Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,469,656	(T)7→8	coding (526/1332 nt)	gtrS →	serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,469,649	1	.	T	100.0%	238.7 / NA	68	L173L (CTA→CTT)	gtrS	serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (25/43);  total (25/43)

ATGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑TTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACA  >  NC_000913/2469551‑2469741                                                                                                    |                                                                                             aTGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGCGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑t                                                                                             <  1:1266659/100‑1 (MQ=255)   gTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATAAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑ttt                                                                                           <  2:698601/100‑1 (MQ=255)       gCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑ttttttt                                                                                       <  1:17576/100‑1 (MQ=255)        catcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑tttttttt                                                                                      >  2:274502/1‑99 (MQ=255)         atcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑ttttttttc                                                                                     <  1:35470/100‑3 (MQ=255)           catTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑ttttttttcgt                                                                                   <  1:1239139/100‑5 (MQ=255)           catTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑ttttttttcgt                                                                                   <  1:1130801/100‑5 (MQ=255)            atTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTTTTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑ttttttttcttt                                                                                  >  2:399657/1‑95 (MQ=255)             ttCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTg                                                                                  <  1:647831/100‑1 (MQ=255)             ttCTATCAAGAGCAAATAGTTATAATGTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTg                                                                                  >  1:791839/1‑100 (MQ=255)               cTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCt                                                                                >  1:78075/1‑100 (MQ=255)               cTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCt                                                                                <  2:745122/100‑1 (MQ=255)                tATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCta                                                                               >  1:1021335/1‑100 (MQ=255)                 aTCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaa                                                                              >  1:304390/1‑100 (MQ=255)                    aaGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaataa                                                                           >  1:1151478/1‑100 (MQ=255)                     agagCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTaataat                                                                          <  2:1284636/100‑1 (MQ=255)                      gagCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAataata                                                                         >  1:944402/1‑100 (MQ=255)                      gagCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAataata                                                                         <  1:410506/100‑1 (MQ=255)                           aataatTATAATTTTCCTTCCATTTTTTTTGTATTAAAAAAAAAAAAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTaataat                                                                          <  2:263445/94‑1 (MQ=255)                           aaTAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTAc                                                                    <  1:933615/100‑1 (MQ=255)                             tAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTg                                                                  <  2:220858/100‑1 (MQ=255)                                ttATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATTCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGc                                                                 <  2:327764/98‑1 (MQ=255)                                ttATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTa                                                               <  1:115676/100‑1 (MQ=255)                                 tataATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTaa                                                              <  1:609869/100‑1 (MQ=255)                                      gatctcttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATc                                                         <  1:182950/95‑1 (MQ=255)                                       tttCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCa                                                        <  2:1891/100‑1 (MQ=255)                                       tttCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCa                                                        >  2:1161175/1‑100 (MQ=255)                                        ttccttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCTTTTCTAATAATATCTACTGCTAAAAATCaa                                                       >  2:112899/1‑100 (MQ=255)                                         tccttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAt                                                      >  2:508702/1‑100 (MQ=255)                                         tccttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAt                                                      >  2:879214/1‑100 (MQ=255)                                           cttccATTTTTGTTGTATTCAAAAAAAAAAAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAttt                                                    <  2:950342/100‑1 (MQ=255)                                           cttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCagatc                                                    >  2:182948/1‑96 (MQ=255)                                           cttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAttt                                                    >  2:1124363/1‑100 (MQ=255)                                            ttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTta                                                   >  1:954212/1‑100 (MQ=255)                                            ttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTta                                                   <  1:321355/100‑1 (MQ=255)                                              ccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTtata                                                 <  2:184718/100‑1 (MQ=255)                                              ccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTcata                                                 <  1:166515/100‑1 (MQ=255)                                               cATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCTTTGCTAATAATATCTACTGCTAAAAATCAATTTatat                                                >  2:236130/1‑100 (MQ=255)                                               cATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTatat                                                <  1:662344/100‑1 (MQ=255)                                               cATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTatat                                                <  2:1263769/100‑1 (MQ=255)                                                   tttGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAc                                            <  2:1234506/100‑1 (MQ=255)                                                     tGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAccc                                          <  2:713921/100‑1 (MQ=255)                                                      ggTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAcccc                                         <  1:1113460/100‑1 (MQ=255)                                                      ggTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAcccc                                         <  2:78074/100‑1 (MQ=255)                                                      ggTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAcccc                                         <  1:1113739/100‑1 (MQ=255)                                                       gtgtATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCa                                        >  2:939687/1‑100 (MQ=255)                                                           aTTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTaa                                    >  1:324571/1‑100 (MQ=255)                                                           aTTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTaa                                    >  1:374802/1‑100 (MQ=255)                                                           aTTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTaa                                    >  2:491898/1‑100 (MQ=255)                                                              cATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTGACCCCACTAATAg                                 <  1:876713/100‑1 (MQ=255)                                                              cATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAg                                 <  1:1064743/100‑1 (MQ=255)                                                              cATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAg                                 >  1:1289917/1‑100 (MQ=255)                                                                ttAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCTTTTTTAATAATATCTACTGCTAAAAATTAATTTATATTAACTCCACTAATAgt                                >  2:1128522/1‑99 (MQ=255)                                                                 tAACATGCAAAAACAATAAATCTATGCTTTTGATATTTTTTTTCGTTTCTAATAAAAAAAAAAAAAAAAAATCAATTTATATTAACCCCACTAATAgtg                               <  2:1297532/99‑1 (MQ=255)                                                                  aaCATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTa                             <  1:570202/100‑1 (MQ=255)                                                                   aCATGAAAAAACAATAAATCTATTCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTAt                            <  2:1238017/100‑1 (MQ=255)                                                                     aTGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTc                          >  1:1159922/1‑100 (MQ=255)                                                                      tgtaaaaaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCa                         <  2:158186/97‑1 (MQ=255)                                                                      tGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCa                         <  2:550133/100‑1 (MQ=255)                                                                      tGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCa                         <  1:1155158/100‑1 (MQ=255)                                                                         aaaaaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatat                      <  1:622991/100‑1 (MQ=255)                                                                         aaaaaCAATAAATCTATGCTTTCGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatat                      >  1:830516/1‑100 (MQ=255)                                                                               aaTAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatattatatt                >  1:819914/1‑100 (MQ=255)                                                                                aTAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAttt               <  1:1161186/100‑1 (MQ=255)                                                                                     tCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttt          <  2:1289906/100‑1 (MQ=255)                                                                                      cTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTg         <  1:532499/100‑1 (MQ=255)                                                                                      cTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTg         <  2:902482/100‑1 (MQ=255)                                                                                      cTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTg         <  2:131110/100‑1 (MQ=255)                                                                                         tGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGAt       <  2:817731/99‑1 (MQ=255)                                                                                          gCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAg     <  2:944394/100‑1 (MQ=255)                                                                                          gCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAg     <  2:135357/100‑1 (MQ=255)                                                                                           cTTTTGCTATTTTTTTTTTTTTTTTATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTTAt       >  2:1249452/1‑97 (MQ=255)                                                                                           cTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAg     <  2:1294641/99‑1 (MQ=255)                                                                                           cTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGa    <  2:1151467/100‑1 (MQ=255)                                                                                            ttttGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGa    >  2:891218/1‑99 (MQ=255)                                                                                             tttGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGaca  <  2:696653/100‑1 (MQ=255)                                                                                                    |                                                                                             ATGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTA‑TTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACA  >  NC_000913/2469551‑2469741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑