Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,303,332 T→G 100% V145G (GTG→GGG)  oppB → oligopeptide ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,303,3320TG59.0% 105.3 / 157.7 136V145G (GTG→GGG) oppBoligopeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (31/25);  new base G (39/41);  total (70/66)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 139 total aligned reads displayed.

TGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGC  >  NC_000913/1303234‑1303427
                                                                                                  |                                                                                               
tgtTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACAGGGGTTGTTATCCCCAGTTTtgtg                                                                                                >  2:1964307/1‑100 (MQ=255)
 gtTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTgggg                                                                                               <  1:2494929/100‑1 (MQ=255)
 gtTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTgggg                                                                                               <  1:2579827/100‑1 (MQ=255)
 gtTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTgg                                                                                               <  1:3230453/100‑1 (MQ=255)
   tAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGtt                                                                                             >  1:2495459/1‑100 (MQ=255)
        cTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCAcc                                                                                        >  1:2447910/1‑100 (MQ=255)
        cTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCAcc                                                                                        <  1:866061/100‑1 (MQ=255)
          ggCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCat                                                                                      <  2:2503596/100‑1 (MQ=255)
          ggCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCat                                                                                      <  1:3441647/100‑1 (MQ=255)
           gCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCat                                                                                      <  2:3464436/99‑1 (MQ=255)
                aTTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAg                                                                                >  2:3843254/1‑100 (MQ=255)
                aTTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAg                                                                                <  2:1743233/100‑1 (MQ=255)
                 ttGTCGCATTAAAATAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAg                                                                                <  1:1646137/99‑1 (MQ=255)
                 ttGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGt                                                                               >  1:202190/1‑100 (MQ=255)
                 ttGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGt                                                                               <  1:2976967/100‑1 (MQ=255)
                  tGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTc                                                                              >  1:3016107/1‑100 (MQ=255)
                  tGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTc                                                                              <  1:771720/100‑1 (MQ=255)
                   gCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCa                                                                             <  1:2817560/100‑1 (MQ=255)
                   gCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCa                                                                             >  2:810791/1‑100 (MQ=255)
                   gCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCa                                                                             >  1:2278359/1‑100 (MQ=255)
                   gCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGCTGCACCATTATTAGtga                                                                             >  2:4074590/1‑98 (MQ=255)
                     cGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATg                                                                           <  2:3424698/100‑1 (MQ=255)
                      gCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGa                                                                          >  2:3509544/1‑100 (MQ=255)
                      gCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTCTGGGGTTGCACCATTATTAGTCATGa                                                                          >  2:2297307/1‑100 (MQ=255)
                           aaaacaaaacACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATAttt                                                                     >  2:2826140/1‑100 (MQ=255)
                           aaaacaaaacACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATAttt                                                                     >  1:821882/1‑100 (MQ=255)
                           aaaacaaaacACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGATGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATAttt                                                                     >  2:1738932/1‑100 (MQ=255)
                            aaacaaaacaCCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTg                                                                    >  2:2755071/1‑100 (MQ=255)
                            aaacaaaacaCCAAATGGGACTATACCGTGATGGGACTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTg                                                                    >  1:1483752/1‑100 (MQ=255)
                             aacaaaacaCCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGc                                                                   <  2:2044439/100‑1 (MQ=255)
                              acaaaacaCCAAATGGGGCTGTACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTg                                                                    <  2:4136438/98‑1 (MQ=255)
                               caaaacaCCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGa                                                                 <  1:1989782/100‑1 (MQ=255)
                                aaaacaCCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGAt                                                                >  1:608006/1‑100 (MQ=255)
                                 aaacaCCAAATGGGACTATACCGTGATGGGGCTGGCAATGAGCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATc                                                               >  2:2260995/1‑100 (MQ=255)
                                 aaacaCCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATc                                                               <  2:752331/100‑1 (MQ=255)
                                    cacCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCAtt                                                            >  2:1092844/1‑100 (MQ=255)
                                    cacCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCAtt                                                            >  2:1307977/1‑100 (MQ=255)
                                     acCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCAttt                                                           >  1:2939852/1‑100 (MQ=255)
                                       cAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTg                                                         <  1:2121191/100‑1 (MQ=255)
                                       cAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTg                                                         <  2:3973360/100‑1 (MQ=255)
                                       cAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGAGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTg                                                         <  1:442818/100‑1 (MQ=255)
                                       cAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCACCAGTTTTGGGGTTGCAGTATTATTAGTCATGGTATTTGCGATCATTTTg                                                         >  2:803770/1‑100 (MQ=255)
                                          aTGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCAt                                                      <  2:406908/100‑1 (MQ=255)
                                           tGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCAtt                                                     <  1:167835/100‑1 (MQ=255)
                                            gggACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTg                                                    <  2:519801/100‑1 (MQ=255)
                                             ggACTATTCCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTgg                                                   <  2:3670450/100‑1 (MQ=255)
                                                cTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTg                                                >  1:2932125/1‑100 (MQ=255)
                                                  ataCCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCGCCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGcc                                              <  1:1222058/100‑1 (MQ=255)
                                                     ccGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCggg                                           >  2:3550836/1‑100 (MQ=255)
                                                     ccGTGATGGGGATGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGAGATAATTTTGCAtt                                                     >  2:3583351/1‑90 (MQ=255)
                                                       gTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCg                                         >  1:1641556/1‑100 (MQ=255)
                                                           tGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTgg                                     <  1:2282931/100‑1 (MQ=255)
                                                            ggggCTGGCAATGACCGGGGTTGTTATCCCCAGGTTTGGGGTTGCACCATTATTAGTCATGATATTTTCGATGATTTTGCATTGGCTGCCGGGCGGTGGc                                    >  2:2572171/1‑100 (MQ=255)
                                                             gggCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGGCgg                                            >  2:1030669/1‑91 (MQ=255)
                                                             gggCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTgg                                     <  2:138054/98‑1 (MQ=255)
                                                              ggCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGGTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGc                                    <  1:1973591/98‑1 (MQ=255)
                                                              ggCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTg                                  >  2:2981433/1‑100 (MQ=255)
                                                              ggCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTg                                  <  2:1068587/100‑1 (MQ=255)
                                                              ggCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTg                                  >  2:3903454/1‑100 (MQ=255)
                                                               gCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTgg                                 <  1:3685243/100‑1 (MQ=255)
                                                               gCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTgg                                 <  1:2868822/100‑1 (MQ=255)
                                                                cTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGa                                >  2:1550365/1‑100 (MQ=255)
                                                                 tGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGa                                >  1:1546793/1‑99 (MQ=255)
                                                                  ggCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAAt                              >  1:1961157/1‑100 (MQ=255)
                                                                  ggCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAAt                              <  1:359640/100‑1 (MQ=255)
                                                                  ggCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAAt                              <  2:1698569/100‑1 (MQ=255)
                                                                    cAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAAtgg                            <  1:415131/100‑1 (MQ=255)
                                                                    cAATGACCGGGGTTGTTATCCCCAGTTCTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAAtgg                            >  1:3589029/1‑100 (MQ=255)
                                                                     aaTGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAAtggt                           <  1:3683906/100‑1 (MQ=255)
                                                                     aaTGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAAtgg                            <  2:698078/99‑1 (MQ=255)
                                                                       tGACCGGGGTTGCTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAAtggtgg                         >  1:3352546/1‑100 (MQ=255)
                                                                         aCCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTgggg                       <  2:1654856/100‑1 (MQ=255)
                                                                          ccGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGgc                      <  1:1497212/100‑1 (MQ=255)
                                                                          ccGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGAGATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTgggg                       <  2:1389750/99‑1 (MQ=255)
                                                                           cGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGgcg                     <  1:3321976/100‑1 (MQ=255)
                                                                           cGGGGGTGTTATCACCAGTTTTGTGGTTGCACCATTAGTGGTCATGATATGTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGGTGGAAtggtg                          <  2:268797/95‑1 (MQ=255)
                                                                              ggTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCtt                  <  1:3956096/100‑1 (MQ=255)
                                                                              ggTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCt                   <  2:1834744/99‑1 (MQ=255)
                                                                               gttgttATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTa                 <  1:1435267/100‑1 (MQ=255)
                                                                               gttgttATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTa                 <  1:3903480/100‑1 (MQ=255)
                                                                               gttgttATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTGGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTa                 <  1:1307985/100‑1 (MQ=255)
                                                                                 tgttATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTaaa               >  1:3408476/1‑100 (MQ=255)
                                                                                 tgttATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGACTGGTATGGTGGGGCGCTTaaa               >  2:697301/1‑100 (MQ=255)
                                                                                   ttATCCCCAGTTTTGTGGTTGCACCATTATTAGGCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAAtt             <  2:380417/100‑1 (MQ=255)
                                                                                   ttATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAAtt             <  2:3231919/100‑1 (MQ=255)
                                                                                    tATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATTATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTc            <  1:3935020/100‑1 (MQ=255)
                                                                                    tATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTc            >  2:2024802/1‑100 (MQ=255)
                                                                                    tATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTc            <  1:92135/100‑1 (MQ=255)
                                                                                     atacccAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCa           <  1:3167819/97‑1 (MQ=255)
                                                                                      tCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCAt          <  1:1564177/100‑1 (MQ=255)
                                                                                       ncccAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCAATGGCTGCCGGGCGGTAGCTGGAATGGTGGGGCGCTTAAATTCATg         >  1:1223149/2‑100 (MQ=255)
                                                                                       ccccAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATg         <  2:2223977/100‑1 (MQ=255)
                                                                                       ccccAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATg         >  2:4096379/1‑100 (MQ=255)
                                                                                       ccccAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATg         >  2:3337835/1‑100 (MQ=255)
                                                                                       ccccAGTTTGGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCAGCCGGGCAGTGCTTGGAATGGTTGGGCGTTTAAAGTCa           >  2:2632168/1‑98 (MQ=255)
                                                                                        cccAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCAt          >  2:3397635/1‑98 (MQ=255)
                                                                                        cccAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGa        >  2:2152886/1‑100 (MQ=255)
                                                                                        cccAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGa        >  1:660699/1‑100 (MQ=255)
                                                                                            gTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATAtt    >  1:3748939/1‑100 (MQ=255)
                                                                                              tttGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGc  <  2:3403249/100‑1 (MQ=255)
                                                                                                  |                                                                                               
TGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGC  >  NC_000913/1303234‑1303427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: