Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,401,718 A→C 56.3% V19G (GTC→GGC)  abgT ← p‑aminobenzoyl‑glutamate transporter; membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,401,7180AC56.3% 44.1 / 138.2 105V19G (GTC→GGC) abgTp‑aminobenzoyl‑glutamate transporter; membrane protein
Reads supporting (aligned to +/- strand):  ref base A (22/24);  new base C (28/31);  total (50/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.34e-01

Only 100 of 110 total aligned reads displayed.

ACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTA  >  NC_000913/1401619‑1401817
                                                                                                   |                                                                                                   
aCGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGa                                                                                                     <  1:1590654/100‑1 (MQ=255)
   ccAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGcccc                                                                                                  >  1:1282678/1‑100 (MQ=255)
   ccAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGcccc                                                                                                  >  1:3378224/1‑100 (MQ=255)
   ccAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGcccc                                                                                                  >  1:1823823/1‑100 (MQ=255)
       aGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGcc                                                                                              <  1:1202733/100‑1 (MQ=255)
           cGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATag                                                                                          >  1:561657/1‑100 (MQ=255)
            gACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATaga                                                                                         <  2:485810/100‑1 (MQ=255)
            gACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTGTCGACCCAGCCATaga                                                                                         >  1:1219486/1‑100 (MQ=255)
             aCAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATagag                                                                                        >  1:997806/1‑100 (MQ=255)
              cAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGc                                                                                       <  1:1098991/100‑1 (MQ=255)
              cAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGc                                                                                       <  2:1298363/100‑1 (MQ=255)
              cAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAACCATAGAGc                                                                                       >  2:3533271/1‑100 (MQ=255)
              cAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGc                                                                                       >  2:859578/1‑100 (MQ=255)
                    tGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCg                                                                                 <  1:1990702/100‑1 (MQ=255)
                     gCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCgg                                                                                <  2:2088203/100‑1 (MQ=255)
                      ccGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGa                                                                               >  2:3096151/1‑100 (MQ=255)
                        gtcgtcACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGAtt                                                                             >  2:888661/1‑100 (MQ=255)
                          cgtcACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCACAGCCATAGAGCTTCCCGGATTg                                                                            >  2:1407331/1‑99 (MQ=255)
                          cgtcACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTCTTACCAATTCTTTCGACCCAGCCATAGATCTTCCCGGAttag                                                                           <  1:2801353/100‑3 (MQ=255)
                           gtcACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTggg                                                                          >  1:748101/1‑100 (MQ=255)
                            tcACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGgga                                                                         <  1:702112/100‑1 (MQ=255)
                            tcACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGgga                                                                         >  1:1459296/1‑100 (MQ=255)
                             cACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTTGCCCCAGCCATAGAGCTCCCCGGATTGggag                                                                        <  1:2785403/100‑1 (MQ=255)
                             cACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGggag                                                                        <  1:3178309/100‑1 (MQ=255)
                             cACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGggag                                                                        >  1:339951/1‑100 (MQ=255)
                              aCCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGggagg                                                                       <  1:622450/100‑1 (MQ=255)
                               ccATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGggagga                                                                      >  1:472362/1‑100 (MQ=255)
                                cATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGgac                                                                     <  1:2831657/100‑1 (MQ=255)
                                cATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGgac                                                                     <  2:280628/100‑1 (MQ=255)
                                 aTGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAGCCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGgac                                                                     <  1:2503169/99‑1 (MQ=255)
                                   gAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGgacgac                                                                  >  1:3732898/1‑100 (MQ=255)
                                       aCGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTa                                                              >  1:2036596/1‑100 (MQ=255)
                                       aCGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTa                                                              >  2:2489264/1‑100 (MQ=255)
                                        cGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTAt                                                             >  1:309990/1‑100 (MQ=255)
                                        cGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTAt                                                             >  2:31736/1‑100 (MQ=255)
                                         gATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATg                                                            <  1:3096170/100‑1 (MQ=255)
                                         gATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATg                                                            <  1:3101843/100‑1 (MQ=255)
                                           tAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGa                                                          >  1:3869646/1‑100 (MQ=255)
                                           tAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGa                                                          >  2:3169674/1‑100 (MQ=255)
                                             aTCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATg                                                        >  2:533753/1‑100 (MQ=255)
                                             aTCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGCATg                                                        <  2:792306/100‑1 (MQ=255)
                                                aaaTAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACa                                                     >  2:2824376/1‑100 (MQ=255)
                                                aaaTAGATAAAGAGCAAAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGa                                                       <  1:1443998/98‑1 (MQ=255)
                                                 aaTAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACAt                                                    <  2:1717775/100‑1 (MQ=255)
                                                   tAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGCCGACGGTATGGATGACATAc                                                  >  2:307869/1‑100 (MQ=255)
                                                    aGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATagt                                                 <  1:2789259/100‑3 (MQ=255)
                                                    aGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACt                                                 <  2:3374772/100‑1 (MQ=255)
                                                     gagaaaGAGCTGAAAAGGATGGGGAAGCTCGTTACCAATTCTCTCGACCCAGCCATATAGCTTCCCGTATTCTTAGGCCGACGGCATGGATGACATAc                                                  >  2:2507875/4‑98 (MQ=255)
                                                       tAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCAt                                              >  1:1655840/1‑100 (MQ=255)
                                                        aaaGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATg                                             <  2:624845/100‑1 (MQ=255)
                                                         aaGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATgg                                            <  1:1897745/100‑1 (MQ=255)
                                                          agagCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATggg                                           <  1:724647/100‑1 (MQ=255)
                                                          agagCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATggg                                           <  1:3341350/100‑1 (MQ=255)
                                                          agagCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATggg                                           <  2:459811/100‑1 (MQ=255)
                                                            agCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGAGTGACATACTCATGTGCa                                         >  2:2763003/1‑100 (MQ=255)
                                                             gCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCAt                                        >  1:374437/1‑100 (MQ=255)
                                                              cAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGAATGGGAGGACGACGGTATGGATGACATACTCATGGGCAtt                                       >  2:1510808/1‑100 (MQ=255)
                                                              cAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCAtt                                       <  1:819410/100‑1 (MQ=255)
                                                                gAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTcc                                     >  2:2282120/1‑100 (MQ=255)
                                                                gAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCGTTcc                                     >  1:116700/1‑100 (MQ=255)
                                                                  aaaGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTc                                   <  2:3863766/100‑1 (MQ=255)
                                                                  aaaGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTc                                   >  2:313737/1‑100 (MQ=255)
                                                                  aaaGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTc                                   <  2:3057720/100‑1 (MQ=255)
                                                                   aaGGATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCg                                  <  1:1718319/100‑1 (MQ=255)
                                                                    aGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCgg                                 >  2:3239760/1‑100 (MQ=255)
                                                                     ggATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGgt                                <  1:972599/100‑1 (MQ=255)
                                                                     ggATGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGgt                                >  2:2962538/1‑100 (MQ=255)
                                                                     ggATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCgg                                 >  2:2834858/1‑99 (MQ=255)
                                                                     ggATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGgt                                >  1:4080279/1‑100 (MQ=255)
                                                                       aTGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCgg                                 <  2:226653/97‑1 (MQ=255)
                                                                       aTGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGgttg                              <  2:2943320/100‑1 (MQ=255)
                                                                       aTGGGGAACCTTGTTACCAATTCTTGCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGgttg                              <  2:514353/100‑1 (MQ=255)
                                                                        tGGGGAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGgttgt                             >  1:890081/1‑100 (MQ=255)
                                                                         ggggAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGgttgtt                            >  1:2016066/1‑100 (MQ=255)
                                                                         ggggAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGgttgtt                            >  2:1291821/1‑100 (MQ=255)
                                                                          gggAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGttgttg                           <  2:3416683/100‑1 (MQ=255)
                                                                          gggAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGttgttg                           <  1:2402017/100‑1 (MQ=255)
                                                                          gggAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGttgttg                           <  1:778082/100‑1 (MQ=255)
                                                                           ggAACCTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTtgttgt                          <  1:3600356/100‑1 (MQ=255)
                                                                            gAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTtgtg                         <  1:2113963/100‑1 (MQ=255)
                                                                                cTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTg                     <  2:781663/100‑1 (MQ=255)
                                                                                      aCCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCgttgt               >  1:1105301/1‑100 (MQ=255)
                                                                                      aCCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCgttgt               >  2:1181991/1‑100 (MQ=255)
                                                                                      aCCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCgttgt               <  2:454254/100‑1 (MQ=255)
                                                                                        cAATTCTTTCGCCCCAGCCATAGAGTTGCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTGGTTGTGTTTGCgttgttg             <  1:307872/100‑1 (MQ=255)
                                                                                        cAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCgttgttg             >  1:833836/1‑100 (MQ=255)
                                                                                        cAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCgttgttg             <  2:4036402/100‑1 (MQ=255)
                                                                                        cAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCgttgttg             <  1:3519035/100‑1 (MQ=255)
                                                                                         aaTTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCgttgttgt            <  1:1348654/100‑1 (MQ=255)
                                                                                           ttCTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCgttgttgttg          <  2:2332721/100‑1 (MQ=255)
                                                                                           ttCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCgttgttgttg          <  2:3509203/100‑1 (MQ=255)
                                                                                             cTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCgttgttgttgtt        <  2:2621494/100‑1 (MQ=255)
                                                                                             cTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCgttgttgttgtt        >  1:3636722/1‑100 (MQ=255)
                                                                                             cTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCgttgttgttgtt        <  2:1036265/100‑1 (MQ=255)
                                                                                             cTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCgttgttgttgtt        >  1:2017537/1‑100 (MQ=255)
                                                                                             cTTTCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCgtggttgttgtt        >  1:1461293/1‑100 (MQ=255)
                                                                                               ttCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTAt      >  2:1120589/1‑100 (MQ=255)
                                                                                               ttCGCCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTAt      >  2:2983449/1‑100 (MQ=255)
                                                                                                  gACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGCTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTAtttt   >  2:2556193/1‑100 (MQ=255)
                                                                                                   aCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTa  <  2:2222303/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
ACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTA  >  NC_000913/1401619‑1401817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: