Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,207,981 G→A 25.2% A161T (GCC→ACC)  stfP → e14 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,207,9810GA25.2% 185.9 / 58.7 107A161T (GCC→ACC) stfPe14 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (39/40);  new base A (15/12);  total (54/53)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

GAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGTTTTTATTACGGTGC  >  NC_000913/1207882‑1208079
                                                                                                   |                                                                                                  
gAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATg                                                                                                    >  2:1195995/1‑100 (MQ=255)
gAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATg                                                                                                    >  1:1974564/1‑100 (MQ=255)
gAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATg                                                                                                    >  2:371644/1‑100 (MQ=255)
  gCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGcc                                                                                                  >  2:2323330/1‑100 (MQ=255)
  gCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGcc                                                                                                  >  2:2808327/1‑100 (MQ=255)
  gCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGcc                                                                                                  <  2:3121078/100‑1 (MQ=255)
  gCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGcc                                                                                                  >  2:2309345/1‑100 (MQ=255)
  gCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATAcc                                                                                                  <  1:150286/100‑1 (MQ=255)
    aaTCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCtg                                                                                                <  2:3259075/100‑1 (MQ=255)
     aTCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCtgt                                                                                               <  1:821594/100‑1 (MQ=255)
      tCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCtgtg                                                                                              <  1:1196003/100‑1 (MQ=255)
       cAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTgtgt                                                                                             >  2:2448636/1‑100 (MQ=255)
          tAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGc                                                                                          >  1:3349589/1‑100 (MQ=255)
          tAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGc                                                                                          <  2:2666360/100‑1 (MQ=255)
          tAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGc                                                                                          <  2:2666618/100‑1 (MQ=255)
            aTCATTCAGTGGGGGTGGAATGACGAAAAAGCATCTGTCTTTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCg                                                                                        <  2:2784187/100‑1 (MQ=255)
              cATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCgtt                                                                                      >  1:1043582/1‑100 (MQ=255)
                ttCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCgttgt                                                                                    >  2:1668340/1‑100 (MQ=255)
                  cAGTGGGGGTTGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGttgttg                                                                                  <  1:3929154/100‑1 (MQ=255)
                    gTGGGGGTGGAATGCCGCAAAAGCATCTGCCGCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTGGCCGTTGTTGc                                                                                 <  2:3581157/99‑1 (MQ=255)
                      gggggTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCaaa                                                                              >  1:784746/1‑100 (MQ=255)
                      gggggTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATACCTGTGTTGCCGTTGTTGCaaa                                                                              >  1:3232535/1‑100 (MQ=255)
                       ggggTGGAATGCCGCAAAAGCATCTGCCTCTGGGGTGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATACCTGTGTTGCCGTTGTTGCAAAt                                                                             <  2:513389/100‑1 (MQ=255)
                       ggggTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATACCTGTGTTGCCGTTGTTGCAAAt                                                                             >  1:1149407/1‑100 (MQ=255)
                       ggggTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATACCTGTGTTGCCGTTGTTGCAAAt                                                                             >  2:1072726/1‑100 (MQ=255)
                         ggTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTa                                                                           <  2:2632947/100‑1 (MQ=255)
                         ggTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTa                                                                           >  1:3077456/1‑100 (MQ=255)
                          gTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTAt                                                                          <  1:1051240/100‑1 (MQ=255)
                           taaaaTCCCACAAAAGCATCGTCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATACCTGTGTTGCCGTTGTTGCAAAt                                                                             <  2:2584876/93‑1 (MQ=255)
                           tGGAAGGCCGCAAAAGCATCTGCCTCAGAGGGGGATGGAAAAAAAGTCGAATTAAAGGTGGCGGTTAATAAGACCTGGGTTGCCGTTGTTACAAATTa                                                                           >  2:3711672/1‑98 (MQ=255)
                            ggAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGa                                                                        >  2:3525726/1‑100 (MQ=255)
                            ggAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGa                                                                        <  1:1087709/100‑1 (MQ=255)
                             gAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGAc                                                                       <  2:3634132/100‑1 (MQ=255)
                                 gCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATg                                                                   >  2:451562/1‑100 (MQ=255)
                                 gCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGACTGTGTTGCCGTTGTTGCAAATTATGACAATg                                                                   >  2:1774230/1‑100 (MQ=255)
                                  ccGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGt                                                                  >  1:918020/1‑100 (MQ=255)
                                   cGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACGGTTGTATTCCCGGTTGCGTTTAATAATACCTGTGTTGCCGTTGTTGCAAATTATGACAATGTc                                                                 >  1:2913153/1‑100 (MQ=255)
                                   cGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTc                                                                 <  1:256457/100‑1 (MQ=255)
                                     cAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATACCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAg                                                               >  1:3082955/1‑100 (MQ=255)
                                          gCATCTGCCTCTGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATACCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCAcc                                                         <  1:738260/100‑1 (MQ=255)
                                           cagcTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATACCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCAcc                                                         <  1:2497063/97‑1 (MQ=255)
                                               tGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATc                                                     >  2:336064/1‑100 (MQ=255)
                                               tGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATc                                                     >  2:1916607/1‑100 (MQ=255)
                                                 ccTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATACCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCaa                                                   <  2:2366011/100‑1 (MQ=255)
                                                  ctctGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAAt                                                  >  1:3789706/1‑100 (MQ=255)
                                                    ctGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGc                                                <  2:1283927/100‑1 (MQ=255)
                                                    ctGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATACCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGc                                                <  2:3082936/100‑1 (MQ=255)
                                                      gggggggATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAg                                              >  1:2819191/1‑100 (MQ=255)
                                                        gggggATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTg                                            >  2:1782713/1‑100 (MQ=255)
                                                         ggggATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATACCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTgg                                           <  1:2487556/100‑1 (MQ=255)
                                                         ggggATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATACCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTgg                                           <  1:4027809/100‑1 (MQ=255)
                                                          gggATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGc                                          <  2:1692867/100‑1 (MQ=255)
                                                          gggATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGc                                          <  1:371647/100‑1 (MQ=255)
                                                           ggATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCa                                         >  2:3044690/1‑100 (MQ=255)
                                                           ggATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATCCCTGTCTTGCCGTTGTTGCAAATTACGACAATGTCAGCGCACCTATCAATGCAGTGGc                                          <  2:3477678/99‑1 (MQ=255)
                                                            gATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCaa                                        >  1:492917/1‑100 (MQ=255)
                                                              tGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACg                                      >  1:2956853/1‑100 (MQ=255)
                                                                 aaaTACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACgggg                                   <  1:2371624/100‑1 (MQ=255)
                                                                  aaTACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACggggg                                  >  2:182753/1‑100 (MQ=255)
                                                                  aaTACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAAGGTCAGCGCACCTATCAATGCAGTGGCAACggggg                                  <  2:2643616/100‑1 (MQ=255)
                                                                   aTACAGTTGTATTCCCGGTTGCGTTTAATAATACCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACgggggg                                 <  1:147865/100‑1 (MQ=255)
                                                                    tACAGTTGTATTCCCGGTTGCGTTTAATACTGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTTAGCGCACCTATCAATGCAGTGGCAACGGGGGGa                                <  2:2478644/100‑1 (MQ=255)
                                                                    tACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGa                                >  2:2630947/1‑100 (MQ=255)
                                                                     aCAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGat                               <  2:2413351/100‑1 (MQ=255)
                                                                      ccgTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGata                              <  2:2510117/98‑1 (MQ=255)
                                                                      cAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGata                              >  1:3103061/1‑100 (MQ=255)
                                                                      cAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGata                              >  2:3690872/1‑100 (MQ=255)
                                                                      cAGTTGTATTCCCGGTTGCGTTTAATAATACCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGata                              >  2:472049/1‑100 (MQ=255)
                                                                      cAGTTGTATTCCCGGTTGCGTTTAATAATACCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGata                              <  2:2156356/100‑1 (MQ=255)
                                                                       aGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGatat                             <  1:2754937/100‑1 (MQ=255)
                                                                         ttGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATAc                           <  2:3704131/100‑1 (MQ=255)
                                                                         ttGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATAc                           <  1:21984/100‑1 (MQ=255)
                                                                          tGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACa                          <  1:3525749/100‑1 (MQ=255)
                                                                            tATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAAc                        <  1:3036010/100‑1 (MQ=255)
                                                                              ttCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCa                      <  1:2384663/100‑1 (MQ=255)
                                                                              ttCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCa                      >  2:2013605/1‑100 (MQ=255)
                                                                              ttCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCa                      <  2:3215035/100‑1 (MQ=255)
                                                                                cccGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAACGTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGctaaagcac                        >  2:2807711/1‑90 (MQ=255)
                                                                                cccGGTTGCGTTTAATAATACCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACt                    >  1:2987609/1‑100 (MQ=255)
                                                                                 ccGGTTGCGTTTAATAATACCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACtt                   >  1:3915324/1‑100 (MQ=255)
                                                                                      tGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGttt              <  2:606614/100‑1 (MQ=255)
                                                                                       gcctttAATAATGCCTGTGTTGCCATTGTTCCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGtttt             <  1:1670430/97‑1 (MQ=255)
                                                                                       gCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGtttt             >  2:191567/1‑100 (MQ=255)
                                                                                       gCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGtttt             <  1:198177/100‑1 (MQ=255)
                                                                                       gCGTTTAATAATGCCTCTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGtttt             <  2:2415336/100‑1 (MQ=255)
                                                                                        cGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGttttt            >  2:1365651/1‑100 (MQ=255)
                                                                                        cGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGttttt            >  1:2291371/1‑100 (MQ=255)
                                                                                        cGTTTAATAATGCCTGTGTTGCCGTTATTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGttttt            >  1:2507523/1‑100 (MQ=255)
                                                                                        cGTTTAATAATACGTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGttttt            <  2:1579918/100‑1 (MQ=255)
                                                                                         gTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGTTTtta           >  1:588506/1‑100 (MQ=255)
                                                                                         gTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGTTTtta           <  2:4113829/100‑1 (MQ=255)
                                                                                         gTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGAACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGTTTtta           <  2:3809155/100‑1 (MQ=255)
                                                                                         gTTTAATAATGCCTGTGTTGCCGTTGCTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGTGGGATATACAACCACTTCGttttt            >  2:1558188/1‑99 (MQ=255)
                                                                                         gTTTAATAATACCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGTTTtta           >  1:1715454/1‑100 (MQ=255)
                                                                                          tttAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGTTTttat          <  1:1877762/100‑1 (MQ=255)
                                                                                          tttAATAATACCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGTTTttat          >  2:1322367/1‑100 (MQ=255)
                                                                                             aataatGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGTTTTTATTAc       <  1:3143256/100‑1 (MQ=255)
                                                                                             aataatGCCTGCGTTGCCATTGTAGCAAATTATGACAATGTCAGCGCACCCATCAATGCAGCGGCAACTGGGGGATATACACCCACTTCGTTTTTGTTAc       >  2:1064753/1‑100 (MQ=255)
                                                                                             aataatAACTGAGTTGCCGTTGTTGCAAATTATAACAATGTCAACGCACCTATCAATGCAGTGGAAACAGGGGAATATACAACCACTTCGttttttt          >  2:2623942/1‑95 (MQ=255)
                                                                                               naatACCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGTTTTTATTACgg     >  1:3411108/2‑100 (MQ=255)
                                                                                                   |                                                                                                  
GAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGTTTTTATTACGGTGC  >  NC_000913/1207882‑1208079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: