Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 497,139 (T)5→6 intergenic (+145/‑36) htpG → / → adk protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase/adenylate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913497,1341.T96.8% 100.6 / ‑2.8 31intergenic (+140/‑41)htpG/adkprotein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase/adenylate kinase
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base T (10/20);  total (11/20)
Fisher's exact test for biased strand distribution p-value = 3.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

TTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCAG  >  NC_000913/497035‑497228
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tttcATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGc                                                                                                 >  1:33260/1‑100 (MQ=255)
tgtcatcaTCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGc                                                                                                 <  2:789553/98‑1 (MQ=255)
       atcTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttttttc                                                                                         >  2:675209/1‑98 (MQ=255)
       atcTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttttttc                                                                                         <  2:136111/100‑3 (MQ=255)
          tGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttttttcaaa                                                                                      >  1:1197020/1‑95 (MQ=255)
           gCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCaaaa                                                                                      <  2:155410/100‑1 (MQ=255)
                 tacGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCg                                                                                <  1:674014/98‑1 (MQ=255)
                 tCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCg                                                                                <  1:366584/100‑1 (MQ=255)
                   cGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGac                                                                              <  2:822189/100‑1 (MQ=255)
                   cGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGac                                                                              <  2:715933/100‑1 (MQ=255)
                          tATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACAtttt                                                                       <  2:27369/100‑1 (MQ=255)
                             ctcGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAg                                                                    <  1:767146/100‑1 (MQ=255)
                              tcGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAgg                                                                   >  1:847910/1‑100 (MQ=255)
                               cGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGATGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAggg                                                                  >  1:996197/1‑100 (MQ=255)
                                  cATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGAt                                                               >  1:843669/1‑100 (MQ=255)
                                       aCCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTATGGGGATTTTCg                                                          >  1:116007/1‑100 (MQ=255)
                                        ccGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGc                                                         <  1:326464/100‑1 (MQ=255)
                                             tCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCATTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGc                                                    <  2:1041668/100‑1 (MQ=255)
                                              cAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCg                                                   >  1:199348/1‑100 (MQ=255)
                                                  cccAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATc                                               <  1:209008/100‑1 (MQ=255)
                                                      ggTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTc                                           <  2:613498/100‑1 (MQ=255)
                                                         gCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGc                                        <  1:1215836/100‑1 (MQ=255)
                                                         gCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTTTTTTTTTTTACACATTTTAAGGGGATTTTCGCAATGCGTATCATTTTGCt                                       >  2:168798/1‑100 (MQ=255)
                                                           cTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCtt                                      <  2:175499/100‑1 (MQ=255)
                                                              tCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgc                                   <  1:539792/100‑1 (MQ=255)
                                                                  gAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTc                               >  2:375752/1‑100 (MQ=255)
                                                                      cAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCggg                           <  2:33259/100‑1 (MQ=255)
                                                                      cAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCggg                           >  1:269612/1‑100 (MQ=255)
                                                                           gCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCgg                      <  1:735909/100‑1 (MQ=255)
                                                                              tGTTGGTGGTATCGTTTATCGCTTTTTTTTTTTTTTTTTATACATTTTAAGGGGATTTTCTCAATGTGTATCATTCTGCTTGGCGCTCCGGGCGCtgg                     >  2:75937/1‑98 (MQ=255)
                                                                                ttGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGaaa                 <  2:477814/100‑1 (MQ=255)
                                                                                   gtggtATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCTCTCCGGGCGCGGGGAAAggg              <  2:794767/100‑1 (MQ=255)
                                                                                     ggtATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGAc            >  1:982986/1‑100 (MQ=255)
                                                                                              tATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCa   <  1:368275/100‑1 (MQ=255)
                                                                                              tATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCa   <  1:255191/100‑1 (MQ=255)
                                                                                               aTCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCAg  >  2:664350/1‑100 (MQ=255)
                                                                                                    |                                                                                              
TTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCAG  >  NC_000913/497035‑497228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: