Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,401,844 A→G 100% I471T (ATC→ACC)  abgB ← p‑aminobenzoyl‑glutamate hydrolase, B subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,401,8440AG98.4% 404.9 / ‑3.0 120I471T (ATC→ACC) abgBp‑aminobenzoyl‑glutamate hydrolase, B subunit
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (58/59);  total (59/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 120 total aligned reads displayed.

GACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTT  >  NC_000913/1401748‑1401939
                                                                                                |                                                                                               
gacgacGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAg                                                                                              <  1:1781979/100‑1 (MQ=255)
gacgacGGTATGGATGACATACTCATGGGCATTCCTCGGTGGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAg                                                                                              <  2:1200327/100‑1 (MQ=255)
 acgacgGTATGGATGACATACTCATGGGCATTACTCGGTTGTTGTGTATGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAgg                                                                                             >  2:1683735/1‑100 (MQ=255)
  cgacgGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAggg                                                                                            >  1:2661186/1‑100 (MQ=255)
  cgacgGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAggg                                                                                            >  1:131456/1‑100 (MQ=255)
  cgacgGTATGGATGACATACTCATGGGCATTCCGCGGTTGTTGGGTTTGCGCTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAggg                                                                                            >  1:822475/1‑100 (MQ=255)
   gacgGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGc                                                                                           <  2:1459286/100‑1 (MQ=255)
     cgGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGc                                                                                           <  2:1323568/98‑1 (MQ=255)
      ggTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGt                                                                                        >  1:1458730/1‑100 (MQ=255)
      ggTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGt                                                                                        <  1:2979514/100‑1 (MQ=255)
      ggTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGt                                                                                        <  1:3879704/100‑1 (MQ=255)
       gTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTg                                                                                       >  2:2325782/1‑100 (MQ=255)
         aTGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGAt                                                                                     >  1:3980520/1‑100 (MQ=255)
           ggATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATAc                                                                                   <  1:437092/100‑1 (MQ=255)
           ggATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATAc                                                                                   >  2:2870392/1‑100 (MQ=255)
           ggATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATAc                                                                                   <  1:1319880/100‑1 (MQ=255)
           ggATGACATACTCATGGGCATTCCTCGGTTGTTGTGCTTGCGTTGTTGTTGTTCTTTTAAAGTTGACGGTGTCACGTTTTTCGGGGTACGGCAGTGATAc                                                                                   >  1:780947/1‑100 (MQ=255)
            gATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACg                                                                                  >  2:861351/1‑100 (MQ=255)
              tGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGt                                                                                <  1:2489278/100‑1 (MQ=255)
                  aTACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCg                                                                            <  1:3335527/100‑1 (MQ=255)
                  aTACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCg                                                                            <  2:3834532/100‑1 (MQ=255)
                   tACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCgt                                                                           >  1:2381189/1‑100 (MQ=255)
                    aCTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCgtg                                                                          <  1:2200017/100‑1 (MQ=255)
                     cTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCgtgt                                                                         >  2:3141610/1‑100 (MQ=255)
                     cTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCgtgt                                                                         >  1:1020271/1‑100 (MQ=255)
                     cTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCgtgt                                                                         >  2:161706/1‑100 (MQ=255)
                     cTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCgtgt                                                                         <  2:472358/100‑1 (MQ=255)
                         tGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGt                                                                     >  1:31911/1‑100 (MQ=255)
                           ggCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTa                                                                   >  2:477020/1‑100 (MQ=255)
                           ggCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTa                                                                   >  1:205902/1‑100 (MQ=255)
                            gCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTAc                                                                  >  1:1618269/1‑100 (MQ=255)
                             cATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACt                                                                 >  1:1864643/1‑100 (MQ=255)
                             cATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACt                                                                 <  1:3378050/100‑1 (MQ=255)
                               ttCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTg                                                               >  1:489285/1‑100 (MQ=255)
                               ttCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTg                                                               >  1:488440/1‑100 (MQ=255)
                               ttCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTg                                                               >  1:3836106/1‑100 (MQ=255)
                                 ccTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCt                                                             >  2:3350693/1‑100 (MQ=255)
                                  cTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTg                                                            >  1:811190/1‑100 (MQ=255)
                                   tCGGTTTTTGTTTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTTCGTGTCCGTTACTTGCTg                                                            <  1:4088004/99‑1 (MQ=255)
                                   tCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGa                                                           <  2:2730270/100‑1 (MQ=255)
                                    cGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGAt                                                          <  1:2726081/100‑1 (MQ=255)
                                    cGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGAt                                                          <  2:1823810/100‑1 (MQ=255)
                                     ggTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGAt                                                          <  2:1282670/99‑1 (MQ=255)
                                      gttgttGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGc                                                        <  2:3178512/100‑1 (MQ=255)
                                      gttgttGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGc                                                        <  2:3970494/100‑1 (MQ=255)
                                      gttgttGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGc                                                        <  2:4080252/100‑1 (MQ=255)
                                         gttgtGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGctct                                                     >  1:2123430/1‑100 (MQ=255)
                                         gttgtGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGctct                                                     <  1:2057568/100‑1 (MQ=255)
                                         gttgtGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCATTACTTGCTGATGctct                                                     >  1:200233/1‑100 (MQ=255)
                                           tgtgTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCttg                                                   <  1:3514449/100‑1 (MQ=255)
                                            gtgtTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCttgt                                                  >  2:3143868/1‑100 (MQ=255)
                                             tgtTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCttgtt                                                 <  1:1209232/100‑1 (MQ=255)
                                             tgtTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCttgtt                                                 <  2:2927776/100‑1 (MQ=255)
                                                ttGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGc                                              >  2:3154901/1‑100 (MQ=255)
                                                ttGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGc                                              <  1:3399078/100‑1 (MQ=255)
                                                 tGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCa                                             <  1:1760201/100‑1 (MQ=255)
                                                    gttgttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTc                                          >  2:4022075/1‑100 (MQ=255)
                                                       gttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTg                                       <  2:1991766/100‑1 (MQ=255)
                                                       gttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTg                                       <  2:3980494/100‑1 (MQ=255)
                                                        ttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTg                                       >  2:1280544/1‑99 (MQ=255)
                                                        ttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGc                                      <  1:1181999/100‑1 (MQ=255)
                                                        ttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGc                                      <  2:836024/100‑1 (MQ=255)
                                                         tgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGc                                      >  2:3876619/1‑99 (MQ=255)
                                                          gttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCaa                                    <  1:2325795/100‑1 (MQ=255)
                                                          gttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCaa                                    >  2:258628/1‑100 (MQ=255)
                                                           ttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAAt                                   <  2:1056318/100‑1 (MQ=255)
                                                            tgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCATGCAATa                                  <  1:2173606/100‑1 (MQ=255)
                                                             gttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATa                                  <  2:3284557/99‑1 (MQ=255)
                                                             gttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATa                                  >  1:3531659/1‑99 (MQ=255)
                                                             gttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAg                                 >  1:3235062/1‑100 (MQ=255)
                                                             gttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAg                                 >  1:138665/1‑100 (MQ=255)
                                                               tATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACTGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGcc                               <  2:4072210/100‑1 (MQ=255)
                                                                aTTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGGAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGccc                              <  2:2253543/100‑1 (MQ=255)
                                                                 ttttAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCt                             >  1:429011/1‑100 (MQ=255)
                                                                  tttAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTg                            <  1:2326928/100‑1 (MQ=255)
                                                                    tAAAGGTGACGGTGTCACGTTTTTCGGGTTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGaa                          <  2:2865088/100‑1 (MQ=255)
                                                                     aaaGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAAt                         <  2:339948/100‑1 (MQ=255)
                                                                     aaaGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAAt                         <  1:832801/100‑1 (MQ=255)
                                                                     aaaGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAAt                         <  1:4022101/100‑1 (MQ=255)
                                                                       aGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCa                       >  1:4034254/1‑100 (MQ=255)
                                                                        ggTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCaa                      >  1:1420570/1‑100 (MQ=255)
                                                                        ggTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCaa                      <  2:3271333/100‑1 (MQ=255)
                                                                         gTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAg                     <  1:2600414/100‑1 (MQ=255)
                                                                          tGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAgg                    <  1:1049676/100‑1 (MQ=255)
                                                                          tGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAgg                    >  2:539312/1‑100 (MQ=255)
                                                                              ggTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAaga                <  2:3836081/100‑1 (MQ=255)
                                                                                 gtCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGAt             >  1:2609364/1‑100 (MQ=255)
                                                                                  tCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGAtt            <  1:234174/100‑1 (MQ=255)
                                                                                   cACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTg           >  2:3048998/1‑100 (MQ=255)
                                                                                   cACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTATCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTg           >  1:2917966/1‑100 (MQ=255)
                                                                                     cGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGAc         <  1:31736/100‑1 (MQ=255)
                                                                                        ttttCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACtgt      >  2:321007/1‑100 (MQ=255)
                                                                                        ttttCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACtgt      <  2:2381175/100‑1 (MQ=255)
                                                                                         tttCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTTCTGCTGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGCGCTTGACtgt      >  2:2051315/1‑99 (MQ=255)
                                                                                         tttCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACtgtg     <  1:187086/100‑1 (MQ=255)
                                                                                          ttCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTgg    >  2:1765435/1‑100 (MQ=255)
                                                                                          ttCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTgg    <  2:495876/100‑1 (MQ=255)
                                                                                          ttCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTgg    >  1:1283257/1‑100 (MQ=255)
                                                                                            cGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGtt  >  1:575763/1‑100 (MQ=255)
                                                                                            cGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGtt  <  2:489281/100‑1 (MQ=255)
                                                                                                |                                                                                               
GACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTT  >  NC_000913/1401748‑1401939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: