| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,639,576 | (A)6→5 | coding (145/336 nt) | uspB ← | universal stress (ethanol tolerance) protein B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,639,571 | 0 | A | . | 96.1% | 216.1 / 2.2 | 51 | coding (150/336 nt) | uspB | universal stress (ethanol tolerance) protein B |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base . (22/27); total (23/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01 | |||||||||||
ACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCA > NC_000913/3639472‑3639665 | acaGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTa > 1:2583/1‑100 (MQ=255) caGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTaa > 2:1126525/1‑100 (MQ=255) gcggcgAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTTGCCATGTGAGGTaaaa < 1:998877/100‑1 (MQ=255) gcgAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTaaaaagn < 1:537136/100‑3 (MQ=255) gcgAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTaaaaagc > 1:1203560/1‑98 (MQ=255) cgAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTaaaaagcc > 1:1271534/1‑97 (MQ=255) gAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTaaaaagccc < 1:144295/100‑5 (MQ=255) aaTTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTcc > 1:125761/1‑100 (MQ=255) catcGTGATGATCGCGATAACGTTGGGCATTGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACa < 2:972515/100‑1 (MQ=255) catcGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACATGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACa > 1:1249300/1‑100 (MQ=255) gatgatCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGa > 2:1019718/1‑100 (MQ=255) atgatCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGAt < 2:183352/100‑1 (MQ=255) atgatCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGAt < 2:395062/100‑1 (MQ=255) tgatCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATa > 1:1287854/1‑100 (MQ=255) gatCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATag > 1:389101/1‑100 (MQ=255) gcgATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCa > 2:1195264/1‑100 (MQ=255) cGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATc < 1:68027/100‑1 (MQ=255) tGGGGATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGc < 2:1146641/99‑1 (MQ=255) tGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGc < 2:2583/100‑1 (MQ=255) ggCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAg < 2:1083274/99‑1 (MQ=255) ggCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGt > 1:177269/1‑100 (MQ=255) cATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTa < 1:459236/100‑1 (MQ=255) aGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACg > 1:219678/1‑100 (MQ=255) natatACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACg > 2:671678/2‑98 (MQ=255) natatACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCa > 2:671693/2‑100 (MQ=255) taCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTAc < 2:1003021/100‑1 (MQ=255) aCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTAcc < 1:72758/100‑1 (MQ=255) cAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCAc < 1:623325/100‑1 (MQ=255) cAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCAc > 2:273684/1‑100 (MQ=255) gagaCGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTaaca > 1:17624/1‑100 (MQ=255) agaCGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTaacaa > 2:1170061/1‑100 (MQ=255) agaCGCACCTGTTCGTTGGGTTGGCCATGTGAGGT‑AAAAAGCACCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTaacaa > 1:882874/1‑100 (MQ=255) aCGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcg < 1:937333/100‑1 (MQ=255) aCGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcg < 2:1022368/100‑1 (MQ=255) gCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcgcg < 2:964685/100‑1 (MQ=255) gCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcgcg < 2:601985/100‑1 (MQ=255) cACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcgcgc > 1:244843/1‑100 (MQ=255) cACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGCACCACTAACAAcgcgc > 2:760994/1‑100 (MQ=255) tttgttGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtg < 2:882867/100‑1 (MQ=255) tttgttGGGTTGGCCATGTGAAGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtg < 1:1081420/100‑1 (MQ=255) ttgttgGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAGCATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtga > 2:714383/1‑100 (MQ=255) ttgttgGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAGCATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtga > 2:714290/1‑100 (MQ=255) ttgttgGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtga < 1:1245957/100‑1 (MQ=255) ttgttgGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtga > 1:192702/1‑100 (MQ=255) gttgggttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtgatg < 1:1126535/100‑1 (MQ=255) gttgggttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtgatg > 1:833547/1‑100 (MQ=255) gttgggttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtgatg < 2:790544/100‑1 (MQ=255) gttgggttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtgatg > 2:676176/1‑100 (MQ=255) gttgggttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtgatg > 1:428157/1‑100 (MQ=255) gttgggttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtgatg > 1:689087/1‑100 (MQ=255) gttgggttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtgatg < 2:256090/100‑1 (MQ=255) gggttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATgaga < 1:1126000/100‑1 (MQ=255) ttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAAt < 2:556367/100‑1 (MQ=255) cATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgc < 2:1271523/100‑1 (MQ=255) cATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgc < 1:747407/100‑1 (MQ=255) tgtgAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgcgc < 2:1287843/100‑1 (MQ=255) tgAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCa < 1:734510/100‑1 (MQ=255) tgAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCa < 2:173395/100‑1 (MQ=255) | ACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCA > NC_000913/3639472‑3639665 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |