| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,870,998 | (T)6→5 | coding (614/1476 nt) | yeaI → | putative membrane‑anchored diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,870,993 | 0 | T | . | 96.5% | 248.4 / 2.0 | 58 | coding (609/1476 nt) | yeaI | putative membrane‑anchored diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base . (29/27); total (30/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01 | |||||||||||
TTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGC > NC_000913/1870896‑1871088 | tttGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCttt > 2:718667/1‑100 (MQ=255) ttGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCtttt < 2:459687/100‑1 (MQ=255) tGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCttttt > 1:442479/1‑100 (MQ=255) tGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCttttt > 1:216217/1‑100 (MQ=255) gattAACCTCGCTGGCGCTCTTTTTTTATGGAAAAGACAACATCCTTTACAACAATAAG‑AAAATCACGGGAATCCTGTTGCTGGCGCTGATCCCttttt < 2:617864/97‑1 (MQ=255) tttAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACGACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCtttttag > 2:699016/1‑98 (MQ=255) tttAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCtttttag > 1:68236/1‑98 (MQ=255) ccTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGttttt > 1:451550/1‑100 (MQ=255) cGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTccc < 1:1017583/100‑1 (MQ=255) gCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTcctc > 2:793428/1‑98 (MQ=255) cTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTccccn < 1:1098271/100‑2 (MQ=255) gcTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTgg < 1:721716/100‑1 (MQ=255) gcTCTTTTGTTATGGAAAAGACAACATCCTTGAAAAAAAAAAA‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTgg < 2:364531/100‑1 (MQ=255) ctctTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGc < 2:969841/100‑1 (MQ=255) ctctTTTGTTATGGAAAAGACAACATCCTTAACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGc < 1:46262/100‑1 (MQ=255) ctTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGca > 1:981630/1‑100 (MQ=255) ctTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGccc > 2:585989/1‑98 (MQ=255) ctTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGcac > 1:830269/1‑100 (MQ=255) ctTTTGTTATGGAAAAGACAACATCCTTGACAAAAAAAAA‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGc < 2:1064391/98‑1 (MQ=255) ttGTTATGGAAAAGACAACATCCTTGACAACAATATA‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGcacaca < 2:289225/100‑1 (MQ=255) tGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACaa > 2:888156/1‑100 (MQ=255) gTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAAt > 1:322718/1‑100 (MQ=255) ttATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATc < 2:559943/100‑1 (MQ=255) ttATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATc < 2:303137/100‑1 (MQ=255) aTGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCtn < 2:528238/100‑2 (MQ=255) aTGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTg > 2:275913/1‑100 (MQ=255) ggAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAg > 1:404105/1‑100 (MQ=255) gAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACACTCTGAGc > 2:1110106/1‑100 (MQ=255) gAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGc > 1:29694/1‑100 (MQ=255) aaaGACAACCTCCTTGACAACAATAAGAAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCa < 1:572383/100‑1 (MQ=255) aGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGtt < 1:720131/100‑1 (MQ=255) gACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGtt > 1:351377/1‑99 (MQ=255) gACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTta < 1:1050681/100‑1 (MQ=255) gACAACAACCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTta < 2:1134168/100‑1 (MQ=255) acaacaTCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTtat > 1:713440/1‑100 (MQ=255) tCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCt > 1:15851/1‑100 (MQ=255) tCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAACCTGAGCAGTTATAACGCt > 1:674233/1‑100 (MQ=255) ttGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGAc < 2:163470/100‑1 (MQ=255) acaacaATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTAt > 2:415566/1‑100 (MQ=255) aaaacaaAAAA‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTa < 2:101989/97‑1 (MQ=255) aacaaTAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTc < 1:608864/100‑1 (MQ=255) acaaTAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCt > 1:1104030/1‑100 (MQ=255) aTAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTg < 2:1064957/100‑1 (MQ=255) aTAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTg < 2:1171266/100‑1 (MQ=255) aaG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTa < 1:1030626/100‑1 (MQ=255) aaG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTa > 2:1030617/1‑100 (MQ=255) aG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTAt > 2:909716/1‑100 (MQ=255) aG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTAt > 1:1086977/1‑100 (MQ=255) g‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATg > 1:1063830/1‑100 (MQ=255) g‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATg < 1:925262/100‑1 (MQ=255) g‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATg > 2:587740/1‑100 (MQ=255) g‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATg < 1:1133054/100‑1 (MQ=255) acaaaaCGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATgtc > 1:882618/3‑100 (MQ=255) aaaaaaCGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATgtc > 2:1187199/1‑100 (MQ=255) aaaaaaCGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATgtc > 2:88945/1‑100 (MQ=255) gAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTAc > 2:172352/1‑100 (MQ=255) gAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTAc > 2:913250/1‑100 (MQ=255) cTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTcc < 2:1117019/100‑1 (MQ=255) tGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTcc < 2:981622/100‑1 (MQ=255) ttGCTGGCGTTGATCCC‑TTTATCGTATCTGCTCTTCTCGCACACACTCTCAGACGTACTTACGCTGACTATTCTTTCTATTTCGACGATCACTGTCCg < 2:760480/98‑1 (MQ=255) gCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACa > 1:562084/1‑100 (MQ=255) tGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAAc > 2:477466/1‑100 (MQ=255) cgcTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCAt > 1:368232/1‑100 (MQ=255) gATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTg < 2:351374/100‑1 (MQ=255) aTCCC‑TTTTTAGTTTTTCCTCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGc < 2:442475/100‑1 (MQ=255) | TTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGC > NC_000913/1870896‑1871088 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |