Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,712,518 (C)6→5 intergenic (+360/‑251) nth → / → dtpA DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)/dipeptide and tripeptide permease A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,712,5130C.98.0% 219.3 / ‑2.2 51intergenic (+355/‑256)nth/dtpADNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)/dipeptide and tripeptide permease A
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base . (23/26);  minor base T (0/1);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCT  >  NC_000913/1712415‑1712606
                                                                                                  |                                                                                             
atTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAcc                                                                                              >  2:593773/1‑100 (MQ=255)
  tGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAcccc                                                                                            >  2:416307/1‑100 (MQ=255)
   gACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAccccc                                                                                           >  2:96161/1‑100 (MQ=255)
   gACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAccccc                                                                                           <  2:1275557/100‑1 (MQ=255)
      cTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAccccctct                                                                                        <  1:650655/100‑4 (MQ=255)
       tACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAccccctctc                                                                                       <  2:390593/100‑5 (MQ=255)
         cAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcag                                                                                    >  1:1147641/1‑100 (MQ=255)
            aaCATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagtgc                                                                                 <  1:70959/100‑1 (MQ=255)
               aTTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagtgcagt                                                                              <  1:91398/100‑1 (MQ=255)
                          tATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATAACGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATc                                                                     <  2:1259103/98‑1 (MQ=255)
                            tttttCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGcc                                                                 <  2:651387/100‑1 (MQ=255)
                            tttttCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGcc                                                                 >  1:1280821/1‑100 (MQ=255)
                              tttCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGt                                                               >  1:1130080/1‑100 (MQ=255)
                              tttCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGt                                                               <  1:1210707/100‑1 (MQ=255)
                               ttCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGtt                                                              <  2:1067642/100‑1 (MQ=255)
                                 cAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTAc                                                            >  1:493917/1‑100 (MQ=255)
                                 cAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTAc                                                            >  2:378974/1‑100 (MQ=255)
                                  aGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACg                                                           <  2:144135/100‑1 (MQ=255)
                                     aaCTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGttt                                                        >  2:675698/1‑100 (MQ=255)
                                      aCTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGtttt                                                       <  1:675353/100‑1 (MQ=255)
                                        tGGACTAGCTCACTAGTAAAATCGATAAATAGCAGAACATATAGCCGTTAAGCCATCTTCCCCTTTAAAAAAAAAAAAAAAATCTGCCGTTACGtttttt                                                     <  2:711320/100‑1 (MQ=255)
                                         ggACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTg                                                    >  1:1277297/1‑100 (MQ=255)
                                         ggACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTg                                                    >  1:622811/1‑100 (MQ=255)
                                         ggACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTg                                                    >  1:68664/1‑100 (MQ=255)
                                         ggACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTg                                                    <  2:577025/100‑1 (MQ=255)
                                             tATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGaaaa                                                <  1:96163/100‑1 (MQ=255)
                                              aTCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGaaaaa                                               >  1:109666/1‑100 (MQ=255)
                                                ctcACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAAtt                                             >  2:938623/1‑100 (MQ=255)
                                                ctcACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAAtt                                             >  1:451086/1‑100 (MQ=255)
                                                 tcACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAAttt                                            >  2:952350/1‑100 (MQ=255)
                                                  cACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTa                                           >  1:208190/1‑100 (MQ=255)
                                                  cACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTa                                           <  2:1112623/100‑1 (MQ=255)
                                                  cACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTa                                           >  1:684308/1‑100 (MQ=255)
                                                    cTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTACAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAAc                                         <  2:674005/100‑1 (MQ=255)
                                                         aaaaaCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTgg                                    >  1:62592/1‑100 (MQ=255)
                                                            aaCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATa                                 <  1:378977/100‑1 (MQ=255)
                                                             aCGCTAAATCGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATa                                 <  1:682781/99‑1 (MQ=255)
                                                              cGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAAc                               >  1:1056943/1‑100 (MQ=255)
                                                               gCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACa                              <  2:1250171/100‑1 (MQ=255)
                                                               gCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACa                              <  2:1130070/100‑1 (MQ=255)
                                                                cTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACAt                             <  1:424235/100‑1 (MQ=255)
                                                                cTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACAt                             >  1:1223101/1‑100 (MQ=255)
                                                                  aaaTAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACAttt                           <  1:84331/100‑1 (MQ=255)
                                                                   aaTAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTc                          <  1:653726/100‑1 (MQ=255)
                                                                       gCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCgg                      >  2:1293283/1‑100 (MQ=255)
                                                                       gCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCgg                      <  1:7327/100‑1 (MQ=255)
                                                                       gCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAAGTTAACGCTGGATAACATTTCCCgg                      >  2:385806/1‑100 (MQ=255)
                                                                              atatCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGtt               <  1:865684/100‑1 (MQ=255)
                                                                               tatCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTg              >  1:538168/1‑100 (MQ=255)
                                                                               tatCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTg              <  1:18386/100‑1 (MQ=255)
                                                                               tatCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTg              <  1:957177/100‑1 (MQ=255)
                                                                                  cGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAAt           <  2:625338/100‑1 (MQ=255)
                                                                                     cGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTcc        <  2:62591/100‑1 (MQ=255)
                                                                                        tAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTcccc      >  1:927009/1‑100 (MQ=255)
                                                                                        tAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATATGCCGTGACGTTTTTAGAAAAATTTAACGCTGGATAACATTTCCCGGGATGGTTGAATACACCg     >  2:1273518/1‑100 (MQ=255)
                                                                                         aaGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGc    <  1:952358/100‑1 (MQ=255)
                                                                                           gCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCAGCCt  >  1:590716/1‑100 (MQ=255)
                                                                                                  |                                                                                             
ATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCT  >  NC_000913/1712415‑1712606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: