Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 3,542,354 | Δ1 bp | coding (1948/2322 nt) | feoB → | ferrous iron transporter protein B and GTP‑binding protein; membrane protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,542,351 | 0 | G | . | 96.3% | 236.5 / 2.9 | 54 | coding (1945/2322 nt) | feoB | ferrous iron transporter protein B and GTP‑binding protein; membrane protein |
Reads supporting (aligned to +/- strand): ref base G (2/0); new base . (25/27); total (27/27) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01 |
GATAGATGAAACCTGGCAGAGCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTG > NC_000913/3542254‑3542446 | gATAGATGAAACCTGGCAGAGCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCggg < 1:992942/100‑1 (MQ=255) atcgATGAAACCTGGCAGAGCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCgggc > 1:628271/4‑99 (MQ=255) aTAGATGAAACCTGGCAGAGCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCgggc < 1:1186803/100‑2 (MQ=255) aTGAAACCTGGCAGAGCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATg > 1:1103982/1‑100 (MQ=255) tGAAACCTGGCAGAGCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATgg > 2:942361/1‑100 (MQ=255) naaaCCTGGCAGAGCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATggg > 2:266438/2‑100 (MQ=255) gAAACCTGGCAGAGCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATggg > 2:405099/1‑100 (MQ=255) gAAACCTGGCAGAGCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATggg > 2:720675/1‑100 (MQ=255) aaCCTGGCAGAGCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCg > 1:533762/1‑100 (MQ=255) cTGGCAGAGCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGa < 2:86723/100‑1 (MQ=255) gCAGAGCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATgg < 1:726186/100‑1 (MQ=255) agCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCa < 2:1046233/100‑1 (MQ=255) agCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCa > 1:790426/1‑100 (MQ=255) gCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAg > 1:1234875/1‑100 (MQ=255) gCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAg > 2:569035/1‑100 (MQ=255) gCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAg < 1:57897/100‑1 (MQ=255) gCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAg < 1:57928/100‑1 (MQ=255) gCCTGAAAGACACCTGCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATc < 2:1243031/98‑1 (MQ=255) ccTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGACCAGa > 2:901754/1‑100 (MQ=255) cTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGaa < 1:569039/100‑1 (MQ=255) gAAAGACACCTTCAGCCTCAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGaaa < 2:1123868/99‑1 (MQ=255) gACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGTGATGGATCAGAAATTCg > 2:569676/1‑100 (MQ=255) gACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCgg < 2:32537/100‑1 (MQ=255) cTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAgcg > 2:58091/1‑100 (MQ=255) tCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCgca < 1:683972/100‑1 (MQ=255) cAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCgcag > 2:270412/1‑100 (MQ=255) aGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCgcagc < 1:1152373/100‑1 (MQ=255) aGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCgcagc < 2:783758/100‑1 (MQ=255) gCCTTAGCGTACTGATGAACCCCAGTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCgcagc < 2:602736/99‑1 (MQ=255) ccTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCgcagcag > 1:1059849/1‑100 (MQ=255) ccTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCgcagcag < 1:491304/100‑1 (MQ=255) cTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAg > 1:431549/1‑100 (MQ=255) cTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAg < 1:1131956/100‑1 (MQ=255) atgaACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTa < 2:551447/100‑1 (MQ=255) tgaACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTAc < 2:1304288/100‑1 (MQ=255) aCCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTg > 1:704618/1‑100 (MQ=255) ctaTTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGAtt < 2:793251/98‑1 (MQ=255) aTTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGAttt < 1:270415/99‑1 (MQ=255) ttGAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTc < 2:533758/100‑1 (MQ=255) gaggCCAGCAGAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCg < 2:819246/96‑1 (MQ=255) gAAGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGt > 1:264791/1‑100 (MQ=255) aGCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTcc < 2:435620/100‑1 (MQ=255) gCCAGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCct > 2:974847/1‑100 (MQ=255) aGCAAAGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCctgct > 1:620307/1‑100 (MQ=255) aaaGGCGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCtgta < 2:845717/100‑1 (MQ=255) cGACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTAc > 1:153104/1‑100 (MQ=255) gACGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTAcc > 1:660886/1‑100 (MQ=255) aCGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCa < 1:106824/100‑1 (MQ=255) cGGCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCAt > 1:1188539/1‑100 (MQ=255) ggCGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATg < 2:285082/100‑1 (MQ=255) cGAAATGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTa > 1:255730/1‑100 (MQ=255) aaaTGGGTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTAtc > 2:952565/1‑100 (MQ=255) gggTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCgg > 2:631207/1‑100 (MQ=255) gggTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCgg < 1:780361/100‑1 (MQ=255) ggTACCGGGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCgg > 2:108657/1‑100 (MQ=255) gTACC‑GGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTg > 2:1061251/1‑100 (MQ=255) gTACC‑GGGCGATGGGCGTGATGGATCAGAAACTCGGTAGCGCAGAAGTAGCTTACAGCTACCTGATTTTCCTCCTACTGTATGTACCATGTATCTCGGTg < 2:146922/100‑1 (MQ=255) | GATAGATGAAACCTGGCAGAGCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTG > NC_000913/3542254‑3542446 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |