Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,197,340 T→C 23.3% T65A (ACT→GCT)  ymfD ← e14 prophage; putative SAM‑dependent methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,197,3400TC23.3% 225.2 / 69.3 128T65A (ACT→GCT) ymfDe14 prophage; putative SAM‑dependent methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (48/50);  new base C (18/12);  total (66/62)
Fisher's exact test for biased strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

Only 100 of 128 total aligned reads displayed.

TGCATTTTTATAATATCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACT  >  NC_000913/1197242‑1197438
                                                                                                  |                                                                                                  
tGCATTTTTATAATATCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTa                                                                                                   <  1:566972/100‑1 (MQ=255)
tGCATTTTTATAATATCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGATCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTa                                                                                                   >  2:2072583/1‑100 (MQ=255)
 gCATTTTTATAATATCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTaa                                                                                                  <  1:3895008/100‑1 (MQ=255)
 gCATTTTTATAATATCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTaa                                                                                                  <  1:3894228/100‑1 (MQ=255)
 gCATTTTTATAATATCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTaa                                                                                                  <  1:1808456/100‑1 (MQ=255)
  cATTTTTATAATATCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAAc                                                                                                 >  1:4117753/1‑100 (MQ=255)
   aTTTTTATAATATCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAAc                                                                                                 <  2:1186116/99‑1 (MQ=255)
      tttATAATATCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTtca                                                                                             <  1:2672500/100‑1 (MQ=255)
      tttATAATATCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTtca                                                                                             <  2:161582/100‑1 (MQ=255)
       ttATAATATCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTtcat                                                                                            >  2:3370180/1‑100 (MQ=255)
         ataataTCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTtcatca                                                                                          <  2:1886648/100‑1 (MQ=255)
         ataataTCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTtcatca                                                                                          <  2:2959451/100‑1 (MQ=255)
            atatCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAAt                                                                                       >  2:2282270/1‑100 (MQ=255)
            atatCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAAt                                                                                       <  2:1440464/100‑1 (MQ=255)
            atatCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAAt                                                                                       >  1:235685/1‑100 (MQ=255)
                cGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTAc                                                                                   >  2:711118/1‑100 (MQ=255)
                cGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTAGAGTCTAGAAAAGTAACTTCATCAAATTTAc                                                                                   >  1:3612566/1‑100 (MQ=255)
                 gTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACt                                                                                  <  1:3027702/100‑1 (MQ=255)
                 gTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTGGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACt                                                                                  <  1:1302428/100‑1 (MQ=255)
                  tgtgACATATTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGTCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTAc                                                                                   <  2:1849797/95‑1 (MQ=255)
                  tGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTg                                                                                 <  1:1902463/100‑1 (MQ=255)
                  tGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTg                                                                                 >  1:66814/1‑100 (MQ=255)
                      aCATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGAtt                                                                              <  2:666816/99‑1 (MQ=255)
                      aCATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTa                                                                             <  1:1949544/100‑1 (MQ=255)
                       cATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTaa                                                                            <  1:2538529/100‑1 (MQ=255)
                        aTAGTGAATAATTTTAGTTTTTATTCCTCGATGAATTTGCGCTCTCTCAAGTTTCCTTTTCGAGTCTAGGAAAGTAACTTCATCAAATTTCCTGATTAAt                                                                           <  2:731869/100‑1 (MQ=255)
                          aGTCCATAATTTTAGTTTTAATTCCTCTAATAATTTGCTATCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATtc                                                                         <  1:1796688/100‑1 (MQ=255)
                          aGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATtc                                                                         >  2:1796675/1‑100 (MQ=255)
                          aGTCAATAATTTTAGTTTTAATTCCTCAGATAATTTGCCCTCTTTCAAGTTACCTTTTCGAGACTTGACACTTAACTTCATGAAATTTGCTGCTTAAt                                                                           >  2:2051576/1‑98 (MQ=255)
                           gTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAATTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATtca                                                                        >  2:70462/1‑100 (MQ=255)
                           gTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAAGTTAATCAAATTTACTGATTAATtca                                                                        >  2:1091952/1‑100 (MQ=255)
                           gTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATtca                                                                        <  1:3644986/100‑1 (MQ=255)
                            tCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATtcat                                                                       <  1:1420027/100‑1 (MQ=255)
                             cAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATtcat                                                                       >  2:280257/1‑99 (MQ=255)
                             cAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATtcatc                                                                      >  1:3415750/1‑100 (MQ=255)
                             cAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATtcat                                                                       <  1:4116112/99‑1 (MQ=255)
                             cAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTATAGAAATGTAACTTCATCAAATTTACTGATTAATtca                                                                        >  2:3880008/1‑98 (MQ=255)
                              aataatTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATtcatca                                                                     >  1:1473745/1‑100 (MQ=255)
                               ataatTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAATTTGCCTTTTCGAGTCTATAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAg                                                                    >  2:876653/1‑100 (MQ=255)
                               ataatTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATtcatc                                                                      <  2:1906894/98‑1 (MQ=255)
                               ataatTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAg                                                                    <  2:3260900/100‑1 (MQ=255)
                                     ttAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATc                                                              <  2:2693686/100‑1 (MQ=255)
                                      tAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCt                                                             >  1:1436410/1‑100 (MQ=255)
                                         ttttAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAg                                                          <  1:2467441/100‑1 (MQ=255)
                                                tCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCg                                                   <  1:961450/100‑1 (MQ=255)
                                                  ctctAATAATTTGCTCTCTTTCAAGTTGCCTTTTCTAGTATAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATAAGAATATCTAAGATTTCTGCa                                                 >  2:1839072/1‑100 (MQ=255)
                                                  ctctAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTTGAAAACTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTcc                                                    <  1:1344971/97‑1 (MQ=255)
                                                  ctctAATAATTGGCTCTCTATCAAGTTGCCTTTTCGAAGATAGAAAAGTAACTTCATTAAATTTACTTGGTAAGTTATGGGAATATCTAAGTTTTTCgta                                                 >  2:2547051/1‑98 (MQ=255)
                                                    ctAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGc                                               >  2:3777543/1‑100 (MQ=255)
                                                    ctAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAg                                                <  1:3895487/99‑1 (MQ=255)
                                                     taataaTTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGcc                                              <  1:494161/100‑1 (MQ=255)
                                                     taataaTTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGcc                                              >  1:2415114/1‑100 (MQ=255)
                                                      aataatTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCa                                             >  2:2827876/1‑100 (MQ=255)
                                                        taatTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCaaa                                           >  2:3338369/1‑100 (MQ=255)
                                                         aatTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCaaaa                                          >  1:2713550/1‑100 (MQ=255)
                                                         aatTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCaaaa                                          <  1:785288/100‑1 (MQ=255)
                                                          atTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAAt                                         <  1:2846390/100‑1 (MQ=255)
                                                            ttGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCa                                       >  1:527499/1‑100 (MQ=255)
                                                            ttGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCa                                       <  2:2869681/100‑1 (MQ=255)
                                                             tGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCaa                                      >  2:1845592/1‑100 (MQ=255)
                                                             tGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCaa                                      >  1:3131906/1‑100 (MQ=255)
                                                               ctctctTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAgg                                    >  1:2871949/1‑100 (MQ=255)
                                                               ctctctTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAgg                                    >  1:3385733/1‑100 (MQ=255)
                                                                tctctTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAggg                                   <  1:1464110/100‑1 (MQ=255)
                                                                tctctTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAggg                                   <  1:3880033/100‑1 (MQ=255)
                                                                   ctTTCAAGTTGCGTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGt                                >  1:2714083/1‑100 (MQ=255)
                                                                       cAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGcc                            >  1:3167885/1‑100 (MQ=255)
                                                                          gTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCAtt                         <  1:1648779/100‑1 (MQ=255)
                                                                          gTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCAtt                         >  1:1475539/1‑100 (MQ=255)
                                                                            tGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCAtttt                       >  2:3134168/1‑100 (MQ=255)
                                                                             gCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCAttttt                      >  2:422014/1‑100 (MQ=255)
                                                                             gCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCAttttt                      >  2:161904/1‑100 (MQ=255)
                                                                              ccTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTtc                     <  2:1677410/100‑1 (MQ=255)
                                                                              ccTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTtc                     >  1:586316/1‑100 (MQ=255)
                                                                              ccTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTtc                     <  1:615602/100‑1 (MQ=255)
                                                                               cTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTtct                    >  1:1328074/1‑100 (MQ=255)
                                                                               cTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACAGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTtct                    >  2:2214463/1‑100 (MQ=255)
                                                                                ttttCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTtctc                   <  1:3090248/100‑1 (MQ=255)
                                                                                 tttCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCa                  <  2:320177/100‑1 (MQ=255)
                                                                                  ttCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCaa                 <  2:3612305/100‑1 (MQ=255)
                                                                                  ttCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCaa                 <  1:3049817/100‑1 (MQ=255)
                                                                                    cGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATg               >  2:2915339/1‑100 (MQ=255)
                                                                                     gAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGc              <  2:1326970/100‑1 (MQ=255)
                                                                                     gAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATAACTAAGTTTTCCGCAGCCAAAATCAAGGCCGAGGCCATTTTTCTCAATGc              >  2:1310665/1‑100 (MQ=255)
                                                                                       gTCTAGCAAAGCAACTTCATCAAATTTACTGATTAATTAATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCtt            >  2:843252/1‑100 (MQ=255)
                                                                                       gTCTAGAAAAGTAACTTCATCAAATGTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCtt            <  2:3974271/100‑1 (MQ=255)
                                                                                        tCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTc           >  1:3938517/1‑100 (MQ=255)
                                                                                        tCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTc           <  1:1656258/100‑1 (MQ=255)
                                                                                         cTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCt          >  1:2871808/1‑100 (MQ=255)
                                                                                         cTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCt          >  2:1057753/1‑100 (MQ=255)
                                                                                          tAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTa         >  2:3794331/1‑100 (MQ=255)
                                                                                          tAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAAAGCTTCTa         <  2:2162977/100‑1 (MQ=255)
                                                                                            gAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAat       >  1:1672980/1‑100 (MQ=255)
                                                                                             aaaaGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAat       <  2:3941529/99‑1 (MQ=255)
                                                                                             aaaaGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAata      <  1:2950380/100‑1 (MQ=255)
                                                                                               aaGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAAtata    <  2:2533512/100‑1 (MQ=255)
                                                                                                aGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATAc   >  1:3528134/1‑100 (MQ=255)
                                                                                                 gTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTTCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACt  >  2:708925/1‑100 (MQ=255)
                                                                                                 gTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTTTCAATGCTTCTAATATACt  >  2:2130296/1‑100 (MQ=255)
                                                                                                 gTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTGTTTTCAATGTTTCTAATATAc   >  2:2782079/1‑99 (MQ=255)
                                                                                                  |                                                                                                  
TGCATTTTTATAATATCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACT  >  NC_000913/1197242‑1197438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: