Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,563,518 (A)5→6 intergenic (+401/‑74) yffP → / → yffQ CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,563,5131.A98.0% 171.1 / ‑3.1 49intergenic (+396/‑79)yffP/yffQCPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base A (29/19);  total (30/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

ATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCG  >  NC_000913/2563420‑2563607
                                                                                               |                                                                                              
aTTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaaaaaa                                                                                          <  1:758636/100‑2 (MQ=255)
aTTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaaaaaa                                                                                          >  2:1185745/1‑99 (MQ=255)
 ttttGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaaaaaat                                                                                         <  2:1044231/100‑3 (MQ=255)
  tttGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaaaaaatg                                                                                        <  2:1028815/100‑4 (MQ=255)
   ttGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaaaaaatga                                                                                       <  2:395743/100‑5 (MQ=255)
   ttGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaaaaaatga                                                                                       >  2:251562/1‑96 (MQ=255)
            aGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTggn                                                                               <  2:71364/100‑2 (MQ=255)
             gATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGa                                                                              >  1:311552/1‑100 (MQ=255)
             gATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGa                                                                              >  1:447757/1‑100 (MQ=255)
              aTCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGaa                                                                             >  1:975624/1‑100 (MQ=255)
              aTCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGaa                                                                             >  1:639081/1‑100 (MQ=255)
                 gCTTTTTCCCTTCAGGCATTTTTTTTTTTTTTTTTCAACGAAAGGCAGTTCCCAACAACAAAATAATGATGGGAGGTAAAAAATGAAACGGTGCGAAGTg                                                                          >  2:1194378/1‑100 (MQ=255)
                  cTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTgg                                                                         <  2:858759/100‑1 (MQ=255)
                    ttttCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCt                                                                       <  1:232655/100‑1 (MQ=255)
                       tCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTa                                                                    >  1:395152/1‑100 (MQ=255)
                         ccTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACt                                                                  >  1:914712/1‑100 (MQ=255)
                         ccTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACt                                                                  <  1:397750/100‑1 (MQ=255)
                         ccTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACt                                                                  >  1:333062/1‑100 (MQ=255)
                         ccTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACt                                                                  >  1:1002148/1‑100 (MQ=255)
                           ttCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTgg                                                                <  2:321699/100‑1 (MQ=255)
                            tCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGt                                                               <  1:603067/100‑1 (MQ=255)
                            tCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGt                                                               <  2:527760/100‑1 (MQ=255)
                             cAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTg                                                              >  2:1002761/1‑100 (MQ=255)
                               ggCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTTCCAACAACAAAATAAAGAAGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTgg                                                                <  2:1086952/96‑1 (MQ=255)
                               ggCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTg                                                              >  2:876043/1‑98 (MQ=255)
                                   tttttGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTa                                                        >  1:209160/1‑100 (MQ=255)
                                      ttGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGAAGGAAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATg                                                     >  2:525080/1‑100 (MQ=255)
                                        gTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGtt                                                   >  1:1238957/1‑100 (MQ=255)
                                           tAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGt                                                >  2:68753/1‑100 (MQ=255)
                                            aaTTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGt                                                >  2:58998/1‑99 (MQ=255)
                                             aTTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGttt                                              >  1:476013/1‑100 (MQ=255)
                                               tcaatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGc                                            <  1:273441/100‑1 (MQ=255)
                                                 aatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTg                                          <  1:104784/100‑1 (MQ=255)
                                                  atcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGt                                         >  1:804773/1‑100 (MQ=255)
                                                  atcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGt                                         >  2:393475/1‑100 (MQ=255)
                                                    caaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAt                                       <  1:1292853/100‑1 (MQ=255)
                                                     aaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAtt                                      >  1:1162070/1‑100 (MQ=255)
                                                         aaaGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAAc                                  >  1:887479/1‑100 (MQ=255)
                                                         aaaGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAAc                                  <  1:6223/100‑1 (MQ=255)
                                                          aaGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACt                                 >  2:610236/1‑100 (MQ=255)
                                                           aGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACtg                                <  2:639077/100‑1 (MQ=255)
                                                              cAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGa                             <  2:758723/100‑1 (MQ=255)
                                                                 ttCCCAACAACAAAATAAAGATGAGAAAAAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAagg                          <  2:1162059/100‑1 (MQ=255)
                                                                    ccAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAaggagg                       >  1:703860/1‑100 (MQ=255)
                                                                        caacaaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGc                   >  2:1115464/1‑100 (MQ=255)
                                                                            aaaaTAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATg               >  2:355016/1‑100 (MQ=255)
                                                                              aaTAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGAGATGGTAATGTTGGATTGCTGTATTGAACTGTGAAGGAGGAAGCCATGa              >  2:1293621/1‑99 (MQ=255)
                                                                                   aGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACttt        <  1:1002770/100‑1 (MQ=255)
                                                                                   aGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACttt        <  2:39678/100‑1 (MQ=255)
                                                                                    gATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACtttt       <  1:68754/100‑1 (MQ=255)
                                                                                    gATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAAAGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACtttt       >  2:123710/1‑100 (MQ=255)
                                                                                       gggAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAAt    >  1:876106/1‑100 (MQ=255)
                                                                                        ggAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATc   >  1:483757/1‑100 (MQ=255)
                                                                                        ggAGGTAAAAAACGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATc   >  1:859000/1‑100 (MQ=255)
                                                                                         gAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCg  <  1:1185756/100‑1 (MQ=255)
                                                                                               |                                                                                              
ATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCG  >  NC_000913/2563420‑2563607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: