Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,560,731 Δ1 bp coding (199/1023 nt) ddpB ← D,D‑dipeptide ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,560,7280A.98.2% 245.7 / ‑2.8 55coding (202/1023 nt)ddpBD,D‑dipeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (28/26);  minor base G (1/0);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCG  >  NC_000913/1560630‑1560823
                                                                                                  |                                                                                               
cGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTaa                                                                                                <  2:1184550/100‑1 (MQ=255)
cGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTaa                                                                                                <  1:584512/100‑1 (MQ=255)
  tCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTaaac                                                                                              <  1:535825/100‑2 (MQ=255)
     ccGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTaaactgt                                                                                           >  1:1239703/1‑96 (MQ=255)
       gggAAAAATATACGCGACTCTTCCAGCAGGGTACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGGAAACTGtac                                                                                        >  2:844386/1‑100 (MQ=255)
       gggAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGtac                                                                                        <  1:10798/100‑1 (MQ=255)
        ggAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGtaca                                                                                       <  2:1189895/100‑1 (MQ=255)
         gAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGtacat                                                                                      >  2:547322/1‑100 (MQ=255)
             aaTATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAg                                                                                  <  1:1263125/100‑1 (MQ=255)
                   cGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTg                                                                            <  2:859091/100‑1 (MQ=255)
                   cGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTg                                                                            >  1:21071/1‑100 (MQ=255)
                   cGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTg                                                                            >  2:955352/1‑100 (MQ=255)
                     cAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGt                                                                          >  1:974030/1‑100 (MQ=255)
                           ttCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTc                                                                    <  1:550843/100‑1 (MQ=255)
                             ccagccaccgACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACCTACAGTGGCTGGTCCAGTc                                                                    <  2:821917/89‑1 (MQ=255)
                             ccAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTccc                                                                  >  2:704789/1‑100 (MQ=255)
                               aGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAg                                                                <  2:756507/100‑1 (MQ=255)
                                     ggACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTg                                                          >  1:245971/1‑100 (MQ=255)
                                     ggACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTg                                                          <  1:618332/100‑1 (MQ=255)
                                       aCGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGc                                                        <  2:875267/100‑1 (MQ=255)
                                           ccGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAat                                                    >  1:810100/1‑100 (MQ=255)
                                                 cGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTc                                              >  2:775679/1‑100 (MQ=255)
                                                 cGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTc                                              <  1:556959/100‑1 (MQ=255)
                                                 cGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCACAGTTGCTGGCGAATATTTTc                                              <  1:455858/100‑1 (MQ=255)
                                                  gAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTcc                                             <  2:1239692/100‑1 (MQ=255)
                                                  gAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTcc                                             <  1:678027/100‑1 (MQ=255)
                                                  gAATGGATGTTCCCAGGTCACCATGAAACAGGACGCTGAAGTTGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTcc                                             <  2:916849/100‑1 (MQ=255)
                                                   aaTGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCa                                            >  2:1233279/1‑100 (MQ=255)
                                                   aaTGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATACTTTCCa                                            >  2:466468/1‑100 (MQ=255)
                                                       gATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGAt                                        >  2:1297714/1‑100 (MQ=255)
                                                        aTGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATa                                       <  1:806964/100‑1 (MQ=255)
                                                              ccAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCg                                 >  1:1110499/1‑100 (MQ=255)
                                                              ccAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCg                                 >  1:632165/1‑100 (MQ=255)
                                                               cAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGc                                >  1:693403/1‑100 (MQ=255)
                                                                  gTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCGAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTgg                             >  2:833395/1‑100 (MQ=255)
                                                                  gTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTg                              >  2:438725/1‑99 (MQ=255)
                                                                    cACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGgcg                           <  2:175046/100‑1 (MQ=255)
                                                                     aCCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGgcg                           <  2:1213695/99‑1 (MQ=255)
                                                                      ccATGAAAGACGTCGCTGACGGATCGGTAAACTGTACATACAGTGGCTGGTCCAATCACAGTTGCTGCAGAATCTCTTCCACGATAGCAACGCTGGcgcg                         >  2:69101/1‑100 (MQ=255)
                                                                      ccATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGcgcg                         >  1:270757/1‑100 (MQ=255)
                                                                      ccATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGcgcg                         >  1:187287/1‑100 (MQ=255)
                                                                      ccATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGcgcg                         <  2:1062141/100‑1 (MQ=255)
                                                                       cATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCgg                        <  1:978384/100‑1 (MQ=255)
                                                                       cATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCgg                        <  1:704793/100‑1 (MQ=255)
                                                                       cATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCgg                        <  1:221614/100‑1 (MQ=255)
                                                                         tGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTc                      <  2:810095/100‑1 (MQ=255)
                                                                           aaaCAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAAAATTTTCCACGATAGCATCGCTGGCGCGGTCa                     >  2:459123/1‑99 (MQ=255)
                                                                            aaCAGGGCGCTGACGTAGCGGTGAACTGTACATACAGTGGGTGGACGAGTCCCAGTGGCGTGGGTATGTGTTGGACGAGAGCATCGCTGTcgcg                         >  2:1167850/1‑94 (MQ=255)
                                                                               aGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGc                <  1:151619/100‑1 (MQ=255)
                                                                               aGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGc                <  1:547326/100‑1 (MQ=255)
                                                                                ggTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTTGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGcc               >  2:397695/1‑100 (MQ=255)
                                                                                ggTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGcc               >  1:269959/1‑100 (MQ=255)
                                                                                ggTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGcc               >  2:9123/1‑100 (MQ=255)
                                                                                  tCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAg             >  2:982044/1‑100 (MQ=255)
                                                                                   cGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGt            >  2:142957/1‑100 (MQ=255)
                                                                                   cGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGt            >  1:1094294/1‑100 (MQ=255)
                                                                                        aCGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACcg       <  2:664306/100‑1 (MQ=255)
                                                                                           tAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCgcgc    <  1:1296765/100‑1 (MQ=255)
                                                                                             gCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCg  >  2:60368/1‑100 (MQ=255)
                                                                                                  |                                                                                               
CGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCG  >  NC_000913/1560630‑1560823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: