGTGGTCAGTACGTTAGTCAATGc                                                                                        <  2:1340739/96‑1 (MQ=255)

                  gATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCggggg                                                                                   >  1:3166721/1‑100 (MQ=255)

                  gATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCggggg                                                                                   >  2:2356381/1‑100 (MQ=255)

                   aTTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGt                                                                                  <  2:3153458/100‑1 (MQ=255)

                     tCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTAc                                                                                <  2:643740/100‑1 (MQ=255)

                      cGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGTGGGGGTACa                                                                               <  1:3949819/100‑1 (MQ=255)

                      cGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACa                                                                               >  2:3694303/1‑100 (MQ=255)

                      cGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACa                                                                               <  2:1995251/100‑1 (MQ=255)

                      cGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTGGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACa                                                                               <  1:2407561/100‑1 (MQ=255)

                       gTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACAt                                                                              <  2:2466319/100‑1 (MQ=255)

                       gTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACAt                                                                              <  2:307146/100‑1 (MQ=255)

                         tATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATg                                                                             <  1:4089885/99‑1 (MQ=255)

                         tATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGt                                                                            >  2:471337/1‑100 (MQ=255)

                          cttccgatctGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTc                                                                           <  1:1557766/91‑1 (MQ=255)

                          aTCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTc                                                                           >  1:755656/1‑100 (MQ=255)

                          aTCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTc                                                                           >  1:2072615/1‑100 (MQ=255)

                           tCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCt                                                                          >  2:1129073/1‑100 (MQ=255)

                           tCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCt                                                                          >  1:1490960/1‑100 (MQ=255)

                           tCCAGCCTTCCCGCCAGTTGCGATGCGGCTTTTGTGCATTCCAGCACCGCCAGCCCCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTc                                                                           >  2:29930/1‑99 (MQ=255)

                             cAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCtgt                                                                        <  2:1984294/100‑1 (MQ=255)

                              aGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCtgtg                                                                       >  2:2410441/1‑100 (MQ=255)

                                ccTTGCCGCCAGTTGCGATGCGGTTTTTGAGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTc                                                                     <  2:2478106/100‑1 (MQ=255)

                                   tGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGa                                                                   <  1:3411834/99‑1 (MQ=255)

                                      cgccAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATaaa                                                               >  1:2370705/1‑100 (MQ=255)

                                       gccAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAAc                                                              >  1:3506997/1‑100 (MQ=255)

                                        ccAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAAcc                                                             <  1:1148385/100‑1 (MQ=255)

                                          aGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGc                                                           >  2:83447/1‑100 (MQ=255)

                                              gCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGCCGTGGTCAGTACGTTACTCAATGCGGGGGTGCATGTCTGTGTCGATAAACCGCTGGc                                                       >  1:3992196/1‑100 (MQ=255)

                                              gCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGc                                                       <  1:1413428/100‑1 (MQ=255)

                                              gCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGc                                                       <  1:3881824/100‑1 (MQ=255)

                                                 aTGCGGTTTTTGTGGATGCCAGCACCGCCAGCCACTTTGACGTGGTGAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAg                                                     <  2:2731423/99‑1 (MQ=255)

                                                 aTGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGa                                                    <  2:2746426/100‑1 (MQ=255)

                                                 aTGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGa                                                    <  2:2747997/100‑1 (MQ=255)

                                                  tGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGaa                                                   >  1:518767/1‑100 (MQ=255)

                                                    cGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGaaaa                                                 <  1:3018419/100‑1 (MQ=255)

                                                    cGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGaaaa                                                 <  1:651395/100‑1 (MQ=255)

                                                       tttttGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCt                                              >  1:137705/1‑100 (MQ=255)

                                                        ttttGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTg                                             >  1:1342443/1‑100 (MQ=255)

                                                         tttGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTgc                                            <  1:2356394/100‑1 (MQ=255)

                                                          ttGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTgcg                                           <  2:755650/100‑1 (MQ=255)

                                                          ttGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTgcg                                           <  2:2072602/100‑1 (MQ=255)

                                                            gtgCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGcgcg                                         <  1:2114834/100‑1 (MQ=255)

                                                             tgCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGAT‑AACCGCTGGCAGAAAAGCTGCGCGAt                                       <  1:177610/100‑1 (MQ=255)

                                                             tgCATTCCAGCACCGCCAGCCACTTTCACGTGGTCAGTACGTTACTCAATGCGGGGGTACCTGCCTGTGTCGATAAACCGCTGGCGGAAAATCTGCGCGa                                        >  2:3684239/1‑100 (MQ=255)

                                                              gCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGAt                                       <  2:2347266/100‑1 (MQ=255)

                                                               cATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATg                                      >  1:2150877/1‑100 (MQ=255)

                                                                aTTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGc                                     <  1:786784/100‑1 (MQ=255)

                                                                  tCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTCCTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTg                                   >  2:822505/1‑100 (MQ=255)

                                                                    cAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGaa                                 >  1:3527351/1‑100 (MQ=255)

                                                                     aGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGCTACACCGCTAGCAGAAAATCTGCGCGATCCTGAAc                                >  1:1262312/1‑100 (MQ=255)

                                                                      gCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACg                               <  2:2982583/100‑1 (MQ=255)

                                                                      gCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACg                               >  1:4010906/1‑100 (MQ=255)

                                                                         ccGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCt                            >  2:2064286/1‑100 (MQ=255)

                                                                          cGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGTGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCt                            <  1:624082/99‑1 (MQ=255)

                                                                          cGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCtg                           <  1:207584/100‑1 (MQ=255)

                                                                          cGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCtg                           <  2:1570055/100‑1 (MQ=255)

                                                                           gccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCtgg                          <  2:1575198/100‑1 (MQ=255)

                                                                           gccagccaCTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCtgg                          >  2:3754863/1‑100 (MQ=255)

                                                                           gccagccaCTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCtgg                          >  2:3352450/1‑100 (MQ=255)

                                                                            ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCtggt                         >  2:3005579/1‑100 (MQ=255)

                                                                              agccaCTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCtggtgg                       <  1:4111440/100‑1 (MQ=255)

                                                                              agccaCTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCtggtgg                       >  1:2627094/1‑100 (MQ=255)

                                                                               gccaCTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGa                      >  1:3853086/1‑100 (MQ=255)

                                                                                 caCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAAc                    <  2:114623/100‑1 (MQ=255)

                                                                                 caCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAAc                    <  1:3198654/100‑1 (MQ=255)

                                                                                 caCTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAAc                    >  1:1262980/1‑100 (MQ=255)

                                                                                   cTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTg                  <  1:894506/100‑1 (MQ=255)

                                                                                   cTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTg                  <  2:2307233/100‑1 (MQ=255)

                                                                                   cTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTg                  <  1:991881/100‑1 (MQ=255)

                                                                                   cTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTg                  <  2:1363361/100‑1 (MQ=255)

                                                                                   cTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTg                  >  1:3263949/1‑100 (MQ=255)

                                                                                     ttGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTggc                <  2:1134310/100‑1 (MQ=255)

                                                                                       gACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTggcgg              >  2:3488627/1‑100 (MQ=255)

                                                                                       gACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTggcgg              >  1:343239/1‑100 (MQ=255)

                                                                                        acatGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAACATTTGCGCGATGCTGAACGGCTGGTGGAACTggcggc             <  2:1650179/97‑1 (MQ=255)

                                                                                        aCGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTggcggc             >  2:1382222/1‑100 (MQ=255)

                                                                                           tGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGcgcg          <  1:3983132/100‑1 (MQ=255)

                                                                                           tGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGcgcg          <  1:1162818/100‑1 (MQ=255)

                                                                                            ggTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCgg         <  1:2842345/100‑1 (MQ=255)

                                                                                            ggTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCgg         >  1:2901712/1‑100 (MQ=255)

                                                                                            ggTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCgg         <  2:3336219/100‑1 (MQ=255)

                                                                                            ggTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCgg         >  1:4132809/1‑100 (MQ=255)

                                                                                            ggTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCgg         >  2:3834399/1‑100 (MQ=255)

                                                                                             gTCATTGCGTTACTCAATGCGGCGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGcgcg          <  2:395599/98‑1 (MQ=255)

                                                                                                   aCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAAc  <  1:3237488/100‑1 (MQ=255)

TGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAAC  >  NC_000913/1126950‑1127148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`