BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,197,739	A→G	100%	S167P (TCC→CCC)	ymfE ←	e14 prophage; putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,197,739	0	A	G	100.0%	390.8 / ‑6.7	116	S167P (TCC→CCC)	ymfE	e14 prophage; putative inner membrane protein
Reads supporting (aligned to +/- strand): ref base A (1/0); major base G (55/58); minor base T (1/1); total (57/59)

Only 100 of 120 total aligned reads displayed.

CTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTA  >  NC_000913/1197643‑1197834

ctGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAAt                                                                                              >  1:2473205/1‑100 (MQ=255)

  nagaATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTa                                                                                            >  1:3411131/2‑100 (MQ=255)

  gagaATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTa                                                                                            <  2:2968007/100‑1 (MQ=255)

    gaATGATAAAATTACTAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAAt                                                                                          <  1:4075615/100‑1 (MQ=255)

    gaATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAAt                                                                                          >  1:1722475/1‑100 (MQ=255)

    gaATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAAt                                                                                          <  1:2625072/100‑1 (MQ=255)

      aTGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATTCAATTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGc                                                                                        >  2:2903118/1‑100 (MQ=255)

      aTGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGc                                                                                        <  1:2197402/100‑1 (MQ=255)

      aTGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTCGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGc                                                                                        <  1:280260/100‑1 (MQ=255)

      aTGAAAAAAATACAAAGAAGAATAAAGACTGCAAAGAACCTGCAACATGAAAAATATCCATTTGTTTGTAAAAAAAGATTATTAGGAAGGGAATTAATGc                                                                                        <  2:311302/100‑1 (MQ=255)

      aTGAAAAAAATACAAAAAAAAATAAAGACAGAAAAGAACCTGTAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGc                                                                                        <  2:2502385/100‑1 (MQ=255)

       tGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCa                                                                                       >  1:549789/1‑100 (MQ=255)

       tGATAAAATGAGAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCa                                                                                       <  2:2625989/100‑1 (MQ=255)

         aTAAAATTACAAAAAAGAATAAAGACAGAAAAGAACCTTAAACATGAAAAATATAAAACAAAAAAAAAAAAAAGATTATTAGGAAGGGAATTAATGc                                                                                        <  2:2545586/97‑1 (MQ=255)

         aTAAAATTACAAAAAAGAATAAAAACAGCAAAGAACCTTCAACATGAAAAATATCCAAATAAAAAAAAAAAAAGATTATTAGGAAGGGAATTAATGCAAt                                                                                     <  2:519373/100‑1 (MQ=255)

          tAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAAtt                                                                                    >  2:1165008/1‑100 (MQ=255)

          tAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAAtt                                                                                    <  2:547838/100‑1 (MQ=255)

          tAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAAtt                                                                                    >  1:1649481/1‑100 (MQ=255)

          tAAAATTACAAAAAAGAATAAAGACAGCAAAAAACCTTCAACATAAAAAATATCCATTAGTATGCAAAAAAAGATTATTAAAAAGGGAATTAAtaaaat                                                                                     >  2:2035145/1‑94 (MQ=255)

            aaaTTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATACATTAGTTTGCAAAAAAAGATTATTAGGAAGGTAATTAATGCAATTAt                                                                                  >  2:2750604/1‑100 (MQ=255)

             aaTTACAAAAAAAAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATATAAAAAAAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATc                                                                                 <  2:2635444/100‑1 (MQ=255)

              aTTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCg                                                                                >  2:3480118/1‑100 (MQ=255)

                atctaaaaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGaa                                                                              <  2:121889/96‑1 (MQ=255)

                  cAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAGAAGATTATTAGGAAGGGAATTAATGCAATTATCGaaaa                                                                            >  2:2245185/1‑100 (MQ=255)

                  cAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGaaaa                                                                            >  2:117461/1‑100 (MQ=255)

                   aaaaaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAAt                                                                           <  1:556722/100‑1 (MQ=255)

                   aaaaaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAAt                                                                           <  2:1979296/100‑1 (MQ=255)

                   aaaaaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAAt                                                                           >  2:2237879/1‑100 (MQ=255)

                    aaaaaGAATAAAGACAGCAAAGAACGTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATGAGGAAGGGAATTAATGCAATTAGCGAAAAtt                                                                          >  2:2464184/1‑100 (MQ=255)

                    aaaaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGaaagat                                                                          >  1:121890/1‑97 (MQ=255)

                    aaaaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAAtt                                                                          >  2:1839212/1‑100 (MQ=255)

                    aaaaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAAtt                                                                          >  1:2630911/1‑100 (MQ=255)

                    aaaaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTAACGAAAAtt                                                                          >  1:168517/1‑100 (MQ=255)

                    aaaaaGAATAAAGACAGCAAAGAACCTTCAAAATGAAAAATATCCATTAGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAAtt                                                                          >  2:1371453/1‑100 (MQ=255)

                     aaaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTc                                                                         >  1:2030963/1‑100 (MQ=255)

                      aaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCa                                                                        >  2:290045/1‑100 (MQ=255)

                        aGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGACGGGAATTAATGCAATTATCGAAAATTCaaa                                                                      <  2:2714067/100‑1 (MQ=255)

                        aGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCAATATAAAAAAAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaa                                                                      <  2:1101212/100‑1 (MQ=255)

                         gAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaca                                                                     <  1:2798969/100‑3 (MQ=255)

                         gAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaaa                                                                     >  1:2172739/1‑100 (MQ=255)

                             aaaGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAata                                                                 >  1:1076567/1‑100 (MQ=255)

                               aGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATc                                                               >  2:1396864/1‑100 (MQ=255)

                                gACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATcc                                                              >  1:1155714/1‑100 (MQ=255)

                                  cAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaa                                                            >  2:92221/1‑100 (MQ=255)

                                  cAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaa                                                            >  2:808521/1‑100 (MQ=255)

                                   aGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaa                                                           <  1:573555/100‑1 (MQ=255)

                                   aGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaa                                                           >  1:2740244/1‑100 (MQ=255)

                                    gCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaa                                                          >  1:2183421/1‑100 (MQ=255)

                                     cAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaaa                                                         >  1:2348778/1‑100 (MQ=255)

                                      aaaGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAAt                                                        <  1:3326450/100‑1 (MQ=255)

                                       aaGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGTAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATa                                                       <  2:1694198/100‑1 (MQ=255)

                                       aaGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATa                                                       >  1:1184194/1‑100 (MQ=255)

                                        aGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAg                                                      <  1:1834831/100‑1 (MQ=255)

                                        aGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAg                                                      <  1:195495/100‑1 (MQ=255)

                                          aaCCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGta                                                    <  1:2118148/100‑1 (MQ=255)

                                            ccTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTTAAAAAATATCCAAAAATAGtata                                                  >  2:3868191/1‑100 (MQ=255)

                                            ccTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGtata                                                  >  2:862702/1‑100 (MQ=255)

                                             cTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGtata                                                  <  2:2339581/99‑1 (MQ=255)

                                             cTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATAc                                                 >  1:1815447/1‑100 (MQ=255)

                                                 aaCATGAAAAGTATACATTTGTTTAAAAAAAAAGATTATTAGTAAGGGAATTAATGCAATTATCAAAAAAAAAAAAAATATCCAAAAATAGTATACTTTa                                             <  2:2725566/100‑1 (MQ=255)

                                                 aaCAAGAAAAATATCCATTTGTTTGCAAAAAAAGATAATTAGGAAGGGAATTAATGCAATTAAAAAAAAAAAAAAAAATATCCAAAAATAGTATACTTTa                                             <  2:3257804/100‑1 (MQ=255)

                                                  aCATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTAt                                            <  1:1718642/100‑1 (MQ=255)

                                                  aCATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTAt                                            >  1:1658896/1‑100 (MQ=255)

                                                    aTGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTc                                          >  1:2015970/1‑100 (MQ=255)

                                                     tGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCGAAAAATATCCAAAAATACTATACTTTATTcc                                         <  1:2782095/100‑1 (MQ=255)

                                                     tGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTcc                                         <  1:2593263/100‑1 (MQ=255)

                                                     tGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTcc                                         <  1:26727/100‑1 (MQ=255)

                                                     tGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTcc                                         <  1:3349482/100‑1 (MQ=255)

                                                     tGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTcc                                         <  1:708931/100‑1 (MQ=255)

                                                     tGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTcc                                         >  1:1644536/1‑100 (MQ=255)

                                                      gAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCa                                        >  1:1447409/1‑100 (MQ=255)

                                                      gAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCa                                        >  1:1031961/1‑100 (MQ=255)

                                                        aaaaTATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCaga                                      >  2:4014900/1‑100 (MQ=255)

                                                        aaaaTATCCATTTGATTGCAAAAAAAGAATATTAAGAAGGGAATTAATGCAATTATCGAAAATTAAAAAAATATCCAAAAATAGTATACTTTATTCCaga                                      <  2:626464/100‑1 (MQ=255)

                                                         aaaTATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCagaa                                     <  1:549747/100‑1 (MQ=255)

                                                           atatCCATTTGTTTGAAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATAGAAAAAAAAAAAAATATCCAAAAATAGTATACTTTATTCCagaaga                                   <  2:65812/100‑1 (MQ=255)

                                                           atatCCATTTGTTAGCAAAAAAAGATTATTAAGAAGAGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCagaag                                    <  2:927018/99‑1 (MQ=255)

                                                             atCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGt                                 >  1:1332258/1‑100 (MQ=255)

                                                             atCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGt                                 <  1:2964178/100‑1 (MQ=255)

                                                               ccATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTc                               >  1:2281006/1‑100 (MQ=255)

                                                               ccATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTc                               <  1:808527/100‑1 (MQ=255)

                                                               ccATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAAATCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTc                               <  1:3366567/100‑1 (MQ=255)

                                                                 aTTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCaa                             <  1:3388158/100‑1 (MQ=255)

                                                                  tttgtttgCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAat                            <  1:1988043/100‑1 (MQ=255)

                                                                  tttgtttgCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAAAAGAGTTCaa                             >  2:1845841/1‑99 (MQ=255)

                                                                    tgtttgCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAatat                          >  1:2093971/1‑100 (MQ=255)

                                                                    tgtttgCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAatat                          <  1:576164/100‑1 (MQ=255)

                                                                     gtttgCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCagaagatcggaagagc                         >  1:2283730/1‑90 (MQ=255)

                                                                      tttgCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATaa                        >  2:1677916/1‑100 (MQ=255)

                                                                         gCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGt                     <  1:3861176/100‑1 (MQ=255)

                                                                         gCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGt                     <  2:2325843/100‑1 (MQ=255)

                                                                          cAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGtt                    >  2:1133688/1‑100 (MQ=255)

                                                                           aaaaaaaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGttt                   >  2:1451560/1‑100 (MQ=255)

                                                                            aaaaaaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTg                  <  2:2183408/100‑1 (MQ=255)

                                                                            aaaaaaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTg                  <  1:2130309/100‑1 (MQ=255)

                                                                                    ttattaGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAAt          <  1:3530994/100‑1 (MQ=255)

                                                                                        taGGAAGGGAATTAATGCAATTATCGAAAATTCAAAACATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAAttttt      >  2:1859106/1‑100 (MQ=255)

                                                                                          ggaagggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTGGTCTTCAATTTTTCt    >  2:3051895/1‑100 (MQ=255)

                                                                                           gaagggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCtt   <  1:2419713/100‑1 (MQ=255)

                                                                                            aagggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGGAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTa  >  1:2898615/1‑100 (MQ=255)

CTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTA  >  NC_000913/1197643‑1197834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`